Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2022-10-10
Page range: 24-50
Abstract views: 638
PDF downloaded: 52

A new species of Chiasmocleis Méhely, 1904 (Anura: Microhylidae) from the Peruvian Amazonia

Laboratório de Sistemática de Vertebrados, Pontifícia Universidade Católica do Rio Grande do Sul, Av. Ipiranga 6681, 90619-900, Porto Alegre, RS, Brazil.
Dirección de Investigación en Diversidad Biológica Terrestre Amazónica, Instituto de Investigaciones de la Amazonía Peruana, Av. Quiñones km 2.5, Iquitos, Loreto, Peru.
Dirección de Investigación en Diversidad Biológica Terrestre Amazónica, Instituto de Investigaciones de la Amazonía Peruana, Av. Quiñones km 2.5, Iquitos, Loreto, Peru.
Departmento de Zoología, Universidad de Sevilla, 41012, Seville, Spain.
Amphibia CT-scan morphology osteology phylogeny subgenus taxonomy

Abstract

We describe a new species of Chiasmocleis from the Amazonian forest of Peru. The new species is characterized by its medium size (snout-to-vent length = 18.2–20.8 mm in females, and 16.5 mm in one male), hands and feet with slightly developed fridges in females (more developed in male), and presence of a femoral line in all individuals. We also infer its phylogenetic position using DNA sequences of fragments of the mitochondrial genes for 16S rRNA and cytochrome oxidase subunit 1 (COI), and describe the mineralized skeleton through 3D models generated by computed tomography (CT-scan). Based on our results, we discuss the variation of some osteological characters traditionally used in the systematics of the genus.

 

References

  1. AmphibiaWeb (2022) AmphibiaWeb. University of California, Berkeley, California. Available from: https://amphibiaweb.org (accessed 18 February 2022)
    Almendáriz, A.C., Brito-M., J., Batallas-R., D., Vaca-Guerrero, J. & Ron, S.R. (2017) Una especie nueva de rana del género Chiasmocleis (Microhylidae: Gastroprhyninae) de la Cordillera del Cóndor, Ecuador. Papéis Avulsos de Zoologia, 57 (10), 119–136. https://doi.org/10.11606/0031-1049.2017.57.10
    Boettger, O. (1885) Liste von Reptilien und Batrachiern aus Paraguay. Zeitschrift für Naturwissenschaften. Halle, 58, 213–248.
    Canedo, C., Dixo, M. & Pombal Jr., J.P. (2004) A new species of Chiasmocleis Méhelÿ, 1904 (Anura, Microhylidae) from the Atlantic Rainforests of Bahia, Brazil. Herpetologica, 60 (4), 495–501. https://doi.org/10.1655/03-66
    Caramaschi, U. & Cruz, C.A.G. (1997) Redescription of Chiasmocleis albopunctata (Boettger) and description of a new species of Chiasmocleis (Anura: Microhylidae). Herpetologica, 53 (2), 259–268.
    Caramaschi, U. & Cruz, C.A.G. (2001) A new species of Chiasmocleis Méhelÿ, 1904 from Brazilian Amazonia (Amphibia, Anura, Microhylidae). Boletim do Museo Nacional, Nova Série, Zoologia, 469, 1–8.
    Caramaschi, U. (2010) Notes on the taxonomic status of Elachistocleis ovalis (Schneider, 1799) and description of five new species of Elachistocleis Parker, 1927 (Amphibia, Anura, Microhylidae). Boletim do Museu Nacional, Nova Serie, Zoologia, 527, 1–30.
    Cruz, C.A.G., Caramaschi, U. & Izecksohn, E. (1997) The genus Chiasmocleis Méhely, 1904 (Anura, Microhylidae) in the Atlantic Rain Forest of Brazil, with description of three new species. Alytes, 15 (2), 49–71.
    de Sá, R.O. & Trueb, L. (1991) Osteology, skeletal development, and chodrocranial structure of Hamptophryne boliviana (Anura: Microhylidae). Journal of Morphology, 209, 311–330. https://doi.org/10.1002/jmor.1052090307
    de Sá, R.O., Streicher, J.W., Sekonyela, R., Forlani, M.C., Loader, S.P., Greenbaum, E., Richards, S. & Haddad, C.F.B. (2012) Molecular phylogeny of microhylid frog (Anura: Microhylidae) with emphasis on relationships among New World genera. BioMed Central Evolutionary Biology, 12 (1), 241–262. https://doi.org/10.1186/1471-2148-12-241
    de Sá, R.O., Tonini, J.F.R., van Huss, H., Long, A., Cuddy, T., Forlani, M.C., Peloso, P.L.V., Zaher, H. & Haddad, C.F.B. (2019a) Multiple connections between Amazonia and Atlantic Forest shaped the phylogenetic and morphological diversity of Chiasmocleis Mehely, 1904 (Anura: Microhylidae: Gastrophryninae). Molecular Phylogenetics and Evolution, 130, 198–210. https://doi.org/10.1016/j.ympev.2018.10.021
    de Sá, R.O., Tonini, J.F.R., van Huss, H., Zaher, H. & Haddad, C.F.B. (2019b) The unique traits of the subgenus Unicus within Chiasmocleis Méhely, 1904 (Anura: Microhylidae). Zootaxa, 4646 (3), 585–590. https://doi.org/10.11646/zootaxa.4646.3.8
    Duellman, W.E. & Mendelson, J.R. (1995) Amphibians and reptiles from northern departamento Loreto, Peru: taxonomy and biogeography. The University of Kansas Science Bulletin, 55 (10), 329–376. https://doi.org/10.5962/bhl.part.779
    Duellman, W.E. (2005) Cusco Amazónico: The lives of amphibians and reptiles in an Amazonian rainforests. 1st Edition. Cornell University Press, New Yor,, New York, 433 pp.
    Dubois, A., Frétey, T. & Ohler, A. (2018) The Relictus case: it is high time that taxonomist follow the Code’s requirements for nomenclatural availability and validity of new zoological nomina. Bionomina, 13, 51–64. https://doi.org/10.11646/bionomina.13.1.4
    Dubois, A., Ohler, A. & Pyron, R.A. (2021) New concepts and methods for phylogenetic taxonomy and nomenclature in zoology, exemplified by a new ranked cladomy of recent amphibians (Lissamphia). Megataxa, 5 (1), 1–738. https://doi.org/10.11646/megataxa.5.1.1
    Dunn, E.R. (1949) Notes on South American frogs of the family Microhylidae. American Museum Novitates, 1419, 1–21.
    Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32 (5), 1792–1797. https://doi.org/10.1093/nar/gkh340
    Forlani, M.C., Tonini, J.F.R., Cruz, C.A.G., Zaher, H. & de Sá, R.O. (2017) Molecular and morphological data reveal three new cryptic species of Chiasmocleis (Mehely 1904) (Anura, Microhylidae) endemic to the Atlantic Forest, Brazil. PeerJ, 5, e3005. https://doi.org/10.7717/peerj.3005
    Fouquet, A., Leblanc, K., Fabre, A-C., Rodrigues, M.T., Menin, M., Courtois, E.A., Dewynter, M., Hölting, M., Ernst, R., Peloso, P. & Kok, P.J. (2021) Comparative osteology of fossorial frogs of the genus Synapturanus (Anura, Microhylidae) with the description of three new species from the Eastern Guiana Shield. Zoologischer Anzeiger, 203, 46–73. https://doi.org/10.1016/j.jcz.2021.05.003
    Funk, W.C. & Cannatella, D.C. (2009) A new, large species of Chaismocleis Méhelÿ 1904 (Anura: Microhylidae) from the Iquitos region, Amazonian Peru. Zootaxa, 2247 (1), 37–50. https://doi.org/10.11646/zootaxa.2247.1.2
    Frost, D.R. (2022) Amphibian Species of the World: an online reference. Version 6.1. Available from https://amphibiansoftheworld.amnh.org/ (accessed 18 February 2022)
    Gagliardi-Urrutia, G., Odicio, M.I. & Venegas, P.J. Anfibios y reptiles/Amphibians and reptiles. In: Pitman, N., Vriesendorp, C., Rivera Chávez, L., Wachter, T., Alvira Reyes, D., del Campo, Á., Gagliardi-Urrutia, G., Rivera González, D., Trevejo, L., Rivera González, D. & Heipern, S. (Eds.), Perú: Tapiche-Blanco. Rapid Biological and Social Inventories Report 27. The Field Museum, Chicago, Illinois, pp. 117–125 + 297–305 + 436–445.
    Jenkins, C.N., Pimm S.L. & Joppa, L.N. (2013) Global patterns of terrestrial vertebrate diversity and conservation. Proceedings of the National Academy of Sciences, 110 (28), e2602–e 2610. https://doi.org/10.1073/pnas.1302251110
    Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K.F., von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods, 14, 587–589. https://doi.org/10.1038/nmeth.4285
    Katoh, K., Rozewicki, J. & Yamada, K.D. (2017) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics, 20 (4), 1160–1166. https://doi.org/10.1093/bib/bbx108
    Krügel, P. & Richter, S. (1995) Syncope antenori: a bromeliad breeding frog with free-swimming, nonfeeding tadpoles (Anura, Microhylidae). Copeia, 4, 955–963. https://doi.org/10.2307/1447045
    Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35 (6), 1547 – 1549. https://doi.org/10.1093/molbev/msy096
    Larsson, A. (2014) Aliview: a fast and lightweight alignment viewer and editor for large data sets. Bioinformatics, 30 (22), 3276–3278. https://doi.org/10.1093/bioinformatics/btu531
    Lyra, M.L., Haddad, C.F.B. & Azeredo-Espin, A.M.L. (2017) Meeting the challenge of DNA barcoding Neotropical amphibians: polymerase chain reaction optimization and new COI primers. Molecular Ecology Resources, 17, 966–980. https://doi.org/10.1111/1755-0998.12648
    Minh, B.Q., Schmidt, H.A., Chernomor, O., Schrempf, D., Woodhams, M.D., von Haeseler, A. & Lafear, R. (2020) IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era. Molecular Biology and Evolution, 37 (5), 1530 – 1534. https://doi.org/10.1093/molbev/msaa131
    Morales, V.R. & McDiarmid, R.W. (2009) A new species of Chiasmocleis (Anura: Microhylidae) from southern Amazonian Peru with comments on some other microhlids. Biotempo, 9, 71–76. https://doi.org/10.31381/biotempo.v9i0.69
    Moravec, J. & Köhler, J. (2007) A new species of Chiasmocleis (Anura: Microhylidae) from the Iquitos region, Amazonian Peru, with possible direct development. Zootaxa, 1605 (1), 59–67. https://doi.org/10.11646/zootaxa.1605.1.4
    Méhely, L. (1904) Investigations on Paraguayan batrachians. Annales Historico-Naturales Musei Nationalis Hungarici, 2, 207 –232.
    Nascimento, F.A.C., Vilela, B., Dubeux, M.J.M., Galdino, J.Y.A., Araújo-Neto, J.V., Leal, F. & de Sá, R. (2020) Reproductive biology and sexual dimorphism of the poorly know frog Chiasmocleis alagoana (Microhylidae, Gastrophryninae), with an updated diagnosis for the species. Studies on the Neotropical Fauna and Environment, 57 (2), 96–110. https://doi.org/10.1080/01650521.2020.1815942
    Nelson, C.E. (1975) Another new miniature 4-toed South American Microhylid frog (Genus: Syncope). Journal of Herpetology, 9 (1), 81–84. https://doi.org/10.2307/1562693
    Palumbi, S.R., Martin, A., Romano, S., McMillan, W.O., Stice, L. & Grabowski, G. (1991) The simple fool’s guide to PCR. Version 2.0. Privately published document compiled by S. Palumbi, Dept. Zoology, University of Hawaii, Honolulu, 45 pp.
    Parker, H.W. (1927) The brevicipitid frogs allied to the genus Gastrophryne. Occasional Papers of the Museum of Zoology University of Michigan, 187, 1–6.
    Parker, H.W. (1940) Undescribed anatomical structure and new species of reptiles and amphibians. Annals and Magazine of Natural History, 11 (5), 257–274. https://doi.org/10.1080/00222934008527045
    Peloso, P.L.V. & Sturaro, M.J. (2008) A new species of narrow-mouthed frog of the genus Chiasmocleis Méhelÿ 1904 (Anura, Microhylidae) from the Amazonian rainforest of Brazil. Zootaxa, 1947, 39–52.
    Peloso, P.L.V., Sturaro, M.J, Forlani, M.C., Gaucher, P., Motta, A.P. & Wheeler, C. (2014) Phylogeny, taxonomic revision, and character evolution of the genera Chiasmocleis and Syncope (Anura, Microhylidae) in Amazonia, with descriptions of three new species. Bulletin of the American Museum of Natural History, 386, 1–96. https://doi.org/10.1206/834.1
    Peloso, P.L.V., Frost, D.R., Richards, S.J., Rodrigues, M.T., Donnellan, S., Matsui, M., Raxworthy, C.J., Biju, S.D., Lemmon, E.M., Lemmon, A.R. & Wheeler, W.C. (2016) The impact of anchored phylogenomics and taxon sampling on phylogenetic inference in narrow-mouthed frogs (Anura, Microhylidae) Cladistics 32, 113 – 140. https://doi.org/10.1111/cla.12118
    Pérez-Peña, P.E., Tapia-del Aguila, C.J. & Gagliardi-Urrutia, G. (2021). Reproducción, distribución y hábitats de Chiasmocleis magnova Moravec y Köhler, 2007 (Anura: Microhylidae). Folia Amazonica, 30 (2), 149–165. https://doi.org/10.24841/fa.v30i2.588
    Rodriguez, L.O. & Duellan, W.E. (1994) Guide of the frogs of the Iquitos region Amazonina Peru. The University of Kansas Natural History Museum Special Publication 22. The University of Kansas Natural History Museum, Lawrence, Kansas, 88 pp., 12 pls.
    Rios-Alva, E.J., Rojas-Padilla, O. & Gagliardi-Urrutia, G. (2015) Anfibios y reptiles. In: García-Villacorta, R. (Eds.), Inventario Biológica en los Bosques Estacionalmente Secos del Huallaga Central, San Martín, Perú. Instituto de Investigaciones de la Amazonía Peruana, Iquitos, pp. 82–98.
    Scherz, M.D., Hawlitscheck, O., Andreone, F., Rakotoarison, A., Vences, M. & Glaw, F. (2017) A review of the taxonomy and osteology of the Rhombophryne serratopalpebrosa species group (Anura: Microhylidae) from Madagascar, with comments on the value of volume rendering of micro-CT data to taxonomists. Zootaxa, 4273 (3), 301–340. https://doi.org/10.11646/zootaxa.4273.3.1
    Trifininopoulos, J., Nguyen, L.-T., von Haeseler, A. & Minh, B.Q. (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research, 44 (W1), W232–W235. https://doi.org/10.1093/nar/gkw256
    Trueb, L., Diaz, R. & Blackburn, D.C. (2011) Osteology and chondocranial morphology of Gastrophryne carolinensis (Anura: Microhylidae), with a review of the osteological diversity of New World microhylids. Phyllomedusa, 10 (2), 99–135. https://doi.org/10.11606/issn.2316-9079.v10i2p99-135
    Tu, N., Yang, M., Liang & D., Zhanga, P. (2018) A large-scale phylogeny of Microhylidae inferred from a combined dataset of 121 genes and 427 taxa. Molecular Phylogenetis and Evolution, 126, 85–91. https://doi.org/10.1016/j.ympev.2018.03.036
    Vaidya, G., Lohman, D.J. & Meier, R. (2011) SequenceMatrix: concatenation software for the fast assembly of multi-gene datasets with characer set and codon information. Cladistics, 27, 171–180. https://doi.org/10.1111/j.1096-0031.2010.00329.x
    Venegas, P.J., Gagliardi-Urrutia, G. & Odicio, M. (2014) Anfibios y reptiles/Amphibians and reptiles. In: Pitman, C., Vriesendorp, C., Alivra, D., Marrkel, J.A., Johnston, M., Ruelas Inzunza, E., Lancha Pizango, Sarmiento Valenzuela, G., Álvarez-Loayza P., Homan, J., Wachter, T., del Campo, Á., Stotz, D.F & Heilpern, S. (Eds.), Perú: Cordillera Escalera-Loreto. Rapid Biological and Social Inventories Report 26. The Field Museum, Chicago, pp. 127–138 + 319–329 + 470–481.
    Walker, C.F. (1973) A new genus and species of Microhylid frog from Ecuador. Occasional Papers of the Museum of Natural History, The University of Kansas, 20, 1–7. https://doi.org/10.5962/bhl.part.21780
    Walker, C.F. & Duellman, W.E. (1974) Description of a new species of microhylid frog, Chiasmocleis, from Ecuador. Occasional Papers of the Museum of Natural History, University of Kansas, 26, 1–6. https://doi.org/10.5962/bhl.part.4068
    Zweifel, R.G. (1986) A new genus and species of microhylid frog from the Cerro de la Neblina region of Venezuela and discussion of relationships among New World Microhylid genera. American Museum Novitates, 2863, 1–24.

  2.