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Type: Article
Published: 2023-05-22
Page range: 333-348
Abstract views: 964
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A new species of Andean frog of the genus Phrynopus (Anura: Strabomantidae) from southeastern Peru

Asociación Pro Fauna Silvestre; Museo de Historia Natural de la Universidad Nacional San Cristóbal de Huamanga
Universidad Señor de Sipán
Museo de Historia Natural de la Universidad Nacional de San Antonio Abad del Cusco; Department of Biological Sciences; Florida International University; Centro de Ornitología y Biodiversidad; Instituto Peruano de Herpetología
Amphibia Andes Apurímac River Ayacucho humid puna VRAEM


The Cordillera de los Andes is one of the most important regions for biodiversity. Among amphibians, many endemic species of terrestrial-breeding frogs have recently been discovered. Herein we describe Phrynopus sancristobali from the Andes of southeastern Peru based on molecular and morphological data. The new species is known from the ecotone between humid puna and montane forest at 3910 m a.s.l. on the left side of the Apurímac Valley in the Department of Ayacucho. The new species differs from congeners by having dorsum bearing pustules and light brown with dark brown reticulations surrounding the areolas, and coloration consisting of flanks golden brown with gray or dark brown marks, palms pale orange, soles deep orange, toes IV and V dark brown in dorsal and ventral view, belly cream to yellow with gray to light brown marks, and groin and throat deep orange. The snout-vent length (SVL) is 20.7 and 22.2 in two females, and 19.5 mm in one male. According to our phylogeny inferred using Maximum Likelihood with a concatenated dataset of three mitochondrial and two nuclear genes, P. sancristobali is sister taxon of P. apumantarum, recently described from Department Huancavelica. Our description extends the known geographic range of Phrynopus 73 km to the south, and P. sancristobali is the only species in the genus known to occur south of the Mantaro River, whose deep valley is hypothesized to be a biogeographic barrier for high-Andean organisms. The discovery of P. sancristobali confirms the high levels of endemism and beta diversity of Phrynopus in the moist puna grasslands and montane forests of the high Andes of Peru, and suggests that further work will reveal the presence of additional species in southern Peru.



  1. Cannatella, D.C. (1984) Two new species of the leptodactylid frog genus Phrynopus, with comments on the phylogeny of the genus. Occasional Papers of the Museum of Natural History, University of Kansas, 113, 1–16.
  2. Catenazzi, A. (2015) State of the world’s amphibians. Annual Review of Environment and Resources, 40, 91–119. DOI:
  3. Catenazzi, A. & Lehr, E. (2018) Pristimantis antisuyu sp. n. and Pristimantis erythroinguinis sp. n., two new species of terrestrial-breeding frogs (Anura, Strabomantidae) from the eastern slopes of the Andes in Manu National Park, Peru. Zootaxa, 4394 (2), 185–200. DOI:
  4. Catenazzi, A., Mamani, L., Lehr, E. & von May, R. (2020) A new genus of terrestrial-breeding frogs (Holoadeninae, Strabomantidae, Terrarana) from southern Peru. Diversity-Basel, 12, 184. DOI:
  5. Catenazzi, A. & Ttito, A. (2016) A new species of Psychrophrynella (Amphibia, Anura, Craugastoridae) from the humid montane forests of Cusco, eastern slopes of the Peruvian Andes. PeerJ, 4, e1807. DOI:
  6. Catenazzi, A. & von May, R. (2014) Conservation status of amphibians in Peru. Herpetological Monographs, 28, 1–23. DOI:
  7. Chavez, G., Ayachi, L.A.G. & Catenazzi, A. (2020) A new species of frog (Terrarana, Strabomantidae, Phrynopus) from the Peruvian Andean grasslands. PeerJ, 8, e9433. DOI:
  8. Chávez, G., Garcia-Ayachi, L.A. & Catenazzi, A. (2023) A new species of frog (Terrarana, Strabomantidae, Phrynopus) from the Peruvian Andean grasslands. Evolutionary Systematics, 7, 105–116. DOI:
  9. De La Riva, I. (2020) Unexpected deta-diversity radiations in highland clades of Andean Terraranae frogs. In: Rull, V. & Carnaval, A.C. (Eds.), Neotropical Diversification: Patterns and Processes, Fascinating Life Sciences. Springer, Cham, pp. 741–764. DOI:
  10. De la Riva, I., Chaparro, J.C., Castroviejo-Fisher, S. & Padial, J.M. (2018) Underestimated anuran radiations in the high Andes: five new species and a new genus of Holoadeninae, and their phylogenetic relationships (Anura: Craugastoridae). Zoological Journal of the Linnean Society, 182, 129–172. DOI:
  11. Duellman, W.E. (2000) Leptodactylid frogs of the genus Phrynopus in northern Peru with descriptions of three new species. Herpetologica, 56, 273–285.
  12. Duellman, W.E. & Lehr, E. (2009) Terrestrial-breeding frogs (Strabomantidae) in Peru. Natur und Tier-Verlag, Münster, 384 pp.
  13. Fouquet, A., Gilles, A., Vences, M., Marty, C., Blanc, M. & Gemmell, N.J. (2007) Underestimation of species richness in Neotropical frogs revealed by mtDNA analyses. PLoS ONE, 2, e1109. DOI:
  14. Hedges, S.B., Duellman, W.E. & Heinicke, M.P. (2008) New World direct-developing frogs (Anura: Terrarana): molecular phylogeny, classification, biogeography, and conservation. Zootaxa, 1737 (1), 1–182. DOI:
  15. Katoh, K. & Standley, D. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution, 30, 772–780. DOI:
  16. Lajtman, T. & Mendoza, M. (2018) The use of the state of emergency as a repressive tool in contemporary Peru: The cases of the VRAEM and the Conga mining project. Revista Electronica de Estudios Latinoamericanos, 17, 1–19.
  17. Lehr, E. (2006) Taxonomic status of some species of Peruvian Phrynopus (Anura : Leptodactylidae), with the description of a new species from the Andes of Southern Peru. Herpetologica, 62, 331–347.[331:TSOSSO]2.0.CO;2 DOI:[331:TSOSSO]2.0.CO;2
  18. Lehr, E. (2007) New Eleutherodactyline frogs (Leptodactylidae: Pristimantis, Phrynopus) from Peru. Bulletin of the Museum of Comparative Zoology, 159, 145–178.[145:NEFLPP]2.0.CO;2 DOI:[145:NEFLPP]2.0.CO;2
  19. Lehr, E. & Catenazzi, A. (2008) A new species of Bryophryne (Anura : Strabomantidae) from southern Peru. Zootaxa, 1784 (1), 1–10. DOI:
  20. Lehr, E. & Catenazzi, A. (2009) Three new species of Bryophryne (Anura: Strabomantidae) from the Region of Cusco, Peru. South American Journal of Herpetology, 4, 125–138. DOI:
  21. Lehr, E. & Catenazzi, A. (2010) Two new species of Bryophryne (Anura: Strabomantidae) from high elevations in southern Peru (Region of Cusco). Herpetologica, 66, 308–319. DOI:
  22. Lehr, E., Fritzsch, G. & Muller, A. (2005a) Analysis of Andes frogs (Phrynopus, Leptodactylidae, Anura) phylogeny based on 12S and 16S mitochondrial rDNA sequences. Zoologica Scripta, 34, 593–603. DOI:
  23. Lehr, E., Lundberg, M. & Aguilar, U. (2005b) Three new species of Phrynopus from central Peru (Amphibia : Anura : Leptodactylidae). Copeia, 2005, 479–491. DOI:
  24. Lehr, E., Moravec, J. & Cusi, J.C. (2012) Two new species of Phrynopus (Anura, Strabomantidae) from high elevations in the Yanachaga-Chemillen National park in Peru (Departamento de Pasco). Zookeys, 235, 51–71. DOI:
  25. Lehr, E. & Oroz, A. (2012) Two new species of Phrynopus (Anura: Strabomantidae) from the Cordillera de Carpish in central Peru (Departamento de Huanuco). Zootaxa, 3512 (1), 53–63.
  26. Lehr, E. & Rodriguez, D. (2017) Two new species of Andes Frogs (Craugastoridae: Phrynopus) from the Cordillera de Carpish in central Peru. Salamandra, 53, 327–338. DOI:
  27. Lehr, E., von May, R., Moravec, J. & Cusi, J.C. (2017) A new species of Phrynopus (Amphibia, Anura, Craugastoridae) from upper montane forests and high Andean grasslands of the Pui Pui Protected Forest in central Peru. Zookeys, 713, 131–157. DOI:
  28. Linares-Palomino, R. (2006) Phytogeography and floristics of seasonally dry tropical forests in Peru. In: Pennington, R.T., Ratter, J.A. & Lewis, G.P. (Eds.), Neotropical Savannas and Seasonally Dry Forests: Plant Diversity, Biogeography and Conservation. CRC Press, Boca Raton, Florida, pp. 257–279. DOI:
  29. Lynch, J.D. (1975) A review of the Andean leptodactylid frog genus Phrynopus. Occasional Papers of the Museum of Natural History The University of Kansas, 35, 1–51.
  30. Lynch, J.D. & Duellman, W.E. (1997) Frogs of the genus Eleutherodactylus in western Ecuador. Systematics, ecology, and biogeography. . The University of Kansas Special Publication, 23, 1–236. DOI:
  31. Mamani, L. & Malqui, S. (2014) A new species of Phrynopus (Anura: Craugastoridae) from the central Peruvian Andes. Zootaxa, 3838 (2), 207–214. DOI:
  32. Nguyen, L.T., Schmidt, H.A., Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: A fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Molecular Biology and Evolution, 32 (1), 268–274. DOI:
  33. Pacheco, V., Salas, E., Barriga, C. & Rengifo, E. (2009) Diversidad de pequeños mamíferos en bosques montanos perturbados y no perturbados en el área de influencia del gasoducto de PERU LNG, cuenca del Río Apurímac, Ayacucho, Perú. In: Alonso, A., Dallmeier, F. & Servat, G. (Eds.), Monitoring biodiversity: Lessons from a Trans-Andean megaproject. Smithsonian Scholarly Press, Washington, D.C., pp. 305–316.
  34. Padial, J.M., Castroviejo-Fisher, S., Koehler, J., Vila, C., Chaparro, J.C. & De la Riva, I. (2009) Deciphering the products of evolution at the species level: the need for an integrative taxonomy. Zoologica Scripta, 38, 431–447. DOI:
  35. Rodriguez, L.O. & Catenazzi, A. (2017) Four new species of terrestrial-breeding frogs of the genus Phrynopus (Anura: Terrarana: Craugastoridae) from Rio Abiseo National Park, Peru. Zootaxa, 4273 (3), 381–406. DOI:
  36. Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599. DOI:
  37. Vences, M., Thomas, M., Bonett, R.M. & Vieites, D.R. (2005) Deciphering amphibian diversity through DNA barcoding: chances and challenges. Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 360, 1859–1868. DOI:
  38. Venegas, P.J., Barboza, A.C., De la Riva, I. & Padial, J.M. (2018) A new species of Phrynopus from the northeastern Andes of Peru, its phylogenetic position, and notes on the relationships of Holoadeninae (Anura: Craugastoridae). Zootaxa, 4446 (4), 501–524. DOI:
  39. Villena Del Carpio, D. & Annoni, D. (2016) El Valle de los Ríos Apurímac, Ene y Mantaro - Vraem: Entre el narcoterrorismo y el descaso del estado Peruano. Nuevo Derecho, 12, 107. DOI:
  40. von May, R., Lehr, E. & Rabosky, D.L. (2018) Evolutionary radiation of earless frogs in the Andes: molecular phylogenetics and habitat shifts in high-elevation terrestrial breeding frogs. Peerj, 6, e4313. DOI:
  41. Womack, M.C., Steigerwald, E., Blackburn, D., Cannatella, D.C., Catenazzi, A., Che, J., Koo, M., McGuire, J.A., Ron, S., Spencer, C., Vredenburg, V.T. & Tarvin, R.D. (2021) State of Amphibia 2020: Five years of amphibian research, diversity and resources. Ichthyology and Herpetology, 110, 638–661. DOI: