Abstract
A new species in the Platycerus bashanicus species group, Platycerus dabiemontanus Zhang & Kubota, sp. nov., is described from Anhui Province, China. The molecular phylogeny based on mitochondrial COI and nuclear ITS2 sequences indicates a close relationship of this new species with P. yeren Imura, 2008 and P. canae Imura, 2010. Significant mito-nuclear discordance observed between populations suggests the occurrence of mitochondrial gene introgression. This study extends the eastern distribution limit of the P. bashanicus group by 500 km and represents the first record of the genus Platycerus Geoffroy, 1762 from Anhui Province.
References
- Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: More models, new heuristics and parallel computing. Nature Methods, 9, 772–772. https://doi.org/10.1038/nmeth.2109
- Hawlitschek, O., Ortiz, E.M., Noori, S., Webster, K.C., Husemann, M. & Pereira, R.J. (2022) Transcriptomic data reveals nuclear-mitochondrial discordance in Gomphocerinae grasshoppers (Insecta: Orthoptera: Acrididae). Molecular Phylogenetics and Evolution, 170, 107439. https://doi.org/10.1016/j.ympev.2022.107439
- Huang, H. & Chen, C.-C. (2017) Stag beetles of China 3. Formosa Ecological Company, Taipei, 524 pp. [in English and Chinese]
- Huang, H. & Chen, C.-C. (2022) New or little known stag beetles of the genus Platycerus Geoffroy, 1762 from China (Coleoptera: Lucanidae). Beetles World, 24, 24–29.
- Imura, Y. (2010a) The genus Platycerus of East Asia. Roppon-Ashi Entomological Books, Tokyo, 240 pp. [in Japanese, partly in English and Chinese]
- Imura, Y. (2010b) A new Platycerus (Coleoptera, Lucanidae) from the Baotianman Nature Reserve in Western Henan, central China. Elytra, 38, 227–232.
- Imura, Y. (2012) Description of a new Platycerus (Coleoptera, Lucanidae) from Sichuan, Southwest China, with records of two other species. Elytra, New series, 2, 61–68.
- Imura, Y. & Nagahata, Y. (2009) Phylogeny and evolution of the tribe Platycerini (Coleoptera, Lucanidae) of the world inferred from mitochondrial 16S rRNA and COI gene sequences. Elytra, 37, 261–272.
- Jing, K.-X., Wang, Y.-F. & Huang, Y.-Z. (2026) A new species of the genus Platycerus Geoffroy, 1762 (Coleoptera: Lucanidae: Lucaninae) from Sichuan, China. The Indochina Entomologist, 2 (1), 1–13. https://doi.org/10.70590/ice.2026.02.01
- Kamvar, Z.N., Tabima, J.F. & Grünwald, N.J. (2014) Poppr: an R package for genetic analysis of populations with clonal, partially clonal, and/or sexual reproduction. PeerJ, 2, e281. https://doi.org/10.7717/peerj.281
- Katoh, K., Misawa, K., Kuma, K.I. & Miyata, T. (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research, 30 (14), 3059–3066. https://doi.org/10.1093/nar/gkf436
- Kolde, R. (2018) Package ‘pheatmap’: Pretty heatmaps, version 1.0.12. R package. Available from: https://github.com/raivokolde/pheatmap (accessed 19 December 2024)
- Kubota, K., Kubota, N. & Otobe, H. (2008) A revision of Platycerus acuticollis Y. Kurosawa (Coleoptera, Lucanidae) and its closely related species. Biogeography, 10, 79–102.
- Kubota, K., Kubota, N., Otobe, H. & Nagahata Y. (2009) A morphological phylogeny of the genus Platycerus (Coleoptera, Lucanidae) in Japan. Biogeography, 11, 57–72.
- Kubota, K., Nagahata, Y., Ikeda, H., Kubota, N., Otobe, H. & Umetsu, K. (2011) Diversification process of stag beetles belonging to the genus Platycerus Geoffroy (Coleoptera: Lucanidae) in Japan based on nuclear and mitochondrial genes. Entomological Science, 14, 411–427. https://doi.org/10.1111/j.1479-8298.2011.00466.x
- Kubota, K, Zhu, X.-J., Ma, T. & Wen, X.-J. (2018) A new species of the Genus Platycerus Geoffroy (Coleoptera, Lucanidae) from the Qinling Mountains, Shaanxi Province, China. Elytra, New Series, 8, 175–178.
- Paradis, E., Blomberg, S., Bolker, B., Brown, J., Claude, J., Cuong, H.S., Desper, R. & Didier, G. (2019) Package ‘ape’: Analyses of phylogenetics and evolution, version 5.3. R package. Available from: https://CRAN.R-project.org/package=ape (accessed 19 December 2024)
- Rambaut, A. (2019) FigTree. Available from: http://tree.bio.ed.ac.uk/software/figtree (accessed September 6, 2024)
- Rambaut, A., Drummond, A.J., Xie, D., Baele, G. & Suchard, M.A. (2018) Posterior summarization in Bayesian phylogenetics using Tracer 1.7. Systematic Biology, 67, 901–904. https://doi.org/10.1093/sysbio/syy032
- Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Hohna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542. https://doi.org/10.1093/sysbio/sys029
- Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America, 87, 651–701. https://doi.org/10.1093/aesa/87.6.651
- Stamatakis, A. (2016) The RAxML v8. 2. X Manual. Heidleberg Institute for Theoretical Studies. Available from: https://cme.h-its.org/exelixis/resource/download/NewManual.pdf (accessed 6 September 2024)
- Stull, G.W., Soltis, P.S., Soltis, D.E., Gitzendanner, M.A. & Smith, S.A. (2020) Nuclear phylogenomic analyses of asterids conflict with plastome trees and support novel relationships among major lineages. American Journal of Botany, 107, 790-805. https://doi.org/10.1002/ajb2.1468
- Tanahashi, M. & Hawes, C.J. (2016) The presence of a mycangium in the horned stag beetle Sinodendron cylindricum (Coleoptera: Lucanidae) and the associate yeast symbionts. Journal of Insect Science, 16, pii76. https://doi.org/10.1093/jisesa/iew054
- Wang, Y., Zhu, X., Dong, X., Wang, K., Yang, R., Zheng, C. & Bu, W. (2025) Species boundaries and phylogenetics of Aelia stink bugs (Hemiptera: Pentatomidae) in China, with notes on mito‐nuclear discordance. Zoologica Scripta, 54, 196–212. https://doi.org/10.1111/zsc.12706
- Zhang, S.-N. & Kubota, K. (2022) Local ecological divergence of two closely related stag beetles based on genetic, morphometric, and environmental analyses. Ecology and Evolution, 12, e8837. https://doi.org/10.1002/ece3.8837
- Zhang, S.-N. & Kubota, K. (2023) Strongly exclusive distribution with putative character displacement in two flying stag beetles. Evolution, 77, 155–165.
- https://doi.org/10.1093/evolut/qpac034
- Zhu, X.-J., Ma, T., Imura, Y., Wen, X.-J. & Kubota, K. (2020) Molecular phylogeny and historical biogeography of the genus Platycerus (Coleoptera, Lucanidae) in East Asia. Zoologica Scripta, 49, 582–595. https://doi.org/10.1111/zsc.12429
