Skip to main content Skip to main navigation menu Skip to site footer
Article
Published: 2021-01-14

A new frog species of the genus Odorrana (Anura: Ranidae) from Yunnan, China

Key Laboratory for Conserving Wildlife with Small Populations in Yunnan, Southwest Forestry University, Kunming 650224, Yunnan, China
Key Laboratory for Conserving Wildlife with Small Populations in Yunnan, Southwest Forestry University, Kunming 650224, Yunnan, China
Zhejiang Forest Resource Monitoring Center, Hangzhou 310020
Wildlife Health Ghent, Department of Pathology, Bacteriology and Avian Diseases, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
Gongshan Administrative suboffice of Gaoligongshan National Nature Reserve, Gongshan 673500, Yunnan, China
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
Key Laboratory for Conserving Wildlife with Small Populations in Yunnan, Southwest Forestry University, Kunming 650224, Yunnan, China
Amphibia China-Myanmar border Odorrana dulongensis sp. nov. taxonomy Gaoligong Dulongjiang

Abstract

The Chinese-Myanmar border area forms part of a long-acknowledged biodiversity hotspot. This region is characterised by dramatic topography and diverse landscapes, which support a high degree of biodiversity and endemism that remains largely understudied. Based on recent survey efforts we here describe a new frog species of the genus Odorrana from this area. Found in Dulongjiang village, Yunnan, China, the new species differs from a morphological perspective from its congeners through the combination of a grass-green dorsum with black posterior spots, absence of dorsolateral folds, a distinct pineal body, presence of supratympanic folds, absence of outer metacarpal, metatarsal and supernumerary tubercles, and a pair of external subgular vocal sacs. From a molecular perspective, pairwise genetic distances of 4.8% to 11.6% for DNA sequences of the mitochondrial gene for 16S ribosomal RNA differentiate the new species from other Odorrana. Our mitochondrial phylogeny suggests that the new species is a deeply divergent genetic lineage clustering with the clade including Odorrana wuchuanensis, and the Odorrana andersonii and Odorrana margaretae groups. The discovery of this new species emphasizes the need for further herpetological studies in the China-Myanmar border region.

 

References

  1. AmphibiaWeb (2020) Information on amphibian biology and conservation. Amphibia Web, Berkeley, California. Available from: http://amphibiaweb.org/ (accessed 15 February 2020)

    Ao, J.M., Bordoloi, S. & Ohler, A. (2003) Amphibian fauna of Nagaland with nineteen new records from the state including five records for India. Zoos’ Print Journal, 18 (6), 1117–1125.

    https://doi.org/10.11609/JoTT.ZPJ.18.6.1117-25

    Bain, R.H., Lathrop, A., Murphy, R.W., Orlov, N.L. & Cuc, H.T. (2003) Cryptic species of a cascade frog from Southeast Asia: Taxonomic revisions and descriptions of six new species. American Museum Novitates, 3417, 1–60.

    https://doi.org/10.1206/0003-0082(2003)417%3C0001:CSOACF%3E2.0.CO;2

    Bain, R.H. & Stuart, B.L. (2005) A new species of cascade frog (Amphibia: Ranidae) from Thailand, with new data on Rana banaorum and Rana morafkai. Natural History Bulletin of the Siam Society, 53 (1), 3–16.

    Bain, R.H., Stuart, B.L., Nguyen, T.Q., Che, J. & Rao, D.Q. (2009) A new Odorrana (Amphibia: Ranidae) from Vietnam and China. Copeia, 2009 (2), 348–362.

    https://doi.org/10.1643/CH-07-195

    Chen, L.Q., Murphy, R.W., Lathrop, A., Ngo, A., Orlov, N.L., Ho, C.T., Somorjai, I.L.M. (2005) Taxonomic chaos in Asian ranid frogs: An initial phylogenetic resolution. Herpetological, J15 (4), 231–243.

    Chen, X.H., Chen, Z., Jiang, J.P., Qiao, L., Lu, Y.Q., Zhou, K.Y., Zheng, G.M., Zhai, X.F. & Liu, J.X. (2013) Molecular phylogeny and diversification of the genus Odorrana (Amphibia, Anura, Ranidae) inferred from two mitochondrial genes. Molecular Phylogenetics and Evolution, 69 (3), 1196–1202.

    https://doi.org/10.1016/j.ympev.2013.07.023

    Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) JModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9, 772.

    https://doi.org/10.1038/nmeth.2109

    Fei, L., Hu, S.Q., Ye, C.Y. & Huang, Y.Z. (2009) Fauna Sinica. In: Amphibia Vol. 2 Anura. Science Press, Beijin, 957 pp.

    Fei, L., Ye, C.Y. & Jiang, J.P. (2012) Colored atlas of Chinese Amphibians and their distributions. Sichuan Publishing House of Science and Technology, Chengdu, 619 pp.

    Fei, L., Ye, C.Y. & Li, C. (2001) Descriptions of two new species of the genus Odorrana in China (Anura: Ranidae). Zoological Systematics, 26 (1), 108–114.

    Feng, Y, Blackburn, D.C., Liang, D, Hillis, D.M., Wake, D.B., Cannatella, D.C. & Zhang, P. (2017) Phylogenomics reveals rapid, simultaneous diversification of three major clades of Gondwanan frogs at the Cretaceous-Paleogene boundary. Proceedings of the National Academy of Sciences of the United States of America, 114, E5864–E5870.

    https://doi.org/10.1073/pnas.1704632114

    Frost, D.R., Grant, T., Faivovich, J., Bain, R., Haas, A., Haddad, C.F.B., Sa, R.O., Channing, A., Wilkinson, M., Donnellan, S.C., Raxworthy, C., Campbell, J.A., Blotto, B.L., Moler, P., Drewes, R.C., Nussbaum, R.A., Lynch, J.D., Green, D.M. & Wheeler, W.C. (2006) The amphibian tree of life. Bulletin of the American Museum of Natural History, 279, 1–291.

    https://doi.org/10.1206/0003-0090(2006)297[0001:TATOL]2.0.CO;2

    Holt, B.G., Lessard, J.P., Borregaard, M.K., Fritz, S.A., Araujo, M.B., Dimitrov, D., Fabre, P., Graham, C.H., Graves, G.R., Jonsson, K.A., Noguesbravo, D., Wang, Z. H., Whittaker, R. J., Fjeldsa, J. & Rahbek, C. (2013) An up-date of Wallace’s zoogeographic regions of the world. Science, 339, 4–78.

    https://doi.org/10.1126/science.1228282

    Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution, 30, 772–780.

    https://doi.org/10.1093/molbev/mst010

    Kurabayashi, A., Yoshikawa, N., Sato, N., Hayashi, Y., Oumi, S., Fujii, T., Sumida, M. (2010) Complete mitochondrial DNA sequence of the endangered frog Odorrana ishikawae (family Ranidae) and unexpected diversity of mt gene arrangements in ranids. Molecular Phylogenetics and Evolution, 56 (2), 543–553.

    https://doi.org/10.1016/j.ympev.2010.01.022

    Li, P.P., Lu, Y.Y. & Rao, D.Q. (2008) A new species of cascade frog (Amphibia, Ranidae) from Tibet, China. Zoological Systematics, 33 (3), 537–541.

    Li, S., Xu, N., Lv, J., Jiang, J., Wei, G. &Wang, B. (2018) A new species of the odorous frog genus Odorrana (Amphibia, Anura, Ranidae) from southwestern China. PeerJ, 6, e569.

    https://doi.org/10.7717/peerj.5695

    Marchese, C. (2015) Biodiversity hotspots: A shortcut for a more complicated concept. Global Ecology and Conservation, 3, 297–309.

    https://doi.org/10.1016/j.gecco.2014.12.008

    Masafumi, M. (1994) A taxonomic study of the Rana narina complex, with description of three new species (Amphibia: Ranidae). Zoological Journal of the Linnean Society, 111 (4), 385–415.

    https://doi.org/10.1111/j.1096-3642.1994.tb01489.x

    Matsui, M. & Jaafar, I. (2006) A new cascade frog of the subgenus Odorrana from Peninsular Malaysia. Zoological Science, 23 (7), 647–651.

    https://doi.org/10.2108/zsj.23.647

    Matsui, M., Shimada, T., Ota, H. &Tanaka-Ueno, T. (2005) Multiple invasions of the Ryukyu Archipelago by Oriental frogs of the subgenus Odorrana with phylogenetic reassessment of the related subgenera of the genus Rana. Molecular Phylogenetics and Evolution, 37 (3), 733–742.

    https://doi.org/10.1016/j.ympev.2005.04.030

    Mo, Y.M., Chen, W.C, Wu, H.Y., Zhang, W. & Zhou, S.C. (2015) A new species of Odorrana inhabiting complete darkness in a karst cave in Guangxi, China. Asian Herpetological Research, 6 (1), 11–17

    Myers, N., Mittermeier, R.A., Mittermeier, C.G., Fonseca, G.A. & Kent, J. (2000) Biodiversity hotspots for conservation priorities. Nature, 403 (6772), 853–858.

    https://doi.org/10.1038/35002501

    Orlov, N.L., Natalia, B.A. & Cuc, H.T. (2006) A new cascade frog (Amphibia: Ranidae) from central Vietnam. Russian Journal of Herpetology, 13 (2), 155–163.

    Orlov, N.L., Ngat, L.N. & Cuc, H.T. (2003) A new species of cascade frog from north Vietnam (Ranidae, Anura). Russian Journal of Herpetology, 10 (2), 123–134.

    Pham, C.T., Nguyen, T.Q., Le, M.D., Bonkowski, M. & Ziegler, T. (2016) A new species of Odorrana (Amphibia: Anura: Ranidae) from Vietnam. Zootaxa, 4084 (3), 421–435.

    https://doi.org/10.11646/zootaxa.4084.3.7

    Ronquist, F., Teslenko, M., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large mod space. Systematic Biology, 61, 539–542.

    https://doi.org/10.1093/sysbio/sys029

    Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A laboratory Manual, Sec Ed. Cold Spring Harbor Laboratory Press, Cold Springs Harbor, New York,1659 pp.

    Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, weighting and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America, 87 (6), 651–701.

    https://doi.org/10.1093/aesa/87.6.651

    Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30, 131–1313.

    https://doi.org/10.1093/bioinformatics/btu033

    Stuart, B.L. (2008) The phylogenetic problem of Huia (Amphibia: Ranidae). Molecular Phylogenetics and Evolution, 46 (1), 49–60.

    https://doi.org/10.1016/j.ympev.2007.09.016

    Stuart, B.L. & Bain, R.H. (2005) Three new species of spinule-bearing frogs allied to Rana megatympanum Bain, Lathrop, Murphy, Orlov, and Ho, 2003, from Laos and Vietnam. Herpetologica, 61 (4), 478–492.

    https://doi.org/10.1655/05-06.1

    Stuart, B.L. & Chanard, T. (2005) Two new Huia (Amphibia: Ranidae) from Laos and Thailand. Copeia, 2005 (2), 279–289.

    https://doi.org/10.1643/CH-04-137R3

    Stuart, B.L., Chuaynkern, Y., Chanard, T. & Inger, R.F. (2006) Three species of frogs and a new tadpole from eastern Thailand. Fieldiana: Zoology, New Series, 111, 1–19.

    https://doi.org/10.3158/0015-0754(2006)187[1:TNSOFA]2.0.CO;2

    Su, X., Wu, X.B., Yan, P., Cao, S.Y. & Hu, Y.L. (2007) Rearrangement of a mitochondrial tRNA gene of the concave-eared torrent frog, Amolops tormotus. Gene, 394, 25–34.

    https://doi.org/10.1016/j.gene.2007.01.022

    Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.

    https://doi.org/10.1093/molbev/mst197

    Tran, T.T., Orlov, N.L. & Nguyen, T.T. (2011) A new species of cascade frog of Odorrana Fei, Yi et Huang, 1990 genus (Amphibia: Anura: Ranidae) from Bac Giang province (Yen Tu Mountain Range, northeast Vietnam). Russian Journal of Herpetology, 15 (3), 212–224.

    Wang, Y., Lau, M.W., Yang, J., Chen, G., Liu, Z., Pang, H. & Liu, Y. (2015) A new species of the genus Odorrana (Amphibia: Ranidae) and the first record of Odorrana bacboensis from China. Zootaxa, 3999 (2), 235–254.

    https://doi.org/10.11646/zootaxa.3999.2.4

    Wu, L., Xu, R., Dong, Q., Li, D.J. & Liu J.S. (1983) A new species of Rana and records of amphibians from Guizhou Province. Acta Zoologica Sinica, 29, 66–70.

    Xue, J.R. (1995) Goligongshan National Nature Reserve. China Forestry Press, Beijing, 677 pp.

    Yang, D.T. (2008) Amphibia and Reptilia of Yunnan. Yunnan Publishing Group Corporation, Yunnan Science and Technology Press, Kunming, 411 pp.

    Yang, D.T., Li, S.M. (1980) A new species of the genus Rana from Yunnan. Zoological Research, 1 (2), 261–264.

    Yuan, Z.Y., Liu, X.L., Wang, K., Wang, J., Chen, J.M., Jin, J.Q., Wei, P.F. & Zhou, J.J., Che, J. (2019a) Nidirana chapaensis (Bourret, 1937), one additional anuran species for the amphibian fauna of China. Zootaxa, 4571 (4), 580–588.

    https://doi.org/10.11646/zootaxa.4571.4.10

    Yuan, Z.Y., Zhang, B.L., Raxworthy, C.J., Weisrock, D.W., Hime, P.M., Jin, J.Q., Lemmon, E.M., Lemmon, A.R., Holland, S.D., Kortyna, M.L., Zhou, W.W., Peng, M.S., Che, J. & Prendini, E. (2019b) Natatanuran frogs used the Indian Plate to step-stone disperse and radiate across the Indian Ocean. National Science Review, 6, 10–14.

    https://doi.org/10.1093/nsr/nwy092

    Zhang, M., Han, F., Ye, J., Ni, Q., Li, Y., Yao, Y. &Xu, H. (2017) The entire mitochondrial genome of Vietnam warty newt Paramesotriton deloustali (Salamandridae: Paramesotriton) with a new distribution record from China. Conservation Genetics Resources, 10, 1–4.

    https://doi.org/10.1007/s12686-017-0804-3