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Type: Article
Published: 2023-04-20
Page range: 207-230
Abstract views: 671
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Integrative taxonomy reveals two new narrowly-endemic crayfish species (Decapoda: Cambaridae) from the Yadkin River Basin in western North Carolina, USA

North Carolina Wildlife Resources Commission; 645 Fish Hatchery Road; Marion NC; 28752
North Carolina Museum of Natural Sciences; Research Laboratory; 1671 Gold Star Drive; Raleigh NC; 27669
North Carolina Wildlife Resources Commission; 645 Fish Hatchery Road; Marion NC; 28752
Crustacea biodiversity Blue Ridge Mountains Appalachian Mountains Cambarus robustus Cambarus species C species complex

Abstract

Two new species of stream-dwelling crayfish, Cambarus lapidosus, the Stony Fork Crayfish, and Cambarus burchfielae, the Falls Crayfish, are described from the Yadkin River basin in western North Carolina, USA, using an integrative taxonomic approach consisting of morphological, genetic, and biogeographic data. Both species were previously considered to be members of the widely distributed Cambarus species C complex, which occurs throughout mid-Atlantic Slope river basins; however, they are in fact morphologically and genetically more similar to the Cambarus robustus species complex from interior basins in the south-central Appalachians, indicating Atlantic basin stream capture of an Interior basin faunal group has occurred in this region. Both new species described herein can be differentiated from these two complexes, and each other, by several morphological characteristics. Cambarus lapidosus and C. burchfielae are differentiated from C. species C by the absence of cervical spines and the presence of 1–2 subpalmar tubercles on the chelae in most specimens; both species are less-punctate across the areola than C. aff. robustus. Cambarus burchfielae is further differentiated from C. lapidosus, C. species C, and C. aff. robustus by the presence of a narrower and sparsely punctate areola, a single weak row of tubercles on the mesial margin of the palm, and the absence of tubercles on the dorsal surface of the dactyl. The newly described species are genetically sister taxa and together are most similar by genetic distance to undescribed members of the C. robustus species complex from the nearby (~30 km) Watauga River basin and most phylogenetically similar to C. aff. robustus from the adjacent New River basin; both are interior basin drainages. The newly-described species are endemic to the Blue Ridge Mountains of western North Carolina and have restricted distributions (<100 km2–150 km2) in small (<4th order) tributaries to the Yadkin River. Cambarus lapidosus is known only from the upper Stony Fork watershed and C. burchfielae is known only from the upper reaches of the adjacent Lewis Fork watershed; both species likely qualify for conservation status protections under narrow geographic range criteria.

 

References

  1. Cooper, J.E. (2010) Annotated checklist of the crayfishes of North Carolina, and correlations of distributions with hydrologic units and physiographic provinces. Journal of the North Carolina Academy of Science, 126 (3), 69–76.
  2. Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics, and parallel computing. Nature Methods, 9, 772. https://doi.org/10.1038/nmeth.2109 DOI: https://doi.org/10.1038/nmeth.2109
  3. Folmer, O.F., Black, M.B., Hoeh, W.R. & Vrijenhoek, R.C. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3 (5), 294–299.
  4. Glon, M.G., Adams, S.B., Loughman, Z.J., Myers, G.A., Taylor, C.A. & Schuster, G.A. (2020) Two new species of burrowing crayfish in the genus Lacunicambarus (Decapoda: Cambaridae) from Alabama and Mississippi. Zootaxa, 4802 (3), 401–439. https://doi.org/10.11646/zootaxa.4802.3.1 DOI: https://doi.org/10.11646/zootaxa.4802.3.1
  5. Hobbs, H.H. Jr. (1981) The crayfishes of Georgia. Smithsonian Contributions to Zoology, 318, 1–549. https://doi.org/10.5479/si.00810282.318 DOI: https://doi.org/10.5479/si.00810282.318
  6. Hocutt, C.H., Jenkins, R.E. & Stauffer, J.R., Jr. (1986) Zoogeography of the fishes of the Central Appalachians and Central Atlantic Coastal Plain. In: Hocutt, C.H. & Wiley, E.O. (Ed.), The zoogeography of North American freshwater fishes. John Wiley and Sons, New York, New York, pp. 161–211.
  7. IUCN (2001) Categories and Criteria. Version 3.1. Available from: http://www.iucnredlist.org/static/categories_criteria_3_1 (accessed 9 November 2022)
  8. Jenkins, R.E. & Burkhead N.M. (1993) Freshwater Fishes of Virginia. American Fisheries Society, Bethesda, Maryland, 54–55.
  9. Kuchta, S.R., Haughey, M., Wynn, A.H., Jacobs, J.F. & Highton, R. (2016) Ancient river systems and phylogeographical structure in the spring salamander, Gyrinophilus porphyriticus. Journal of Biogeography, 43 (4), 639–652. https://doi.org/10.1111/jbi.12668 DOI: https://doi.org/10.1111/jbi.12668
  10. Kuhne, R.A. & Bailey, R.M. (1961) Stream capture and the distribution of the percid fish Etheostoma sagitta, with geologic and taxonomic considerations. Copeia, 1961, 1–8. https://doi.org/10.2307/1440163 DOI: https://doi.org/10.2307/1440163
  11. Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J. & Higgins, D.G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics, 23, 2947–2948. https://doi.org/10.1093/bioinformatics/btm404 DOI: https://doi.org/10.1093/bioinformatics/btm404
  12. Leray, M., Yang, J.Y., Meyer, C.P., Mills, S.C., Agudelo, N., Ranwez, V., Boehm, J.T. & Machida, R.J. (2013) A new versatile primer set targeting a short fragment of the mitochondrial COI region for metabarcoding metazoan diversity: application for characterizing coral reef fish gut contents. Frontiers in Zoology, 10, 34. https://doi.org/10.1186/1742-9994-10-34 DOI: https://doi.org/10.1186/1742-9994-10-34
  13. Loughman, Z.J. & Williams, B.W. (2021) Cambarus ectopistes sp. nov., a new stream-dwelling crayfish (Decapoda: Cambaridae) from the French Broad, Pigeon, and Nolichucky River watersheds in the Appalachian Mountain region of North Carolina and Tennessee, USA. Zootaxa, 5082 (4), 322–340. https://doi.org/10.11646/zootaxa.5082.4.2 DOI: https://doi.org/10.11646/zootaxa.5082.4.2
  14. Mayden, R.L. (1988) Vicariance biogeography, parsimony, and evolution in North America freshwater fishes. Systematic Zoology, 37, 329–355. https://doi.org/10.2307/2992197 DOI: https://doi.org/10.1093/sysbio/37.4.329
  15. NatureServe (2020) Element Rank Estimator version 3.2. Available from: https://www.natureserve.org/products/conservation-rank-calculator/download (accessed 9 November 2022)
  16. Perkins, M.A., Williams, B.W. & Russ, W.T. (2019) Cambarus franklini, a new crayfish (Decapoda: Cambaridae) from the Catawba River Basin in western North Carolina, USA. Zootaxa, 4568 (3), 520–532. https://doi.org/10.11646/zootaxa.4568.3.6 DOI: https://doi.org/10.11646/zootaxa.4568.3.6
  17. Prince, P.S., Spotila, J.A. & Henika, W.S. (2010) New physical evidence of the role of stream capture in active retreat of the Blue Ridge Escarpment, southern Appalachians. Geomorphology, 123, 305–319. https://doi.org/10.1016/j.geomorph.2010.07.023 DOI: https://doi.org/10.1016/j.geomorph.2010.07.023
  18. Prince, P.S., Spotila, J.A. & Henika, W.S. (2011) Stream capture as driver of transient landscape evolution in a tectonically quiescent setting. Geology, 39, 823–826. https://doi.org/10.1130/G32008.1 DOI: https://doi.org/10.1130/G32008.1
  19. Rambaut, A. (2018) FigTree. Version 1.4.4. Available from: http://tree.bio.ed.ac.uk/software/figtree/ (accessed 14 March 2023)
  20. Richman, N.I., Böhm, M., Adams, S.B., Alvarez, F., Bergey, E.A., Bunn, J.J.S., Burnham, Q., Cordeiro, J., Coughran, J., Crandall, K.A., Dawkins, K.L., DiStefano, R.J., Doran, N.E., Edsman, L., Eversole, A.G., Fureder, L., Furse, J.M., Gherardi, F., Hamr, P., Holdich, D.M., Horwitz, P., Johnston, K., Jones, C.M., Jones, J.P., Jones, R.L., Jones, T.G., Kawai, T., Lawler, S., López-Mejía, M., Miller, R.M., Pedraza- Lara, C., Reynolds, J.D., Richardson, A.M., Schultz, M.B., Schuster, G.A., Sibley, P.J., Souty-Grosset, C., Taylor, C.A., Thoma, R.F., Walls, J., Walsh, T.S. & Collen, B. (2015) Multiple drivers of decline in the global status of freshwater crayfish (Decapoda: Astacidea). Philosophical Transactions of the Royal Society of London B, 370, 20140060. https://doi.org/10.1098/rstb.2014.0060 DOI: https://doi.org/10.1098/rstb.2014.0060
  21. Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Hӧhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61 (3), 539–542. https://doi.org/10.1093/sysbio/sys029 DOI: https://doi.org/10.1093/sysbio/sys029
  22. Spotila, J.A., Bank, G.C., Reiners, P.W., Naeser, C.W., Naeser, N.D. & Henika, B.S. (2004) Origin of the Blue Ridge escarpment along the passive margin of Eastern North America. Basin Research, 16, 41–63. https://doi.org/10.1046/j.1365-2117.2003.00219.x DOI: https://doi.org/10.1111/j.1365-2117.2003.00219.x
  23. Tamura, K., Stecher, G. & Kumar, S. (2021) MEGA11: Molecular Evolutionary Genetics Analysis version 11. Molecular Biology and Evolution, 38, 3022–3027. https://doi.org/10.1093/molbev/msab120 DOI: https://doi.org/10.1093/molbev/msab120
  24. Taylor, C.A., Warren Jr., M.L., Fitzpatrick Jr., J.F., Hobbs III, H.H., Jezerinac, R.F., Pflieger, W.L. & Robison, H.W. (1996) Conservation status of crayfishes of the United States and Canada. Fisheries, 21, 25–38. https://doi.org/10.1577/1548-8446(1996)021%3C0025:CSOCOT%3E2.0.CO;2 DOI: https://doi.org/10.1577/1548-8446(1996)021<0025:CSOCOT>2.0.CO;2
  25. Taylor, C.A., Schuster, G.A., Cooper, J.E., Distefano, R.J., Eversole, A.G., Hamr, P., Hobbs III., H.H., Robison, H.W., Skelton, C.E. & Thoma, R.F. (2007) A reassessment of the conservation status of crayfishes of the United States and Canada after 10+ years of increased awareness. Fisheries, 32 (8), 372–389. https://doi.org/10.1577/1548-8446(2007)32[372:AROTCS]2.0.CO;2 DOI: https://doi.org/10.1577/1548-8446(2007)32[372:AROTCS]2.0.CO;2