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Issue: Vol. 5636 No. 1: 15 May 2025
Type: Article
Published: 2025-05-15
DOI: 10.11646/zootaxa.5636.1.6
Page range: 144-162
Abstract views: 237
PDF downloaded: 6

Description of a new Eratigena Bolzern, Burckhardt & Hänggi, 2013 (Arachnida: Araneae: Agelenidae) from the Balkans based on an integrative approach

Department of Biology; Faculty of Mathematics and Natural Sciences; University of Prishtina; Mother Teresa street p.n.; 10000 Prishtina; Republic of Kosovo
Department of Biology; Faculty of Mathematics and Natural Sciences; University of Prishtina; Mother Teresa street p.n.; 10000 Prishtina; Republic of Kosovo
Institute of Biodiversity and Ecosystem Research; Bulgarian Academy of Sciences; 1 Tsar Osvoboditel Blvd.; 1000 Sofia; Bulgaria
University of Peja “Haxhi Zeka”; Faculty of Agribusiness; Street “UÇK” 30000 Pejë; Republic of Kosovo
Institute of Biology; University of Graz; Universitätsplatz 2;8010 Graz; Austria
Faculty of Agribusiness; University of Peja “Haxhi Zeka”; Street “UÇK” 30000 Pejë; Republic of Kosovo
Department of Biology; Faculty of Mathematics and Natural Sciences; University of Prishtina; Mother Teresa street p.n.; 10000 Prishtina; Republic of Kosovo
Faculty of Agribusiness; University of Peja “Haxhi Zeka”; Street “UÇK” 30000 Pejë; Republic of Kosovo
Faculty of Agribusiness; University of Peja “Haxhi Zeka”; Street “UÇK” 30000 Pejë; Republic of Kosovo; Department of Biology; University of Antwerp; Groenenborgerlaan 171; 2020 Antwerp; Belgium; Faculty of Civil Engineering; University of Rijeka; Radmile Matejcic 3; 51000; Rijeka; Croatia
Araneae Barcoding COI Eratigena agrestis Kosovo new species

Abstract

New data on agelenid spiders of the Balkans are provided. A new species of the genus Eratigena Bolzern, Burckhardt & Hänggi, 2013, Eratigena mirusha sp. nov. (♂/♀) from the Koznik Mountain in Kosovo is described, based on molecular and morphological data. The males Eratigena mirusha sp. nov. are most similar to E. agrestis (Walckenaer) but possess differently shaped conductor and retrolateral tibial apophysis. Females of both species differ in the shape of the epigyne and the posterior sclerite and from the positions of the copulatory openings, and the epigynal pseudo teeth. The genetic divergence calculated based on the barcode region of the cytochrome c oxidase subunit I gene (COI) confirms the morphological distinctness and supports Eratigena mirusha sp. nov. as a sister species of E. agrestis.

 

References

  1. Arabi, J., Judson, M.L., Deharveng, L., Lourenço, W.R., Cruaud, C. & Hassanin, A. (2012) Nucleotide composition of CO1 sequences in Chelicerata (Arthropoda): detecting new mitogenomic rearrangements. Journal of Molecular Evolution, 74 (1), 81–95. https://doi.org/10.1007/s00239-012-9490-7
  2. Ashfaq, M., Blagoev, G., Tahir, H.M., Khan, A.M., Mukhtar, M.K., Akhtar, S. & Hebert, P.D. (2019) Assembling a DNA barcode reference library for the spiders (Arachnida: Araneae) of Pakistan. PLoS ONE, 14 (5), e0217086. https://doi.org/10.1371/journal.pone.0217086
  3. Astrin, J.J., Höfer, H., Spelda, J., Holstein, J., Bayer, S., Hendrich, L., Huber, B.A., Kielhorn, K.H., Krammer, H.J., Lemke, M., Monje, J.C., Morinière, J., Rulik, B., Petersen, M., Janssen, H. & Muster, C. (2016) Towards a DNA Barcode Reference Database for Spiders and Harvestmen of Germany. PLoS ONE, 11 (9), e0162624. https://doi.org/10.1371/journal.pone.0162624
  4. Barrientos, J.A. & Sánchez-Corral, D. (2013) Nuevos datos sobre licósidos y agelénidos (Arachnida, Araneae) del Parque Natural de las Sierras de Cazorla, Segura y las Villas (Jaén, España). Revista Ibérica de Aracnología, 22, 21–35.
  5. Blagoev, G.A., deWaard, J.R., Ratnasingham, S., deWaard, S.L., Lu, L., Robertson, J. & Hebert, P.D.N. (2016) Untangling taxonomy: a DNA barcode reference library for Canadian spiders. Molecular Ecology Resources, 16, 325–341. https://doi.org/10.1111/1755-0998.12444
  6. Bolzern, A., Burckhardt, D. & Hänggi, A. (2013) Phylogeny and taxonomy of European funnel-web spiders of the Tegenaria-Malthonica complex (Araneae: Agelenidae) based upon morphological and molecular data. Zoological Journal of the Linnean Society, 168, 723–848. https://doi.org/10.1111/zoj.12040
  7. Bolzern, A. & Hänggi, A. (2016) Revision of the Nearctic Eratigena and Tegenaria species (Araneae: Agelenidae). Journal of Arachnology, 44 (2), 105–141. https://doi.org/10.1636/R15-81
  8. Bolzern, A. & Jäger, P. (2015) Unexpected occurrence of the genus Eratigena in Laos with description of a new species (Araneae: Agelenidae). Zootaxa, 3920 (3), 431–442. https://doi.org/10.11646/zootaxa.3920.3.2
  9. Bresjančeva, J. (1907) Prilog za poznavanje srpske aranejske faune. Travaux Faits au Laboratoire de Zoologie à l’Universitéde Belgrade, 1 (2–3), 1–16. [in Serbian]
  10. Briscoe, A.G., Goodacre, S., Masta, S.E., Taylor, M.I., Arnedo, M.A., Penney, D., Kenny, J. & Creer, S. (2013) Can long-range PCR be used to amplify genetically divergent mitochondrial genomes for comparative phylogenetics? A case study within spiders (Arthropoda: Araneae). PLoS ONE, 8 (5), e62404. https://doi.org/10.1371/journal.pone.0062404
  11. Coddington, J.A., Agnarsson, I., Cheng, R., Čandek, K., Driskell, A., Frick, H., Gregorič, M., Kostanjšek, R., Kropf, C., Kweskin, M., Lokovšek, T., Pipan, M., Vidergar, N. & Kuntner, M. (2016) DNA barcode data accurately assign higher spider taxa. PeerJ, 4, e2201. https://doi.org/10.7717/peerj.2201
  12. Croucher, P.J.P., Oxford, G.S. & Searle, J.B. (2004) Mitochondrial differentiation, introgression and phylogeny of species in the Tegenaria atrica group (Araneae: Agelenidae). Biological Journal of the Linnean Society, 81 (1), 79–89. https://doi.org/10.1111/j.1095-8312.2004.00280.x
  13. Deeleman-Reinhold, C.L. (1974) The cavespider fauna of Montenegro (Araneae). Glasnik Republickog Zavoda za Zastitu Prirode i Prirodnjackog Muzeja Titogradu, 6, 9–33.
  14. Deeleman-Reinhold, C.L. (1986) Contribution à la connaissance des Lepthyphantes du groupe pallidus (Araneae, Linyphiidae) de Yougoslavie, Grece et Chypre. Mémoires de Biospéologie, 12 (for 1985), 37–50.
  15. Domènech, M., Malumbres‐Olarte, J., Enguídanos, A., Múrria, C. & Arnedo, M.A. (2022) What DNA barcodes reveal: microhabitat preference, hunting strategy and dispersal ability drive genetic variation across Iberian spider species. Insect Conservation and Diversity, 15 (2), 248–262. https://doi.org/10.1111/icad.12552
  16. Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic acids research, 32 (5), 1792–1797. https://doi.org/10.1093/nar/gkh340
  17. Geci, D. & Naumova, M. (2021a) The Spotted Orb-weaver Neoscona byzanthina (Pavesi, 1876) - An Enigmatic but Common Species on the Balkans (Araneae: Araneidae). Ecologia Balkanica, Special Edition 4, 1–9.
  18. Geci, D. & Naumova, M. (2021b) A Preliminary Checklist of the Spiders of Kosovo (Arachnida: Araneae). Ecologia Balkanica, Special Edition 4, 11–28.
  19. Geci, D., Naumova, M., Ibrahimi, H., Grapci-Kotori, L., Gashi, A., Bilalli, A. & Musliu, M. (2023) A contribution to spider fauna (Arachnida: Araneae) from Bjeshkët e Nemuna mountains (Kosovo). Natura Croatica, 32 (2), 535–547. https://doi.org/10.20302/NC.2023.32.35
  20. Giachino, P.M. (2006) Bathyscidius comottiorum n. sp. del Kosovo (Coleoptera, Cholevidae, Leptodirinae). Rivista del Museo Civico di Storia Naturale Enrico Caffi, Bergamo, 24, 11–15. [2005]
  21. Grapci-Kotori, L., Geci, D., Naumova, M., Ibrahimi, H., Bilalli, A., Musliu, M., Kasmuaj, E. & Gashi, A. (2022) Spiders from Sharr mountain – new faunistic data (Arachnida: Araneae). Natura Croatica, 39 (2), 335–350. https://doi.org/10.20302/NC.2022.31.24
  22. Grimm, U. (1985) Die Gnaphosidae Mitteleuropas (Arachnida, Araneae). Abhandlungen des Naturwissenschaftlichen Vereins in Hamburg, Neue Folge, 26, 1–318.
  23. Grosser, C., Pešić, V., Berlajolli, V. & Gligorović, B. (2016) Glossiphonia balcanica n. sp. and Dina prokletijaca n. sp. (Hirudinida: Glossiphoniidae, Erpobdellidae) - two new leeches from Montenegro and Kosovo. Ecologica Montenegrina, 8, 17–26. https://doi.org/10.37828/em.2016.8.2
  24. Gullefors, B. (2002) Sveriges nattsländor (Trichoptera), en provinskatalog med nyare fynduppgifter. Entomologisk Tidskrift, 123 (3), 131–147.
  25. Hoang, D.T., Chernomor, O., Von Haeseler, A., Minhc, B.Q. & Vinh, L.S. (2018) UFBoot2: improving the ultrafast bootstrap approximation. Molecular Biology and Evolution, 35 (2), 518–522. https://doi.org/10.1093/molbev/msx281
  26. Ibrahimi, H., Bilalli, A., Vitecek, S., Pauls, S.U., Erzinger, F., Gashi, A., Grapci-Kotori, L., Geci, D., Musliu, M. & Kasumaj, E. (2021) Potamophylax coronavirus sp. n. (Trichoptera: Limnephilidae), a new species from Bjeshkët e Nemuna National Park in the Republic of Kosovo, with molecular and ecological notes. Biodiversity Data Journal, 9, e64486. https://doi.org/10.3897/BDJ.9.e64486
  27. Ibrahimi, H., Hlebec, D., Bilalli, A., Musliu, M., Previšić, A., Gashi, A., Grapci-Kotori, L., Cerjanec, D., Geci, D. & Kučinić, M. (2021) Rhyacophila siparantum sp. nov. (Trichoptera: Rhyacophilidae), a new species of the R. philopotamoides species group from the Republic of Kosovo with molecular and ecological notes. Ecologica Montenegrina, 49, 12–22. https://doi.org/10.37828/em.2021.49.1
  28. Ibrahimi, K., Islami, B., Kastrati, B., Geci, D., Bilalli, A. & Ibrahimi, H. (2024) New findings and an updated checklist of Araneidae (Arachnida: Araneae) from Kosovo. Journal of Insect Biodiversity and Systematics, 10, 547–555. https://doi.org/10.61186/jibs.10.3.547
  29. Ibrahimi, H., Kučinić, M., Vitecek, S., Waringer, J., Graf, W., Previšić, A., Bálint, M., Keresztes, L. & Pauls, S.U. (2015) New records for the Kosovo caddisfly fauna with the description of a new species, Drusus dardanicus sp. nov. (Trichoptera: Limnephilidae). Zootaxa, 20, 4032 (5), 551–68. https://doi.org/10.11646/zootaxa.4032.5.5
  30. Ibrahimi, H., Vitecek, S., Previšićk, A., Kučinić, M., Waringer, J., Graf, W., Balint, M., Keresztes, L. & Pauls, S.U. (2016) Drusus sharrensis sp. n. (Trichoptera, Limnephilidae), a new species from Sharr National Park in Kosovo, with molecular and ecological notes. ZooKeys, 559, 107–124. https://doi.org/10.3897/zookeys.559.6350
  31. Islami, B., Ibrahimi, K., Ibrahimi, H., Grapci-Kotori, L., Bilalli, A., Musliu, M., Gashi, A & Geci, D. (2024) Fauna of Kosovo. Version 1. Available from: https://www.faunaofkosovo.info (accessed 31 March 2025)
  32. Johnson, M., Zaretskaya, I., Raytselis, Y., Merezhuk, Y., McGinnis, S. & Madden, T.L. (2008) NCBI BLAST: a better web interface. Nucleic Acids Research, 1, 36. https://doi.org/10.1093/nar/gkn201
  33. Knoflach, B. (1996) Die Arten der Steatoda phalerata -Gruppe in Europa (Arachnida: Araneae, Theridiidae). Mitteilungen der Schweizerischen Entomologischen Gesellschaft, 69 (3–4), 377–404. https://doi.org/10.5169/seals-402640
  34. Koch, C.L. (1837) Übersicht des Arachnidensystems. Heft 1. C.H. Zeh’sche Buchhandlung, Nürnberg, 39 pp. https://doi.org/10.5962/bhl.title.39561
  35. Kolosváry, G. (1938) Sulla Fauna Arachnologica della Jugoslavia. Rassegna Faunistica, 16 (3–4), 3–23.
  36. Kolosváry, G. (1940) Explorationes zoologicae ab E. Csiki in Albania peractae. A Magyar Tudományos Akadémia Balkán–KutatásainakTudományos Eredményei. Budapest, I (XIX), 330–332.
  37. Kontschán, J. (2006) Some zerconid mites (Acari: Mesostigmata: Zerconidae) from Kosovo (Serbia–Montenegro) with description of Zercon kosovina sp. nov. Zootaxa, 1276 (1), 49–5. https://doi.org/10.11646/zootaxa.1276.1.4
  38. Kratochvíl, J. (1935) Araignées nouvelles ou non encore signalées en Yougoslavie. Première partie. Folia Zoologica et Hydrobiologica, Rigā, 8, 10–25.
  39. Kryštufek, B. & Reed, J. (2004) Pattern and Process in Balkan Biodiversity: An Overview. In: Griffiths, H.I., Kryštufek, B. & Reed, J.M. (Eds.), Balkan Biodiversity: Pattern and Process in the European Hotspot. Springer, Dordrecht, pp. 1–8. https://doi.org/10.1007/978-1-4020-2854-0_1
  40. Kuralt, Ž., Ratajc, U., Arambašić, N., Ferle, M., Gabor, M. & Kos, I. (2022) Inventory and DNA-barcode library of ground-dwelling predatory arthropods from Krokar virgin forest, Slovenia. Biodiversity Data Journal, 10, e77661. https://doi.org/10.3897/BDJ.10.e77661
  41. Lecigne, S., Moutaouakil, S. & Lips, J. (2023) Contribution to the knowledge of the spider fauna of Morocco (Arachnida: Araneae)—first note—on new records of cave spiders. Arachnologische Mitteilungen, 66, 44–71. https://doi.org/10.30963/aramit6607
  42. Macías-Hernández, N., Domènech, M., Cardoso, P., Emerson, B.C., Borges, P.A.V., Lozano-Fernandez, J., Paulo, O.S., Vieira, A., Enguídanos, A., Rigal, F., Amorim, I.R. & Arnedo, M.A. (2020) Building a robust, densely-sampled spider tree of life for ecosystem research. Diversity, 12 (8), 288. https://doi.org/10.3390/d12080288
  43. Maxhuni, Q., Morina, I., Morina, R., Ferizi, R. & Hashani, Z. (2014) Biodiversity and protected areas in Kosovo. International Journal of Ecosystems and Ecology Sciences, 4 (1), 173–178.
  44. Minh, B.Q., Schmidt, H.A., Chernomor, O., Schrempf, D., Woodhams, M.D., von Haeseler, A. & Lanfear, R. (2020) IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era. Molecular Biology and Evolution, 37 (5), 1530–1534. https://doi.org/10.1093/molbev/msaa015
  45. Nentwig, W., Blick, T., Bosmans, R., Gloor, D., Hänggi, A. & Kropf, C. (2025) Spiders of Europe. Version 3.2025. https://doi.org/10.24436/1
  46. Oxford, G. & Bolzern, A. (2018) Molecules v. morphology—is Eratigena atrica (Araneae: Agelenidae) one species or three? Arachnology, 17 (7), 337–357. https://doi.org/10.13156/arac.2017.17.7.337
  47. Perreau, M. (2008) Pavicevicia n. gen., a new genus with one new species of Bathyciotina from Kosovo (Coleoptera, Leiodidae, Cholevinae, Leptodirini). Advances in the Studies of the Fauna of the Balkan Peninsula, 22, 177–182.
  48. Pfingstl, T., Lienhard, A., Shimano, S., Yasin, Z.B., Shau-Hwai, A.T., Jantarit, S. & Petcharad, B. (2019) Systematics, genetics, and biogeography of intertidal mites (Acari, Oribatida) from the Andaman Sea and Strait of Malacca. Journal of Zoological Systematics and Evolutionary Research, 57 (1), 91–112. https://doi.org/10.1111/jzs.12244
  49. Roslin, T., Somervuo, P., Pentinsaari, M., Hebert, P.D., Agda, J., Ahlroth, P. & Mutanen, M. (2022) A molecular-based identification resource for the arthropods of Finland. Molecular Ecology Resources, 22, 803–822. https://doi.org/10.1111/1755-0998.13510
  50. Schäffer, S., Krisper, G., Pfingstl, T. & Sturmbauer, C. (2008) Description of Scutovertex pileatus sp. nov. (Acari, Oribatida, Scutoverticidae) and molecular phylogenetic investigation of congeneric species in Austria. Zoologischer Anzeiger - A Journal of Comparative Zoology, 247 (4), 249–258. https://doi.org/10.1016/j.jcz.2008.02.001
  51. Sikes, D.S., Bowser, M., Morton, J.M., Bickford, C., Meierotto, S. & Hildebrandt, K. (2017) Building a DNA barcode library of Alaska’s non-marine arthropods. Genome, 60 (3), 248–259. https://doi.org/10.1139/gen-2015-0203
  52. Šilhavý, V. (1944) De Araneis familiae Thomisidae in Balcano occidentali viventibus. Sborník Klubu Přírodovědeckého v Brně, 25, 90–95.
  53. Stojićević, D. (1929) Les araignées de Serbie. Araneae Sund. Glasnevin Museum de Historia Natural, Beograd, 19, 1–65.
  54. Tamura, K., Stecher, G. & Kumar, S. (2021) MEGA11: molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution, 38 (7), 3022–3027. https://doi.org/10.1093/molbev/msab120
  55. Vrenozi, B. & Jagaer, P. (2013) Spiders (Araneae) from Albania and Kosovo in the collection of Carl Friedrich Roewer. Arachnologische Mitteilungen, 46, 17–26. https://doi.org/10.5431/aramit4604
  56. Wheeler, W.C., Coddington, J.A., Crowley, L.M., Dimitrov, D., Goloboff, P.A., Griswold, C.E., Hormiga, G., Prendini, L., Ramírez, M.J., Sierwald, P., Almeida-Silva, L., Alvarez-Padilla, F., Arnedo, M.A., Benavides-Silva, L.R., Benjamin, S.P., Bond, J.E., Grismado, C.J., Hasan, E., Hedin, M., Izquierdo, M.A., Labarque, F.M., Ledford, J., Lopardo, L., Maddison, W.P., Miller, J.A., Piacentini, L.N., Platnick, N.I., Polotow, D., Silva-Dávila, D., Scharff, N., Szűts, T., Ubick, D., Vink, C.J., Wood, H.M. & Zhang, J. (2017) The spider tree of life: phylogeny of Araneae based on target‐gene analyses from an extensive taxon sampling. Cladistics, 33 (6), 574–616. https://doi.org/10.1111/cla.12182
  57. World Spider Catalog. (2025) World Spider Catalog. Version 26. Natural History Museum Bern, Bern. Available from: http://wsc.nmbe.ch (accessed 31 March 2025) https://doi.org/10.24436/2
  58. Wunderlich, J. (1984) Beschreibung der Wolfsspinne Pardosa pseudolugubris n. sp. und Revision der Pardosa amentata- Gruppe, zugleich ein Beitrag zur Kenntnis der innerartlichen Variabilität bei Spinnen (Arachnida: Araneae: Lycosidae). Neue Entomologische Nachrichten, 10, 1–15.
  59. Zhao, Z. & Li, S. (2017) Extinction vs. rapid radiation: the juxtaposed evolutionary histories of coelotine spiders support the Eocene–Oligocene orogenesis of the Tibetan Plateau. Systematic Biology, 66 (6), 988–1006. https://doi.org/10.1093/sysbio/syx042

How to Cite

Geci, D., Ibrahimi, H., Naumova, M., Bilalli, A., Koblmüller, S., Musliu, M., Grapci-Kotori, L., Gashi, A., Schäffer, S. & Wagner, M. (2025) Description of a new Eratigena Bolzern, Burckhardt & Hänggi, 2013 (Arachnida: Araneae: Agelenidae) from the Balkans based on an integrative approach. Zootaxa, 5636 (1), 144–162. https://doi.org/10.11646/zootaxa.5636.1.6