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Type: Article
Published: 2023-06-01
Page range: 282-290
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The identity of Tokays (Squamata: Gekkonidae: Gekko) from Hong Kong

Freshwater Ecology and Conservation Laboratory; School of Biological Sciences; The University of Hong Kong; Pokfulam; Hong Kong SAR; China
Freshwater Ecology and Conservation Laboratory; School of Biological Sciences; The University of Hong Kong; Pokfulam; Hong Kong SAR; China
Department of Natural Resources Science; University of Rhode Island; Kingston; Rhode Island 02881 USA
Reptilia Gekko reevesii Gekko gecko translocation matrilineal genealogy

Abstract

The Tokay gecko (Gekko gecko), found throughout Southeast Asia and India, is a heavily harvested species of high commercial value. Recent studies have supported the elevation of the black morph of Tokay gecko, found only in southern China and northern Vietnam, to the species Gekko reevesii. Previous genetic studies focused on specimens of G. reevesii from southwestern populations. Hong Kong, in southeastern China, has native populations of G. reevesii. To verify the identity of G. reevesii in Hong Kong, we employed three mitochondrial genes (COI, Cytb & ND2) and constructed a matrilineal genealogy using other specimens from Guangxi (southwestern China) and northern Vietnam, as well as G. gecko from a wide range of Southeast Asian countries. Our study confirmed that G. reevesii occurs naturally in Hong Kong, but one exotic population of G. gecko, likely a translocation from international trade, was also revealed. Our study did not reject the species ranking of G. reevesii. Moreover, like previous studies, we recovered a paraphyletic G. gecko, which may reflect a species complex, hybridization or incomplete lineage sorting. More extensive sampling of the two species over a broader range of their asserted distribution together with the use of both mitochondrial and nuclear DNA are required to better investigate their biogeography.

 

References

  1. Abadi, S., Azouri, D., Pupko, T. & Mayrose, I. (2019) Model selection may not be a mandatory step for phylogeny reconstruction. Nature Communications, 10 (1), 934. https://doi.org/10.1038/s41467-019-08822-w
  2. Agarwal, I., Ceríaco, L.M.P., Metallinou, M., Jackman, T.R. & Bauer, A.M. (2021) How the African house gecko (Hemidactylus mabouia) conquered the world. Royal Society Open Science, 8 (210749), 1–14. https://doi.org/10.1098/rsos.210749
  3. Aowphol, A., Yodthong, S., Rujirawan, A. & Thirakhupt, K. (2019) Mitochondrial diversity and phylogeographic patterns of Gekko gecko (Squamata: Gekkonidae) in Thailand. Asian Herpetological Research, 10 (3), 158–169.
  4. Bauer, A.M. (2009) Geckos in traditional medicine: Forensic implications. Applied Herpetology, 6 (1), 81–96. https://doi.org/10.1163/157075408X397509
  5. Caillabet, O.S. (2013) The Trade in Tokay Geckos Gekko gecko in South-east Asia: with a Case Study on Novel Medical Claims in Peninsular Malaysia. TRAFFIC, Petaling Jaya, Selangor, 34 pp.
  6. Chan, S.W. (2006) Religious release of birds in Hong Kong. Master of Philosophy thesis, the University of Hong Kong, Hong Kong, 263 pp.
  7. Chan, S.K.F., Cheung, K., Ho, C., Lam, F. & Tang, W. (2006) The geckos of Hong Kong. Hong Kong Biodiversity, 13, 1–9.
  8. Convention on International Trade in Endangered Species of Wild Fauna and Flora [CITES] (2019) CITES Appendices. Available from: https://www.cites.org/eng/app/appendices.php (accessed 5 August 2020)
  9. Damas-Moreira, I., Riley, J.L., Carretero, M.A., Harris, D.J. & Whiting, M.J. (2020) Getting ahead: exploitative competition by an invasive lizard. Behavioral Ecology and Sociobiology, 74, 117. https://doi.org/10.1007/s00265-020-02893-2
  10. Downes, S. & Bauwens, D. (2002) An experimental demonstration of direct behavioural interference in two Mediterranean lacertid lizard species. Animal Behaviour, 63, 1037–1046. https://doi.org/10.1006/anbe. 2002.3022
  11. Ficetola, G.F., Silva‐Rocha, I.R., Carretero, M.A., Vignoli, L., Sacchi, R., Melotto, A., Scali, S. & Salvi, D. (2021) Status of the largest extant population of the critically endangered Aeolian lizard Podarcis raffonei (Capo Grosso, Vulcano Island). PLoS ONE, 16 (6), e0253631. https://doi.org/10.1371/journal.pone.0253631.t001
  12. Gray, J.E. (1831) A synopsis of the species of the class Reptilia. In: Griffith, E. & Pidgeon, E. (Eds.), The Animal Kingdom Arranged in Conformity with its Organization, by the Baron Cuvier. Vol. 9. The Class Reptilia Arranged by the Baron Cuvier, with Specific Descriptions. Whittaker, Treacher & Co, London, pp. 1–110.
  13. Harris, D.J. (2002) Reassessment of comparative genetic distance in reptiles from the mitochondrial cytochrome b gene. Journal of Herpetology, 12, 85–86.
  14. Henderson, R.W., De Latte, A. & McCarthy, T.J. (1993) Gekko gecko (Sauria: Gekkonidae) established on Martinique, French West Indies. Caribbean Journal of Science, 29 (1–2), 128–129.
  15. Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogeny. Bioinformatics, 17 (8), 754–755. https://doi.org/10.1093/bioinformatics/17.8.754
  16. Jančúchová-Lásková, J., Landová, E. & Frynta, D. (2015) Experimental crossing of two distinct species of leopard geckos, Eublepharis angramainyu and E. macularius: viability, fertility and phenotypic variation of the hybrids. PLoS ONE, 10 (12), e0143630.
  17. Kowalczyk, M., Staniszewski, A., Kamiñska, K., Domaradzki, P. & Horecka, B. (2021) Advantages, possibilities, and limitations of mitochondrial DNA analysis in molecular identification. Folia Biologica, Kraków, 69 (3), 101–111. https://doi.org/10.3409/fb_69-3.12
  18. Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35 (6), 1547–1549. https://doi.org/10.1093/molbev/msy096
  19. Lau, M.W.N., Ades, G., Goodyer, N. & Zou, F. (1997) Wildlife trade in southern China including Macau and Hong Kong. In: Mackinnon, J. & Sung, W. (Eds.), Conserving China’s Biodiversity. China Environmental Science Press, Beijing, pp. 141–159.
  20. Lee, K., Chen, T., Shang, G., Clulow, S., Yang, Y. & Lin, S. (2019) A check list and population trends of invasive amphibians and reptiles in Taiwan. ZooKeys, 829, 85–130. https://doi.org/10.3897/zookeys.829.27535
  21. Lever, C. (2003) Naturalized Reptiles and Amphibians of the World. Oxford University Press, New York, New York, 344 pp.
  22. Macey, J.R., Larson, A., Ananjeva, N.B., Fang, Z. & Papenfuss, T.J. (1997) Two novel gene orders and the role of lightstrand replication in rearrangement of the vertebrate mitochondrial genome. Molecular Biology and Evolution, 14, 91–104.
  23. Marshall, T.L., Chambers, E.A., Matz, M.V. & Hillis, D.M. (2021) How mitonuclear discordance and geographic variation have confounded species boundaries in a widely studied snake. Molecular Phylogenetics and Evolution, 162 (1–3), 107194.
  24. Meshaka, W.E., Clouse, R.M. & McMahon, L. (1997) Diet of the tokay gecko (Gekko gecko) in Southern Florida. Florida Field Naturalist, 25 (3), 105–107.
  25. Nguyen, T.Q., Cai, B. & Yang, J. (2021) Gekko reevesii. The IUCN Red List of Threatened Species, 2021, e.T104717831A104718941. Available from: https://doi.org/10.2305/IUCN.UK.2021-2.RLTS.T104717831A104718941.en (accessed 4 February 2022)
  26. Peng, Q.K., Wang, G.C., Yang, D., Yue, B.S., Li, L. & Zou, F.D. (2010) Genetic variability of the tokay gecko based on microsatellite analysis. Biochemical Systematics and Ecology, 38, 23–28. https://doi.org/10.1016/j.bse.2009.12.021
  27. Posada, D. (2008). jModelTest: phylogenetic model averaging. Molecular Biology and Evolution, 25 (7), 1253–1256. https://doi.org/10.1093/molbev/msn083
  28. Qin X.M., Li H.M., Zeng Z.H., Zeng D.L. & Guan, Q.X. (2012) Genetic variation and differentiation of Gekko gecko from different populations based on mitochondrial cytochrome b gene sequences and karyotypes. Zoological Science, 29 (6), 384–389. https://doi.org/10.2108/zsj.29.384
  29. Qin, X.M., Liang, Y.N. & Huang, X.Y. (2006) Isozymes analysis on different tissues from three different populations of Gekko gecko. Guangxi Sciences. 13, 310–315. [in Chinese]
  30. Qin, X.M., Qian, F. & Zeng, Z.H. (2009) Sequence variation and differentiation of mitochondrial cytochrome b gene of red Gekko gecko and black Gekko gecko [in Chinese]. Journal of Anhui Agriculture, 37, 6383–6384.
  31. Qin, X.M., Zeng, Z.H. & Liang, Y.N. (2007) Genetic variation and differentiation of gekko gecko from different populations [in Chinese]. Zoological Research, 28, 286–290.
  32. Rocha J.C. (2015) Occurrence of the Tokay Gecko, Gekko gecko (Linnaeus 1758) (Squamata, Gekkonidae), an exotic species in southern Brazil. Herpetology Notes, 8, 8–10.
  33. Rösler, H., Bauer, A.M., Heinicke, M.P., Greenbaum, F., Jackman, T., Nguyen, N.Q. & Ziegler, T. (2011) Phylogeny, taxonomy, and zoogeography of the genus Gekko Laurenti, 1768 with the revalidation of G. reevesii Gray, 1831 (Sauria: Gekkonidae). Zootaxa, 1989 (1), 1–50. https://doi.org/10.11646/zootaxa.2989.1.1
  34. Saijuntha, W., Sedlak, S., Agatsuma, T., Jongsomchai, K., Pilap, W., Kongbuntad, W., Tawong, W., Suksavate, W., Petney, T.N. & Tantrawatpan, C. (2019) Genetic structure of the red-spotted tokay gecko, Gekko gecko Linnaeus, 1758 (Squamata: Gekkonidae) from Mainland Southeast Asia. Asian Herpetological Research, 10 (2), 69–78. https://doi.org/10.16373/j.cnki.ahr.180066
  35. Toews, D.P.L. & Brelsford, A. (2012) The biogeography of mitochondrial and nuclear discordance in 756 animals. Molecular Ecology, 21, 3907–3930.
  36. Wang, G., Gong, S., Jiang, L., Peng, R., Shan, X., Zou, D., Yang, C. & Zou, F. (2013) Genetic variability of the Tokay gecko based on mitochondrial and nuclear DNA. Mitochondrial DNA, 24 (5), 518–527. https://doi.org/10.3109/19401736.2013.770488
  37. Wang, G., Peng, Q., Wu, L., Wang, T., Peng, R., Li, L., Zeng, Z. & Zou, F. (2012) Nuclear and mitochondrial DNA reveals significant intraspecific genetic differentiation of tokay gecko in southern China and northern Vietnam. Journal of Zoology, London, 287, 215–223. https://doi.org/10.1111/j.1469-7998.2012.00906.x
  38. Wei, S.L., Zeng, Z.H., Li, C.F. & Qin, X.M. (2008) Analysis of the Cyt b gene sequence in five Gekkonidae animals. Journal of Anhui Agricultural Sciences, 36 (6), 2268–2269. [in Chinese]
  39. Yang, D.T. & Rao, D.Q. (2008). Amphibia and Reptilia of Yunnan. Yunnan Science and Technology Press, Kunming, 411 pp.
  40. Yu, X., Peng, Y., Aowphol, A., Ding, L., Brauth, S.E. & Tang, Y.Z. (2011) Geographic variation in the advertisement calls of Gekko gecko in relation to variations in morphological features: implications for regional population differentiation. Ethology Ecology & Evolution, 23 (3), 211– 228. https://doi.org/10.1080/03949370.2011.566581
  41. Yuan, J.Q. & L. Li. (2008) Herbal textual of black-spot and red-spot G. gecko [in Chinese]. Journal of Chinese Medicinal Materials, 31, 1437–1439.
  42. Zardoya, R. & Meyer, A. (1996) Phylogenetic performance of mitochondrial protein-coding genes in resolving relationships among vertebrates. Molecular Biology and Evolution, 13 (7), 933– 942. https://doi.org/10.1093/oxfordjournals.molbev.a025661
  43. Zhang, Y., Chen, C., Li, L., Zhao, C., Chen, W. & Huang, Y. (2014) Insights from ecological niche modelling on the taxonomic distinction and niche differentiation between the black-spotted and red-spotted tokay geckoes (Gekko gecko). Ecology and Evolution, 4 (17), 3383–3394. https://doi.org/10.1002/ece3.1183
  44. Zhang, Y.Y., Mo, X.C., Zeng, W.M. & Hu, D.N. (2006) A molecular phylogeny of Red G. gecko and Black G. gecko (Gekko gecko) based on mitochondrial 12S rRNA gene sequences [in Chinese]. Guangxi Medical Journal, 28, 793–796.
  45. Zhang, Q.Q., Tang, Y.Z., Huang, Y.C. & Zeng. F.H. (1997) Investigation on the geographic variance of tokay, Gekko gecko L. Chinese Journal of Zoology, 32, 44–46. [in Chinese]