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Published: 2020-12-15

Towards completion of the species inventory of small-sized leaf-tailed geckos: two new species of Uroplatus from northern Madagascar

Zoologie et Biodiversité Animale, Université d’Antananarivo, BP 906, Antananarivo, 101 Madagascar
Zoologische Staatssammlung München (ZSM-SNSB), Münchhausenstraße 21, 81247 München, Germany
Association Vahatra, lot VA 38 LB Ter A, Ambohidempona-Tsiadana, Antananarivo 101, Madagascar
Zoologie et Biodiversité Animale, Université d’Antananarivo, BP 906, Antananarivo, 101 Madagascar
Department of Biogeography and Global Change, Museo Nacional de Ciencias Naturales—CSIC. Calle José Gutierrez Abascal 2, 28006, Madrid, Spain.
Zoologische Staatssammlung München (ZSM-SNSB), Münchhausenstraße 21, 81247 München, Germany Centrum für Naturkunde, Universität Hamburg, Martin-Luther-King-Platz 3, 20146 Hamburg, Germany
Braunschweig University of Technology, Zoological Institute, Mendelssohnstraße 4, 38106 Braunschweig, Germany
Zoologische Staatssammlung München (ZSM-SNSB), Münchhausenstraße 21, 81247 München, Germany Braunschweig University of Technology, Zoological Institute, Mendelssohnstraße 4, 38106 Braunschweig, Germany Institute for Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24–25, 14476, Potsdam, Germany
Reptilia Squamata Gekkonidae Uroplatus fangorn sp. nov. Uroplatus fivehy sp. nov.


The northern part of Madagascar is well known for its high species diversity and endemism. Exceptional species richness is related to the existence of large forest blocks and mountain complexes. These areas shelter a diverse variety of habitats occupied by a wide diversity of species, including leaf-tailed geckos of the genus Uroplatus. Based on morphological and molecular evidence, we here formally name two evolutionary lineages as new species that previously had been considered as candidate species (Uroplatus spp. Ca3 and Ca4), both small-sized species of the Uroplatus ebenaui group. Genetically, both new species are related to U. finiavana with a genetic divergence (uncorrected pairwise distance) of 10.3–12.8% in a fragment of the mitochondrial 16S rRNA gene, and separated from each other by 10.3–10.7%. Uroplatus fangorn sp. nov. is described from the Marojejy massif and is also known from Andrevorevo, Lohanandroranga and Sorata; it is similar to U. ebenaui and U. fetsy but is distinguishable by its rather short tail and only partially black pigmented oral mucosa. Uroplatus fivehy sp. nov. is described from the Sorata Massif and is wider-ranging, occurring in an area from Marotandrano and Makira to Sorata, comprising Marojejy, Anjanaharibe-Sud, Ankitsika and Betaolana; it is morphologically similar to U. finiavana but distinguishable by tail size and shape. Both newly described species are found in rainforest from mid to high elevation and range respectively from 840–1417 m for U. fivehy sp nov. and 1300–1800 m for U. fangorn sp. nov. According to their respective geographical distributions, we propose to classify the two new species as Vulnerable under the IUCN Red List criteria, due to their occurrence in some forests outside of the protected area network plus the continuing decline of forest patches in the north of Madagascar.



  1. Avise, J.C. & Ball, R.M. (1990) Principles of genealogical concordance in species concepts and biological taxonomy. In: Futuyma, D. & Antonovics, J. (Eds.), Oxford surveys in evolutionary biology. Oxford Univ. Press, Oxford, pp. 45–67.

    Avise, J.C. & Wollenberg, K. (1997) Phylogenetics and the origin of species. Proceedings of the National Academy of Sciences of the United States of America, 94, 7748–7755.

    Brown, J.L., Sillero, N., Glaw, F., Bora, P., Vieites, D.R. & Vences, M. (2016) Spatial biodiversity patterns of Madagascar’s amphibians and reptiles. PLoS ONE, 11, e0144076.

    Bruford, M.W., Hanotte, O., Brookfield, J.F.Y. & Burke, T. (1992) Single-locus and multilocus DNA fingerprint. In: Hoelzel, A.R. (Ed.), Molecular Genetic Analysis of Populations: A Practical Approach. IRL Press, Oxford, 225–270.

    de Queiroz, K. (1998) The general lineage concept of species, species criteria, and the process of speciation. In: Howard, D.J. & Berlocher, S.H. (Eds.), Endless Forms. Species and Speciation. Oxford University Press, Oxford, pp. 49–89

    de Queiroz, K. (2007) Species concepts and species delimitation. Systematic Biology, 56, 879–886.

    Flieger, V. (2013) How trees behave–Or do they? Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature, 32, 21–33.

    Greenbaum, E., Bauer, A.M., Jackman, T.R., Vences, M. & Glaw, F. (2007) A phylogeny of the enigmatic Madagascan geckos of the genus Uroplatus (Squamata: Gekkonidae). Zootaxa, 1493 (1), 41–51.

    IUCN. (2012) IUCN Red List Categories and Criteria: Version 3.1. IUCN, Gland and Cambridge.

    Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33, 1870–1874.

    Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. & Calcott, B. (2016) PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution, 34, 772–773.

    Librado, P. & Rozas, J. (2009) DnaSP. Version 5. A software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25, 1451–1452.

    Moat, J. & Smith, P. (Eds.) (2007) Atlas of the vegetation of Madagascar. Kew Publishing, Kew, 124 pp.

    Padial, J.M., Miralles, A., De la Riva, I. & Vences, M. (2010) The integrative future of taxonomy. Frontiers in Zoology, 7, e16.

    Rakotoarison, A., Crottini, A., Müller, J., Rödel, M.-O., Glaw, F. & Vences, M. (2015) Revision and phylogeny of narrow-mouthed treefrogs (Cophyla) from northern Madagascar: integration of molecular, osteological, and bioacoustic data reveals three new species. Zootaxa, 3937 (1), 61–89.

    Rakotoarison, A., Scherz, M.D., Bletz, M.C., Razafindraibe, J.H., Glaw, F. & Vences, M. (2019) Diversity, elevational variation, and phylogenetic origin of stump-toed frogs (Microhylidae: Cophylinae: Stumpffia) on the Marojejy massif, northern Madagascar. Salamandra, 55, 115–123.

    Raselimanana, A.P., Raxworthy, C.J. & Nussbaum, R.A. (2000) Herpetofaunal species diversity and elevational distribution within the Parc National de Marojejy, Madagascar. In: Goodman, S.M. (Ed.), A floral and faunal inventory of the Parc National de Marojejy, Madagascar: With reference to elevational variation. Fieldiana, Zoology, New Series, 97, 157–174.

    Ratsoavina, F.M., Gehring, P.S., Scherz, M.D., Vieites, D.R., Glaw, F. & Vences, M. (2017) Two new species of leaf-tailed geckos (Uroplatus) from the Tsaratanana mountain massif in northern Madagascar. Zootaxa, 4347 (3), 446–464.

    Ratsoavina, F.M., Louis, E.E. Jr., Crottini, A., Randrianiaina, R.D., Glaw, F. & Vences, M. (2011) A new leaf tailed gecko species from northern Madagascar with a preliminary assessment of molecular and morphological variability in the Uroplatus ebenaui group. Zootaxa, 3022 (1), 39–57.

    Ratsoavina, F.M., Raminosoa, N.R., Louis, E.E. Jr., Raselimanana, A.P., Glaw, F. & Vences, M. (2013) An overview of Madagascar’s leaf tailed geckos (genus Uroplatus): species boundaries, candidate species and review of geographical distribution based on molecular data. Salamandra, 49, 115–148.

    Ratsoavina, F.M., Ranjanaharisoa, F.A., Glaw, F., Raselimanana, A.P., Miralles, A. & Vences, M. (2015) A new leaf-tailed gecko of the Uroplatus ebenaui group (Squamata: Gekkonidae) from Madagascar’s central eastern rainforests. Zootaxa, 4006 (1), 143–160.

    Ratsoavina, F.M., Raselimanana, A.P., Scherz, M.D., Rakotoarison, A., Razafindraibe, J.H., Glaw, F. & Vences, M. (2019a) Finaritra! A new leaf-tailed gecko (Uroplatus) species from Marojejy National Park in north-eastern Madagascar. Zootaxa, 4545 (4), 563–577.

    Ratsoavina, F.M., Scherz, M.D., Tolley, K.A., Raselimanana, A.P., Glaw, F. & Vences, M. (2019b) A new species of Uroplatus (Gekkonidae) from Ankarana National Park, Madagascar, of remarkably high genetic divergence. Zootaxa, 4683 (1), 84–96.

    Ratsoavina, F.M., Vences, M. & Louis, E.E. Jr. (2012) Phylogeny and phylogeography of the Malagasy leaf-tailed geckos in the Uroplatus ebenaui group. African Journal of Herpetology, 61, 143–158.

    Raxworthy, C.J., Pearson, R.G., Zimkus, B.M., Reddy, S., Deo, A.J., Nussbaum, R.A. & Ingram, C.M. (2008) Continental speciation in the tropics: contrasting biogeographic patterns of divergence in the Uroplatus leaf-tailed gecko radiation of Madagascar. Journal of Zoology, 275, 423–440.

    Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542.

    Salzburger, W., Ewing, G.B. & Von Haeseler, A. (2011) The performance of phylogenetic algorithms in estimating haplotype genealogies with migration. Molecular Ecology, 20, 1952–1963.

    Scherz, M.D., Vences, M., Borrell, J., Ball, L., Nomenjanahary, D.H., Parker, D., Rakotondratsima, M., Razafimandimby, E., Starnes, T., Rabearivony, J. & Glaw, F. (2017) A new frog species of the subgenus Asperomantis (Anura: Mantellidae: Gephyromantis) from the Bealanana District of northern Madagascar. Zoosystematics and Evolution, 93, 451–466.

    Shen, X.X., Liang, D. & Zhang, P. (2012) The development of three long universal nuclear protein-coding locus markers and their application to osteichthyan phylogenetics with nested PCR. PLoS ONE, 7, e39256.

    Speybroeck, J., Beukema, W., Dufresnes, C., Fritz, U., Jablonski, D., Lymberakis, P., Martinez-Solano, I., Razzettis, E., Vamberger, M., Vences, M., Vörös, J. & Crochet, P.-A. (2020) Species list of the European herpetofauna—2020 update by the Taxonomic Committee of the Societas Europaea Herpetologica. Amphibia-Reptilia, 41, 139–189.

    Stephens, M., Smith, N.J. & Donnelly, P. (2001) A new statistical method for haplotype reconstruction from population data. American Journal of Human Genetics, 68, 978–989.

    Wollenberg, K.C., Vieites, D.R., Glaw, F. & Vences, M. (2011) Speciation in little: the role of range and body size in the diversification of Malagasy mantellid frogs. BMC Evolutionary Biology, 11, 217.