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Type: Article
Published: 2021-03-12
Page range: 151–172
Abstract views: 190
PDF downloaded: 14

Three new species of Ametrodiplosis (Diptera: Cecidomyiidae) from Japan, with a key to the Japanese species and a molecular phylogenetic analysis

The Botanical Gardens, Graduate School of Science, The University of Tokyo, Tokyo ​112–0001 Japan
Entomological Laboratory, Faculty of Agriculture, Kyushu University, Fukuoka 819–0395, Japan.
The Botanical Gardens, Graduate School of Science, The University of Tokyo, Tokyo ​112–0001 Japan
Laboratory of Systems Ecology, Faculty of Agriculture, Saga University, Saga 840–8502, Japan.
The Botanical Gardens, Graduate School of Science, The University of Tokyo, Tokyo ​112–0001 Japan
Diptera Gall midge Clinodiplosini Apocynaceae Caryophyllaceae DNA barcode


Ametrodiplosis Rübsaamen (Diptera: Cecidomyiidae: Clinodiplosini) is a mostly Holarctic gall midge genus whose species are associated with a wide range of seed plant families, either as gall-inducers or inquilines. In this study, we describe three species of Ametrodiplosis from Japan: A. adetos n. sp. feeding in the flowers of Tylophora aristolochioides Miq. (Apocynaceae); A. aeroradicis n. sp. inducing aerial root galls on Trachelospermum asiaticum (Sieb. et Zucc.) Nakai and T. gracilipes var. liukiuense (Apocynaceae); and A. stellariae n. sp. forming leaf bud galls on Stellaria uliginosa Murray var. undulata (Thunb.) Ohwi (Caryophyllaceae). A molecular phylogenetic analysis using mitochondrial COI and ribosomal 16S genes and nuclear ribosomal 28S gene were conducted for the three new Ametrodiplisis species and other clinodiplosine taxa sequences available in GenBank. The analysis supported the monophyly of Ametrodiplosis despite the variable life history of the three species. In addition, it indicated very low intraspecific genetic divergence among the individuals from different localities and/or host plants. A taxonomic key to the three new Japanese species of Ametrodiplosis is provided.



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