Abstract
This publication describes a new species of mosquito of the genus Toxorhynchites Theobald, 1901, subgenus Lynchiella Lahille, 1904. The species was found during an exploratory survey of the Culicidae in the Caatinga biome, which is unique to Brazil and characterized by a semi-arid climate. Because of the specific characteristics of the biome, it has potential for endemism of species that have adapted to its restrictive conditions, particularly those imposed by severe drought. The new species, Toxorhynchites (Lynchiella) caatingensis, is a phytotelmic species that inhabits bromeliads and has an unknown biology. It belongs to a species complex that is hereafter referred to as the Violaceus Complex, which comprises three species that are found in the same type of habitat and have similar morphology: Tx. caatingensis n. sp., Tx. mariae (Bourroul, 1904) and Tx. violaceus (Wiedemann, 1820). We describe the larva, pupa, adult female and male genitalia of specimens collected as larvae from the tank bromeliad Aechmea aquilega (Salisb.) Griseb. The main distinctive characteristics of the species are the presence of three lateral tufts on the terminal abdominal segments (one pale and the others dark); a blue midline on the abdominal sterna; in the pupa, the pentagonal shape of the paddle and the length of seta 6-V. PCR reactions were carried out for the mitochondrial CO1 gene fragment, which placed the new species in the subgenus Lynchiella. No match was found with any species recorded in GenBank. The subgenus now includes 17 species.
References
Aditya, G., Ash, A. & Saha, G.K. (2006) Predatory activity of Rhantus sikkimensis and larvae of Toxorhynchites splendens on mosquito larvae in Darjeeling, India. Journal of Vector Borne Diseases, 43 (2), 66–72.
Albeny, D.S., Martins, G.F., Andrade, M.R., Krüger, R.F. & Vilela, E.F. (2011) Aedes aegypti survival in the presence of Toxorhynchites violaceus (Diptera: Culicidae) fourth instar larvae. Zoologia, 28 (4), 538–540. https://doi.org/10.1590/S1984-46702011000400017
Andrade, D.C., Morais, S.A., Marteis, L.S., Gama, R.A., de Mello Freire, R.C., Rekowski, B.S., Ueno, H.M. & La Corte, R. (2020) Diversity of mosquitoes (Diptera: Culicidae) in the Caatinga biome, Brazil, from the widespread to the endemic. Insects, 11 (8), 468. https://doi.org/10.3390/insects11080468
Belkin, J.N. (1968) Mosquito studies (Diptera, Culicidae). IX. The type specimens of New World mosquitoes in European museums. Contributions of the American Entomological Institute, 3 (4), 1‒69.
Belkin, J.N. (1977) Toxorhynchites (Lyn.) gerbergi, a new species from the southern Lesser Antilles. Mosquito Systematics, 9 (3), 329–332.
Belkin, J.N., Schick, R.X. & Heinemann, S.J. (1971) Mosquito studies (Diptera, Culicidae) XXV. Mosquitoes originally described from Brazil. Contributions of the American Entomological Institute, 7 (5), 1–66.
Berlin, O.G.W. (1969) Mosquito studies (Diptera, Culicidae) XII. A revision of the Neotropical subgenus Howardina of Aedes. Contributions of the American Entomological Institute, 4 (2), 1‒190.
Bonnet, D.D. & Hu, S.M.K. (1951) The introduction of Toxorhynchites brevipalpis Theobald into the territory of Hawaii. Proceedings of the Hawaiian Entomological Society, 14 (2), 237–242.
Bourroul, C. (1904) Mosquitos do Brasil. These de Doutoramento, Faculdade de Medicina da Bahia. Officina Typographica de João Baptista de Oliveira Costa, Bahia 50 pp. [Consisting of Prefacio (pp. i‒viii), Novas especies (pp. l‒6 + 30‒32), Quadro das especies encontradas na Bahia (4 pp.) and Proposições (18 pp.) by Bourroul; remainder and bulk of work by Adolpho Lutz. Continuous pagination used. See Belkin 1968, pp. 50 and 62 for details]
Climate-data.org (2017) Clima Jeremoabo (Brasil). https://pt.climate-data.org/location/42931/ (accessed 25 September 2017)
Collins, F.H. & Paskewitz, S.M. (1996) A review of the use of ribosomal DNA (rDNA) to differentiate among cryptic Anopheles species. Insect Molecular Biology, 5 (1), 1–9. https://doi.org/10.1111/j.1365-2583.1996.tb00034.x
Collins, L.E. & Blackwell, A. (2000) The biology of Toxorhynchites mosquitoes and their potential as biocontrol agents. Biocontrol News and Information, 21 (4), 105N–115N.
Corbet, P.S. & Griffiths, A. (1963) Observations on the aquatic stages of two species of Toxorhynchites (Diptera: Culicidae) in Uganda. Proceedings of the Royal Entomological Society of London, Series A, General Entomology, 38 (7–9), 125–135. https://doi.org/10.1111/j.1365-3032.1963.tb00767.x
Da Costa, J., Cerri, D., Sá, M.R. & Lamas, C.J.E. (2008) Coleção entomológica do Instituto Oswaldo Cruz: resgate de acervo científico-histórico disperso pelo Massacre de Manguinhos. História, Ciências, Saúde-Manguinhos, 15 (2), 401–410. https://doi.org/10.1590/S0104-59702008000200010
Da Costa Lima, A. (1931) Sobre as especies de Megarhinus do Brasil: (Diptera: Culicidae). Memórias do Instituto Oswaldo Cruz, 25 (4), 307–315. https://doi.org/10.1590/S0074-02761931000400002
Da Costa Lima, A.M., Guitton, N. & Ferreira, O. (1962) Comentários relativos às espécies da tribo Toxorhynchitini (Megarhinini) com a descrição de uma espécie nova de Lynchiella (Diptera, Culicidae). Memórias do Instituto Oswaldo Cruz, 60 (2), 225–251, 4 pls. https://doi.org/10.1590/S0074-02761962000200008
Da Silva, M.A.N. & Lozovei, A.L. (1999) Ocorrência de Haemagogus (Conopostegus) leucocelaenus (Dyar & Shannon) and Toxorhynchites (Lynchiella) theobaldi (Dyar & Knab) em ocos de árvore em capão de mata, Curitiba, Paraná, Brasil. Revista Brasileira de Zoologia, 16 (Supplement 1), 257–267. https://doi.org/10.1590/S0101-81751999000500018
De Deus Palma, J. & Galvao, A.B. (1969) Lynchiella rizzoi sp. n., toxorrinquitíneo bromelícola do estado de Goiás (Diptera, Culicidae). Revista Goiana de Medicina, 15 (1 & 2), 3–13.
Donald, C.L., Siriyasatien, P. & Kohl, A. (2020) Toxorhynchites species: A review of current knowledge. Insects, 11, 747. https://doi.org/10.3390/insects11110747
Dyar, H.G. (1928) The mosquitoes of the Americas. Publication No. 387. Carnegie Institute of Washington, Washington, D.C., 616 pp.
Dyar, H.G. & Knab, F. (1906) The species of mosquitoes in the genus Megarhinus. Smithsonian Miscellaneous Collections, 48 (3), 241–258.
Edwards, F.W. (1921a) H. Sauter’s Formosan collections: Culicidae. Annals and Magazine of Natural History, 8 (48), 629‒632. https://doi.org/10.1080/00222932108632632
Edwards, F.W. (1921b) Mosquito notes.‒II. Bulletin of Entomological Research, 12 (1), 69‒80. https://doi.org/10.1017/S0007485300044874
Edwards, F.W. (1932) Diptera, Family Culicidae. Genera Insectorum. Diptera, Fam. Culicidae. Fascicle 194. M.P. Wytsman, Bruxelles, 258 pp., 5 pls.
Fabricius, J.C. (1787) Mantissa Insectorum, sistens eorum species nuper detectas: adjectis synonymis, observationibus, descriptionibus, emendationibus. Vol. 2. Christ. Gottl. Proft, Hafniæ [Copenhagen], 382 pp. https://doi.org/10.5962/bhl.title.36471
Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39 (4), 783–791. https://doi.org/10.2307/2408678
Folmer, O., Black, M., Hoeh, W., Lutz R. & Vrijenhoek R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology Biotechnolology, 3 (5), 294–299.
Forattini, O.P. (1996) Culicidologia médica: identificação, biologia, epidemiologia. Vol. 2. Editora da Universidade de São Paulo, São Paulo, 549 pp.
García, M. & Casal, O.H. (1967) Toxorhynchites (Lynchiella) cavalierii, nueva especie bromelícola de la República Argentina (Diptera, Culicidae) [sic]. Physis, 26 (73), 435–441.
Godoy, R.S.M., Fernandes, K.M. & Martins, G.F. (2015) Midgut of the non-hematophagous mosquito Toxorhynchites theobaldi (Diptera, Culicidae). Scientific Reports, 5, 15836.
https://doi.org/10.1038/srep15836
Harbach, R.E. (1995) A new Sabethes of the subgenus Peytonulus (Diptera: Culicidae) with an unusual fourth-instar larva. Entomologica scandinavica, 26 (1), 87‒96.
Harbach, R.E. (2021) Mosquito taxonomic inventory. Available from: http://mosquito-taxonomic-inventory.info/simpletaxonomy/term/6272 (accessed 23 March 2021)
Harbach, R.E. & Kitching, I.J. (1998) Phylogeny and classification of the Culicidae (Diptera). Systematic Entomology, 23 (4), 327–370. https://doi.org/10.1046/j.1365-3113.1998.00072.x
Harbach, R.E. & Knight, K.L. (1980) Taxonomists’ glossary of mosquito anatomy. Plexus Publishing, Inc., Marlton, New Jersey, xi + 413 pp.
Howard, L.O., Dyar, H.G. & Knab, F. (1917) The mosquitoes of North and Central America and the West Indies. Vol. 4. Systematic description (in two oparts), Part II. Publication No. 159. Carnegie institution of Washington, Washington, D.C., pp. 525‒1064. https://doi.org/10.5962/bhl.title.54495
Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16 (2), 111–120. https://doi.org/10.1007/BF01731581
Knab, F. (1911) The food-habits of Megarhinus. Psyche: A Journal of Entomology, 18 (2), 80–82. https://doi.org/10.1155/1911/327256
Knab, F. (1913) A new bromelicolous Megarhinus (Diptera: Culicidae). Insecutor Inscitiae Menstruus, 1 (3), 35–36. https://doi.org/10.5962/bhl.part.9558
Krishnamoorthy, R., Munirathinam, A., Khananjeyan, K.J., Hiriyan, J., Mariappan, T., Samuel, P.P. & Venkatesh, A. (2013) Description of a new species, Toxorhynchites (Toxorhynchites) tyagii (Diptera: Culicidae), from Nilgiri hills, Western Ghats, southern India. Zootaxa, 3701 (4), 447–459. https://doi.org/10.11646/zootaxa.3701.4.4
Kumar, S., Stecher, G. & Tamura, K. (2016) Mega7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33 (7), 1870–1874. https://doi.org/10.1093/molbev/msw054
La Corte, R., Maia, P.C.R., Dolabella, S.S., Cruz, D.E.R. & Marteis, L.S. (2018) Mosquitoes of the Caatinga. III. Larval habitats, frequency, and dynamics of immature and adult stages in a dry Brazilian forest. Journal of Medical Entomology, 56 (1), 120–128. https://doi.org/10.1093/jme/tjy160
Lahille, F. (1904) Notes sur la classification des moustiques. Actas y Trabajos del Segundo Congreso Médico Latino-Americano, Buenos Aires, 2, 71‒96.
Lane, J. (1944) A tribo Megarhinini no Brasil Meridional. Revista de Entomologia, 15 (1‒2), 172–190.
Lane, J. (1953) Neotropical Culicidae. Vol. I. University of São Paulo, São Paulo, 548 pp.
Lopes, J. (1999) Ecologia de mosquitos (Diptera, Culicidae) em criadouros naturais e artificiais de área rural do norte do Paraná, Brasil. VIII. Influência das larvas predadoras (Toxorhynchites sp., Limatus durhamii e Culex bigoti) sobre a população de larvas de Culex Quinquefasciatus [sic] e Culex eduardoi. Revista Brasileira de Zoologia, 16 (3), 821–826. https://doi.org/10.1590/S0101-81751999000300018
Lounibos, L.P. (1979) Temporal and spatial distribution, growth and predatory behaviour of Toxorhynchites brevipalpis (Diptera: Culicidae) on the Kenya coast. Journal of Animal Ecology, 48 (1), 213–236. https://doi.org/10.2307/4110
Lutz, A. & Neiva, A. (1913) Contribuiçãos para a biolojia das megarininas com descriçãos de duas espécies novas. Memórias do Instituto Oswaldo Cruz, 5 (2), 130–141. https://doi.org/10.1590/S0074-02761913000200003
Lutz, A. & Neiva, A. (1914) Contribuição para o estudo das “Megarhininae”. II. Do “Megarhinus haemorrhoidalis” (Fabricius, 1794). Memórias do Instituto Oswaldo Cruz, 6 (1), 50–57. https://doi.org/10.1590/S0074-02761914000100007
Marteis, L.S., Natal, D., Sallum, M.A.M., Medeiros-Sousa, A.R., Oliveira, T.M.P. & La Corte, R. (2017a) Mosquitoes of the Caatinga: 1. Adults stage survey and the emerge of seven news species endemic of a dry tropical forest in Brazil. Acta Tropica, 166, 193–201. https://doi.org/10.1016/j.actatropica.2016.11.022
Marteis, L.S., Natal, D., Sallum, M.A.M., Medeiros-Sousa, A.R. & La Corte, R. (2017b) Mosquitoes of the Caatinga: 2. Species from periodic sampling of bromeliads and tree holes in a dry Brazilian forest. Acta Tropica, 171, 114–123. https://doi.org/10.1016/j.actatropica.2017.03.031
Matsumura, S. (1916) Thousand insects of Japan [Shin Nihon senchu zukai]. Additamenta [Shin Nihon senchu zukai]. Vol. II. Keisei-sha, Tokyo, pp. 185‒474, 25 pls, contents.
Mitchell, A., Sperling, F.A.H. & Hickey, D.A. (2002) Higher-level phylogeny of mosquitoes (Diptera: Culicidae): mtDNA data support a derived placement for Toxorhynchites. Insect Systematics and Evolution, 33 (2), 163–174. https://doi.org/10.1163/187631202X00118
Muspratt, J. (1951) The bionomics of an African Megarhinus (Dipt., Culicidae) and its possible use in biological control. Bulletin of Entomological Research, 42 (2), 355–370. https://doi.org/10.1017/S0007485300025372
Newkirk, M.R. (1947) Observations on Megarhinus splendens Wiedemann with reference to its value in biological control of other mosquitoes (Diptera: Culicidae). Annals of the Entomological Society of America, 40 (3), 522–527. https://doi.org/10.1093/aesa/40.3.522
Nimer, E. (1972) Climatologia da Região Nordeste do Brasil. Revista Brasileira de Geografia, 34 (2), 3–51.
Paes, M.L.N. & Dias, I.F.O. (2008) Plano de Manejo: Estação Ecológica Raso da Catarina. Instituto Chico Mendes de Conservação da Biodiversidade, Ibama, Brasília, 326 pp.
Ribeiro, H. (1992) Research on the mosquito subfamily Toxorhynchitinae (Diptera: Culicidae) II – description of the Afrotropical subgenus Afrorhynchus subg. nov. Mosquito Systematics, 23 (3), 195‒198. [for 1991]
Robineau-Desvoidy, J.-B. (1827) Essai sur la tribu des culicides. Mémoires de la Société d’Histoire Naturelle de Paris, 3, 390‒413, 1 pl.
Saitou, N. & Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4 (4), 406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
Skuse, F.A.A. (1889) Diptera of Australia. Part V.―The Culicidae. Proceedings of the Linnean Society of New South Wales, 3 (4), 1717‒1764, 1 pl.
Steffan, W.A. & Evenhuis, N.L. (1981) Biology of Toxorhynchites. Annual Review of Entomology, 26, 159–181. https://doi.org/10.1146/annurev.en.26.010181.001111
Theobald, F.V. (1901) The classification of mosquitoes. Journal of Tropical Medicine, 4, 229‒235.
Thurman, E.B. (1959) A contribution to a revision of the Culicidae of northern Thailand. University of Maryland Agricultural Experimental Station Bulletin, A-100, 1‒182.
Wiedemann, C.R.G. (1819) Beschreibung neuer Zweiflügler aus Ostindien und Afrika. Zoologisches Magazin, 1 (3), 1‒39.
Wiedemann, C.R.G. (1820) Diptera exotica. Sectio I. antennis multiarticulatis. Wiedemann, Kiliae [Kiel], xix + 38 pp.
Wilkerson, R.C., Linton, Y.-M. & Strickman, D. (2021) Mosquitoes of the world. Vols. 1 & 2. Johns Hopkins University Press, Baltimore, Maryland, 1332 pp.