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Published: 2020-08-26

A new species Cyrtodactytlus Gray (Squamata: Gekkonidae) from western Thailand and the phylogenetic placement of C. inthanon and C. doisuthep

Research Center in Bioresources for Agriculture, Industry and Medicine, Department of Biology, Faculty of Science, Chiang Mai University, 239 Huay Kaew Rd. Tambon Su Thep, Chiang Mai 50200, Thailand.
Division of Fishery, School of Agriculture and Natural Resources, University of Phayao, Phayao, Thailand.
Division of Fishery, School of Agriculture and Natural Resources, University of Phayao, Phayao, Thailand.
Research Center in Bioresources for Agriculture, Industry and Medicine, Department of Biology, Faculty of Science, Chiang Mai University, 239 Huay Kaew Rd. Tambon Su Thep, Chiang Mai 50200, Thailand.
Zoological Museum, Moscow State University, B. Nikitskaya ul. 2, Moscow 125009, Russia.
Herpetology Laboratory, Department of Biology, La Sierra University, 4500 Riverwalk Parkway, Riverside, California 92515, USA.
Faculty of Biology, Department of Vertebrate Zoology, Moscow State University, Moscow, Russia. Laboratory of Tropical Ecology, Joint Russian-Vietnamese Tropical Research and Technological Center, Hanoi, Vietnam
Reptilia Integrative taxonomy Indochina Tenasserim Mountains Bent-toed gecko


A new species of Cyrtodactylus from Tak Province, Thailand, Cyrtodactylus amphipetraeus sp. nov., is described using an integrative taxonomic analysis based on morphology, color pattern, and the mitochondrial gene NADH dehydrogenase subunit 2 (ND2). The phylogenetic analyses place the new species within the C. sinyineensis group which was previously thought to be endemic to the Salween Basin in southern Myanmar. The phylogeny also places C. inthanon in the C. sinyneensis group which is expanded herein to also include the group’s sister species C. doisuthep. Along with C. amphipetraeus sp. nov., these are the first three species of the C. sinyineensis group to be found outside of Myanmar east of the Tenasserim Mountains. The Tenasserim Mountain region is discussed as an area of cladogeneic turnover.



  1. Barraclough, T.G., Birky, C.W. Jr. & Burt, A. (2003) Diversification in sexual and asexual organisms. Evolution, 57, 2166–2172.

    Bauer, A.M., Kunya, K., Sumontha, M., Niyomwan, P., Pauwels, O.S.G., Chanhome, L. & Kunya, T. (2010) Cyrtodactylus dumnuii (Squamata: Gekkonidae), a new cave-dwelling gecko from Chiang Mai Province, Thailand. Zootaxa, 2570 (1), 41–50.

    Bauer, A.M., Kunya, K., Sumontha, M., Niyomwan, P., Panitvong, N., Pauwels, O.S.G., Chanhome, L. & Kunya, T. (2009) Cyrtodactylus erythrops (Squamata: Gekkonidae), a new cave-dwelling gecko from Mae Hong Son Province, Thailand. Zootaxa, 2124 (1), 51–62.

    Chan-ard, Tanya & Makchai, S. (2011) A New Insular Species of Cyrtodactylus Gray, 1827 (Squamata, Gekkonidae), from the Surin Islands, Phang-nga Province, Southern Thailand. The Thailand Natural History Museum Journal, 5, 7­–15.

    Coyne, J.A. & Orr, H.A. (1998) The evolutionary genetics of speciation. Philosophical Transactions of the Royal Society of London B, 353, 287–305.

    De Queiroz, K. (2007) Species concepts and species delimitation. Systematic Biology, 56, 879–886.

    Drummond, A.J., Suchard, M.A., Xie, D. & Rambaut, A. (2012) Bayesian Phylogenetics with BEAUti and BEAST 1.7. Molecular Biology and Evolution, 29, 1969–1973.

    Fontaneto, D., Herniou, E.A., Boschetti, C., Caprioli, M., Melone, G., Ricci, C. & Barrenclough, T.G. (2007) Independently evolving species in asexual bdelloid rotifers. PLoS Biology, 5, e87.

    Grismer, L.L., Wood Jr., P.L., Grismer, M.S., Quah, E.S.H., Thura, M.K., Oaks, J.R., Lin, A. & Lim, D.Y. (2020a) Integrative taxonomic and geographic variation analyses in Cyrtodactylus aequalis (Squamata: Gekkonidae) from southern Myanmar (Burma): one species, two different stories. Israel Journal of Ecology & Evolution. [in press]

    Grismer, L.L., Wood Jr., P.L., Quah, E.S.H., Grismer, M.S., Thura, M.K., Oaks, J.R. & Lin, A. (2020b) Two mew species of Cyrtodactylus Gray, 1827 (Squamata: gekkonidae) from a karstic archipelago in the Salween Basin of southern Myanmar (Burma). Zootaxa, 4718, 151–183.

    Grismer, L.L., Wood Jr., P.L., Thura, M.K., Quah, E.S.H., Grismer, M.S., Murdoch, M.L., Espinoza, R.E. & Lin, A. (2018b) A new Cyrtodactylus Gray (Squamata, Gekkonidae) from the Shan Hills and the biogeography of Bent-toed Geckos from eastern Myanmar. Zootaxa, 4446 (4), 477–500.

    Grismer, L.L., Wood Jr., P.L., Thura, M.K., Zin, T., Quah, E.S.H., Murdoch, M.L., Grismer, M.S., Lin, A., Kyaw, H. & Ngwe, L. (2018a) Twelve new species of Cyrtodactylus Gray (Squamata: Gekkonidae) from isolated limestone habitats in east-central and southern Myanmar demonstrate high localized diversity and unprecedented microendemism. Zoological Journal of the Linnean Society, 182, 862–959.

    Hillis, D.M. (2019) Species delimitation in herpetology. Journal of Herpetology, 53, 3–12.

    Hillis, D.M., Moritz, C., Mable, B.K. & Graur, D. (1996) Molecular Systematics. 2nd Edition. Sinauer Associates, Inc., Sunderland, Massachusetts, 655 pp.

    Hoang, D.T., Chernomor, O., von Haeseler, A., Minh, B.Q. & Vinh, L.S. (2018) UFBoot2: Improving the ultrafast bootstrap approximation. Molecular Biology and Evolution, 35, 518–522.

    Huelsenbeck, J.P., Ronquist, F., Nielsen, R. & Bollback, J.P. (2001) Bayesian Inference of Phylogeny and Its Impact on Evolutionary Biology. Science, 294, 2310–2314.

    Jombart, T., Devillard, S. & Balloux, F. (2010) Discriminant analysis of principal components: a new method for the analysis of genetically structured populations. BMC Genetics, 11, 94.

    Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K., von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods, 14, 587.

    Katoh, M. & Kuma, M. (2002) MAFTT: a novel method for rapid sequence alignment based on fast Fourier transform. Nucleic Acids Research, 30, 3059–3066.

    Knowles, L.L. & Carstens, B.C. (2007) Delimiting species without monophyletic gene trees. Systematic Biology, 56, 887–895.

    Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33, 1870–1874.

    Kunya, K., Panmongkol, A., Pauwels, O.S.G., Sumontha, M., Meewasana, J., Bunkhwamdi, W. & Dangsri, S. (2014) A new forest-dwelling Bent-toed Gecko (Squamata: Gekkonidae: Cyrtodactylus) from Doi Suthep, Chiang Mai Province, northern Thailand. Zootaxa, 3811 (2), 251–261.

    Kunya, K., Sumontha, M., Panitvong, N., Dongkumfu, W., Sirisamphan, T. & Pauwels, O.S.G. (2015) A new forest-dwelling Bent-toed Gecko (Squamata: Gekkonidae: Cyrtodactylus) from Doi Inthanon, Chiang Mai Province, northern Thailand. Zootaxa, 3905 (4), 573–584.

    Leaché, A.D., Koo, M.S., Spencer, C.L., Papenfuss, T.J., Fisher, R.N. & McGuire, J.A. (2009) Quantifying ecological, morphological, and genetic variation to delimit species in the coast horned lizard species complex (Phrynosoma). Proceedings of the National Academy of Sciences, 106, 12418–12423.

    Macey, J.R., Larson, A., Ananjeva, N.B. & Papenfuss, T.J. (1997) Evolutionary shifts in three major structural features of the mitochondrial genome among iguanian lizards. Journal of Molecular Evolution, 44, 660–674.

    Maddison, W.P. & Maddison, D.R. (2015) Mesquite: a modular system for evolutionary analysis. Version 3.04. Available from: (accessed 29 July 2020)

    Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (GCE), New Orleans, Louisiana, 14 November 2010, pp. 1–8.

    Minh, Q., Nguyen, M.A.T. & von Haeseler, A. (2013) Ultrafast approximation for phylogenetic bootstrap. Molecular Biology and Evolution, 30, 1188–1195.

    Murdoch, M.L., Grismer, L.L. Wood Jr., P.L., Neang, T., Poyarkov, N.A., Ngo, V.T., Nazarov, R.A., Aowphol, A., Pauwels, O.S.G., Nguyen, H.N. & Grismer, J.L. (2019) Six new species of the Cyrtodactylus intermedius complex (Squamata: Gekkonidae) from the Cardamom Mountains and associated highlands of Southeast Asia. Zootaxa, 4554, 1–62.

    Murphy, R.W., Crawford, A.J., Bauer, A.M., Che, J., Donnellan, S.C., Fritz, U., Haddad, C.F.B., Nagy, Z.T., Poyarkov, N.A., Vences, M., Wang, W.-Z. & Zhang, Y. (2013) Cold Code: the global initiative to DNA barcode amphibians and nonavian reptiles. Molecular Ecology Resources, 13, 161–167.

    Nazarov, R., Poyarkov, N.A., Orlov, N.L., Phung, T.M., Nguyen, T.T., Hoang, D.M. & Ziegler, T. (2012) Two new cryptic species of the Cyrtodactylus irregularis complex (Squamata: Gekkonidae) from southern Vietnam. Zootaxa, 3302 (1), 1–24.

    Nazarov, R.A., Poyarkov, N.A., Orlov, N.L., Nguyen, N.S., Milto, K.D., Martynov, A.A., Konstantinov, E.L. & Chulisov, A.S. (2014) A review of Cyrtodactylus (Reptilia: Sauria: Geckonidae) fauna of Laos with description of four new species. Proceedings of the Zoological Institute RAS, 318 (4), 391–423.

    Nazarov, R.A., Pauwels, O.S.G., Konstantinov, E.L., Chulisov, A.S., Orlov, N.L. & Poyarkov, N.A. (2018) A new karst-dwelling bent-toed gecko (Squamata: Gekkonidae: Cyrtodactylus) from Xiangkhoang Province, northeastern Laos. Zoological Research, 38, 3, 197–213.

    Nguyen, L.-T., Schmidt, H.A., von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: A fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Molecular Biology and Evolution, 32, 268–274.

    Pauwels, O.S.G. & Sumontha, M. (2014) Cyrtodactylus samroiyot, a new limestone-dwelling Bent-toed Gecko (Squamata: Gekkonidae) from Prachuap Khiri Khan Province, peninsular Thailand. Zootaxa, 3755 (6), 573–583.

    Pauwels, O.S.G., Sumontha, M. & Bauer, A.M. (2016) A new Bent-toed Gecko (Squamata: Gekkonidae: Cyrtodactylus) from Phetchaburi Province, Thailand. Zootaxa, 4088 (3), 409–419.

    Pauwels, O.S.G., Sumontha, M., Keeratikiat, K. & Phanamphon, E. (2014) Cyrtodactylus kunyai (Squamata: Gekkonidae), a new cave-dwelling Bent-toed Gecko from Loei Province, northeastern Thailand. Zootaxa, 3821 (2), 253–264.

    Pauwels, O.S.G., Sumontha, M., Panitvong, N. & Varaguttanonda, V. (2014) Cyrtodactylus khelangensis, a new cave-dwelling Bent-toed Gecko (Squamata: Gekkonidae) from Lampang Province, northern Thailand. Zootaxa, 3755 (6), 584–594.

    R Core Team (2018) R: A language and environment for statistical computing. R Foundation for Statistical Computing. Vienna. Available from: (accessed 29 July 2020)

    Rambaut, A., Suchard, M.A., Xie, D. & Drummond, A.J. (2014) Tracer. Version 1.6. Available from: (accessed 29 July 2020)

    Sabaj, M.H. (2016) Standard symbolic codes for institutional resource collections in herpetology and ichthyology: an Online Reference. Version 6.5. 16 August 2016. American Society of Ichthyologists and Herpetologists, Washington, D.C. Available from: (accessed 29 July 2020)

    Shaffer, H.B., Gidis, M., McCartney-Melstad, E., Neal, K.M., Oyamaguchi, H.M., Tellez, M. & Toffelmeir, E.M. (2015) Conservation genetics and genomics of amphibians and reptiles. Annual Review of Animal Biosciensces, 3, 23.1–23.26.

    Smith, A.M., Poyarkov, N.A. & Hebert, P.D.N. (2008) CO1 DNA barcoding amphibians: take the chance, meet the challenge. Molecular Ecology Notes, 8, 235–246.

    Sumontha, M., Pauwels, O.S.G., Panitvong, N., Kunya, K. & Grismer, L.L. (2015) A new lowland forest Bent-toed Gecko (Squamata: Gekkonidae: Cyrtodactylus) from Ranong Province, peninsular Thailand. Zootaxa, 3911 (1), 106–118.

    Sumontha, M., Pauwels, O.S.G., Suwannakarn, N., Nutatheera, T. & Sodob, W. (2014) Cyrtodactylus wangkulangkulae (Squamata: Gekkonidae), a new Bent-toed Gecko from Satun Province, southern Thailand. Zootaxa, 3821 (1), 116–124.

    Trifinopoulos, J., Nguyen, L.-T., von Haeseler, A. & Minh, B.Q. (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research, 44, W232–W235.

    Uetz, P., Freed, P. & Hošek, J. (Eds.) (2020) The Reptile Database. Available from: (accessed 29 July 2020)

    Wilcox, T.P., Zwickl, D.J., Heath, T.A. & Hillis, D.M. (2002) Phylogenetic relationships of the Dwarf Boas and a comparison of Bayesian and bootstrap measures of phylogenetic support. Molecular Phylogenetics and Evolution, 25, 361–371.