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Type: Article
Published: 2022-10-11
Page range: 125-142
Abstract views: 494
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A new species of bush frog (Anura, Rhacophoridae, Raorchestes) from southern Xizang, China

College of Life Sciences, Shenyang Normal University, Shenyang 110034, China 2Faculty of Agriculture, Forest and Food Engineering, Yibin University, Yibin 644007, China
Faculty of Agriculture, Forest and Food Engineering, Yibin University, Yibin 644007, China
Central South Inventory and Planning Institute of National Forestry and Grassland Administration, Changsha 410000, China
College of Life Sciences, Shenyang Normal University, Shenyang 110034, China 2Faculty of Agriculture, Forest and Food Engineering, Yibin University, Yibin 644007, China
Faculty of Agriculture, Forest and Food Engineering, Yibin University, Yibin 644007, China
Faculty of Agriculture, Forest and Food Engineering, Yibin University, Yibin 644007, China
College of Life Sciences, Shenyang Normal University, Shenyang 110034, China
Faculty of Agriculture, Forest and Food Engineering, Yibin University, Yibin 644007, China
Amphibia Himalayas morphology phylogeny Raorchestes yadongensis sp. nov. taxonomy

Abstract

Based on molecular evidence and morphological data, we describe a new species Raorchestes yadongensis sp. nov. from Yadong County, Xizang Autonomous Region, China. The new species can be distinguished based on a combination of the following characters: (1) small body size, SVL 17.8–24.1 mm in adult males; (2) head wider than long; (3) eye diameter about three times as much as tympanum diameter; (4) tympanum distinct; (5) fingers with rudimentary webbing and narrow lateral dermal fringes; relative finger lengths I < II < IV < III; number of subarticular tubercles in fingers 1, 1, 2, 1; (6) toes with rudimentary webbing and narrow lateral dermal fringes; relative toe lengths I < II < III < V < IV; number of subarticular tubercles in toes 1, 1, 2, 3, 2; inner metatarsal tubercle distinct, outer metatarsal tubercle absent; (7) tips of fingers and toes present discs, and discs pale brown or yellow in life; (8) tibiotarsal articulation reaching the tip of snout when adpressed; (9) milky nuptial pad present on the dorsal surface of first finger; (10) dorsal surface light brown with white warts, ventral surface with irregular white patches. The new species is currently known in Yadong County, Xizang, China, but may also occur in neighboring Bhutan and India. A key to Chinese species of the genus Raorchestes is also provided.

 

References

  1. Abraham, R.K., Pyron, R.A., Ansil, B.R., Zachariah, A. & Zachariah, A. (2013) Two novel genera and one new species of treefrog (Anura: Rhacophoridae) highlight cryptic diversity in the Western Ghats of India. Zootaxa, 3640 (2), 177–189. https://doi.org/10.11646/zootaxa.3640.2.3
    Al-Razi, H., Maria, M. & Muzaffar, S.B. (2020a) A new species of cryptic Bush frog (Anura, Rhacophoridae, Raorchestes) from northeastern Bangladesh. Zookeys, 927, 127–151. https://doi.org/10.3897/zookeys.927.48733
    Al-Razi, H., Maria, M., Hasan, S. & Muzaffar, S.B. (2020b) First record of Raorchestes longchuanensis Yang and Li, 1978 (Anura: Rhacophoridae) from northeastern Bangladesh suggests wide habitat tolerance. Amphibian & Reptile Conservation, 14 (1), 119–131.
    AmphibiaChina (2022) The Database of Chinese Amphibians. Available from: http://www.amphibiachina.org (accessed 7 September 2022)
    Anderson, J. (1878) Anatomical and Zoological Researches: Comprising an Account of the Zoological Results of the two Expeditions to Western Yunnan in 1868 and 1875, and a Monograph of the Two Cetacean Genera Platanista and Orcella. Vol. 1. Bernard Quaritch. London, pp. 703–860 + 969–975.
    Annandale, N. (1912) Zoological results of the Abor Expedition, 1911–1912. I. Amphibia. Records of the Indian Museum, 8, 7–36. https://doi.org/10.5962/bhl.part.1186
    Biju, S.D. & Bossuyt, F. (2009) Systematics and phylogeny of Philautus Gistel, 1848 (Anura, Rhacophoridae) in the Western Ghats of India, with descriptions of 12 new species. Zoological Journal of the Linnean Society, 155 (2), 374–444. https://doi.org/10.1111/j.1096-3642.2008.00466.x
    Biju, S.D., Shouche, Y., Dubois, A., Dutta, S.K. & Bossuyt, F. (2010) A ground-dwelling rhacophorid frog from the highest mountain peak of the Western Ghats of India. Current Science, 98, 1119–1125. https://doi.org/10.1371/journal.pone.0010331
    Boettger, O. (1895) Neue Frösche und Schlangen von den Liukiu-Inseln. Zoologischer Anzeiger, 18, 266–270.
    Bossuyt, F. & Dubois, A. (2001) A review of the frog genus Philautus Gistel, 1848 (Amphibia, Anura, Ranidae, Rhacophorinae). Zeylanica, 6 (1), 1–112.
    Boulenger, G.A. (1906) Description of two new Indian frogs. Journal of the Asiatic Society of Bengal, Series 2, 2, 385–386.
    Boulenger, G.A. (1919) Descriptions of three new batrachians from the Garo Hills, Assam. Records of the Indian Museum, 16, 207–208. https://doi.org/10.5962/bhl.part.25921
    Boulenger, G.A. (1893) Concluding report on the reptiles and batrachians obtained in Burma by Signor L. Fea dealing with the collection made in Pegu and the Karin Hills in 1887‒88. Annali del Museo Civico di Storia Naturale di Genova, Serie 2, 13, 304‒347.
    Chan, K.O., Grismer, L.L. & Brown, R.M. (2018) Comprehensive multi-locus phylogeny of Old World tree frogs (Anura: Rhacophoridae) reveals taxonomic uncertainties and potential cases of over and underestimation of species diversity. Molecular Phylogenetics and Evolution, 127, 1010–1019. https://doi.org/10.1016/j.ympev.2018.07.005
    Che, J., Jiang, K., Yan, F. & Zhang, Y.P. (2020) Amphibians and Reptiles in Tibet—Diversity and Evolution. Science Press, Beijing, 353pp.
    Chen, J.M., Prendini, E., Wu, Y.H., Zhang, B.L., Suwannapoom, C., Chen, H.M., Jin, J.Q., Lemmon, E.M., Lemmon, A.R., Stuart, B.L., Raxworthy, C.J., Murphy, R.W., Yuan, Z.Y. & Che, J. (2020) An integrative phylogenomic approach illuminates the evolutionary history of Old World tree frogs (Anura: Rhacophoridae). Molecular Phylogenetics and Evolution, 145, 106724. https://doi.org/10.1016/j.ympev.2019.106724
    Fei, L., Hu, S.Q., Ye, C.Y & Huang, Y.Z. (2009) Fauna Sinica. Amphibia. Vol.2. Anura. Science Press, Beijing, 957 pp.
    Fei, L., Ye, C.Y. & Jiang, J.P. (2012) Colored Atlas of Chinese Amphibians and their Distributions. Sichuan Publishing House of Science & Technology, Chengdu, 340 pp.
    Frost D.R. (2022) Amphibian Species of the World: An Online Reference. Version 6.0. American Museum of Natural History, New York, New York. Available from: http://research.amnh.org/herpetology/amphibia/index.html (accessed 16 September 2022)
    Garg, S., Suyesh, R., Das, S., Bee, M.A. & Biju, S.D. (2021) An integrative approach to infer systematic relationships and define species groups in the shrub frog genus Raorchestes, with description of five new species from the Western Ghats, India. PeerJ, 9, e10791. https://doi.org/10.7717/peerj.10791
    Gowande, G.G., Ganesh, S.R. & Mirza, Z.A. (2020) A new cryptic species of bush frog (Amphibia: Anura: Raorchestes) from the Southern Eastern Ghats, India. Taprobanica, 9 (2), 164–173 https://doi.org/10.47605/tapro.v9i2.229
    Hillis, D.M. & Bull, J.J. (1993) An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology, 42, 182–192.
    Jiang, K., Ren, J.L., Wang, J., Guo, J.F., Wang, Z., Liu, Y.H., Jiang, D.C. & Li, J.T. (2020) Taxonomic revision of Raorchestes menglaensis (Kou, 1990) (Amphibia: Anura), with descriptions of two new species from Yunnan, China. Asian Herpetological Research, 11 (4), 263–281. https://doi.org/10.16373/j.cnki.ahr.200018
    Jiang, K., Yan, F., Wang, K., Zou, D.H., Cheng, L.I. & Che, J. (2016) A new genus and species of treefrog from Medog, southeastern Tibet, China (Anura, Rhacophoridae). Zoological Research, 37 (1), 15–20. https://doi.org/10.13918/j.issn.2095-8137.2016.1.15
    Khatiwada, J.R., Wang, B., Zhao, T., Xie, F. & Jiang, J. (2021) An integrative taxonomy of amphibians of Nepal: An updated status and distribution. Asian Herpetological Research, 12 (1), 1–35. https://doi.org/10.16373/j.cnki.ahr.200050
    Kou, Z.T. (1990) A new species of genus Philautus (Amphibia: Rhacophoridae) from Yunnan, China. In: Zhao, E.M. (Ed.), From Water onto Land. China Forestry Press Beijing, pp. 210–212.
    Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33 (7), 1870–1874. https://doi.org/10.1093/molbev/msw054
    Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. & Calcott, B. (2017) PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution, 34 (3), 772–773. https://doi.org/10.1093/molbev/msw260
    Li, J.T., Che, J., Bain, R.H., Zhao, E.M. & Zhang, Y.P. (2008) Molecular phylogeny of Rhacophoridae (Anura): A framework of taxonomic reassignment of species within the genera Aquixalus, Chiromantis, Rhacophorus, and Philautus. Molecular Phylogenetics and Evolution, 48 (1), 302–312. https://doi.org/10.1016/j.ympev.2008.03.023
    Li, J.T., Che, J., Murphy, R.W., Zhao, H., Zhao, E.M., Rao, D.Q. & Zhang, Y.P. (2009) New insights to the molecular phylogenetics and generic assessment in the Rhacophoridae (Amphibia: Anura) based on five nuclear and three mitochondrial genes, with comments on the evolution of reproduction. Molecular Phylogenetics and Evolution, 53 (2), 509–522. https://doi.org/10.1016/j.ympev.2009.06.023
    Mathew, R. & Sen, N. (2009) Studies on little known amphibians of Northeast India. Records of the Zoological Survey of India, Occasional Papers, 293, 1–64.
    Minh, B.Q., Schmidt, H.A., Chernomor, O., Schrempf, D., Woodhams, M.D., Von Haeseler, A. & Lanfear, R. (2020) IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era. Molecular Biology and Evolution, 37 (5), 1530–1534. https://doi.org/10.1093/molbev/msaa015
    Padhye, A.D., Sayyed, A., Jadhav, A. & Dahanukar, N. (2013) Raorchestes ghatei, a new species of shrub frog (Anura: Rhacophoridae) from the Western Ghats of Maharashtra, India. Journal of Threatened Taxa, 5 (15), 4913–4931. https://doi.org/10.11609/JoTT.o3702.4913–31
    Penny, D. (2004) Phylogeny in the comfort zone. Systematic Biology, 53 (4), 669.
    Pillai, R.S. & Chanda, S.K. (1973) Philautus shillongensis, a new frog (Ranidae) from Meghalaya, India. Proceedings of the Indian Academy of Sciences B, 78 (1), 30–36.
    Palumbi, S.R., Martin, A., Romano, S., Owen MacMillan, W., Stice, L. & Grabowski, G. (1991) The Simple Fool’s Guide to PCR. Department of Zoology, University of Hawaii, Honolulu
    Thompson, J.D., Gibson, T.J. & Higgins, D.G. (2003) Multiple sequence alignment using ClustalW and ClustalX. Current Protocols in Bioinformatics, 1, 2–3. https://doi.org/10.1002/0471250953.bi0203s00
    Rambaut, A., Suchard, M.A., Xie, D. & Drummond, A.J. (2014) Tracer. Version 1.6. Available from: http://beast.bio.ed.ac.uk/Tracer (accessed 28 September 2022)
    Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61 (3), 539–542. https://doi.org/10.1093/sysbio/sys029
    Sarkar, A.K. & Ray, S. (2006) Amphibia. In: Alfred, J.R.B. (Ed.), Fauna of Arunachal Pradesh. Part 1. Zoological Survey of India, Calcutta, pp. 285–316.
    Sarkar, A.K. & Sanyal, D.P. (1985) Amphibia. Records of the Zoological Survey of India, 81, 285–295.
    Seshadri, K.S., Gururaja, K.V. & Aravind, N.A. (2012) A new species of Raorchestes (Amphibia: Anura: Rhacophoridae) from mid-elevation evergreen forests of the southern Western Ghats, India. Zootaxa, 3410 (1), 19–34. https://doi.org/10.11646/zootaxa.3410.1.2
    Smith, M.A. (1924) New treefrogs from Indo-China and the Malay Peninsula. Proceedings of the Zoological Society of London, 1924, 225–234. https://doi.org/10.1111/j.1096–3642.1924.tb01499.x
    Watters, J.L., Cummings, S.T., Flanagan, R.L. & Siler, C.D. (2016) Review of morphometric measurements used in anuran species descriptions and recommendations for a standardized approach. Zootaxa, 4072(4), 477–495. https://doi.org/10.11646/zootaxa.4072.4.6
    Wu, S.P., Huang, C.C., Tsai, C.L., Lin, T.E., Jhang, J.J. & Wu, S.H. (2016) Systematic revision of the Taiwanese genus Kurixalus members with a description of two new endemic species (Anura, Rhacophoridae). ZooKeys, 557, 121–153 https://doi.org/10.3897/zookeys.557.6131
    Wu, Y.H., Suwannapoom, C., Xu, K., Chen, J.M., Jin, J.Q., Chen, H.M., Murphy, R.W. & Che, J. (2019) A new species of the genus Raorchestes (Anura: Rhacophoridae) from Yunnan Province, China. Zoological Research, 40 (6), 558–563. https://doi.org/10.24272/j.issn.2095-8137.2019.066
    Wu, Y.H., Liu, X.L., Gao, W., Wang, Y., Li, Y.C., Zhou, W.W., Yuan, Z.Y. & Che, J. (2021) Description of a new species of bush frog (Anura: Rhacophoridae: Raorchestes) from northwestern Yunnan, China. Zootaxa, 4941 (2), 239–258. https://doi.org/10.11646/zootaxa.4941.2.5
    Vijayakumar, S.P., Dinesh, K.P., Prabhu, M.V. & Shanker, K. (2014) Lineage delimitation and description of nine new species of bush frogs (Anura: Raorchestes, Rhacophoridae) from the Western Ghats Escarpment. Zootaxa, 3893 (4), 451–488. https://doi.org/10.11646/zootaxa.3893.4.1
    Vijayakumar, S.P., Menezes, R.C., Jayarajan, A. & Shanker, K. (2016) Glaciations, gradients, and geography: multiple drivers of diversification of bush frogs in the Western Ghats escarpment. Proceedings of the Royal Society B: Biological Sciences, 283 (1836), 20161011. https://doi.org/10.1098/rspb.2016.1011
    Yang, D.T. & Li, S.M. (1979) New species and new subspecies of amphibians and reptiles from Gaoligong Shan,Yunnan. Acta Zootaxonomica Sinica, 4, 185–188.
    Yu, G., Rao, D., Yang, J. & Zhang, M. (2008) Phylogenetic relationships among Rhacophorinae (Rhacophoridae, Anura, Amphibia), with an emphasis on the Chinese species. Zoological Journal of the Linnean Society, 153 (4), 733–749. https://doi.org/10.1111/j.1096-3642.2008.00404.x
    Yu, G., Rao, D., Zhang, M. & Yang, J. (2009) Re-examination of the phylogeny of Rhacophoridae (Anura) based on mitochondrial and nuclear DNA. Molecular Phylogenetics and Evolution, 50 (3), 571–579. https://doi.org/10.1016/j.ympev.2008.11.023
    Zachariah, A., Dinesh, K.P., Kunhikrishnan, E., Das, S., Raju, D.V., Radhakrishnan, C., Palot, M.J. & Kalesh, S. (2011) Nine new species of frogs of the genus Raorchestes (Amphibia: Anura: Rhacophoridae) from southern Western Ghats, India. Biosystematica, 5 (1), 25–48.

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