Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2021-01-27
Page range: 131–139
Abstract views: 237
PDF downloaded: 121

First record of the West Nile virus bridge vector Culex modestus Ficalbi (Diptera: Culicidae) in Belgium, validated by DNA barcoding

The Unit of Entomology, Dept. Biomedical Sciences, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium.
Royal Belgian Institute of Natural Sciences (BopCo & Scientific Heritage Service), Vautierstraat 29, 1000 Brussels, Belgium.
The Unit of Entomology, Dept. Biomedical Sciences, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium.
Royal Museum for Central Africa (BopCo & Biology Department), Leuvensesteenweg 17, 3080 Tervuren, Belgium.
Royal Belgian Institute of Natural Sciences (BopCo & Scientific Heritage Service), Vautierstraat 29, 1000 Brussels, Belgium.
Royal Belgian Institute of Natural Sciences (BopCo & Scientific Heritage Service), Vautierstraat 29, 1000 Brussels, Belgium.
Royal Museum for Central Africa (BopCo & Biology Department), Leuvensesteenweg 17, 3080 Tervuren, Belgium.
Royal Belgian Institute of Natural Sciences (BopCo & Scientific Heritage Service), Vautierstraat 29, 1000 Brussels, Belgium.
Royal Museum for Central Africa (BopCo & Biology Department), Leuvensesteenweg 17, 3080 Tervuren, Belgium.
Royal Belgian Institute of Natural Sciences (BopCo & Scientific Heritage Service), Vautierstraat 29, 1000 Brussels, Belgium. Evolutionary Ecology Group, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium.
The Unit of Entomology, Dept. Biomedical Sciences, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium.
The Unit of Entomology, Dept. Biomedical Sciences, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium. Outbreak Research team, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium.
Diptera biodiversity DNA-based identification larva monitoring mosquito new record

Abstract

A thorough knowledge of the presence and spatio-temporal distribution patterns of vector species are pivotal to assess the risk of mosquito-borne diseases in Europe. In 2018, a Culex larva was collected during routine monitoring activities to intercept exotic Aedes mosquito species in the port of Antwerp (Kallo, Belgium). The larva, collected from a pond in mid-September, was morphologically identified as Culex modestus, and this identification was subsequently confirmed by COI barcoding. It is the first confirmed record of this West Nile virus bridge vector in Belgium. The present study also demonstrates the value of DNA-based identification techniques to validate the presence of potential vector species.

 

References

  1. Balenghien, T., Fouque, F., Sabatier, P. & Bicout, D.J. (2006) Horse-, bird-, and human-seeking behavior and seasonal abundance of mosquitoes in a West Nile virus focus of southern France. Journal of Medical Entomology, 43 (5), 936–946.

    https://doi.org/10.1093/jmedent/43.5.936

    Balenghien, T., Vazeille, M., Reiter, P., Schaffner, F., Zeller, H. & Bicout, D.J. (2007) Evidence of laboratory vector competence of Culex modestus for West Nile virus. Journal of the American Mosquito Control Association, 23 (2), 233–236.

    https://doi.org/10.2987/8756-971X(2007)23[233:EOLVCO]2.0.CO;2

    Becker, N. (2008) Influence of climate change on mosquito development and mosquito-borne diseases in Europe. Parasitology Research, 103 (1), 19–28.

    https://doi.org/10.1007/s00436-008-1210-2

    Becker, N., Petric, D., Zgomba, M., Boase, C., Madon, M., Dahl, C. & Kaiser, A. (2010) Mosquitoes and their control. 2nd Edition. Springer-Verlag, Berlin and Heidelberg, xxx + 577 pp.

    Boukraa, S., Dekoninck, W., Versteirt, V., Schaffner, F., Coosemans, M., Haubruge, E. & Francis, F. (2015) Updated checklist of the mosquitoes (Diptera: Culicidae) of Belgium. Journal of Vector Ecology, 40 (2), 398–407.

    https://doi.org/10.1111/jvec.12180

    Boukraa, S., Zimmer, J.-Y., Simonon, G., Haubruge, E. & Francis, F. (2011) Stand structure of mosquitoes (Diptera Culicidae) in farms and establishment of a new potential vector of West Nile Virus for Belgium. 63th International Symposium on Crop Protection, Gent, Belgium, 2011, 287.

    Brugman, V.A., England, M.E., Stoner, J., Tugwell, L., Harrup, L.E., Wilson, A.J., Medlock, J.M., Logan, J.G., Fooks, A.R., Mertens, P.P.C., Johnson, N. & Carpenter, S. (2017) How often do mosquitoes bite humans in southern England? A standardised summer trial at four sites reveals spatial, temporal and site-related variation in biting rates. Parasites & Vectors, 10 (1), 420–420.

    https://doi.org/10.1186/s13071-017-2360-9

    Brugman, V.A., Hernandez-Triana, L.M., Medlock, J.M., Fooks, A.R., Carpenter, S. & Johnson, N. (2018) The role of Culex pipiens L. (Diptera: Culicidae) in virus transmission in Europe. International Journal of Environmental Research and Public Health, 15 (2), 389.

    https://doi.org/10.3390/ijerph15020389

    Calzolari, M. (2016) Mosquito-borne diseases in Europe: an emerging public health threat. Reports in Parasitology, 5, 1–12.

    https://doi.org/10.2147/RIP.S56780

    Deblauwe, I., De Wolf, K., Smitz, N., Vanslembrouck, A., Schneider A., De Witte, J., Verlé, I., Dekoninck, W., De Meyer, M., Backeljau, T., Gombeer, S., Meganck, K., Van Bourgonie, Y.-R., Vanderheyden, A., Müller, R. & Van Bortel, W. (2020) Monitoring of exotic mosquitoes in Belgium (MEMO): Final Report Phase 7 Part 1: MEMO results. NEHAP, Antwerp, Belgium, 100 pp.

    ECDC (2018) Local transmission of dengue fever in France and Spain – 2018. European Centre for Disease Prevention and Control, Stockholm, Sweden, 13 pp.

    ECDC (2020) Mosquito maps, European Centre for Disease Prevention and Control and European Food Safety Authority, Stockholm. Available from: https://ecdc.europa.eu/en/disease-vectors/surveillance-and-disease-data/mosquito-maps (accessed 26 August 2020)

    Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3 (5), 294–299.

    Golding, N., Nunn, M.A., Medlock, J.M., Purse, B.V., Vaux, A.G.C. & Schäfer, S.M. (2012) West Nile virus vector Culex modestus established in southern England. Parasites & Vectors, 5 (1), 32.

    https://doi.org/10.1186/1756-3305-5-32

    Gunay, F., Picard, M. & Robert, V. (2018) MosKeyTool, an interactive identification key for mosquitoes of Euro-Mediterranean. Version 2.1. 2018. Available from: https://www.medilabsecure.com/moskeytool.html (accessed 26 August 2020)

    Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41 (41), 95–98.

    Harbach, R.E. (2012) Culex pipiens: Species versus species complex – taxonomic history and perspective. Journal of the American Mosquito Control Association, 28 (4s), 10–23.

    https://doi.org/10.2987/8756-971X-28.4.10

    Hebert, P.D.N., Cywinska, A., Ball, S.L. & Dewaard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London, Series B, Biological Sciences, 270 (1512), 313–321.

    https://doi.org/10.1098/rspb.2002.2218

    Hernandez-Triana, L. M., Brugman, V. A., Pramual, P., Barrero, E., Nikolova, N. I., Ruiz-Arrondo, I., Kaiser, A., Kruger, A., Lumley, S., Osorio, H. C., Ignjatovic-Cupina, A., Petric, D., Laure Setier-Rio, M., Bodker, R. & Johnson, N. (2020) Genetic diversity and population structure of Culex modestus across Europe: does recent appearance in the United Kingdom reveal a tendency for geographical spread? Medical and Veterinary Entomology, 34 (1), 86–96.

    https://doi.org/10.1111/mve.12412

    Hubálek, Z. (2008) Mosquito-borne viruses in Europe. Parasitology Research, 103 (1), 29–43.

    https://doi.org/10.1007/s00436-008-1064-7

    Hubálek, Z. & Halouzka, J. (1999) West Nile fever--a reemerging mosquito-borne viral disease in Europe. Emerging Infectious Diseases, 5 (5), 643–650.

    https://doi.org/10.3201/eid0505.990505

    Ibáñez-Justicia, A., Smitz, N., den Hartog, W., van de Vossenberg, B., De Wolf, K., Deblauwe, I., Van Bortel, W., Jacobs, F., Vaux, A.G.C., Medlock, J.M. & Stroo, A. (2020) Detection of exotic mosquito species (Diptera: Culicidae) at international airports in Europe. International Journal of Environmental Research and Public Health, 17 (10), 3450.

    https://doi.org/10.3390/ijerph17103450

    Jacobs, I., Feys, S., Nijs, D., Gielen, G., Verbelen, D. & Lambrechts, J. (2020) s.n. In: Monitoring van ontsnipperings-maatregelen voor herpetofauna in Vlaanderen. Eindrapport. Rapport Natuurpunt Studie 9. Mechelen, Belgium, pp. 82–89. Available from: https://archief-algemeen.omgeving.vlaanderen.be/xmlui/handle/acd/262679 (accessed 26 August 2020)

    Lock, K., Dekoninck, W., Adriaens, T. & Goethals, P.L.M. (2012) Distribution and ecology of mosquito larvae (Diptera: Culicidae) in Flanders (Belgium). Belgian Journal of Zoology, 142 (2), 138–142.

    Lundström, J.O. (1999) Mosquito-borne viruses in western Europe: a review. Journal of Vector Ecology, 24 (1), 1–39.

    Medlock, J.M., Hansford, K.M., Versteirt, V., Cull, B., Kampen, H., Fontenille, D., Hendrickx, G., Zeller, H., Van Bortel, W. & Schaffner, F. (2015) An entomological review of invasive mosquitoes in Europe. Bulletin of Entomological Research, 25 (6), 1–27.

    https://doi.org/10.1017/S0007485315000103

    Medlock, J.M. & Vaux, A.G.C. (2015) Impacts of the creation, expansion and management of English wetlands on mosquito presence and abundance—developing strategies for future disease mitigation. Parasites & Vectors, 8 (1), 142.

    https://doi.org/10.1186/s13071-015-0751-3

    Papa, A., Danis, K., Baka, A., Bakas, A., Dougas, G., Lytras, T., Theocharopoulos, G., Chrysagis, D., Vassiliadou, E., Kamaria, F., Liona, A., Mellou, K., Saroglou, G. & Panagiotopoulos, T. (2010) Ongoing outbreak of West Nile virus infections in humans in Greece, July – August 2010. Eurosurveillance, 15 (34), 19644.

    https://doi.org/10.2807/ese.15.34.19644-en

    Ponçon, N., Balenghien, T., Toty, C., Baptiste Ferre, J., Thomas, C., Dervieux, A., L’Ambert, G., Schaffner, F., Bardin, O. & Fontenille, D. (2007) Effects of local anthropogenic changes on potential malaria vector Anopheles hyrcanus and West Nile virus vector Culex modestus, Camargue, France. Emerging Infectious Diseases, 13 (12), 1810–1815.

    https://doi.org/10.3201/eid1312.070730

    Radrova, J., Seblova, V. & Votypka, J. (2013) Feeding behavior and spatial distribution of Culex mosquitoes (Diptera: Culicidae) in wetland areas of the Czech Republic. Journal of Medical Entomology, 50 (5), 1097–1104.

    https://doi.org/10.1603/ME13029

    Randolph, S.E. & Rogers, D.J. (2010) The arrival, establishment and spread of exotic diseases: patterns and predictions. Nature Reviews Microbiology, 8 (5), 361–371.

    https://doi.org/10.1038/nrmicro2336

    Reusken, C., De Vries, A., Den Hartog, W., Braks, M. & Scholte, E.J. (2010) A study of the circulation of West Nile virus in mosquitoes in a potential high-risk area for arbovirus circulation in the Netherlands, “De Oostvaardersplassen”. European Mosquito Bulletin, 28, 69–83.

    Ries, C. (2019) Three mosquito species new to Luxembourg: Culex modestus, Uranotaenia unguiculata and Culiseta glaphyroptera. Musée national d’histoire naturelle, Luxembourg. Available from: https://mosquitoes.lu/three-mosquito-species-new-to-luxembourg-culex-modestus-uranotaenia-unguiculata-and-culiseta-glaphyroptera/ (accessed 26 August 2020)

    Rochlin, I., Faraji, A., Ninivaggi, D.V., Barker, C.M. & Kilpatrick, A.M. (2016) Anthropogenic impacts on mosquito populations in North America over the past century. Nature Communications, 7 (1), 13604.

    https://doi.org/10.1038/ncomms13604

    Roiz, D., Ruiz, S., Soriguer, R. & Figuerola, J. (2015) Landscape effects on the presence, abundance and diversity of mosquitoes in Mediterranean wetlands. PLoS One, 10 (6), e0128112.

    https://doi.org/10.1371/journal.pone.0128112

    Roiz, D., Wilson, A.L., Scott, T.W., Fonseca, D.M., Jourdain, F., Muller, P., Velayudhan, R. & Corbel, V. (2018) Integrated Aedes management for the control of Aedes-borne diseases. PLOS Neglected Tropical Diseases, 12 (12), e0006845.

    https://doi.org/10.1371/journal.pntd.0006845

    Sohier, C. & Grootaert, P. (2015) Muggenproblematiek van het Linkerscheldeoevergebied. Royal Belgian Institute of Natural Sciences, Brussel, pp. 13–14.

    Thompson, R., Martin Del Campo, J. & Constenla, D. (2020) A review of the economic evidence of Aedes-borne arboviruses and Aedes-borne arboviral disease prevention and control strategies. Expert Review of Vaccines, 19 (2), 143–162.

    https://doi.org/10.1080/14760584.2020.1733419

    Versteirt, V., Boyer, S., Damiens, D., De Clercq, E.M., Dekoninck, W., Ducheyne, E., Grootaert, P., Garros, C., Hance, T., Hendrickx, G., Coosemans, M. & Van Bortel, W. (2013) Nationwide inventory of mosquito biodiversity (Diptera: Culicidae) in Belgium, Europe. Bulletin of Entomological Research, 103 (2), 193–203.

    https://doi.org/10.1017/S0007485312000521

    Versteirt, V., Nagy, Z.T., Roelants, P., Denis, L., Breman, F.C., Damiens, D., Dekoninck, W., Backeljau, T., Coosemans, M. & Van Bortel, W. (2015) Identification of Belgian mosquito species (Diptera: Culicidae) by DNA barcoding. Molecular Ecology Resources, 15 (2), 449–457.

    https://doi.org/10.1111/1755-0998.12318

    Vlaskamp, D.R., Thijsen, S.F., Reimerink, J., Hilkens, P., Bouvy, W.H., Bantjes, S.E., Vlaminckx, B.J., Zaaijer, H., van den Kerkhof, H.H., Raven, S.F. & Reusken, C.B. (2020) First autochthonous human West Nile virus infections in the Netherlands, July to August 2020. Eurosurveillance, 25 (46), 2001904.

    https://doi.org/10.2807/1560-7917.ES.2020.25.46.2001904

    Zeller, H., Lenglet, A. & Van Bortel, W. (2010) West Nile virus: the need to strengthen preparedness in Europe. Eurosurveillance, 15 (34), 19647.

    https://doi.org/10.2807/ese.15.34.19647-en

    Zeller, H.G. & Schuffenecker, I. (2004) West Nile virus: An overview of its spread in Europe and the Mediterranean Basin in contrast to its spread in the Americas. European Journal of Clinical Microbiology and Infectious Diseases, 23 (3), 147–156.

    https://doi.org/10.1007/s10096-003-1085-1

    Ziegler, U., Lühken, R., Keller, M., Cadar, D., van der Grinten, E., Michel, F., Albrecht, K., Eiden, M., Rinder, M., Lachmann, L., Höper, D., Vina-Rodriguez, A., Gaede, W., Pohl, A., Schmidt-Chanasit, J. & Groschup, M.H. (2019) West Nile virus epizootic in Germany, 2018. Antiviral Research, 162, 39–43.

    https://doi.org/10.1016/j.antiviral.2018.12.005