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Type: Article
Published: 2025-06-11
Page range: 501-526
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Taxonomic assessment of Javanese Kalophrynus Tschudi, 1838, with a description of a new species (Amphibia, Anura, Microhylidae)

Laboratory of Herpetology; Museum Zoologicum Bogoriense; Research Center for Biosystematics and Evolution; National Research and Innovation Agency (BRIN); Gd. Widyasatwaloka; KST Soekarno; Jl. Raya Jakarta Bogor km 46; Cibinong; Bogor; West Java 16912; Indonesia; Graduate Program of Animal Bioscience; Department of Biology; Faculty of Mathematics and Natural Sciences; Bogor Agricultural University; Bogor; West Java 16680; Indonesia
Amfibi Reptil Sumatra; Bogor; West Java 16100; Indonesia
Graduate Program of Animal Bioscience; Department of Biology; Faculty of Mathematics and Natural Sciences; Bogor Agricultural University; Bogor; West Java 16680; Indonesia
Amfibi Reptil Sumatra; Bogor; West Java 16100; Indonesia
Amfibi Reptil Sumatra; Bogor; West Java 16100; Indonesia.
Laboratory of Herpetology; Museum Zoologicum Bogoriense; Research Center for Biosystematics and Evolution; National Research and Innovation Agency (BRIN); Gd. Widyasatwaloka; KST Soekarno; Jl. Raya Jakarta Bogor km 46; Cibinong; Bogor; West Java 16912; Indonesia
Herpetological Society of Indonesia; Bogor; West Java 16680; Indonesia
Graduate Program of Animal Bioscience; Department of Biology; Faculty of Mathematics and Natural Sciences; Bogor Agricultural University; Bogor; West Java 16680; Indonesia
Amphibia Kalophrynus java morphology 16s rrNA gene bioacoustics

Abstract

Previous studies showed that the Javanese Kalophrynus comprises two species: K. minusculus and K. pleurostigma. However, the taxonomic relationship between these species remains unresolved. To clarify this, we conducted detailed comparisons through molecular, morphological characteristics, and bioacoustics analyses of Javanese Kalophrynus. Molecular analyses revealed that Javanese K. pleurostigma and K. minusculus formed a clade. Morphologically and bioacoustically, Javanese K. pleurostigma is distinct from K. minusculus and other Kalophrynus species. Therefore, we describe the Javanese lineage of K. pleurostigma as a new species, Kalophrynus misbahulmuniri sp. nov. Additionally, we provide a comprehensive redescription of the topotypic population of K. minusculus based on new material. This study contributes to a better understanding of Indonesian amphibians, especially Kalophrynus diversity in Java.

 

References

  1. Arifin, U., Smart, U., Hertwig, S.T., Smith, E.N., Iskandar, D.T. & Haas, A. (2018) Molecular phylogenetic analysis of a taxonomically unstable ranid from Sumatra, Indonesia, reveals a new genus with gastromyzophorous tadpoles and two new species. Zoosystematics and Evolution, 94, 163–193. https://doi.org/10.3897/zse.94.22120
  2. Chan, K.O. & Grismer, L.L. (2022) GroupStruct: an R package for allometric size correction. Zootaxa, 5124 (4), 471–482. https://doi.org/10.11646/zootaxa.5124.4.4
  3. Chan, K.O., Grismer, L.L. & Grismer, J.L. (2011) A new insular, endemic frog of the genus Kalophrynus Tschudi, 1838 (Anura: Microhylidae) from Tioman Island, Pahang, Peninsular Malaysia. Zootaxa, 3123 (1), 60–68. https://doi.org/10.11646/zootaxa.3123.1.4
  4. Dehling, J.M. (2011) A new karst-dwelling species of Kalophrynus (Anura: Microhylidae) from Gunung Mulu National Park, Borneo, Malaysia. Zootaxa, 2737 (1), 49–60. https://doi.org/10.11646/zootaxa.2737.1.4
  5. Devenish, C., Junaid, A.R., Saryanthi, R., van Balen, S.B., Kaprawi, F., Aprianto, G.C., Stanley, R.C., Poole, O., Owen, A., Collar, N.J. & Marsden, S.J. (2021) Biological richness of Gunung Slamet, Central Java, and the need for its protection. Oryx, 56 (3), 429–438. https://doi.org/10.1017/S0030605320001222
  6. Dring, J. (1983) Some new frogs from Sarawak. Amphibia-Reptilia, 4, 103–115. https://doi.org/10.1163/156853883X00021
  7. Dutta, S.K., Ahmed, M.F. & Das, I. (2000) Kalophrynus (Anura: Microhylidae), a new genus for India, with the description of a new species, Kalophrynus orangensis, from Assam State. Hamadryad, 25, 67–74.
  8. Frost, D.R. (2024) Amphibian Species of the World: an Online Reference. Version 6.1. American Museum of Natural History, New York, Electronic Database accessible. Available from: https://amphibiansoftheworld.amnh.org/index.php (accessed 14 December 2024)
  9. Fukuyama, I., Matsui, M., Eto, K., Hossman, M.Y. & Nishikawa, K. (2021) Discovery of a deeply divergent and highly endemic frog lineage from Borneo: A taxonomic revision of Kalophrynus nubicola Dring, 1983 with descriptions of two new species (Amphibia: Anura: Microhylidae). Zoologischer Anzeiger, 293, 326–343. https://doi.org/10.1016/j.jcz.2021.06.016
  10. Gonggoli, A.D., Munir, M., Kaprawi, F., Kirschey, T. & Hamidy, A. (2024) A new species of tree frog (Amphibia, Anura, Rhacophoridae) from Central Java, Indonesia. Raffles Bulletin of Zoology, 72, 219–234. https://doi.org/10.26107/RBZ-2024-0019
  11. Hamidy, A., Munir, M., Mumpuni, M., Rahmania, M. & Kholik, A.A. (2018) Detection of cryptic taxa in the genus Leptophryne (Fitzinger, 1843) (Amphibia: Bufonidae) and the description of a new species from Java, Indonesia. Zootaxa, 4450 (4), 427–444. https://doi.org/10.11646/zootaxa.4450.4.2
  12. Hedges, S.B., Nussbaum, R.A. & Maxson, L.R. (1993) Caecilian phylogeny and biogeography inferred from mitochondrial DNA sequences of the 12S rRNA and 16S rRNA genes (Amphibia: Gymnophiona). Herpetological Monographs, 7, 64–76. https://doi.org/10.2307/1466952
  13. Inger, R.F. (1966) The systematics and zoogeography of the Amphibia of Borneo. Fieldiana Zoology, 52, 1–402. https://doi.org/10.5962/bhl.title.3147
  14. Inger, R.F., Stuebing, R.B., Grafe, T.U. & Dehling, J.M. (2017) A Field Guide to the Frogs of Borneo. 3rd Edition. Natural History Publications (Borneo), Kota Kinabalu, 236 pp.
  15. Iskandar, D.T. (1998) The amphibians of Java and Bali. Research and Development Centre for Biology LIPI and GEF Biodiversity Collections Project, Bogor, xix + 177 pp.
  16. Kaiser, H.F. (1960) The application of electronic computers to factor analysis. Educational and Psychological Measurement, 20, 141–151. https://doi.org/10.1177/001316446002000116
  17. Kaprawi, F., Alhadi, F., Basalamah, F., Noerwana, O., Kirschey, T., Setia, T.M. & Hamidy. (2022a) Amphibians of Leuweung Sancang Nature Reserve, West Java, Indonesia. Zoo Indonesia, 31, 43–54.
  18. Kaprawi, F., Noerwana, O., Setia, T.M., Kirschey, T. & Hamidy, A. (2022b) Herpetofauna Cagar Alam Leuweung Sancang. Amfibi Reptil Sumatra, Bogor, 61 pp.
  19. Kassambara, A. & Mundt, F. (2022) Package ‘factoextra’. Available from: https://cran.r-project.org/web/packages/factoextra/factoextra.pdf (accessed 14 December 2024)
  20. Kiew, B.H. (1984) A new species of sticky frog (Kalophrynus palmatissimus n. sp.) from Peninsular Malaysia. Malayan Nature Journal, 37, 145–152.
  21. Köhler, J., Jansen, M., Rodriguez, A., Kok, P.J., Toledo, L.F., Emmrich, M., Glaw, F., Haddad, C.F.B., Rödel, M.O. & Vences, M. (2017) The use of bioacoustics in anuran taxonomy: theory, terminology, methods and recommendations for best practice. Zootaxa, 4251 (1), 1–124. https://doi.org/10.11646/zootaxa.4251.1.1
  22. Kurniawan, N., Septiadi, L., Fathoni, M., Kadafi, A.M. & Marhendra, A.P.W. (2022) A Checklist of the Herpetofauna of Nusa Kambangan Island, Central Java, Indonesia. Tropical Life Sciences Research, 33 (2), 91. https://doi.org/10.21315/tlsr2022.33.2.6
  23. Leaché, A.D. & Reeder, T.W. (2002) Molecular systematics of the eastern fence lizard (Sceloporus undulatus): a comparison of parsimony, likelihood, and Bayesian approaches. Systematic Biology, 51, 44–68. https://doi.org/10.1080/106351502753475871
  24. Lleonart, J., Salat, J. & Torres, G.J. (2000) Removing allometric effects of body size in morphological analysis. Journal of Theoretical Biology, 205, 85–93. https://doi.org/10.1006/jtbi.2000.2043
  25. Lohman, D.J., de Bruyn, M., Page, T., Rintelen, K.V., Hall, R., Ng, P.K.L., Hsi-Te, S., Carvalho, G.R. & Rintelen, V.T. (2011) Biogeography of the Indo-Australian archipelago. Annual Review of Ecology, Evolution, and Systematics, 42, 205–226. https://doi.org/10.1146/annurev-ecolsys-102710-145001
  26. Malkmus, R. & Riede, K. (1996) Zur Bioakustik von Megophrys baluensis (Boulenger, 1899) und Kalophrynus baluensis Kiew, 1984, zweier seltener Froscharten aus Borneo (Anura: Pelobatidae, Microhylidae). Herpetozoa, 9, 151–155.
  27. Matsui, M. & Nishikawa, K. (2011) A new tiny Kalophrynus (Amphibia, Anura, Microhylidae) from northern Sarawak, Malaysian Borneo. Current Herpetology, 30, 145–153. https://doi.org/10.5358/hsj.30.145
  28. Matsui, M. (1984) Morphometric variation analyses and revision of the Japanese toads (Genus Bufo, Bufonidae). Contributions from the Biological Laboratory, Kyoto University, 26, 209–428.
  29. Matsui, M. (1996) Distinct specific status of Kalophrynus pleurostigma interlineatus (Anura, Microhylidae). Copeia, 1996, 440–445. https://doi.org/10.2307/1446861
  30. Matsui, M. (2009) A new species of Kalophrynus with a unique male humeral spine from Peninsular Malaysia (Amphibia, Anura, Microhylidae). Zoological Science, 26, 579–585. https://doi.org/10.2108/zsj.26.579
  31. Matsui, M., Eto, K., Belabut, D.M. & Nishikawa, K. (2017) A new Kalophrynus (Amphibia, Anura, Microhylidae) from Peninsular Malaysia. Current Herpetology, 36, 75–86. https://doi.org/10.5358/hsj.36.75
  32. Matsui, M., Nishikawa, K., Belabut, D.M., Norhayati, A. & Yong, H.S. (2012) A new species of Kalophrynus (Amphibia, Anura, Microhylidae) from Southern Peninsular Malaysia. Zootaxa, 3155 (1), 38–46. https://doi.org/10.11646/zootaxa.3155.1.4
  33. McLeod, D.S., Horner, S.J., Husted, C., Barley, A. & Iskandar, D. (2011) “Same-same, but different”: an unusual new species of the Limnonectes kuhlii complex from West Sumatra (Anura: Dicroglossidae). Zootaxa, 2883 (1), 52–64. https://doi.org/10.11646/zootaxa.2883.1.4
  34. Mertens, R. (1957) Amphibien und reptilien aus dem äusserstenWesten Javas und von benachbarten eilanden. Treubia, 24, 83–105. https://doi.org/10.14203/treubia.v24i1.2719
  35. Minh, B.Q., Nguyen, M.A.T. & Haeseler, A. (2013) Ultrafast Approximation for phylogenetic bootstrap. Molecular Biology and Evolution, 30 (5), 1188–1195. https://doi.org/10.1093/molbev/mst024
  36. Munir, M., Hamidy, A., Farajallah, A. & Smith, E.N. (2018) A new Megophrys Kuhl and van Hasselt (Amphibia: Megophryidae) from southwestern Sumatra, Indonesia. Zootaxa, 4442 (3), 389–412. https://doi.org/10.11646/zootaxa.4442.3.3
  37. Munir, M., Nishikawa, K., Hamidy, A. & Smith, E.N. (2021) Two new species of Megophrys Kuhl and Van Hasselt (Amphibia: Megophryidae) from Sumatra, Indonesia. Zootaxa, 5057 (4), 503–529. https://doi.org/10.11646/zootaxa.5057.4.3
  38. O'Connell, K.A., Hamidy, A., Kurniawan, N., Smith, E.N. & Fujita, M.K. (2018) Synchronous diversification of parachuting frogs (Genus Rhacophorus) on Sumatra and Java. Molecular Phylogenetics and Evolution, 123, 101–112. https://doi.org/10.1016/j.ympev.2018.02.003
  39. Ohler, A. & Grosjean, S. (2005) Color pattern and call variation in Kalophrynus from south-east Asia. Herpetozoa, 18, 99–106.
  40. R Core Team (2020) R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna. Available from: https://www.R-project.org (accessed 14 December 2024)
  41. Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Hohna, S., Larget, B., Liu, L., Suchard, M.A. & Hulsenbeck, J.P. (2012) MRBAYES 3.2: Efficient Bayesian phylogenetic inference and model selection across a large model space. Systematic Biology, 61, 539–542. https://doi.org/10.1093/sysbio/sys029
  42. Santoro, A., Piras, F. & Yu, Q. (2023) Spatial analysis of deforestation in Indonesia in the period 1950–2017 and the role of protected areas. Biodiversity and Conservation, 1–27. [published online] https://doi.org/10.1007/s10531-023-02679-8
  43. Savage, J.M. (1975) Systematics and distribution of the Mexican and Central American stream frogs related to Eleutherodactylus rugulosus. Copeia, 1975, 254–306. https://doi.org/10.2307/1442883
  44. Tanabe, A.S. (2011) Kakusan4 and Aminosan: two programs for comparing nonpartitioned, proportional and separate models for combined molecular phylogenetic analyses of multilocus sequence data. Molecular Ecology Resources, 11, 914–921. https://doi.org/10.1111/j.1755-0998.2011.03021.x
  45. Thorpe, R.S. (1983) A review of the numerical methods for recognising and analysing racial differentiation. In: Felsenstein, J. (Ed.), Numerical Taxonomy. Springer Verlag, Berlin and Heidelberg, pp. 404–423. https://doi.org/10.1007/978-3-642-69024-2_43
  46. Trifinopoulos, J., Nguyen, L.T., von Haeseler, A. & Minh, B.Q. (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research, 44 (W1), W232–W235. https://doi.org/10.1093/nar/gkw256
  47. Tschudi, J.J.V. (1838) Classification der Batrachier mit Berücksichtigung der fossilen Thiere dieser Abtheilung der Reptilien. Mémoires de la Société des sciencesnaturelles de Neuchâtel, 2, 1–99. https://doi.org/10.5962/bhl.title.4883
  48. Vassilieva, A.B., Galoyan, E.A., Gogoleva, S.S. & Poyarkov Jr, N.A. (2014) Two new species of Kalophrynus Tschudi, 1838 (Anura: Microhylidae) from the Annamite mountains in southern Vietnam. Zootaxa, 3796 (3), 401–434. https://doi.org/10.11646/zootaxa.3796.3.1
  49. Zug, G.R. (2015) Morphology and systematics of Kalophrynus interlineatus-pleurostigma populations (Anura: Microhylidae: Kalophryninae) and a taxonomy of the genus Kalophrynus Tschudi, Asian sticky frogs. Proceedings of the California Academy of Sciences, 62, 135–190.

How to Cite

Gonggoli, A.D., Fauzan, M.F., Kaprawi, F., Herlambang, A.E.N., Kirono, S., Wiradarma, H., Alif, H. & Midy, A.H. (2025) Taxonomic assessment of Javanese Kalophrynus Tschudi, 1838, with a description of a new species (Amphibia, Anura, Microhylidae). Zootaxa, 5646 (4), 501–526. https://doi.org/10.11646/zootaxa.5646.4.2