Abstract
Chironomids of the genus Pagastia Oliver (Diamesinae, Diamesini) from the mountains of Central Asia are revised using both morphological characters and molecular data. Illustrated descriptions of the adult male Pagastia (P.) caelestomontana sp. nov. from Kirgizstan and Tajikistan, P. (P.) hanseni sp. nov. from Tajikistan, and record of a finding apparently a new species P. (P.) aff. lanceolata (Tokunaga) from Tajikistan as well as an updated a key to the determination of the adult males of all known species of Pagastia are provided. A phylogenetic framework is reconstructed based on two mitochondrial genes cytochrome oxidase subunit I (COI) sequences of 34 samples belonging to 7 species of the genus Pagastia and cytochrome oxidase subunit II (COII) available for most samples. Phylogenetic trees of some known species of the genus Pagastia were reconstructed using the combined dataset and Bayesian inference (BI) and Maximum Likelihood (ML) methods. The interspecific K2P distances between seven Pagastia species including P. (P.) caelestomontana sp. nov., P. (P.) hanseni sp. nov. and undescribed P. (P.) aff. lanceolata (Tokunaga) are 6.3–13.2 which corresponding to species level.
References
Ashe, P. & O’Connor, J.P. (2009) A World Catalogue of Chironomidae (Diptera). Part 1. Buchonomyiinae, Chilenomyiinae, Podonominae, Aphroteniinae, Tanypodinae, Usambaromyiinae, Diamesinae, Prodiamesinae and Telmatogetoninae. Irish Biogeographical Society & National Museum of Ireland, Dublin, 445 pp.
Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32, 792–1797.
https://doi.org/10.1093/nar/gkh340
Ekrem, T., Willassen, E. & Stur E. (2010) Phylogenetic utility of five genes for dipteran phylogeny: a test case in the Chironomidae leads to generic synonymies. Molecular phylogenetics and evolution, 57, 561–571.
https://doi.org/10.1016/j.ympev.2010.06.006
Endo, K. (2004) Genus Pagastia Oliver (Diptera: Chironomidae) from Japan, with description of a new species. Entomological Science, 7, 277–289.
https://doi.org/10.1111/j.1479-8298.2004.00074.x
Felsenstein, J. (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. Journal of Molecular Evolution, 17, 368–376.
https://doi.org/10.1007/BF01734359
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299
Hansen, D.C. & Cook, E.F. (1976) The systematics and morphology of the Nearctic species of Diamesa Meigen, 1835 (Diptera: Chironomidae). Memoirs of the American Entomological Society, 30, 1–203.
Hebert, P.D. N., Ratnasingham, S., Zakharov, E.V., Telfer A.C., Levesque-Beaudin, V., Milton, M.A., Pedersen, S., Jannetta, P. & deWaard, J.R. (2016) Counting animal species with DNA barcodes: Canadian insects Philosophical Transactions of The Royal Society B Biological Sciences, B3712015033320150333.
https://doi.org/10.1098/rstb.2015.0333
Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33 (7), 1870–1874.
https://doi.org/10.1093/molbev/msw054
Kustareva, L.A. & Ivanova, L.M. (1980) Benthos of Issyk-Kul Lake tributaries, Frunze, Ilim, 103 p. [in Russian]
Lanfear, R., Calcott, B., Ho, S.Y. & Guindon, S. (2012) Partitionfinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution, 29 (6), 1695–1701.
https://doi.org/10.1093/molbev/mss020
Makarchenko, E.A. (1985) Chironomids of the Soviet Far East. Subfamilies Podonominae, Diamesinae and Prodiamesinae (Diptera, Chironomidae). DVNC AN SSSR Press, Vladivostok, 208 pp. [in Russian]
Makarchenko, E.A. (1994) Chironomids of the Diamesinae (Diptera, Chironomidae) from Japan. IV. Pagastia Oliver, 1959. Japanese Journal of Entomology, 62, 823–837.
Makarchenko, E.A., Kerkis, I.E. & Ivanchenko, O.V. (1997) Morphokaryological description of Pagastia altaica sp. n. (Diptera, Chironomidae) from Altai Mountains, with the key to Holarctic species of Pagastia Oliver. Far Eastern Entomologist, 43, 1–8.
Makarchenko, E.A. & Makarchenko, M.A. (2000) Revision of Pagastia Oliver, 1959 (Diptera, Chironomidae) of the Holarctic region. In: Hoffrichter, O. (Ed.), Late 20th Century Research on Chironomidae: an Anthology from the 13th International Symposium on Chironomidae. Shaker Verlag, Achen, pp. 171–176.
Makarchenko, E.A. (2006) 3. Subfamily Diamesinae. In: Lelej, A. (Ed.), Key to the insects of Russian Far East. Vol. 6. Diptera and Siphonaptera. Pt 4. Dal’nauka, Vladivostok, pp. 253–276, 468–480, 607–621. [in Russian]
Makarchenko, E.A. & Wang, X. (2017) Pagastia tianmumontana sp. n. – a new species of chironomids (Diptera: Chironomidae: Diamesinae) from South China. Far Eastern Entomologist, 336, 13–15.
Makarchenko, E.A. (2019) Review of the genus Pagastia Oliver (Diptera: Chironomidae: Diamesinae) from North America, with description of P. (P.) subletteorum sp. nov. Zootaxa, 4664 (1),115–128.
https://doi.org/10.11646/zootaxa.4664.1.5
Makarchenko, E.A. & Hansen, D.C. (2020) Pagastia (P.) donoliveri sp. nov. – a new Nearctic alpine stream chironomid species (Diptera: Chironomidae: Diamesinae) from the Beartooth Mountains, Wyoming, U.S.A. Zootaxa, 4755 (1), 171–176. https://doi.org/10.11646/zootaxa.4755.1.9
Montagna, M., Mereghetti, V., Lencioni, V. & Rossaro, B. (2016) Integrated Taxonomy and DNA Barcoding of Alpine Midges (Diptera: Chironomidae). PLoS ONE, 11 (3), e0149673.
https://doi.org/10.1371/journal.pone.0149673
Moubayed, J. & Langton, P.H. (2019) Chaetocladius berythensis sp.n ., C. callauensis sp.n ., C. guardiolei sp.n. and C. parerai sp.n., four relict species inhabiting glacial springs and streams in Eastern Pyrenees and Lebanon (Diptera: Chironomidae). CHIRONOMUS Journal of Chironomidae Research, 32, 42–59.
https://doi.org/10.5324/cjcr.v0i32.3000
Oliver, D.R. (1959) Some Diamesini (Chironomidae) from the Nearctic and Palaearctic. Entomologisk Tidskrift, 80, 48–64.
Oliver, D.R. (1983) The larvae of Diamesinae (Diptera: Chironomidae) of the Holarctic region – Keys and diagnoses. Entomologica Scandinavica, Supplement, 19, 115–138.
Oliver, D.R. (1986) The pupae of Diamesinae (Diptera: Chironomidae) of the Holarctic region – Keys and diagnoses. Entomologica Scandinavica, Supplement, 28, 119–137.
Oliver, D.R. (1989) The adult males of Diamesinae (Diptera: Chironomidae) of the Holarctic region – Keys and diagnoses. Entomologica Scandinavica, Supplement, 34, 129–154.
Oliver, D.R. & Roussel, M.E. (1982) The larvae of Pagastia Oliver (Diptera: Chironomidae) with descriptions of three Nearctic species. The Canadian Entomologist, 114, 849–854.
https://doi.org/10.4039/Ent114849-9
Puillandre, N., Lambert, A., Brouillet, S. & Achaz, G. (2012) ABGD, Automatic Barcode Gap Discovery for primary species delimitation. Molecular Ecology, 21 (8), 1864–1877.
https://doi.org/10.1111/j.1365-294X.2011.05239.x
Rambaut A, Drummond A.J., Xie D., Baele G. & Suchard M.A. (2018) Posterior summarisation in Bayesian phylogenetics using Tracer 1.7. Systematic Biology, 67 (5), 901–904.
https://doi.org/10.1093/sysbio/syy032
Roback, S.S. & Coffman, W.P. (1987) Results of the Nepal Alpine Zone research project, Chironomidae (Diptera). Proceedings of the Academy of Natural Science of Philadelphia, 139, 87–158.
Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.
https://doi.org/10.1093/bioinformatics/btg180
Ronquist, F., Teslenko, M., Mark, P.V.D., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space. Systematic Biology, 61, 539–542.
https://doi.org/10.1093/sysbio/sys029
†Sæther, O.A. (1980) Glossary of chironomid morphology terminology (Chironomidae, Diptera). Entomologica scandinavica, Supplement 14, 1–51.
†Sæther, O.A. & Andersen, T. (2003) 7. The larvae of Diamesinae (Diptera: Chironomidae) from the Holarctic Region – Keys and diagnoses. In: Andersen, T., Cranston, P.S. & Epler, J.H. (Eds.), The larvae of Chironomidae (Diptera) of the Holarctic Region – Keys and diagnoses. Insect Systematics & Evolution, Supplement, 66, 145–178.
Serra-Tosio, B. (1971) Contribution á l’étude taxonomique, phylogénétique, biogeographique et écologique des Diamesini (Diptera, Chironomidae) d’Europe. Thése a l’Université scientifique et Médicale de Grenoble, 1–2, 1–462.
Serra-Tosio, B. (1976) Chironomides des Alpes: le genre Pseudodiamesa (Diptera, Chironomidae). Travaux Scientfiques du Park National de la Vanoise, 7, 117–138.
Song, C., Lin, X.-L., Wang, Q. & Wang, X.-H. (2018) DNA barcodes successfully delimit morphospecies in a superdiverse insect genus. Zoologica Scripta, 47 (3), 311–324.
https://doi.org/10.1111/zsc.12284
Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22, 2688–2690.
https://doi.org/10.1093/bioinformatics/btl446
Sublette, J.E. (1967) Type specimens of Chironomidae (Diptera) in the Canadian National Collections, Ottawa. Journal of the Kansas Entomological Society, 40 (3), 290–331.
Tavaré, S. (1986) Some Probabilistic and Statistical Problems in the Analysis of DNA Sequences. Lectures on Mathematics in the Life Sciences. American Mathematical Society, 17, 57–86.