Abstract
Hypotelus Erichson is a genus of minute rove beetles widely distributed in Central and South America. The species were recently revised and the monophyly of the genus was confirmed based on morphological data. We describe a new species from Ecuador, provide scanning electron microscope images to demonstrate the diagnostic characteristics of the genus and include the new species in a dichotomous key. We also reassess the monophyly of the genus, now including the new species, using morphological and molecular data combined. Hypotelus chusqueaticus sp. nov. is similar to H. testaceus, but differs by having elytra unicolored, male sternite 8 with posterior margin somewhat pointed and female sternite 8 with posterior margin projected in middle-third. Including the new species, Hypotelus is confirmed as a monophyletic group with three internal clades. The new species is closely related to H. marginatus.
References
- Abouheif, E. & Wray, G.A. (2002) Evolution of the gene network underlying wing polyphenism in ants. Science, 297, 249–252. https://doi.org/10.1126/science.1071468
- Arriaga-Varela, E., Tomaszewska, W., Szawaryn, K., Robertson, J., Seidel, M., Ślipiński, A. & Fikáček, M. (2023) The resurrection of Cerasommatidiidae, an enigmatic group of coccinelloid beetles (Coleoptera: Coccinelloidea) based on molecular and morphological evidence. Zoological Journal of the Linnean Society, 197, 1078–1115. https://doi.org/10.1093/zoolinnean/zlac082
- Asghar, U., Malik, M.F., Anwar, F., Javed, A. & Raza, A. (2015) DNA extraction from insects by using different techniques: a review. Advances in Entomology, 3, 132. https://doi.org/10.4236/ae.2015.34016
- Bortoluzzi, S., Caron, E. & Ribeiro-Costa, C.S. (2017) Revision and phylogeny of Hypotelus Erichson: a Neotropical genus of minute rove beetles (Coleoptera, Staphylinidae, Piestinae). Zootaxa, 4273 (4), 451–487. https://doi.org/10.11646/zootaxa.4273.4.1
- Caron, E., Ribeiro-Costa, C.S. & Newton, A.F. (2012) Cladistic analysis and revision of Piestus Gravenhorst, 12 S. Yamamoto et al. with remarks on related genera (Coleoptera: Staphylinidae: Piestinae). Invertebrate Systematics, 25, 490–585. https://doi.org/10.1071/IS10016
- Caron, E., Busanello, D.G.C., Buss, B.C. & Zilberman, B. (In press) Redefining Siagonium Kirby & Spence (Coleoptera, Staphylinidae, Piestinae): phylogeny, biogeography and taxonomy. Insect Systematics & Evolution.
- Cherman, M.A., Morón, M.A. & Almeida, L.M. (2016) Phylogenetic relationships within Diplotaxini Kirby (Coleoptera: Melolonthidae: Melolonthinae) with emphasis on Liogenys Guérin-Méneville. Systematic Entomology, 41, 744–770. https://doi.org/10.1111/syen.12188
- Dillon, N., Austin, A.D. & Bartowsky, E. (1996) Comparison of preservation techniques for DNA extraction from hymenopterous insects. Insect Molecular Biology, 5, 21–24.
- Goloboff, P.A., Farris, J.S., Källersjö, M., Oxelman, B., Ramírez, M.J. & Szumik, C.A. (2003) Improvements to resampling measures of group support. Cladistics, 19, 324–332. https://doi.org/10.1111/j.1096-0031.2003.tb00376.x
- Goloboff, P.A. & Catalano, S.A. (2016) TNT version 1.5, including a full implementation of phylogenetic morphometrics. Cladistics, 32, 221–238. https://doi.org/10.1111/cla.12160
- Goloboff, P.A., Torres, A. & Salvador Arias, J. (2018) Weighted parsimony outperforms other methods of phylogenetic inference under models appropriate for morphology. Cladistics, 34, 407–437. https://doi.org/10.1111/cla.12205
- Grebennikov, V.V. & Newton, A.F. (2012) Detecting the basal dichotomies in the monophylum of carrion and rove beetles (Insecta: Coleoptera: Silphidae and Staphylinidae) with emphasis on the Oxyteline group of subfamilies. Arthropod Systematics & Phylogeny, 70 (3), 133–165. https://doi.org/10.3897/asp.70.e31759
- Kuraku, S., Zmasek, C.M., Nishimura, O. & Katoh, K. (2013) aLeaves facilitates on-demand exploration of metazoan gene family trees on MAFFT sequence alignment server with enhanced interactivity. Nucleic Acids Research, 41, 22–28. https://doi.org/10.1093/nar/gkt389
- McKenna, D.D., Farrell, B.D., Caterino, M.S., Farnum, C.W., Hawks, D.C., Maddison, D.R., Seago, A.E., Short, A.E.Z., Newton, A.F. & Thayer, M.K. (2015) Phylogeny and evolution of Staphyliniformia and Scarabaeiformia: Forest litter as a stepping stone for diversification of nonphytophagous beetles. Systematic Entomology, 40, 35–60. https://doi.org/10.1111/syen.12093
- Minh, B.Q., Nguyen, M.A.T. & von Haeseler, A. (2013) Ultrafast approximation for phylogenetic bootstrap. Molecular Biology and Evolution, 30, 1188–1195. https://doi.org/10.1093/molbev/mst024
- Minh, B.Q., Schmidt, H.A., Chernomor, O., Schrempf, D., Woodhams, M.D., Von Haeseler, A. & Lanfear, R. (2020) IQ-TREE 2: new models and efficient methods for phylogenetic inference in the genomic era. Molecular Biology and Evolution, 37, 1530–1534. https://doi.org/10.1093/molbev/msaa015
- Nixon, K.C. (2002) WinClada. Version 1.00.08. Published by the author, Ithaca, NY. [software]
- Orlov, I., Leschen, R.A., Żyła, D. & Solodovnikov, A. (2021) Total evidence backbone phylogeny of Aleocharinae (Coleoptera: Staphylinidae). Cladistics, 37, 343–374.
- Page, R. (2001) NDE (NEXUS Data Editor for Windows). Version 0.5.0. Published by the author, Glasgow. [software]
- Robertson, J.A. & Moore, W. (2017) Phylogeny of Paussus L. (Carabidae: Paussinae): unravelling morphological convergence associated with myrmecophilous life histories. Systematic Entomology, 42, 134–170. https://doi.org/10.1111/syen.12205
- Simón, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America, 87, 651–701. https://doi.org/10.1093/aesa/87.6.651
- Thayer, M.K. (2016) Staphylinidae Latreille, 1802. In: Beutel, R.G. & Leschen, R.A.B. (Eds.), Handbook of Zoology. Arthropoda: Insecta, Coleoptera, Beetles. Volume 1: Morphology and systematics (Archostemata, Adephaga, Myxophaga, Polyphaga partim). De Gruyter, Berlin and Boston, pp. 394–442.
- Tamura, K., Stecher, G. & Kumar, S. (2021) MEGA 11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution, 4, 21–28. https://doi.org/10.1093/molbev/msab120
- Tian, E.W. & Yu, H. (2013) Simple and rapid DNA extraction protocol of small insects for PCR amplification. Entomological News, 123, 303–310. https://doi.org/10.3157/021.123.0403
- Wang, Q. & Wang, X. (2012) Comparison of methods for DNA extraction from a single chironomid for PCR analysis. Pakistan Journal of Zoology, 44, 421–426.
- Ward, P.S. & Downie, D.A. (2005) The ant subfamily Pseudomyrmecinae (Hymenoptera: Formicidae): phylogeny and evolution of big‐eyed arboreal ants. Systematic Entomology, 30, 310–335. https://doi.org/10.1111/j.1365-3113.2004.00281.x
- Yamamoto, S., Caron, E. & Bortoluzzi, S. (2018) Propiestus archaicus, the first Mesozoic amber inclusion of piestine rove beetles and its evolutionary and biogeographical significance (Coleoptera: Staphylinidae: Piestinae). Journal of Systematic Palaeontology, 17, 1257–1270. https://doi.org/10.1080/14772019.2018.1517282
- Zhang, D., Gao, F., Jakovlić, I., Zou, H., Zhang, J., Li, W.X. & Wang, G.T. (2020) PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources, 20, 348–355. https://doi.org/10.1111/1755-0998.13096