Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2021-04-06
Page range: 391–400
Abstract views: 163
PDF downloaded: 19

Contribution to the molecular phylogeny of Sciaridae (Diptera) with special attention to the genera Bradysia and Corynoptera

Department of Ecology, University of Innsbruck, Technikerstrasse 25, 6020 Innsbruck, Austria.
Department of Ecology, University of Innsbruck, Technikerstrasse 25, 6020 Innsbruck, Austria.
Stückenberg 58, 24226 Heikendorf, Germany.
Department of Ecology, University of Innsbruck, Technikerstrasse 25, 6020 Innsbruck, Austria.
Department of Ecology, University of Innsbruck, Technikerstrasse 25, 6020 Innsbruck, Austria.
Department of Ecology, University of Innsbruck, Technikerstrasse 25, 6020 Innsbruck, Austria. Institute for Alpine Environment, Eurac Research, Viale Druso 1, 39100 Bozen/Bolzano, Italy
Diptera 16S 28S Alpinosciara gen. n. black fungus gnats CO1 molecular markers

Abstract

The systematics of the dipteran family Sciaridae is based mainly on morphological characters and has remained quite controversial. In this study, we used two mitochondrial DNA markers (CO1, 16S) and a nuclear one (28S) to take a glimpse into phylogenetic relationships of part of the North and Central European Sciaridae. A total of 91 species from 19 genera were analysed using Maximum Likelihood based phylogenetics (depending on the availability of valid sequences, 50–70 per gene). We strengthen the suggestion of the Chaetosciara group as an independent subfamily. Within the subfamily Megalosphyinae, two separate Bradysia clades were identified, suggesting a close relation between the genera Zygoneura, Austrosciara, and Scatopsciara. The genus Alpinosciara gen. n. is established to place the species of the former Corynoptera crassistylata group inside the subfamily Megalosphyinae. This new genus now includes 22 species.

 

References

  1. Deady, R., Heller, K., Work, T. & Venier, L. (2014) Peyerimhoffia jaschhoforum (Diptera, Sciaridae), a new deadwood inhabiting species from Canada. Biodiversity Data Journal, 2, e4200.

    https://doi.org/10.3897/BDJ.2.e4200

    Degnan, J.H. & Rosenberg, N.A. (2009) Gene tree discordance, phylogenetic inference and the multispecies coalescent. Trends in Ecology & Evolution, 24, 332–340.

    https://doi.org/10.1016/j.tree.2009.01.009

    Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.

    Frank, J., Menzel, F. & Dettner, K. (2005) Black fungus gnats (Diptera: Sciaridae) collected at cantharidin baits in southern and central Europe. Studia Dipterologica, 11, 359–368.

    Frey, R. (1942) Entwurf einer neuen Klassifikation der Mückenfamilie Sciaridae. Notulae Entomologicae, 22, 5–44.

    Heller, K. (2019a) Trichosiopsis—Species-ID. Available from: https://species-id.net/w/index.php?title=Trichosiopsis&oldid=180652 (accessed 5 November 2019)

    Heller, K. (2019b) Hirtipennia—Species-ID. Available from: https://species-id.net/w/index.php?title=Hirtipennia&oldid=180711 (accessed 5 November 2019)

    Heller, K. (2019c) Leptospina—Species-ID. Available from: https://species-id.net/w/index.php?title=Leptospina&oldid=179458 (accessed 5 November 2019)

    Heller, K. & Menzel, F. (2013) s.n. In: Sciaridae, P.L., Beuk, T. & Pape, T. (Eds.), Fauna Europaea: Diptera: Nematocera. Fauna Europaea. Version 2.6.2. [software]

    Hillis, D.M. & Bull, J.J. (1993) An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology, 42, 182–192.

    https://doi.org/10.1093/sysbio/42.2.182

    Hippa, H., Vilkamaa, P. & Heller, K. (2010) Review of the Holarctic Corynoptera Winnertz, 1867, s.str. (Diptera, Sciaridae). Zootaxa, 2659 (1), 1–197.

    https://doi.org/10.11646/zootaxa.2695.1.1

    Lorenz, R., Bernhart, S.H., Höner zu Siederdissen, C., Tafer, H., Flamm, C., Stadler, P.F. & Hofacker, I.L. (2011) ViennaRNA Package 2.0. Algorithms for Molecular Biology, 6, 26–39.

    https://doi.org/10.1186/1748-7188-6-26

    Menzel, F., Heller, K. & Köhler, A. (2011) Neue Trauermücken-Funde aus den Niederlanden (Diptera: Sciaroidea: Sciaridae). Studia Dipterologica, 17, 194–198.

    Menzel, F. & Mohrig, W. (2000) A revision of the Palaearctic black fungus gnats (Diptera: Sciaridae). Studia Dipterologica Supplement, 6, 1–761.

    Mohrig, W. (1985) Neue Trauermücken aus den Ostalpen (Insecta: Diptera, Sciaridae). Berichte des Naturwissenschaftlich-Medizinischen Vereins in Innsbruck, 72, 231–240.

    Mohrig, W., Heller, K., Hippa, H., Vilkamaa, P. & Menzel, F. (2013) Revision of the Black Fungus Gnats (Diptera: Sciaridae) of North America. Studia Dipterologica, 19, 141–286.

    Mohrig, W. & Menzel, F. (2014) Revision der neotropischen Trauermücken—Teil I. Die Gattungen Cratyna Winnertz, Euricrium Enderlein, Metangela Rübsaamen, Pseudosciara Schiner und Sciara Meigen (Diptera: Sciaridae). Contributions to Entomology, 64, 135–190.

    https://doi.org/10.21248/contrib.entomol.64.1.135-190

    Palumbi, S., Martin, A., Romano, S., McMillan, W., Stice, L. & Grabowski, G. (1991) The Simple Fool´s Guide to PCR. Available from: http://palumbi.stanford.edu/SimpleFoolsMaster.pdf (accessed 1 March 2016)

    R Development Core Team (2016) R: A Language and Environment for Statistical Computing. Version 3.3.2 R Foundation for Statistical Computing, Vienna. [program]

    Rokas, A., Nylander, J.A.A., Ronquist, F. & Stone, G.N. (2002) A maximum-likelihood analysis of eight phylogenetic markers in gallwasps (Hymenoptera: Cynipidae): Implications for insect phylogenetic studies. Molecular Phylogenetics and Evolution, 22, 206–219.

    https://doi.org/10.1006/mpev.2001.1032

    Schlick-Steiner, B.C., Steiner, F.M., Seifert, B., Stauffer, C., Christian, E. & Crozier, R.H. (2010) Integrative Taxonomy: A Multisource Approach to Exploring Biodiversity. Annual Review of Entomology, 55, 421–438.

    https://doi.org/10.1146/annurev-ento-112408-085432

    Seibel, P.N., Müller, T., Dandekar, T., Schultz, J. & Wolf, M. (2008) 4SALE—A tool for synchronous RNA sequence and secondary structure alignment and editing. BMC Bioinformatics, 7, 498–504.

    https://doi.org/10.1186/1471-2105-7-498

    Sevcik, J., Kasprak, D., Mantic, M., Fitzgerald, S., Sevcikov, T., Tothova, A. & Jaschhof, M. (2016) Molecular phylogeny of the megadiverse insect infraorder Bibionomorpha sensu lato (Diptera). PeerJ, 4, e2563.

    https://doi.org/10.7717/peerj.2563

    Shi, K., Huang, J., Zhang, S.-J. & Wu, H. (2014) Taxonomy of the genus Peyerimhoffia Kieffer from Mainland China, with a description of seven new species (Diptera, Sciaridae). ZooKeys, 382, 67–83.

    https://doi.org/10.3897/zookeys.382.4948

    Shin, S., Jung, S., Menzel, F., Heller, K., Lee, H. & Lee, S. (2013) Molecular phylogeny of black fungus gnats (Diptera: Sciaroidea: Sciaridae) and the evolution of larval habitats. Molecular Phylogenetics and Evolution, 66, 833–846.

    https://doi.org/10.1016/j.ympev.2012.11.008

    Shin, S., Lee, H. & Lee, S. (2019) Proposal of a new subfamily of Sciaridae (Diptera: Sciaridae), with description of one new species from South Korea. Zootaxa, 4543 (1), 127–136.

    https://doi.org/10.11646/zootaxa.4543.1.8

    Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.

    https://doi.org/10.1093/molbev/mst197

    Toews, D.P.L. & Brelsford, A. (2012) The biogeography of mitochondrial and nuclear discordance in animals. Molecular Ecology, 21, 3907–3930.

    https://doi.org/10.1111/j.1365-294X.2012.05664.x

    Trifinopoulos, J., Nguyen, L.-T., von Haeseler, A. & Minh, B.Q. (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research, 44, W232–W235.

    https://doi.org/10.1093/nar/gkw256

    Tuomikoski, R. (1960) Zur Kenntnis der Sciaridaen (Dipt.) Finnlands. Annales Zoologici Societatis Zoologicae Botanicae Fennicae ‘Vanamo’, 21, 1–164.

    Vilkamaa, P. & Hippa, H. (2004) The genus Xenosciara gen. n. and the phylogeny of the Sciaridae (Diptera). Zootaxa, 699 (1), 1–24.

    https://doi.org/10.11646/zootaxa.699.1.1

    Vilkamaa, P. & Hippa, H. (2005) Phylogeny of Peyerimhoffia Kieffer, with the revision of the species (Diptera : Sciaridae). Insect Systematics & Evolution, 35, 457–480.

    https://doi.org/10.1163/187631204788912445

    Vilkamaa, P., Rudzinski, H.-G., Burdikova, N. & Sevcik, J. (2018) Phylogenetic position of Aerumnosa Mohrig (Diptera, Sciaridae) as revealed by multigene analysis, with the description of four new Oriental species. Zootaxa, 4399 (2), 248–260.

    https://doi.org/10.11646/zootaxa.4399.2.8