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Type: Article
Published: 2021-12-20
Page range: 322-340
Abstract views: 418
PDF downloaded: 28

Cambarus ectopistes sp. nov., a new stream-dwelling crayfish (Decapoda: Cambaridae) from the French Broad, Pigeon, and Nolichucky River watersheds in the Appalachian Mountain region of North Carolina and Tennessee, USA

West Liberty University, Department of Biological Sciences, C.S.C Box 139, West Liberty, West Virginia, 26074.
North Carolina Museum of Natural Sciences, Research Laboratory, 1671 Gold Star Drive, Raleigh, NC 27669.
Crustacea Cambarus robustus Nolichucky River Pigeon River species complex

Abstract

The Cataloochee Crayfish, Cambarus ectopistes sp. nov., is a large, stream-dwelling crayfish that occupies a narrow noncontiguous distribution within the Appalachian Mountain region running through the Upper Tennessee River basin, in the French Broad, Pigeon, and Nolichucky watersheds along the Tennessee and North Carolina border. It is split from the southernmost extent of the C. robustus species complex, and is morphologically and genetically most similar to a second undescribed member of the group endemic to the upper Nolichucky River watershed in North Carolina. Cambarus ectopistes sp. nov. can be distinguished from other members of the C. robustus complex and co-distributed congeners by a combination of characters, including body size, coloration, and morphology of the chela and rostrum. The new species is typically found in channel and edge habitats of moderate to large perennial streams with large cobbles and boulders.

 

References

  1. Beck, M.W. (2018) ggord: Ordination plots with ggplot2. R package version 1.1.1. [software]
    Choate, E.A. (1985) The dictionary of American bird names, revised edition. The Harvard Common Press, Boston, Massachusetts.
    Cooper, J.E. (2006) A new species of crayfish of the genus Cambarus Erichson, 1846 (Decapoda: Cambaridae) from the eastern Blue Ridge Foothills and Western Piedmont Plateau of North Carolina. Proceedings of the Biological Society of Washington, 119, 67–80. https://doi.org/10.2988/0006-324X(2006)119[67:ANSOCO]2.0.CO;2
    Ewing, T.D., Thoma, R.F., Fraley, S.J., Russ, W.T. & Pope, J. (2016) Distribution and conservation status of the Grandfather Mountain Crayfish. Journal of the Southeastern Association of Fish and Wildlife Agencies, 3, 64–70.
    Folmer, O.F., Black, M.B., Hoeh, W.R. & Vrijenhoek, R.C. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.
    García-Melo, J.E., Oliveira, C, DaC osta Silva G.J., Ochoa-Orrego, L.E., Garcia Pereira, L.H. & Maldonado-Ocampo, J.A. (2019) Species delimitation of neotropical Characins (Stevardiinae): Implications for taxonomy of complex groups. PLoS ONE, 14, e0216786. https://doi.org/10.1371/journal.pone.0216786
    Glon, M.G., Thoma, R.F., Daly, M. & Freudenstein, J.V. (2019) Lacunicambarus chimera: a new species of burrowing crayfish (Decapoda: Cambaridae) from Illinois, Indiana, Kentucky, and Tennessee. Zootaxa, 4544 (4), 451–478. https://doi.org/10.11646/zootaxa.4544.4.1
    Guindon, S., Dufayard, J. F., Lefort, V., Anisimova, M., Hordijk, W. & Gascuel, O. (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology, 59, 307–321. https://doi.org/10.1093/sysbio/syq010
    Hobbs, H.H. Jr. (1969) On the distribution and phylogeny of the crayfish genus Cambarus. in: Holt, P.C., Hoffman, R.L. & Hart, C.W. Jr. (Eds.), The distributional history of the biota of the southern Appalachians, Part I: Invertebrates. Blacksburg. Monograph 1. Virginia Polytechnic Institute and State University, Research Division, Blacksburg, Virginia, pp. 93–178
    Hobbs, H.H. Jr. (1981) The crayfishes of Georgia. Smithsonian Contributions to Zoology, 318, 1–549. https://doi.org/10.5479/si.00810282.318
    Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K.F., von Haeseler, A., & Jermiin, L.S. (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods, 14, 587–589. https://doi.org/10.1038/nmeth.4285
    Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution, 30, 772–780. https://doi.org/10.1093/molbev/mst010
    Katoh, K., Misawa, K., Kuma, K. & Miyata, T. (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research, 30, 3059–3066. https://doi.org/10.1093/nar/gkf436
    Lanfear, R., Calcott, B., Ho, S.Y.W. & Guindon, S. (2012) PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution, 29, 1695–1701. https://doi.org/10.1093/molbev/mss020
    Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. & Calcott, B. (2016) PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution, msw260. https://doi.org/10.1093/molbev/msw260
    Li, M., Zhao, Q., Chen, R., He, J., Peng, T., Deng, W., Che, Y. & Wang, Z. (2020) Species diversity revealed in Sigmella Hebard, 1929 (Blattodea, Ectobiidae) based on morphology and four molecular species delimitation methods. PloS one, 15, p.e0232821. https://doi.org/10.1371/journal.pone.0232821
    Loughman, Z.J. & Fetzner, J.W. (2015) Astacology and crayfish conservation in the southeastern United States: past, present and future. Freshwater Crayfish, 21, 1–5. https://doi.org/10.5869/fc.2015.v21-1.1
    Loughman, Z.J., Henkanaththegedara, S.M., Fetzner Jr., J.W. & Thoma, R.F. (2017) A case of Appalachian endemism: Revision of the Cambarus robustus complex (Decapoda: Cambaridae) in the Kentucky and Licking River basins of Kentucky, USA, with the description of three new species. Zootaxa, 4269 (4), 460–494. https://doi.org/10.11646/zootaxa.4269.4.4
    Loughman, Z.J. & Williams, B.W. (2018) Cambarus polypilosus, a new species of stream-dwelling crayfish (Decapoda: Cambaridae) from the Western Highland Rim of Tennessee, USA. Zootaxa, 4403 (1), 171–185. https://doi.org/10.11646/zootaxa.4403.1.10
    McCune, B., Grace, J.B. & Urban, D.L. (2002) Analysis of ecological communities. MjM software design, Gleneden Beach, Oregon, 300 pp.
    Masters, B.C., Fan, V. & Ross, H.A. (2011) Species Delimitation—a Geneious plugin for the exploration of species boundaries. Molecular Ecology Resources, 11 (1), 154–157.
    https://doi.org/10.1111/j.1755-0998.2010.02896.x
    Minh, B.Q., Nguyen, M.A.T. & von Haeseler, A. (2013) Ultrafast approximation for phylogenetic bootstrap. Molecular Biology and Evolution, 30, 1188–1195. https://doi.org/10.1093/molbev/mst024
    Nguyen, L-T., Schmidt, H.A., von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution, 32, 268–274. https://doi.org/10.1093/molbev/msu300
    Oksanen, J., Blanchet, F.G., Friendly, M., Kindt, R., Legendre, P., McGlinn, D., Minchin, P.R., O’Hara, R.B., Simpson, G.L., Solymos, P., Stevens, M.H.H., Szoecs, E. & Wagner, H. (2018) vegan: Community Ecology Package. R package. Version 2.5-2. Available from: https://CRAN.R-project.org/package=vegan (accessed 22 January 2019)
    Richman, N.I., Böhm, M., Adams, S.B., Alvarez, F., Bergey, E.A., Bunn, J.J.S., Burnham, Q., Cordeiro, J., Coughran, J., Crandall, K.A., Dawkins, K.L., DiStefano, R.J., Doran, N.E., Edsman, L., Eversole, A.G., Fureder, L., Furse, J.M., Gherardi, F., Hamr, P., Holdich, D.M., Horwitz, P., Johnston, K., Jones, C.M., Jones, J.P., Jones, R.L., Jones, T.G., Kawai, T., Lawler, S., López-Mejía, M., Miller, R.M., Pedraza- Lara, C., Reynolds, J.D., Richardson, A.M., Schultz, M.B., Schuster, G.A., Sibley, P.J., Souty-Grosset, C., Taylor, C.A., Thoma, R.F., Walls, J., Walsh, T.S. & Collen, B. (2015) Multiple drivers of decline in the global status of freshwater crayfish (Decapoda: Astacidea). Philosophical Transactions of the Royal Society of London B, 370, 20140060. https://doi.org/10.1098/rstb.2014.0060
    Simmons, J.W. & Fraley, S.J. (2010) Distribution, Status, and Life-history Observations of Crayfishes in Western North Carolina. Conservation, Biology, and Natural History of Crayfishes from the Southern US. Southeastern Naturalist, 9, 79–126. https://doi.org/10.1656/058.009.s316
    Taylor, C.A., Warren Jr., M.L., Fitzpatrick Jr., J.F., Hobbs III, H.H., Jezerinac, R.F., Pflieger, W.L. & Robison, H.W. (1996) Conservation status of crayfishes of the United States and Canada. Fisheries, 21, 25–38. https://doi.org/10.1577/1548-8446(1996)021<0025:csocot>2.0.co;2
    Taylor, C.A., Schuster, G.A., Cooper, J.E., DiStefano, R.J., Eversole, A.G., Hamr, P., Hobbs III, H.H., Robison, H.W., Skelton, C.E. & Thoma, R.F. (2007) A reassessment of the conservation status of crayfishes of the United States and Canada after 10+ years of increased awareness. Fisheries, 32, 372–389. https://doi.org/10.1577/1548-8446(2007)32[372:arotcs]2.0.co;2
    Thoma, R.F. (2005) Cambarus (Cambarus) eeseeohensis (Decapoda: Cambaridae), a new species of crayfish from the Linville River of North Carolina and its bearing on understanding the evolution of the subgenus Cambarus. Proceedings of the Biological Society of Washington, 118, 794–802. https://doi.org/10.2988/0006-324x(2005)118[794:ccedca]2.0.co;2
    Wickham, H. (2016) ggplot2: elegant graphics for data analysis. Springer-Verlag, New York. [program]

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