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Issue: Vol. 5845 No. 1: 3 Jul. 2026
Type: Article
Published: 2026-07-03
DOI: 10.11646/zootaxa.5845.1.3
Page range: 52-86
Abstract views: 31
PDF downloaded: 0

The thistle flies of the genus Chaetostomella (Diptera: Tephritidae: Terelliini) from Morocco, with a reference to European and Asian fauna

Laboratory of Ecology; Systematics; and Conservation of Biodiversity; URL-CNRST N°18; FS; Abdelmalek Essaadi University; Tetouan; Morocco
I.I. Schmalhausen Institute of Zoology; National Academy of Sciences of Ukraine; Bogdan Khmelnytsky St. 15; 01054 Kyiv; Ukraine
Museum für Naturkunde; Invalidenstr. 43; 10115 Berlin; Germany
I.I. Schmalhausen Institute of Zoology; National Academy of Sciences of Ukraine; Bogdan Khmelnytsky St. 15; 01054 Kyiv; Ukraine; Museum für Naturkunde; Invalidenstr. 43; 10115 Berlin; Germany
Diptera new species host plant North Africa rif region phylogeny DNA barcoding Asteraceae Cardueae Carthamus Chamaeleon Cynara Mantisalca Ptilostemon

Abstract

The genus Chaetostomella Hendel, 1927 (Diptera: Tephritidae) in the fauna of Morocco is reviewed based on integrative morphological and molecular analyses. Phylogenetic reconstruction using a 657 COI barcoding sequences strongly supports the monophyly of the genus and resolves Chaetostomella steropea as the basalmost lineage, sister to the recently radiated Chaetostomella cylindrica species complex. Two species, Chaetostomella kitanea sp. nov. (reared from Cynara scolymus and Carthamus pectinatus), and Chaetostomella maroccana sp. nov. (reared from Mantisalca salmantica and Ptilostemon rhiphaeus) are described. Despite showing low genetic divergence from C. cylindrica, which indicates their evolutionary youth, both new species exhibit stable diagnostic morphological characters and geographic isolation. Chaetostomella steropea (Rondani, 1870) is redescribed based on specimens reared from Chamaeleon gummifer, representing its first record for North Africa. Tephritis algira Macquart, 1843 is considered a nomen dubium. A preliminary key to the species of Chaetostomella and remarks on the morphology, distribution and examined material of some little-known non-Moroccan species are provided.

References

  1. Agarwal, M.L. & Kapoor, V.C. (1982) Acanthiophilus helianthi (Rossi) and Chetostoma completum Kapoor et al. (Diptera: Tephritidae), serious pests of Centaurea cyanus Linnaeus (Compositae) in India. Journal of Entomological Research (New Delhi), 6, 102–104.
  2. Basov, V.M. (2000) A new species of tephritids of the genus Chaetostomella Hendel (Diptera, Tephritidae) from Russia. Entomologicheskoe Obozrenie, 79 (3), 734–739. [in Russian]
  3. Becker, T. (1905) Cyclorrhapha Schizophora: Holometopa. In: Becker, T., Bezzi, M., Kertész, K. & Stein, P. (Eds.), Katalog der paläarktischen Dipteren. Band IV. Wesselényi, Budapest, pp. 1–273. https://doi.org/10.5962/bhl.title.5143
  4. Chen, S.H. (1938) Subfamily Tephritinae. In: Zia, Y. & Chen, S.H. (Eds.), Trypetidae of North China. Sinensia, 9 (1–2), pp. 57–172.
  5. Coquillett, D.W. (1898) Report on a collection of Japanese Diptera, presented to the U.S. National Museum by the Imperial University of Tokyo. Proceedings of the U.S. National Museum, 21 (1146), 301–340. https://doi.org/10.5479/si.00963801.21-1146.301
  6. Coquillett, D.W. (1899) Diptera and Siphonaptera. In: Jordan, D.S. (Ed.), The Fur Seals and Fur-Seal Islands of the North Pacific Ocean. Pt. 4. Government Printing Office, Washington, pp. 341–346.
  7. Cumming, J.M. & Wood, D.M. (2017) 3. Adult morphology & Terminology. In: Kirk-Spriggs, A.H. & Sinclair, B.J. (Eds.), Manual of Afrotropical Diptera. Volume 1. South African National Biodiversity Institute, Pretoria, pp. 89–133.
  8. Diehl, S.R. & Bush, G.L. (1984) An evolutionary and applied perspective of insect biotypes. Annual Review of Entomology, 29, 471–504. https://doi.org/10.1146/annurev.ento.29.1.471
  9. Dirlbeková, O. (1982) Wirtspflanzen einiger Fruchtfliegenarten (Diptera, Tephritidae) in der Mongolei. Folia Facultatis Scientiarum Naturalium Universitatis Purkynianae Brunensis, 23, 41–44.
  10. Ebejer, M.J. (2015) The picture-winged flies and related families (Diptera, Tephritoidea) of the Maltese Islands. Bulletin of the Entomological Society of Malta, 7, 73–91.
  11. Ebejer, M.J. & Gatt, P. (2021) A checklist of the Diptera (Insecta) of the Maltese Islands. Zootaxa, 5018 (1), 1–75. https://doi.org/10.11646/zootaxa.5018.1.1
  12. Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32 (5), 1792–1797. https://doi.org/10.1093/nar/gkh340
  13. El Harym, Y., Belqat, B. & Korneyev, V.A. (2020) The fruit flies of Morocco: new records of the Tephritinae (Diptera, Tephritidae). Zoodiversity, 54 (6), 439–452. https://doi.org/10.15407/zoo2020.06.439
  14. El Harym, Y., Belqat, B. & Norrbom, A.L. (2022) Host plants of fruit flies (Diptera: Tephritidae) in Morocco. Zootaxa, 5196 (3), 355–387. https://doi.org/10.11646/zootaxa.5196.3.3
  15. Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3 (5), 294–299.
  16. Foote, R.H. (1984) Family Tephritidae. In: Soós, Á. & Papp, L. (Eds.), Catalogue of Palaearctic Diptera. Volume 9, Micropezidae — Agromyzidae. Akadémiai Kiádo, Budapest & Elsevier Science Publishers, Amsterdam, pp. 66–149.
  17. Freidberg, A. & Kugler, J. (1989) Fauna Palaestina, Insecta IV, Diptera: Tephritidae. The Israel Academy of Sciences and Humanities, Jerusalem, 212 pp.
  18. Geller, J., Meyer, C., Parker, M. & Hawk, H. (2013) Redesign of PCR primers for mitochondrial cytochrome c oxidase subunit I for marine invertebrates and application in all-taxa biotic surveys. Molecular Ecology Resources, 13 (5), 851–861. https://doi.org/10.1111/1755-0998.12138
  19. Hendel, F. (1927) 49. Trypetidae. In: Lindner, E. (Ed.), Die Fliegen der palaearktischen Region. Band 5. Schweizerbart, Stuttgart, pp. 1–221.
  20. Hering, E.M. (1936) Bohrfliegen aus der Mandschurei. (11. Beitrag zur Kenntnis der Trypetidae.). Konowia, 15, 180–189.
  21. Hering, E.M. (1939) Neue Trypetiden der Erde (25. Beitrag zur Kenntnis der Trypetiden). Verhandlungen des VII. Internationalen Kongresses für Entomologie (1938), 1, 165–190.
  22. Herrando-Moraira, S., Antonio Calleja, J., Galbany-Casals, M., Garcia-Jacas, N., Liu, J.-Q., López-Vinyallonga, S., Mandel, J.R., Massó, S., Montes-Moreno, N., Roquet, C., Sáez, L., Sennikov, A.N., Susanna, A. & Vilatersana, R. (2019) Nuclear and plastid DNA phylogeny of tribe Cardueae (Compositae) with Hyb-Seq data: A new subtribal classification and a temporal diversification framework. Molecular Phylogenetics and Evolution, 137, 313–332. https://doi.org/10.1016/j.ympev.2019.05.001
  23. Ito, S. (1984) Lieferung 5. In: Die japanischen Bohrfliegen. Maruzen Co., Ltd., Osaka, pp. 193–240.
  24. Kandybina, M.N. (1970) [On the larvae of fruit flies of the family Tephritidae (Diptera) inhabiting the heads of Compositae]. Entomologicheskoe Obozrenie, 49, 691–699. [in Russian]
  25. Kapoor, V.C., Malla, Y.K. & Ghosh, K. (1979) On a collection of fruit flies (Diptera: Tephritidae) from Kathmandu Valley, Nepal. Oriental Insects, 13, 81–85. https://doi.org/10.1080/00305316.1979.10433545
  26. Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16 (2), 111–120. https://doi.org/10.1007/BF01731581
  27. Knio, K.M., White, I.M. & Al-Zein, M.S. (2007) Host‐race formation in Chaetostomella cylindrica (Diptera: Tephritidae): Morphological and morphometric evidence. Journal of Natural History, 41 (25–28), 1697–1715. https://doi.org/10.1080/00222930701494486
  28. Korneyev, V.A. (1985) Fruit flies of the tribe Terelliini Hendel 1927 (Diptera, Tephritidae) of the fauna of the USSR. Entomologicheskoe Obozrenie, 64 (3), 626–644. [in Russian]
  29. Korneyev, V.A. (1987) Little-known species of the fruit flies (Diptera, Tephritidae) of the Ukrainian fauna. Fauna and biocoenotic connections of the insects of Ukraine. Naukova Dumka, Kiev, 83–87. [in Russian]
  30. Korneyev, V.A. (1999) Phylogeny of the subfamily Tephritinae: relationships of the tribes and subtribes. In: Aluja, M. & Norrbom, A.L. (Eds.), Fruit Flies (Tephritidae): Phylogeny and Evolution of Behavior. CRC Press, Boca Raton, pp. 549–580. https://doi.org/10.1201/9781420074468
  31. Korneyev, V.A. & Ovchinnikova, O.G. (2004) Fam. Tephritidae — Fruit Flies. In: Leley, A.S. (Ed.), Keys to the Insects of the Russian Far East. Vol. VI. Diptera and Fleas. Part 3. Dal’nauka, Vladivostok, pp. 456–564. [in Russian]
  32. Korlević, P., McAlister, E., Mayho, M., Makunin, A., Flicek, P. & Lawniczak, M.K. (2021) A minimally morphologically destructive approach for DNA retrieval and whole-genome shotgun sequencing of pinned historic dipteran vector species. Genome Biology and Evolution, 13 (10), evab226. https://doi.org/10.1093/gbe/evab226
  33. Kozub, D., Shapoval, Yu., Yatsenko, S., Starykh, V. & Dobarskiy, O. (2023) Helicon Focus 8.2.0. Pro. Available from: https://www.heliconsoft.com (accessed 8 October 2023)
  34. Loew, H. (1844) Kritische Untersuchung der europäischen Arten des Genus Trypeta Meig. Zeitschrift für die Entomologie (Germar), 5, 312–437.
  35. Loew, H. (1846) Fragmente zur Kenntniss der europäischen Arten einiger Dipterengattungen. Linnaea Entomologica, 1, 319–530.
  36. Macquart, J.P.M. (1835) Histoire naturelle des Insectes. Diptères. Tome deuxième. N. E. Roret, Paris, 703 pp. https://doi.org/10.5962/bhl.title.14274
  37. Macquart, J.P.M. (1843) Diptères exotiques nouveaux ou peu connus [2(3)]. Mémoires de la Société Royale des Sciences, de l’Agriculture et des Arts de Lille, 1842, 162–460.
  38. Maneesh, P.S. & Hancock, D.L. (2025) Five new species of subfamily Tephritinae (Tephritidae: Diptera) from Himalayas and description of postabdominal structures in Chaetostomella completa (Kapoor, Malla & Ghosh), Pliomelaena spathuliniforma (Dirlbek & Dirlbek) and P. zonogastra (Bezzi). Zootaxa, 5689 (2), 299–332. https://doi.org/10.11646/zootaxa.5689.2.4
  39. Merz, B. (1994) Diptera: Tephritidae. Insecta Helvetica Fauna. 10. HGE press, Geneva, 198 pp.
  40. Merz, B. (2000) Two new Tephritidae (Diptera) from the Western Palaearctic region. Revue Suisse de Zoologie, 107 (4), 883–892. https://doi.org/10.5962/bhl.part.80153
  41. Mihályi, F. (1960) Fúrólegyek. Trypetidae. Magyarország Állatvilága, 15 (3), 1–76.
  42. Neuenschwander, P. & Freidberg, A. (1983) The fruit flies of Crete (Diptera: Tephritidae). Israel Journal of Entomology, 17, 81–94.
  43. Norrbom, A.L., Carroll, L.E., Thompson, R.C., White, I.M. & Freidberg, A. (1999) Systematic database of names. In: Thompson, F.C. (Ed.), Fruit Fly Expert Identification System and Systematic Information Database. Myia, 9, pp. 65–251.
  44. Richter, V.A. (1970) Tephritidae (Trypetidae) — pestrokrylki [Family Tephritidae (Trypetidae) — fruit flies]. In: Bei-Bienko, G.Ya. (Ed.), Opredelitel’ nasekomykh evropeyskoy chasti SSSR. Tom V. Dvukrylyye, blokhi. Chast’ 2. Nauka, Moscow & Leningrad, pp. 132–172. [in Russian]
  45. Richter, V.A. (1975) On the fauna of fruit-flies (Diptera, Tephritidae) of the Mongolian People’s Republic. I. Nasekomye Mongolii, 3 (1), 582–602. [in Russian]
  46. Robineau-Desvoidy, J.B. (1830) Essai sur les Myodaires. Mémoires présentés par divers savants à l’Académie Royale des Sciences de l’Institut de France, 2, 1–813. https://doi.org/10.5962/bhl.title.8552
  47. Rondani, C. (1870) Ortalidinae Italicae collectae, distinctae et in ordinum dispositae. Dipterologiae Italicae prodromus. Pars VII. Fasc. 4 (sect. 1) [concl.]. Bollettino della Società Entomologica Italiana, 2, 105–133.
  48. Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61 (3), 539–542. https://doi.org/10.1093/sysbio/sys029
  49. Shiraki, T. (1933) A systematic study of Trypetidae in the Japanese Empire. Memoirs of the Faculty of Science and Agriculture, Taihoku Imperial University, 8 (Entomology 2), 1–509.
  50. Smith, C.A., Al-Zein, M.S., Sayar, N.P. & Knio, K.M. (2009) Host races in Chaetostomella cylindrica (Diptera: Tephritidae): genetic and behavioural evidence. Bulletin of Entomological Research, 99 (4), 425–432. https://doi.org/10.1017/s0007485308006482
  51. Srivathsan, A., Hartop, E., Puniamoorthy, J., Lee, W.T., Kutty, S.N., Kurina, O. & Meier, R. (2019) Rapid, large-scale species discovery in hyperdiverse taxa using 1D MinION sequencing. BMC Biology, 17 (1), 96. https://doi.org/10.1186/s12915-019-0706-9
  52. Srivathsan, A., Lee, L., Katoh, K., Hartop, E., Kutty, S.N., Wong, J., Yeo, D. & Meier, R. (2021) ONTbarcoder and MinION barcodes aid biodiversity discovery and identification by everyone, for everyone. BMC Biology, 19 (1), 217. https://doi.org/10.1186/s12915-021-01141-x
  53. Srivathsan, A., Feng, V., Suárez, D., Emerson, B. & Meier, R. (2024) ONTbarcoder 2.0: rapid species discovery and identification with real‐time barcoding facilitated by Oxford Nanopore R10.4. Cladistics, 40 (2), 192–203. https://doi.org/10.1111/cla.12571
  54. Steck, G.J. (1984) Chaetostomella undosa (Diptera: Tephritidae): biology, ecology, and larval description. Annals of the Entomological Society of America, 77, 669–678. https://doi.org/10.1093/aesa/77.6.669
  55. Tamura, K., Stecher, G. & Kumar, S. (2021) MEGA 11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution, 38 (7), 3022–3027. https://doi.org/10.1093/molbev/msab120
  56. Varley, G.C. (1937) The life history of some trypetid flies with descriptions of the early stages (Diptera). Proceedings of the Royal Entomological Society of London, Series A, General Entomology, 12, 109–122. https://doi.org/10.1111/j.1365-3032.1937.tb00465.x
  57. Wang, X.-J. (1998) The fruit flies (Diptera: Tephritidae) of the East Asian Region. Acta Zootaxonomica Sinica, Supplement 21 (1996), [1­6] + 1–338 + [1–40] + I–XL.
  58. White, I.M. (1988) Tephritid flies: Handbooks for the identification of British insects. Vol. 10. Part 5a. Royal Entomological Society of London, London, 134 pp.
  59. White, I.M. & Elson-Harris, M.M. (1992) Fruit flies of economic significance: their identification and bionomics. International Institute of Entomology, London, xii + 601 pp. https://doi.org/10.1079/9780851987903.0000
  60. White, I.M. & Marquardt, K. (1989) A revision of the genus Chaetorellia Hendel (Diptera: Tephritidae) including a new species associated with spotted knapweed, Centaurea maculosa Lam. (Asteraceae). Bulletin of Entomological Research, 79, 453–487. https://doi.org/10.1017/s0007485300018459
  61. White, I.M., Headrick, D.H., Norrbom, A.L. & Carroll, L.E. (1999) Glossary. In: Aluja, M. & Norrbom, A.L. (Eds.), Fruit Flies (Tephritidae): Phylogeny and Evolution of Behavior. CRC Press, Boca Raton, pp. 881–924. https://doi.org/10.1201/9781420074468.sec8
  62. Wiedemann, C.R.W. (1830) Aussereuropäische zweiflügelige Insekten. Vol. 2. Schulz, Hamm, xii + 684 pp.
  63. Zhang, D., Gao, F., Jakovlić, I., Zou, H., Zhang, J., Li, W.X. & Wang, G.T. (2020) PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources, 20 (1), 348–355. https://doi.org/10.1111/1755-0998.13117
  64. Zwölfer, H. (1965) Preliminary list of phytophagous insects attacking wild Cynareae (Compositae) species in Europe. Technical Bulletin of the Commonwealth Institute of Biological Control, 6, 81–154.

How to Cite

Harym, Y.E., Troshyn, A.M., Schreiber, R. & Korneyev, V.A. (2026) The thistle flies of the genus Chaetostomella (Diptera: Tephritidae: Terelliini) from Morocco, with a reference to European and Asian fauna. Zootaxa, 5845 (1), 52–86. https://doi.org/10.11646/zootaxa.5845.1.3