Skip to main content Skip to main navigation menu Skip to site footer
Article
Published: 2022-06-08

A new species of Xenophrys (Amphibia: Anura: Megophryidae) from Truong Son Range, Vietnam

Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 10072, Vietnam;
Forest Resources and Environment Center, 300 Ngoc Hoi Road, Thanh Tri, Hanoi 10000, Vietnam;
Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 10072, Vietnam. Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 10072, Vietnam;
Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 10072, Vietnam. Institute of Genome Research, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 10072, Vietnam;
Zoological Institute, Russian Academy of Sciences, Universitetskaya nab 1, St. Petersburg 199034, Russia;
AG Zoologischer Garten Köln, Riehler Strasse 173, D-50735 Cologne, Germany;
Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 10072, Vietnam. Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 10072, Vietnam;
Amphibia Xenophrys truongsonensis sp. nov. molecular phylogeny morphology taxonomy

Abstract

A new species of Xenophrys is described from Truong Son Range in Central Vietnam based on morphological and molecular differences. The new species is distinguishable from its congeners by a combination of the following characters: Size medium (SVL 58.8–71.4 mm in males, 65.6–87.3 mm in females); tympanum distinct; vomerine teeth present; tongue notched posteriorly; external vocal sac absent; toes with rudimentary webbing; subarticular tubercles absent, lateral fringes narrow along toes; nuptial pads absent in males; dorsal skin of body with weak granules; flank with small tubercles; dorsum with a dorsal ridge in >-< shape; dorsolateral folds prominent; dorsal surface from reddish brown to grayish brown with a brown triangle between the eyes, and a dark brown marking edged along dorsal ridge on the back; and small white spots present on flank and back of thigh. In the phylogenetic analyses, the new species is nested within the genus Xenophrys with interspecific uncorrected genetic p-distances (16S rRNA gene) from 2.2–2.8% (compared with Xenophrys maosonensis) to 14.4–14.7% (compared with Xenophrys yeae).

 

References


  1. Boulenger, G.A. (1908) A revision of the oriental pelobatid batrachians (genus Megalophrys). Proceedings of the Zoological Society of London, 1908, 407–430. https://doi.org/10.1111/j.1096-3642.1908.tb01852.x
    Bourret, R. (1937) Notes herpétologiques sur l’Indochine française. XIV. Les batraciens de la collection du Laboratoire des Sciences Naturelles de l’Université. Descriptions de quinze especes ou variétés nouvelles. Annexe au Bulletin Général de l’Instruction Publique, 1937, 5–56.
    Bourret, R. (1942) Les Batraciens de l’Indochine. Institut Océanographique de l’Indochine, Hanoi, 567 pp.
    Chen, J., Zhou, W., Poyarkov, N.A., Stuart, B.L., Brown, R.M., Lathrop, A., Wang, Y., Yuan, Z., Jiang, K., Hou, M., Chen, H., Suwannapoom, C, Nguyen, S.N., Duong, T.V., Papenfuss, T.J., Murphy, R.W., Zhang, Y. & Che, J. (2017) A novel multilocus phylogenetic estimation reveals unrecognized diversity in Asian horned toads, genus Megophrys sensu lato (Anura: Megophryidae). Molecular Phylogenetics and Evolution, 106, 28–43. https://doi.org/10.1016/j.ympev.2016.09.004
    Dubois, A., Ohler, A. & Pyron, R.A. (2021) New concepts and methods for phylogenetic taxonomy and nomenclature in zoology, exemplified by a new ranked cladonomy of recent amphibians (Lissamphibia). Megataxa, 5 (1), 1–738. https://doi.org/10.11646/megataxa.5.1.1
    Fei, L., Hu, S., Ye, C. & Huang, Y. (2009) Fauna Sinica. Amphibia. Vol. 2. Anura. Chinese Academy of Science, Science Press, Beijing, 957 pp. [in Chinese]
    Fei, L. & Ye, C. (2016) Amphibians of China. Vol. 1. Chengdu Institute of Biology, Chinese Academy of Sciences, Science Press, Beijing, 1040 pp.
    Fei, L., Ye, C.Y. & Huang, Y. (1992) Description of two new species of the genus Megophrys, Pelobatidae (Amphibia: Anura) from China. Zoological Research, 13, 5–12.
    Fei, L., Ye, C. & Jiang, J. (2010) Colored Atlas of Chinese Amphibians. Sichuan Publishing House of Science & Technology, Sichuan, 519 pp. [in Chinese]
    Frost, D.R. (2022) Amphibian Species of the World: an online reference. Version 6.0. American Museum of Natural History, New York, New York. Available from: http://research.amnh.org/herpetology/amphibia/index.html (assessed 30 January 2022) https://doi.org/10.5531/db.vz.0001
    Glaw, F. & Vences, M. (2007) A field guide to the amphibians and reptiles of Madagascar. 3rd Edition. Frosch Verlag, Cologne, 496 pp.
    Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In: Nucleic acids symposium series. Information Retrieval, 1999, 95–98.
    Hammer, Ø., Harper, D.A.T. & Ryan, P.D. (2001) PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica, 4 (1), 1–9. Available from: http://palaeo-electronica.org/2001_1/past/issue1_01.htm (assessed 30 May 2021)
    Hoang, C.V., Nguyen, T.T., Luu, V.Q., Nguyen, T.Q. & Jiang, J.P. (2019) A new species of Leptobrachella Smith 1925 (Anura: Megophryidae) from Thanh Hoa Province, Vietnam. Raffles Bulletin of Zoology, 67, 536–556.
    https://doi.org/10.26107/RBZ-2019-0042
    Huang, C.M., Lathrop, A. & Murphy, R.W. (1998) Translations of “Two new species of amphibian in Tibet” Huang & Fei 1981 and “Description of two new species of the genus Megophrys” Fei, Ye, & Huang 1995. Smithsonian Herpetological Information Service, 118, 1–20.
    Huang, Y.Z. & Fei, L. (1981) Two new species of amphibians from Xizang. Acta Zootaxonomica Sinica/Dong Wu Fen Lei Xue Bao, Beijing, 6, 211–215.
    Huelsenbeck, J.P. & Hillis, D.M. (1993) Success of phylogenetic methods in the four-taxon case. Systematic Biology, 42 (3), 247–264. https://doi.org/10.2307/2992463
    Jobb, G. (2011) Treefinder Version of March 2011. Software Distributed by the Author. Available from: http://www.treefinder.de (assessed 30 August 2021)
    Kimura, M. (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120.
    Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35, 1547–1549. https://doi.org/10.1093/molbev/msy096
    Leaché, A.D. & Reeder, T.W. (2002) Molecular systematics of the eastern fence lizard (Sceloporus undulatus): a comparison of parsimony, likelihood, and Bayesian approaches. Systematic Biology, 51, 44–68.
    Mahony, S. (2011) Two new species of Megophrys Kuhl & van Hasselt (Amphibia: Megophryidae), from western Thailand and southern Cambodia. Zootaxa, 2734 (1), 23–39. https://doi.org/10.11646/zootaxa.2734.1.2
    Mahony, S., Kamei, R.G., Teeling, E.C. & Biju, S.D. (2018) Cryptic diversity within the Megophrys major species group (Amphibia: Megophryidae) of the Asian Horned Frogs: Phylogenetic perspectives and a taxonomic revision of South Asian taxa, with descriptions of four new species. Zootaxa, 4523 (1), 1–96. https://doi.org/10.11646/zootaxa.4523.1.1
    Mahony, S., Sengupta, S., Kamei, R.G. & Biju, S.D. (2011) A new low altitude species of Megophrys Kuhl & van Hasselt (Amphibia: Megophryidae), from Assam, Northeast India. Zootaxa, 3059 (1), 36–46. https://doi.org/10.11646/zootaxa.3059.1.2
    Mahony, S., Teeling, E.C. & Biju, S.D. (2013) Three new species of horned frogs, Megophrys (Amphibia: Megophryidae), from northeast India, with a resolution to the identity of Megophrys boettgeri populations reported from the region. Zootaxa, 3722 (2), 143–169. https://doi.org/10.11646/zootaxa.3931.2.3
    Manthey, U. & Grossmann, W. (1997) Amphibien and Reptilien Südostasiens. Natur und Tier Verlag, Münster, 512 pp.
    Neang, T., Chin, S., Meang, M. & Hun, S. (2013) Confirmation of three species of megophryid frogs (Amphibia: Megophryidae) from the Cardamom Mountains of Southwest Cambodia, with the rediscovery of a long lost species. Cambodian Journal of Natural History, 2013 (2), 66–72.
    Nguyen, T.Q., Pham, C.T., Nguyen, T.T., Luong, A.M. & Ziegler, T. (2020) A new species of Megophrys (Amphibia: Anura: Megophryidae) from Vietnam. Zootaxa, 4722 (5), 401–422. https://doi.org/10.11646/zootaxa.4722.5.1
    Nguyen, T.T., Pham, T.C., Nguyen, T.Q., Ninh, H.T. & Ziegler, T. (2017) A new species of Rhacophorus (Amphibia: Anura: Rhacophoridae) from Vietnam. Asian Herpetological Research, 8, 221–234. https://doi.org/10.16373/j.cnki.ahr.170046
    Ohler, A., Swan, S.R. & Daltry, J.C. (2002) A recent survey of the amphibian fauna of the Cardomom Mountains, Southwest Cambodia with descriptions of three new species. Raffles Bulletin of Zoology, 50 (2), 465–481.
    Poyarkov, N.A. Jr., Nguyen, T.V., Popov, E.S., Geissler, P., Pawangkhanant, P., Neang, T., Suwannapoom, C. & Orlov, N.L. (2021) Recent progress in taxonomic studies, biogeographic analysis, and revised checklist of amphibians of Indochina. Russian Journal of Herpetology, 28, 1–110. https://doi.org/10.30906/1026-2296-2021-28-3A-1-110
    Qi, S., Lyu, Z.T., Wang, J., Mo, Y.M., Zeng, Z., Zeng, Y.J., Dai, K.Y., Li, Y.Q., Grismer, L.L. & Wang., Y.Y. (2021) Three new species of the genus Boulenophrys (Anura, Megophryidae) from southern China. Zootaxa, 5072 (5), 401–438. https://doi.org/10.11646/zootaxa.5072.5.1
    Rambaut, A., Drummond, A.J., Xie, D., Baele, G. & Suchard, M.A. (2018) Posterior summarisation in Bayesian phylogenetics using Tracer 1.7. Systematic Biology, 67, 901–904. https://doi.org/10.1093/sysbio/syy032
    Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574. https://doi.org/10.1093/bioinformatics/btg180
    Shi, S., Zhang, M., Xie, F., Jiang, J., Liu, W., Ding, L., Luan, L. & Wang, B. (2020) Multiple data revealed two new species of the Asian horned toad Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from the eastern corner of the Himalayas. ZooKeys, 977, 101–161 https://doi.org/10.3897/zookeys.977.55693
    Simmons, J.E. (2002) Herpetological collecting and collections management. Revised edition. Society for the Study of Amphibians and Reptiles. Herpetological Circulars, 31, 1–153.
    Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers, Annals of the Entomological Society of America, 87, 651–701.
    Stuart, B.L., Chuaynkern, Y., Chan-ard, T. & Inger, R.F. (2006) Three new species of frogs and a new tadpole from eastern Thailand. Fieldiana, 111, 1–19.
    Tamura, K. (1992) Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Molecular Biology and Evolution, 9, 678–687.
    Tanabe, A.S. (2011) Kakusan 4 and Aminosan: two programs for comparing nonpartitioned, proportional and separate models for combined molecular phylogenetic analyses of multilocus sequence data. Molecular Ecology Resources, 11, 914–921.
    Taylor, E.H. (1962) The amphibian fauna of Thailand. University of Kansas Science Bulletin 43. University of Kansas, Lawrence, Kansas, pp. 265–599. https://doi.org/10.5962/bhl.part.13347
    Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic acids research, 25, 4876–4882. https://doi.org/10.1093/nar/25.24.4876