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Type: Article
Published: 2021-03-25
Page range: 261–288
Abstract views: 165
PDF downloaded: 11

Molecular phylogenetics, PCA, and MFA recover a new species of Cyrtodactylus (Squamata: Gekkonidae) from an isolated sandstone massif in northwestern Cambodia

Herpetology Laboratory, Department of Biology, La Sierra University, 4500 Riverwalk Parkway, Riverside, California 92515, USA.
Museum Natur und Mensch, Gerberau 32, 79098 Freiburg, GERMANY.
Wild Earth Allies, 77a, Street Beton, Bayap Village, Sangkat Phnom Penh Thmei, Khan Sen Sok, Phnom Penh, CAMBODIA
Zoologisches Forschungsmusem Alexander Koenig, Adenauerallee 160, D-5311 Bonn, GERMANY
Department of Research and Conservation, Allwetterzoo, Sentruper Str. 315, 48161 Münster, GERMANY
Department of Vertebrate Zoology, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow 119234, RUSSIA. Joint Russian-Vietnamese Tropical Research and Technological Center, Nghia Do, Cau Giay, Hanoi, VIETNAM
Reptilia gecko habitat island phylogeny Indochina systematics Siem Reap Province Phnom Kulen National Park

Abstract

The integrated results of maximum likelihood (ML) and Bayesian inference (BI) analyses, principal component analyses (PCA), and a multiple factor analysis (MFA) recover a new, widely allopatric species of the Cyrtodactylus intermedius species group. Cyrtodactylus kulenensis sp. nov is endemic to the Phnom Kulen sandstone massif of the Phnom Kulen National Park, Siem Reap Province, in the lowlands of northwestern Cambodia. A phylogenetic analysis from a short read (275 base pairs) of the mitochondrial gene NADH dehydrogenase subunit 2 (ND2) from C. kulenensis sp. nov. was aligned with 1449 base pairs from all other species in the intermedius group.  The analysis recovered C. kulenensis sp. nov. as the sister species to a lineage composed of populations from the widely separated hilly regions of Sa Keao and Sakaerat in eastern Thailand. Multivariate (PCA, DAPC, and MFA) and univariate analyses (ANOVA) using combinations of meristic (scale counts), mensural (morphometric), and categorical (color pattern and morphology) characters from 52 specimens encompassing all species of the intermedius group clearly demonstrate C. kulenensis sp. nov. is significantly different and discretely diagnosable from all other species in the intermedius group. This new discovery further highlights the herpetological diversity and high levels of range-restricted endemism in basin-habitat-island landscapes throughout Indochina and the continued need for field work in the landscapes that remain unsurveyed.

 

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