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Type: Article
Published: 2021-08-05
Page range: 257-270
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Mosquitoes (Diptera: Culicidae) in Eocene amber from the Rovno region, Ukraine

University of Gdańsk, Faculty of Biology, Department of Invertebrate Zoology and Parasitology, Laboratory of Systematic Zoology; Wita Stwosza 59, 80-308 Gdańsk, Poland.
Natural History Museum, Department of Life Sciences; Cromwell Road, London SW7 5BD, UK.
I.I. Schmalhausen Institute of Zoology, National Academy of Sciences of Ukraine; B. Khmelnitskogo 15, Kiev, 01601, Ukraine. 4A.A. Borissiak Paleontological Institute, Russian Academy of Sciences; Profsoyuznaya Str. 123, Moscow, 117868 Russia.
Diptera Culex Culiseta fossils new species systematics


Culex ekaterinae sp. nov. is described based on an adult male embedded in amber from the Rovno region, northwestern Ukraine. The new species is compared with its presumed closest known relative, i.e. Culex erikae Szadziewski et Szadziewska, 1985 described from adults in Baltic amber. The two species exhibit distinct differences in the size of the main body parts, including the antenna, maxillary palpus and proboscis, as well as in the venation of the wing, the structure of the ungues and male genitalia—characters crucial in the diagnosis of adult mosquitoes. A redescription of Culiseta gedanica Szadziewski et Giłka, 2011, to date known from a single adult male, is presented based on a male in amber from Voronki, Rovno region. A tabulation of fossil Culicidae with their geological ages is provided.



  1. Andersen, T., Baranov, V., Goral, T., Langton, P., Perkovsky, E. & Sykes, D. (2015) First record of a Chironomidae pupa in amber. Geobios, 48 (4), 281–286.
    Azar, D., Nel, A. & Perkovsky, E. (2013) A new Sycorax species from Eocene Ukrainian Rovno Amber (Diptera: Psychodidae: Sycoracinae). In: Azar, D., Engel, M.S., Jarzembowski, E., Krogmann, L. & Santiago-Blay, J. (Eds.), Insect evolution in an amberiferous and stone alphabet. Proceedings of the 6th International Congress on Fossil Insects, Arthropods and Amber, Leiden, Boston, Brill, pp. 27–46.
    Baranov, V., Andersen, T. & Perkovsky, E. (2014) A new genus of Podonominae (Diptera: Chironomidae) in Late Eocene Rovno amber from Ukraine. Zootaxa, 3794, 581–586.
    Baranov, V.A., Kvifte, G.M. & Perkovsky, E.E. (2016) Two new species of fossil Corethrella Coquillett from Late Eocene Rovno amber, with a species-level phylogeny for the family based on morphological traits (Diptera: Corethrellidae). Systematic Entomology, 41 (2), 531–540.
    Baranov, V.A. & Perkovskу, E.E. (2014) First record of gynandromorphy in fossil Chironomidae (Diptera) from Late Eocene Rovno amber. Chironomus, 27, 55–57.
    Belkin, J.N. (1962) The mosquitoes of the South Pacific (Diptera, Culicidae). Vols 1 & 2. University of California Press, Berkeley and Los Angeles, xii + 608 and 412 pp.
    Blanchard, R. (1901) Observations sur quelques moustiques. Comptes Rendus Hebdomadaires des Séances et Mémoires de la Société de Biologie, 53, 1045‒1046.
    Borkent, A. & Grimaldi, D.A. (2004) The earliest fossil mosquito (Diptera: Culicidae), in mid-Cretaceous amber. Annals of the Entomological Society of America, 97 (5), 882‒888.[0882:TEFMDC]2.0.CO;2
    Cockerell, T.D.A. (1916) British fossil insects. Proceedings of the United States National Museum, 49 (2119), 469‒499, 6 pls.
    Cockerell, T.D.A. (1919) The oldest mosquitoes. Nature, 103 (2577), 43‒46.
    Dietrich, C.H. & Perkovsky, E.E. (2020) A new genus and species representing the first leafhopper (Hemiptera: Cicadellidae) from Eocene Rovno amber. Palaeoentomology, 3 (2), 180–187.
    Dyar, H.G. (1905) Remarks on genitalic genera in the Culicidae. Proceedings of the Entomological Society of Washington, 7 (1), 42‒49.
    Edwards, F.W. (1923) Oligocene mosquitoes in the British Museum; with a summary of our present knowledge concerning fossil Culicidae. Quarterly Journal of the Geological Society of London, 79 (2), 139‒155, 1 pl.
    Evenhuis, N.L. (1994) Catalogue of the fossil flies of the world (Insecta: Diptera). Backhuys Publishers, Leiden, 600 pp.
    Fedotova, Z.A. & Perkovsky, E.E. (2017) New genus and species of gall midges (Diptera, Cecidomyiidae, Porricondylinae, Holoneurini) from the Late Eocene amber of Olevsk (Zhitomir Region, Ukraine). Vestnik Zoologii, 51 (1), 23–30.
    Felt, E.P. (1904) Mosquitos or Culicidae of New York State. New York State Museum Bulletin, 79 (323), 241‒391, 57 pls, 391a‒391f + 393‒400.
    Giłka, W., Zakrzewska, M., Dominiak, P. & Urbanek, A. (2013) Non-biting midges of the tribe Tanytarsini in Eocene amber from the Rovno region (Ukraine): a pioneer systematic study with notes on the phylogeny (Diptera: Chironomidae). Zootaxa, 3736 (5), 569–586.
    Harbach, R.E. (2021) Mosquito Taxonomic Inventory. (accessed 29 April 2021).
    Harbach, R. & Greenwalt, D. (2012) Two Eocene species of Culiseta (Diptera: Culicidae) from the Kishenehn Formation in Montana. Zootaxa, 3530 (1), 25–34.
    Harbach, R.E. & Knight, K.L. (1980) Taxonomists’ glossary of mosquito anatomy. Plexus Publishing, Marlton, NJ, xi + 415 pp.
    Harbach, R.E. & Knight, K.L. (1982) Corrections and additions to Taxonomists’ Glossary of Mosquito Anatomy. Mosquito Systematics, 13 (2), 201–217. [for 1981]
    Kopeć, K., Perkovsky, E. & Skibińska, K. (2019) A new species of a genus Cheilotrichia (Diptera: Limoniidae) from Baltic and Ukrainian amber. Annales Zoologici, 69 (2), 423–426.
    Krzemiński, W., Blagoderov, V., Azar, D., Lukashevich, E., Szadziewski, R., Wedmann, S., Nel, A., Collomb, F.-M., Waller, A. & Nicholson, D. (2019) True flies (Insecta: Diptera) from the late Eocene insect limestone (Bembridge Marls) of the Isle of Wight, England, UK. Earth and Environmental Science Transactions of the Royal Society of Edinburgh, 110 (3–4), 495–554.
    Linnaeus, C. (1758) Systema naturae per regna tria naturae, secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis, locis. Tomus 1, Editio Decima. Impensis Direct, Laurentii Salvii, Holmiae, 824 pp.
    Lynch Arribálzaga, F. (1891) Dipterologia argentine. Revista del Museo de La Plata, 1, 345‒377.
    Lyubarsky, G.Y. & Perkovsky, E.E. (2020) First Rovno amber species of the genus Telmatophilus (Coleoptera: Clavicornia: Cryptophagidae) from Veselukha floodplain. Invertebrate Zoology, 17 (1), 25–35.
    Meigen, J.W. (1818) Systematische Beschreibung der bekannten europäischen zweiflügeligen Insekten. Vol. 1. Aachen, xxxvi + 332 pp., errata (1 p.), 11 pls.
    Meunier, F. (1915) Nouvelles recherches sur quelques insectes des plâtrières d’Aix en Provence. Verhandelingen der Koninklijke Akademie van Wetenschappen le Amsterdam, Tweede Sectie, 18 (5), 1‒17, 5 pls.
    Mitov, P.G., Perkovsky, E.E. & Dunlop, J.A. (2021) Harvestmen (Arachnida: Opiliones) in Eocene Rovno amber (Ukraine). Zootaxa, 4984 (1), 43–72.
    Perkovsky, E.E. (2013) Eohelea sinuosa (Meunier, 1904) (Diptera, Ceratopogonidae) in Late Eocene Ambers of Europe. Paleontological Journal, 47 (5), 503–512.
    Perkovsky, E.E. (2017) Comparison of biting midges of the Early Eocene Cambay amber (India) and Late Eocene European ambers supports the independent origin of European ambers. Vestnik Zoologii, 51 (4), 275–284.
    Perkovsky, E.E. & Fedotova, Z.A. (2016) Rovnodiplosis eduardi gen. et sp. nov., the first record of a fossil gall midge of the supertribe Mycodiplosidi (Diptera, Cecidomyioidea, Cecidomyiidae) in the Late Eocene of the Rovno amber. Paleontological Journal, 50 (9), 1027–1032.
    Perkovsky, E.E. & Nel, A. (2021) New Rovno amber termite genus (Isoptera, Rhinotermitidae) from Styr River basin. Palaeontologia Electronica, 24 (1), a05.
    Perkovsky, E.E., Olmi, M., Vasilenko, D.V., Capradossi, L. & Guglielmino, A. (2020) First Bocchus Ashmead (Hymenoptera: Dryinidae) from Upper Eocene Rovno amber: B. schmalhauseni sp. nov. Zootaxa, 4819 (3), 544–556.
    Perkovsky, E.E. & Rasnitsyn, A.P. (2013) Biting midges (Diptera, Ceratopogonidae) in amber forest communities based on analysis of syninclusions in Late Eocene Rovno amber. Terrestrial Arthropod Reviews, 6, 71–80.
    Perkovsky, E.E. & Sukhomlin, E.B. (2015) New Late Eocene blackflies (Diptera, Simuliidae) from the Rovno Amber (Ukraine). Paleontological Journal, 49 (6), 608–614.
    Perkovsky, E.E. & Sukhomlin, E.B. (2016) A new species of Hellichiella (Diptera: Simuliidae) with 11-segmented antenna from the Eocene. Israel Journal of Entomology, 46, 79–86.
    Perkovsky, E.E., Sukhomlin, E.B. & Panchenko, А.А. (2013) The modern distribution of blackflies genera from Baltic and Rovno amber. In: Proceedings, VIII Congress of the Ukrainian Entomological Society, Kiev, 26–30 August 2013, pp. 121–122.
    Perkovsky, E.E., Zosimovich, V.Y. & Vlaskin, A.P. (2010) Rovno amber. In: Penney, D. (ed.) Biodiversity of fossils in amber from the major world deposits. Siri Scientific Press, Manchester, pp. 116–136.
    Pielowska, A., Sontag, E. & Szadziewski, R. (2018) Haematophagous arthropods in Baltic amber. Annales Zoologici, 68 (2), 237–249.
    Piton, L. (1936) Addition a la faune entomologique des cinérites du Lac Chambon (Puy-de-Dome). Revue Scientifique du Bourbonnais, 1936, 16‒24.
    Poinar, G. Jr. (2005) Culex malariager, n. sp. (Diptera: Culicidae) from Dominican amber: the first fossil mosquito vector of Plasmodium. Proceedings of the Entomological Society of Washington, 107 (3), 548‒553.
    Poinar, G., Zavortink, T.J. & Brown, A. (2020) Priscoculex burmanicus n. gen. et sp. (Diptera: Culicidae: Anophelinae) from mid-Cretaceous Myanmar amber. Historical Biology, 32 (9), 1157–1162.
    Poinar, G.O. Jr., Zavortink, T.J., Pike, T. & Johnston, P.A. (2000) Paleoculicis minutus (Diptera: Culicidae) n. gen., n. sp., from Cretaceous Canadian amber, with a summary of described fossil mosquitoes. Acta Geologica Hispanica, 35 (1–2), 119‒128.
    Radchenko, A.G. & Perkovsky, E.E. (2021) Wheeler’s dilemma revisited: first Oecophylla –Lasius syninclusion and other ants syninclusions in the Bitterfeld amber (late Eocene). Invertebrate Zoology, 18 (1), 47–65.
    Radchenko, A.G., Perkovsky, E.E. & Vasilenko, D.V. (2021) Formica species (Hymenoptera, Formicidae, Formicinae) in late Eocene Rovno amber. Journal of Hymenoptera Research, 82, 237–251.
    Scudder, S.H. (1890) The tertiary insects of North America. United States Geological Survey of the Territories. Vol. 13. Government Printing Office, Washington, D.C., 734 pp.
    Skartveit, J. (2021) A new fossil species of the genus Bibio, with an update on bibionid flies from Baltic and Rovno amber (Diptera, Bibionidae). Deutsche Entomologische Zeitschrift, 68 (1), 81–99.
    Sokoloff, D.D., Ignatov, M.S., Remizowa, M.V., Nuraliev, M.S., Blagoderov, V., Garbout, A. & Perkovsky, E.E. (2018) Staminate flower of Prunus s. l. (Rosaceae) from Eocene Rovno amber (Ukraine). Journal of Plant Research, 131 (6), 925–943.
    Sontag, E. & Szadziewski, R. (2011) Biting midges (Diptera: Ceratopogonidae) in Eocene Baltic amber from the Rovno region (Ukraine). Polish Journal of Entomology, 80 (4), 779–800.
    Statz, G. (1944) Neue Dipteren (Nematocera) aus dem Oberoligocän von Rott. II. Teil. V. Familie Culicidae (Stechmücken). Palaeontographica, 95 (A), 108‒120, 6 pls.
    Szadziewski, R. (1998) New mosquitoes from Baltic amber (Diptera: Culicidae). Polskie Pismo Entomologiczne, 67 (3–4), 233‒244.
    Szadziewski, R. & Giłka, W. (2011) A new fossil mosquito, with notes on the morphology and taxonomy of other species reported from Eocene Baltic amber (Diptera: Culicidae). Polish Journal of Entomology, 80 (4), 765–777.
    Szadziewski, R., Sontag, E. & Szwedo, J. (2019) Mosquitoes of the extant avian malaria vector Coquillettidia Dyar, 1905 from Eocene Baltic amber (Diptera: Culicidae). Palaeoentomology, 2 (6), 650–656.
    Szadziewski, R. & Szadziewska, M. (1985) New mosquitoes from Baltic amber (Diptera: Culicidae). Polish Journal of Entomology, 55, 513–518.
    Theobald, F.V. (1901) The classification of mosquitoes. Journal of Tropical Medicine, 4, 229‒235.
    von Heyden, L. (1870) Fossile Dipteren aus der Braunkohle van Rott im Siebengebirge. Palaeontographica, 17 (6), 237‒266, 2 pls.
    Wagner, R. (2021) Neoarisemus groehni sp. nov., a notable moth fly (Diptera, Psychodidae, Psychodinae) from Ukrainian amber. Palaeoentomology, 4 (1), 19–22.
    Wojtoń, M., Kania, I. & Krzemiński, W. (2019) Review of Mycetobia Meigen, 1818 (Diptera, Anisopodidae) in the Eocene ambers. Zootaxa, 4544 (1), 1–40.
    Zakrzewska, M. & Giłka, W. (2014) The oldest known chironomids of the tribe Tanytarsini (Diptera: Chironomidae) indicate plesiomorphic character states. Geobios, 47, 335–343.
    Zakrzewska, M., Krzemiński, W. & Giłka, W. (2016) Towards the diversity of non-biting midges of the tribe Tanytarsini from Eocene Baltic amber (Diptera: Chironomidae). Palaeontologia Electronica, 19.2.18A, 1–21.
    Zavortink, T.J. & Poinar, G.O. Jr. (2000) Anopheles (Nyssorhynchus) dominicanus sp. n. (Diptera: Culicidae) from Dominican Amber. Annals of the Entomological Society of America, 93 (6), 1230‒1235.[1230:ANDSND]2.0.CO;2
    Zavortink, T.J. & Poinar, G.O. Jr. (2008) Toxorhynchites (Toxorhynchites) mexicanus, n. sp. (Diptera: Culicidae) from Mexican amber: a New World species with Old World affinities. Proceedings of the Entomological Society of Washington, 110 (1), 116‒125.
    Zelentsov, N.I., Baranov, V.A., Perkovsky, E.E. & Shobanov, N.A. (2012) First records on non-biting midges (Diptera: Chironomidae) from the Rovno amber. Russian Entomological Journal, 21 (1), 79–87.