Skip to main content Skip to main navigation menu Skip to site footer
Article
Published: 2021-03-29

Nothobranchius nikiforovi, a new species of seasonal killifish from the lower Matandu drainage in south-eastern coastal Tanzania (Cyprinodontiformes: Nothobranchiidae)

30, Rue du Mont Ussy, 77300 Fontainebleau, France.
6141 Parkwood Drive, Nanaimo, British Columbia V9T 6A2, Canada.
Department of Vertebrate Zoology, Moscow State University, 119991 Moscow, Russia.
Acari Adiniops subgenus Coastal East Africa ecoregion molecular phylogeny Nothobranchius eggersi taxonomy

Abstract

Nothobranchius nikiforovi, a new species known from seasonal habitats in the lower Matandu drainage in south-eastern coastal Tanzania is described. It is distinguished from all other congeners, except N. eggersi, by males presenting two colour phenotypes: the blue phenotype having a bright iridescent light blue to blue-green body, with narrow red-brown scale margins creating irregular reticulated pattern, forming chevron-shaped crossbars on the posteroventral portion of body and light blue median fins with red-brown dotted pattern; the red phenotype has a dark red head, light blue dorsal and anal fins proximally and medially, dark red distally, with dark red stripes parallel to the fin rays, and a plain dark red caudal fin. Nothobranchius nikiforovi differs from N. eggersi by male colour pattern, the blue phenotypes having median fins with dark grey distal portion, some of the rays of dorsal and anal fins with white tips (vs. median fins with distinct slender white distal band), and the caudal fin lacking a spotted pattern (vs. dots arranged into irregular curved stripes); the red phenotype with golden stripe between the nape and dorsal-fin origin (vs. light-blue stripe), the dorsal and anal fins with a plain red distal portion and lacking a light distal band (vs. with distinct narrow white distal band), the pelvic fin lacking a distal band (vs. with distinct slender light blue to white distal band), and some morphometric differences. Phylogenetic analyses also support the genetic distinction of the new species from its closest known relative, N. eggersi, and confirm its position in the N. guentheri species group within the Adiniops subgenus.

 

References

  1. Abell, R., Thieme, M.L., Revenga, C., Bryer, M., Kottelat, M., Bogutskaya, N., Coad, B., Mandrak, N., Contreras-Balderas, S., Bussing, W., Stiassny, M. L. J., Skelton, P., Allen, G.R., Unmack, P., Naseka, A., Ng, R., Sindorf, N., Robertson, J., Armijo, E., Higgins, J.V., Heibel, T.J., Wikramanayake, E., Olson, D., Loìpez, H.L., Reis, R.E., Lundberg, J.G., Sabaj Peìrez, M.H. & Petry, P. (2008) Freshwater ecoregions of the world: a new map of biogeographic units for freshwater biodiversity conservation. BioScience, 58 (5), 403–414.

    https://doi.org/10.1641/B580507

    Bartáková, V., Nagy, B., Polačik, M., Blažek, R., Lamtane, H. & Reichard, M. (2020) Genetic diversity of a widespread annual killifish from coastal Tanzania. BMC Evolutionary Biology, 20 (1), 1–13.

    https://doi.org/10.1186/s12862-019-1549-2

    Baur, H. & Leuenberger, C. (2011) Analysis of ratios in multivariate morphometry. Systematic Biology, 60 (6), 813–825.

    https://doi.org/10.1093/sysbio/syr061

    Cellerino, A., Valenzano, D.R. & Reichard, M. (2016) From the bush to the bench: the annual Nothobranchius fishes as a new model system in biology. Biological Reviews, 91 (2), 511–533.

    https://doi.org/10.1111/brv.12183

    Chambers, J. (1984) A new Nothobranchius species (Teleosti, Cyprinodontiformes, Aplocheilidae) from the Sudan. Journal of Fish Biology, 25 (5), 505–513.

    Cotterill, F.P.D., Nagy, B., Watters, B.R. & Bellstedt, D.U. (2016) Tracking Quaternary and Neogene landscape evolution through the geoecodynamics of fishes: unique proxy of tenures and mode of Africa’s dambos and depocentres. Quaternary International, 404, 210.

    Drummond, A.J. & Rambaut, A. (2007) BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evolutionary Biology, 7, 214.

    https://doi.org/10.1186/1471-2148-7-214

    Dytham, C. (2011) Choosing and using statistics: a biologist’s guide. 3rd Edition. Wiley-Blackwell, Oxford, Chichester & Hoboken, 320 pp.

    Gernhard, T. (2008) The conditioned reconstructed process. Journal of Theoretical Biology, 253 (4), 769–778.

    https://doi.org/10.1016/j.jtbi.2008.04.005

    Hall T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.

    Hughes, R.H. & Hughes, J.S. (1992) A directory of African wetlands. IUCN, Gland and Cambridge and UNEP, Nairobi, 820 pp.

    IUCN (2012) IUCN Red List Categories and Criteria: Version 3.1. 2nd Edition. IUCN, Gland and Cambridge, 32 pp.

    James, F.C. & McCulloch, C.E. (1990) Multivariate analysis in ecology and systematics: panacea or Pandora’s box? Annual Review of Ecology and Systematics, 21, 129–166.

    https://doi.org/10.1146/annurev.es.21.110190.001021

    Jubb, R.A. (1971) A new Nothobranchius (Pisces, Cyprinodontidae) from southeastern Rhodesia. Journal of the American Killifish Association, 8 (1), 12–19.

    Jubb, R.A. (1981) Nothobranchius. T.F.H., Neptune City, 61 pp.

    Kalyaanamoorthy, S., Minh, B., Wong, T., von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods, 14, 587–589.

    https://doi.org/10.1038/nmeth.4285

    Li, C., Orti, G., Zhang, G. & Lu, G. (2007) A practical approach to phylogenomics: the phylogeny of ray-finned fish (Actinopterygii) as a case study. BMC Evolutionary Biology, 7, 44.

    https://doi.org/10.1186/1471-2148-7-44

    Mayden, R.L. (1999) Consilience and a hierarchy of species concepts: advances toward closure on the species puzzle. Journal of Nematology, 31 (2), 95–116.

    Mayden, R.L. (2002) On biological species, species concepts and individuation in the natural world. Fish and Fisheries, 3 (3), 171–196.

    https://doi.org/10.1046/j.1467-2979.2002.00086.x

    McDonald, J.H. (2008) Handbook of biological statistics. Sparky House, Baltimore, Maryland, 287 pp.

    Minh, B.Q., Nguyen, M.A.T. & von Haeseler, A. (2013) Ultrafast approximation for phylogenetic bootstrap. Molecular Biology and Evolution, 30, 1188–1195.

    https://doi.org/10.1093/molbev/mst024

    Minitab. (2010) [Statistical computer software]. Version 16.2.1. Minitab, Inc., State College, Pennsylvania. [program]

    Mpanda, S. (1997) Geological development of the East African coastal basin of Tanzania. Stockholm Contributions in Geology, 45 (1), 1–121.

    Myers, G.S. (1924) A new poeciliid fish from the Congo, with remarks on funduline genera. American Museum Novitates, 116, 1–9.

    Nagy, B. (2014) Nothobranchius milvertzi, a new species of killifish from the Lushiba Marsh in the Lake Mweru drainage, Zambia (Teleostei: Cyprinodontiformes: Nothobranchiidae). Ichthyological Exploration of Freshwaters, 24 (4), 347–360.

    Nagy, B. (2015) Life history and reproduction of Nothobranchius fishes. Journal of the American Killifish Association, 47 (4–6), 182–192.

    https://doi.org/10.5281/zenodo.4393325

    Nagy, B. (2018) Nothobranchius ditte, a new species of annual killifish from the Lake Mweru basin in Democratic Republic of Congo (Teleostei: Nothobranchiidae). Ichthyological Exploration of Freshwaters, 28 (2), 115–134.

    Nagy, B. & Watters, B.R. (2019) Nothobranchius eggersi. The IUCN Red List of Threatened Species, 2019, e.T60328A47183480.
    https://doi.org/10.2305/IUCN.UK.2019-3.RLTS.T60328A47183480.en

    Nagy, B. & Watters, B.R. (2020) Threatened—the world is a dangerous place for seasonal fishes. Journal of the American Killifish Association, 53 (4), 141–156.

    Nagy, B., Watters, B.R., van der Merwe, P.D.W., Cotterill, F.P.D. & Bellstedt, D.U. (2020) Review of the Nothobranchius ugandensis species group from the inland plateau of eastern Africa with descriptions of six new species (Teleostei: Nothobranchiidae). Ichthyological Exploration of Freshwaters, 30 (1), 21–73.

    http://doi.org/10.23788/IEF-1129

    Nguyen, L.T., Schmidt, H.A., von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution, 32 (1), 268–274.

    https://doi.org/10.1093/molbev/msu300

    Peters, N. (1963) Zur Embryonalentwicklung bodenlaichender Zahnkarpfen. Die Aquarien und Terrarien Zeitschrift, 16, 201–204.

    Peters, W.C.H. (1868) Über eine neue Untergattung der Flederthiere so wie über neue Gattungen und Arten von Fischen. Monatsberichte der Königlich Preussischen Akademie der Wissenschaften zu Berlin, 1868, 145–148.

    Quinn, G.P. & Keough, M.J. (2002) Experimental design and data analysis for biologists. University Press, Cambridge, 553 pp.
    https://doi.org/10.1017/CBO9780511806384

    Rambaut, A., Suchard, M. & Drummond, A. (2014) MCMC trace analysis tool: Tracer. Version 1.6. 0. Available from: http://tree.bio.ed.ac.uk/software/tracer/ (accessed 8 March 2021)

    Reichard, M. (2015) The evolutionary ecology of African annual fishes. In: Berois, N., García, G. & De Sá, R. (Eds.), Annual fishes: life history strategy, diversity, and evolution. CRC Press, Boca Raton, Florida, pp. 133–158.

    Rice, W.R. (1989) Analyzing tables of statistical tests. Evolution, 43 (1), 223–225.

    https://doi.org/10.1111/j.1558-5646.1989.tb04220.x

    Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York, New York, 1659 pp.

    Seegers, L. (1981) Ein neuer Prachtgrundkärpfling aus Tanzania - Nothobranchius spec. “Ruhoi”. Die Aquarien und Terrarien Zeitschrift, 34 (12), 409–413.

    Seegers, L. (1982) Nothobranchius eggersi n. sp. aus dem Rufiji-Einzug in Tanzania mit Bemerkungen zum Vorkommen von Nothobranchius melanospilus (Pfeffer, 1896) (Pisces, Atheriniformes, Rivulinae). Ichthyologische Ergebnisse aus Tanzania, 1. Revue de Zoologie Africaine, 96 (3), 539–548.

    Seegers, L. (1985) Prachtgrundkärpflinge, die Gattung Nothobranchius: Systematik, Vorkommen, Pflege und Zucht. Deutsche Killifisch Gemeinschaft Journal, Supplement 1, 1–48.

    Seegers, L. (1997) Killifishes of the world. Old world killis II. Aqualog, A.C.S., Mörfelden-Walldorf, 112 pp.

    Sokal, R.R. & Rohlf, F.J. (1995) Biometry: the principles and practice of statistics in biological research. 3rd Edition. Freeman, New York, 880 pp.

    Sokal, R.R. & Rohlf, F.J. (2009) Introduction to biostatistics. 2nd Edition. Dover Publications, Mineola, 382 pp.

    Thieme, M.L., Abell, R., Stiassny, M.L.J., Skelton, P.H., Lehner, B., Teugels, G.G., Dinerstein, E., Kamdem-Toham, A., Burgess, N.D. & Olson, D. (2005) Freshwater ecoregions of Africa and Madagascar: a conservation assessment. Island Press, Washington, Covelo & London, 483 pp.

    Vanderplank, F.L. (1940) A study of Nothobranchius taeniopygus in its natural habitat. The Aquarist, 10, 247–249.

    van der Merwe, P.D.W., Cotterill, F.P.D., Kandziora, M., Watters, B.R., Nagy, B., Genade, T., Flügel, T. J., Svendsen, D.S. & Bellstedt, D.U. (2020) Genomic Fingerprints of Palaeogeographic History: The tempo and mode of Rift tectonics across tropical Africa has shaped the diversification of the killifish genus Nothobranchius (Teleostei: Cyprinodontiformes). Molecular Phylogenetics and Evolution, 158, 106988.

    https://doi.org/10.1016/j.ympev.2020.106988.

    Vinciguerra, D. (1897) Pesci raccolti dal Cap. V. Bottego, durante la sua seconda spedizione nelle regione dei Somali e dei Galla. Annali del Museo Civico di Storia Naturale Giacomo Doria, Genova, Series 2, 17 (37), 343–364.

    Watters, B.R. (2009) The ecology and distribution of Nothobranchius fishes. Journal of the American Killifish Association, 42 (2), 37–76.

    Watters, B.R. (2014) A classification of Nothobranchius fish habitats. Journal of the American Killifish Association, 47 (4–6), 152–180.

    Watters, B.R., Wildekamp, R.H. & Cooper, B.J. (1998) Zwei neue Nothobranchius-Arten aus der Küstenebene Tansanias. Deutsche Killifisch Gemeinschaft Journal, 30 (3), 52–63.

    Watters, B.R., Cooper, B.J. & Wildekamp, R.H. (2007) Description of Nothobranchius cardinalis spec. nov. (Cyprinodontiformes: Aplocheilidae), an annual fish from the Mbwemkuru River basin, Tanzania. Journal of the American Killifish Association, 40 (5–6), 129–145.

    Watters, B.R., Nagy, B., van der Merwe, P.D.W., Cotterill, F.P.D. & Bellstedt, D.U. (2019) Review of the Nothobranchius taeniopygus species group from central and western Tanzania with descriptions of five new species and redescription of Nothobranchius taeniopygus (Teleostei: Nothobranchiidae). Ichthyological Exploration of Freshwaters, 29 (3), 239–278.
    http://doi.org/10.23788/IEF-1110

    Watters, B.R., Nagy, B., van der Merwe, P.D.W., Cotterill, F.P.D. & Bellstedt, D.U. (2020) Redescription of the seasonal killifish species Nothobranchius ocellatus and description of a related new species Nothobranchius matanduensis, from eastern Tanzania (Teleostei: Nothobranchiidae). Ichthyological Exploration of Freshwaters, 30 (2), 151–178.
    https://doi.org/10.23788/IEF-1149

    Wildekamp, R.H. (1977) Nothobranchius lourensi sp. nov. und Nothobranchius janpapi sp. nov., zwei neue Rivulinen aus Ost Afrika. Das Aquarium, 11 (98), 326–331.

    Wildekamp, R.H. (2004) A world of killies. Atlas of the oviparous cyprinodontiform fishes of the world. Vol. IV. American Killifish Association, Elyria, 398 pp.

    Wildekamp, R.H. & Haas, R. (1992) Redescription of Nothobranchius microlepis, description of two new species from northern Kenya and southern Somalia, and note on the status of Paranothobranchius (Cyprinodontiformes: Aplocheilidae). Ichthyological Exploration of Freshwaters, 3 (1), 1–16.

    Wildekamp, R.H., Shidlovskiy, K.M. & Watters, B.R. (2009) Systematics of the Nothobranchius melanospilus species group (Cyprinodontiformes: Nothobranchiidae) with description of two new species from Tanzania and Mozambique. Ichthyological Exploration of Freshwaters, 20 (3), 237–254.

    Wiley, E.O. (1978) The evolutionary species concept reconsidered. Systematic Zoology, 27 (1), 17–26.

    https://doi.org/10.2307/2412809

    Yang, Z. (2007) PAML 4: phylogenetic analysis by maximum likelihood. Molecular Biology and Evolution, 24 (8), 1586–1591.

    https://doi.org/10.1093/molbev/msm088

    Yule, G.U. (1925) A mathematical theory of evolution, based on the conclusions of Dr. J. C. Willis, F.R.S. Philosophical Transactions of the Royal Society of London, Series B, 213, 21–87.

    https://doi.org/10.1098/rstb.1925.0002

    Zar, J.H. (2010) Biostatistical analysis. Fifth edition. Pearson/Prentice-Hall, Upper Saddle River, xii + 944 pp.

    Zuur, A.F., Ieno, E.N. & Smith, G.M. (2007) Analysing ecological data (statistics for biology and health). Springer, New York, xxvi + 672 pp.

    https://doi.org/10.1007/978-0-387-45972-1