Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2020-03-27
Page range: 127–140
Abstract views: 68
PDF downloaded: 1

Genetic and morphologic identities of hydrozoans (Cnidaria, Hydrozoa) from reef patches of Santa Marta, Colombian Caribbean

Universidad Jorge Tadeo Lozano. Department of Biological and Environmental Sciences. Cra. 2 #11-68, El Rodadero, Santa Marta, Colombia.
Universidad Jorge Tadeo Lozano. Department of Biological and Environmental Sciences. Cra. 2 #11-68, El Rodadero, Santa Marta, Colombia.
Universidad Jorge Tadeo Lozano. Department of Biological and Environmental Sciences. Cra. 2 #11-68, El Rodadero, Santa Marta, Colombia.
Universidad Jorge Tadeo Lozano. Department of Biological and Environmental Sciences. Cra. 2 #11-68, El Rodadero, Santa Marta, Colombia.
Universidad Jorge Tadeo Lozano. Department of Biological and Environmental Sciences. Cra. 2 #11-68, El Rodadero, Santa Marta, Colombia.
Coelenterata Mitochondrial DNA 16S rRNA gene genetic identification

Abstract

The morphological and genetic identification of hydrozoans collected in the reef patches of Santa Marta, Colombia was carried out. This study allows to present two new records of hydroids species for the Colombian Caribbean: Halopteris alternata and Dentitheca dendritica. A total of 11 species and 1 genus were found using morphological and genetic identification with partial sequences of the mitochondrial 16S rRNA gene. The order Leptothecata was the most abundant represented by 9 families: Aglaopheniidae, Clytiidae, Haleciidae, Halopterididae, Kirchenpaueriidae, Plumulariidae, Sertularellidae, Sertulariidae and Thyroscyphidae, while the order Anthoathecata was represented by 2 families: Eudendriidae and Pennariidae. Despite the lack of studies on this group of organisms in the country, the use of the 16S rRNA gene proved to be very useful to provide complementary evidence in our understanding of the biological diversity of hydrozoans in Colombia.

 

References

  1. Allman, G.J. (1877) Report on the Hydroida collected during the exploration of the Gulf Stream by L.F. de Pourtalès, assistant United States Coast Survey. Memoirs of the Museum of Comparative Zoölogy at Harvard College, 5 (2), 1–66.

    https://doi.org/10.5962/bhl.title.15852

    Allman, G.J. (1885) Description of Australian, Cape and other Hydroids, mostly new, from the collection of Miss H. Gatty. Journal of the Linnean Society, 19, 132–161.

    Allman, G.J. (1888) Report on the Hydroida dredged by H.M.S. Challenger during the years 1873–76. Part II. The Tubularinae, Corymorphinae, Campanularinae, Sertularinae, and Thalamophora. Report on the Scientific Results of the Voyage of H.M.S. Challenger during the years 1873–76, Zoology, 23, 1–90.

    Ansín Agís, J., Ramil, F. & Vervoort, W. (2001) Atlantic Leptolida (Hydrozoa, Cnidaria) of the families Aglaopheniidae, Halopterididae, Kirchenpaueriidae and Plumulariidae collected during the CANCAP and Mauritania-II expeditions of the National Museum of Natural History, Leiden, the Netherlands. Zoologische Verhandelingen, 333, 1–268.

    Avise, J.C. (1994) Molecular Markers, Natural History and Evolution: Natural History and Evolution. Springer Science and Business Media, Boston, Massachusetts, 511 pp.

    https://doi.org/10.1007/978-1-4615-2381-9

    Bandel, K. & Wedler, E. (1987) Hydroid, amphineuran and gastropod zonation in the littoral of the Caribbean Sea, Colombia. Senckenbergiana Maritima, 19 (1/2), 1–129.

    Bedot, M. (1914) Nouvelles notes sur les hydroïdes de Roscoff. Archives de Zoologie Expérimentale et Générale, 54, 79–98.

    Blackstone, N.W. & Buss, L.W. (1991) Shape variation in hydractiniid hydroids. The Biological Bulletin, 180 (3), 394–405.

    https://doi.org/10.2307/1542340

    Brandt, J.F. (1835) Prodromus descriptionis animalium ab H. Mertensio in orbis terrarum circumnavigatione observatorum. Fascic. I., Polypos, Acalephas Discophoras et Siphonophoras, nec non Echinodermata continens / auctore, Johanne Friderico Brandt. Recueil Actes des séances publiques de l’Acadadémie impériale des Science de St. Pétersbourg, 1834, 201–275

    https://doi.org/10.5962/bhl.title.10196

    Busk, G. (1852) Appendix IV. An account of the Polyzoa, and sertularian zoophytes, collected in the voyage of the Rattlesnake, on the coasts of Australia and the Louisiade Archipelago, &c. In: Macgillivray, J. (Ed.), Narrative of the voyage of H.M.S. Rattlesnake, commanded by the late Captain Owen Stanley, R.N., F.R.S. &c., during the years 1846–1850. Vol. 1. T. & W. Boone, London, pp. 343–402.

    Cairns, S.D., Calder, D.R., Brinckmann-Voss, A., Castro, C.B., Fautin, D.G., Pugh, P.R., Mills, C.E., Jaap, W.C., Arai, M.N., Haddock, S.H.D. & Opresko, D.M. (2002) Common and scientific names of aquatic invertebrates from the United States and Canada: Cnidaria and Ctenophora. Second Edition. American Fisheries Society Special Publication, 28, 1–115.

    Calder, D.R. (1997) Shallow-water hydroids of Bermuda: superfamily Plumularioidea. Royal Ontario Museum, Life Sciences Contributions, 161, 1–85.

    Calder, D.R. & Kirkendale, L. (2005) Hydroids (Cnidaria, Hydrozoa) from shallow-water environments along the Caribbean coast of Panama. Caribbean Journal of Science, 41, 476–491.

    Calder, D.R. & Cairns, S.D. (2009) Hydroids (Cnidaria: Hydrozoa) of the Gulf of Mexico. In: Felder, D.L. & Camp, D.K. (Eds.), Gulf of Mexico. Origin, waters, and biota. Vol. 1. Biodiversity. Texas A&M University Press, College Station, Texas, pp. 381–394.

    Calder, D.R., Vervoort, W. & Hochberg, F.G. (2009) Lectotype designations of new species of hydroids (Cnidaria, Hydrozoa), described by CM Fraser, from Allan Hancock Pacific and Caribbean Sea Expeditions. Zoologische Mededelingen, 83 (32), 919–1058.

    Calder, D.R. (2013) Some shallow-water hydroids (Cnidaria: Hydrozoa) from the central east coast of Florida, USA. Zootaxa, 3648 (1), 1–72.

    https://doi.org/10.11646/zootaxa.3648.1.1

    Cartwright, P., Evans, N.M., Dunn, C.W., Marques, A.C., Miglietta, M.P., Schuchert, P. & Collins, A.G. (2008) Phylogenetics of Hydroidolina (Hydrozoa: Cnidaria). Journal of the Marine Biological Association of the UK, 88 (8), 1663–1672.

    https://doi.org/10.1017/S0025315408002257

    Clarke, S.F. (1882) New and interesting hydroids from Chesapeake Bay. Memoirs of the Boston Society of Natural History, 3, 135–142.

    Collins, A.G. (2000) Towards understanding the phylogenetic history of Hydrozoa: hypothesis testing with 18S gene sequence data. Scientia Marina, 64 (Suplemento 1), 5–22.

    https://doi.org/10.3989/scimar.2000.64s15

    Collins, A.G., Winkelmann, S., Hadrys, H. & Schierwater, B. (2005) Phylogeny of Capitata and Corynidae (Cnidaria, Hydrozoa) in light of mitochondrial 16S rDNA data. Zoologica Scripta, 34 (1), 91–99.

    https://doi.org/10.1111/j.1463-6409.2005.00172.x

    Cornelius, P.F.S. (1992) Medusa loss in leptolid Hydrozoa (Cnidaria), hydroid rafting, and abbreviated life-cycles among their remote-island faunae: an interim review. Scientia Marina, 56, 245–261.

    Costello, M.J., Coll, M., Danovaro, R., Halpin, P., Ojaveer, H. & Miloslavich, P. (2010) A census of marine biodiversity knowledge, resources, and future challenges. PLoS One, 5 (8), e12110.

    https://doi.org/10.1371/journal.pone.0012110

    Cunha, A.F., Collins, A.G. & Marques, A.C. (2017) Phylogenetic relationships of Proboscoida Broch, 1910 (Cnidaria, Hydrozoa): Are traditional morphological diagnostic characters relevant for the delimitation of lineages at the species, genus, and family levels? Molecular Phylogenetics and Evolution, 106, 118–135.

    https://doi.org/10.1016/j.ympev.2016.09.012

    Cunningham, C.W. & Buss, L.W. (1993) Molecular evidence for multiple episodes of paedomorphosis in the family Hydractiniidae. Biochemical Systematics and Ecology, 21 (1), 57–69.

    https://doi.org/10.1016/0305-1978(93)90009-G

    Daly, M., Brugler, M.R., Cartwright, P., Collins, A.G., Dawson, M.N., Fautin, D.G. & Stake, J.L. (2007) The phylum Cnidaria: a review of phylogenetic patterns and diversity 300 years after Linnaeus. Zootaxa, 1668 (1), 127–182.

    https://doi.org/10.11646/zootaxa.1668.1.11

    Di Camillo, C.G., Bo, M., Puce, S. & Bavestrello, G. (2010) Association between Dentitheca habereri (Cnidaria: Hydrozoa) and two zoanthids. Italian Journal of Zoology, 77, 81–91.

    https://doi.org/10.1080/11250000902740962

    Eschscholtz, F. (1829) System der Acalephen. Eine ausfürliche Beschreibung aller medusenartigen Strahltiere. Ferdinand Dümmler, Berlin, 190 pp.

    https://doi.org/10.5962/bhl.title.10139

    Flórez, L. (1983) Inventario preliminar de la fauna hidroide de la Bahía de Cartagena y áreas adyacentes. Boletín del Museo del Mar, 11, 112–140.

    Galea, H.R. (2008) On a collection of shallow-water hydroids (Cnidaria: Hydrozoa) from Guadeloupe and Les Saintes, French Lesser Antilles. Zootaxa, 1878 (1), 1–54.

    https://doi.org/10.11646/zootaxa.1878.1.1

    Galea, H.R. (2010) Additional shallow-water thecate hydroids (Cnidaria: Hydrozoa) from Guadeloupe and Les Saintes, French Lesser Antilles. Zootaxa, 2570 (1), 1–40.

    https://doi.org/10.11646/zootaxa.2570.1.1

    Galea, H.R., Ferry, R. & Bertot, J.M. (2012) Medusoids in the life cycle of Dentitheca dendritica (Nutting, 1900) and Nemalecium gracile sp. nov. (Cnidaria: Hydrozoa). Zootaxa, 3527 (1), 43–54.

    https://doi.org/10.11646/zootaxa.3527.1.3

    Gomez, L. & Dawson, M. (2017) Species richness of jellyfishes (Scyphozoa: Discomedusae) in the Tropical Eastern Pacific: missed taxa, molecules, and morphology match in a biodiversity hotspot. Invertebrate Systematics, 31 (5), 635–663.

    https://doi.org/10.1071/IS16055

    Hall, T. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.

    Heller, C. (1868) Die Zoophyten und Echinodermen des Adriatischen Meeres. Verhandlungen der Kaiserlich-Königlichen Zoologisch-Botanischen Gesellschaft in Wien, 18, 1–88.

    https://doi.org/10.5962/bhl.title.11393

    Hirohito, The Showa Emperor (1995) The hydroids of Sagami Bay. Part II. Thecata. Biological Laboratory, Imperial Household, Tokyo, 355 pp.

    Humann, P. (1992) Reef creature identification. Florida Caribbean Bahamas. New World Publications, Jacksonville, Florida, 344 pp.

    Humann, P. & DeLoach, N. (2002) Reef creature identification. Florida Caribbean Bahamas. Second Edition. New World Publications, Jacksonville, Florida, 447 pp.

    Jones, T. (2002) Biology and ecology of athecate hydroids in Biscayne Bay, Florida: potential use as bioindicators. Ph.D. Dissertation, University of Miami, Coral Gables, 287 pp.

    Kirkendale, L. & Calder, D. (2003) Hydroids (Cnidaria: Hydrozoa) from Guam and the Commonwealth of the Northern Marianas Islands (CNMI). Micronesica, 35–36, 159–188.

    Kumar, S., Stecher, G. & Koichiro, T. (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0. Molecular Biology and Evolution, 33 (7), 1870–1874.

    https://doi.org/10.1093/molbev/msw054

    Leclére, L., Schuchert, P & Manuel, M. (2007) Phylogeny of the Plumularioidea (Hydrozoa, Leptothecata): evolution of colonial organization and life cycle. Zoologica Scripta, 36 (4), 371–394.

    https://doi.org/10.1111/j.1463-6409.2007.00283.x

    Leclére, L., Schuchert, P., Craud, C., Couloux, A. & Manuel, M. (2009) Molecular phylogenetics of Thecata (Hydrozoa, Cnidaria) reveals long-term maintenance of life history traits despite high frequency of recent character changes. Systematic Biology, 58 (5), 509–526.

    https://doi.org/10.1093/sysbio/syp044

    Maronna, M.M., Miranda, T.P., Peña, A.L., Barbeitos, M.S. & Marques, A.C. (2016) Towards a phylogenetic classification of Leptothecata (Cnidaria, Hydrozoa). Scientific Reports, 6, 18075.0

    https://doi.org/10.1038/srep18075

    McCrady, J. (1859) Gymnopthalmata of Charleston Harbor. Proceedings of the Elliott Society of Natural History, 1, 103–221.

    Miglietta, M.P., Piraino, S., Kubota, S. & Schuchert, P. (2007) Species in the genus Turritopsis (Cnidaria, Hydrozoa): a molecular evaluation. Journal of Zoological Systematics and Evolutionary Research, 45 (1), 11–19.

    https://doi.org/10.1111/j.1439-0469.2006.00379.x

    Miglietta, M.P., Schuchert, P. & Cunningham, C.W. (2009) Reconciling genealogical and morphological species in a worldwide study of the Family Hydractiniidae (Cnidaria, Hydrozoa). Zoologica Scripta, 38 (4), 403–430.

    https://doi.org/10.1111/j.1463-6409.2008.00376.x

    Miglietta, M.P., Piraino, S., Pruski, S., Gonzalez, M.A., Castellanos-Iglesias, S., Jeronimo, S., Lawley, J.W., Maggioni, D., Martell, L., Matsumoto, Y., Moncada, A., Nagale, P., Phongphattarawat, S., Sheridan, C., Soto Angel, J.J., Sukhoputova, A. & Collin, R. (2018) An Integrative identification guide to the Hydrozoa (Cnidaria) of Bocas del Toro, Panama. Neotropical Biodiversity, 4 (1), 102–112.

    https://doi.org/10.1080/23766808.2018.1488656

    Millard, N.A.H. (1962) The Hydrozoa of the south and west coasts of South Africa. Part I. The Plumulariidae. Annals of the South African Museum, 46, 261–319.

    Miller, S.A., Dykes, D.D. & Polesky, H.F. (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research, 16 (3), 1215.

    https://doi.org/10.1093/nar/16.3.1215

    Miloslavich, P., Díaz, J.M., Klein, E., Alvarado, J.J., Díaz, C., Gobin, J., Escobar-Briones, E., Cruz-Motta, J.J., Weil, E., Cortés, J., Bastidas, A.C., Robertson, R., Zapata, F., Martín, A., Castillo, J., Kazandjian, A. & Ortiz, M. (2010) Marine biodiversity in the Caribbean: regional estimates and distribution patterns. PLoS one, 5 (8), e11916.

    https://doi.org/10.1371/journal.pone.0011916

    Miloslavich, P., Klein, E., Díaz, J.M., Hernandez, C.E., Bigatti, G., Campos, L., Artigas, F., Castillo, J., Penchaszadeh, P.E., Neill, P.E., Carranza, A., Retana, M.V., Díaz de Astarloa, J.M., Lewis, M., Yorio, P., Piriz, M.L., Rodríguez, D., Yoneshigue-Valentin, Y., Gamboa, L. & Martín, A. (2011) Marine biodiversity in the Atlantic and Pacific coasts of South America: knowledge and gaps. PLoS one, 6 (1), e14631.

    https://doi.org/10.1371/journal.pone.0014631

    Mora, C., Andrefouet, S., Costello, M.J., Kranenburg, C., Rollo, A., Veron, J., Gaston, K.J. & Myers, R.A. (2006) Ecology: Coral reefs and the global network of marine protected areas. Science, 312 (5781), 1750–1751.

    https://doi.org/10.1126/science.1125295

    Moura, C.J., Harris, D.J., Cunha, M.R. & Rogers, A.D. (2008) DNA barcoding reveals cryptic diversity in marine hydroids (Cnidaria, Hydrozoa) from coastal and deep sea environments. Zoologica Scripta, 37 (1), 93–108.

    Moura, C.J., Cunha, M.R., Porteiro, F.M. & Rogers, A.D. (2011) The use of the DNA barcode gene 16S mRNA for the clarification of taxonomic problems within the family Sertulariidae (Cnidaria, Hydrozoa). Zoologica Scripta, 40 (5), 520–537.

    https://doi.org/10.1111/j.1463-6409.2011.00489.x

    Moura, C.J. (2011) Systematics and evolution of coastal and deepwater Hydrozoa from the NE Atlantic. Universidade de Aveiro, Portugal, 330 pp.

    Nutting, C.C. (1900) American hydroids. Part I. The Plumularidae. Smithsonian Institution, United States National Museum Special Bulletin, 4 (1), 1–285.

    Oliveira, O.M.P., Araujo, E.M., Ayón, P., Cedeño-Posso, C.M., Cepeda, A.A., Córdova, P., Galea, H.R., Genzano, G.N., Haddad, M.A., Mianzan, H.W., Migotto, A.E., Miranda, L.S., Miranda, T.P., Morandini, A.C., Nagata, R.M., Nascimento, K.B., Nogueira Jr., M., Palma, S., Quiñones, J., Rodriguez, C.S., Scarabino, F., Schiariti, A., Stampar, S.N., Tronolone, V.B. & Marques, A.C. (2016) Census of Cnidaria (Medusozoa) and Ctenophora from South American marine waters, Zootaxa, 4194 (1), 001–256.

    https://doi.org/10.11646/zootaxa.4194.1.1

    Owen, R. (1843) Lectures on the comparative anatomy and physiology of the invertebrate animals: delivered at the Royal College of Surgeons, in 1843. Longman, Brown, Green, & Longmans, London, 392 pp.

    Posada, D. & Crandall, K. (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics, 14 (9), 817–818.

    https://doi.org/10.1093/bioinformatics/14.9.817

    Posada, T., Peña, A. & Navas, G. (2010) Hidrozoos de la familia Aglaopheniidae de la plataforma continental y talud superior del Caribe colombiano. Boletín de Investigaciones Marinas y Costeras, 39 (1), 67–81.

    https://doi.org/10.25268/bimc.invemar.2010.39.1.143

    Postaire, B., Aurelle, D., Bourmaud, C.A., Bruggemann, J.H. & Magalon, H. (2015) Isolation and characterization of 26 microsatellite loci from a widespread tropical hydrozoan, Macrorhynchia phoenicea (Leptothecata, Aglaopheniidae), and cross-amplification in closely related species. Biochemical Systematics and Ecology, 62, 137–141.

    https://doi.org/10.1016/j.bse.2015.08.011

    Postaire, B., Magalon, H., Bourmaud, C.A. & Bruggemann, J.H. (2016) Molecular species delimitation methods and population genetics data reveal extensive lineage diversity and cryptic species in Aglaopheniidae (Hydrozoa). Molecular Phylogenetics and Evolution, 105, 36–49.

    https://doi.org/10.1016/j.ympev.2016.08.013

    Reyes, R. & Campos, N. (1992) Macroinvertebrados colonizadores de raíces de Rhizophora mangle en la bahía de Chengue, Caribe colombiano. Anales del Instituto de Investigaciones Marinas de Punta Betín, 21, 101–116.

    https://doi.org/10.25268/bimc.invemar.1992.21.0.422

    Roberts, C.M., McClean, C.J., Veron, J.E., Hawkins, J.P., Allen, G.R., McAllister, D.E. & Werner, T.B. (2002) Marine biodiversity hotspots and conservation priorities for tropical reefs. Science, 295 (5558), 1280–1284.

    https://doi.org/10.1126/science.1067728

    Ronquist, F. & Huelsenbeck, J. (2003) MRBAYES 3.1.2: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.

    https://doi.org/10.1093/bioinformatics/btg180

    Ryland, J.S. & Gibbons, M.J. (1991) Intertidal and shallow water hydroids from Fiji. II. Plumulariidae and Aglaopheniidae. Memoirs of the Queensland Museum, 30, 525–560.

    Schuchert, P. (1997) Review of the family Halopterididae (Hydrozoa, Cnidaria). Zoologische Verhandelingen, Leiden, 309, 1–162.

    Schuchert, P. (2003) Hydroids (Cnidaria, Hydrozoa) of the Danish expedition to the Kei Islands. Steenstrupia, 27, 137–256.

    Schuchert, P. (2005) Species boundaries in the hydrozoan genus Coryne. Molecular Phylogenetics and Evolution, 36 (1), 194–199.

    https://doi.org/10.1016/j.ympev.2005.03.021

    Schuchert, P. (2008) The European athecate hydroids and their medusae (Hydrozoa, Cnidaria): Filifera Part 4. Revue Suisse de Zoologie, 115 (14), 677–757.

    https://doi.org/10.5962/bhl.part.80453

    Schuchert, P. (2014) World Hydrozoa database. Available from: http://marinespecies.org/hydrozoa (accessed 4 March 2018)

    Schuchert, P. (2016) DNA Barcoding of Hydrozoa in the collection of the Natural History Museum of Geneva. Available from: https://www.ncbi.nlm.nih.gov/nuccore/KX355433.1 (accessed 07 July 2018)

    Schroth, W., Jarms, G., Streit, B. & Schierwater, B. (2002) Speciation and phylogeography in the cosmopolitan marine moon jelly, Aurelia sp. BMC Evolutionary Biology, 2, 1.

    https://doi.org/10.1186/1471-2148-2-1

    Stechow, E. (1909) Beiträge zur Naturgeschichte Ostasiens. Herausgegeben von Dr. F. Doflein. Hydroidpolypen der japanischen Ostküste. I. Teil: Athecata und Plumularidae. Abhandlungen der Mathematisch-Physikalischen Klasse der Königlichen Bayerischen Akademie der Wissenschaften, Supplement-Band, 1 (6), 1–111.

    Thompson. J., Gibson. T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The CLUSTALX Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tool. Nucleic Acids Research, 24, 4876–4882.

    https://doi.org/10.1093/nar/25.24.4876

    Thornely, L.R. (1900) The hydroid zoophytes collected by Dr Willey in the southern seas. In: Willey, A. (Ed.), Zoological Results based on material from New Britain, New Guinea, Loyalty Islands and elsewhere. Part IV. Cambridge University Press, Cambridge, pp. 451–457.

    Verrill, A.E. (1865) Classification of polyps: (extract condensed from a synopsis of the Polypi of the North Pacific Exploring Expedition, under Captains Ringgold and Rodgers, U.S.N.). Proceedings of the Essex Institute, 4, 145–152.

    https://doi.org/10.1080/00222936508679407

    Vervoort, W. (1968) Report on a Collection of Hydroida from the Caribbean Region: Including an Annotated Checklist of Caribbean Hydroids. Zoologische Verhandelingen, 92 (1), 1–124.

    Wedler, E. (1973) Die Hydroiden der Ciénaga Grande de Santa Marta (Kolumbien) und einiges zu ihrer Ökologie. Mitteilungen aus dem Instituto Colombo-Alemán de Investigaciones Científicas Punta de Betín, 7, 31–39.

    https://doi.org/10.25268/bimc.invemar.1973.7.0.544

    Wedler, E. (1975) Ökologische Untersuchungen an Hydroiden des Felslitorals von Santa Marta (Kolumbien). Helgoländer wissenschaftliche Meeresuntersuchungen, 27 (3), 324–363.

    https://doi.org/10.1007/BF01611700

    Weiss, C.M. (1948) The seasonal occurrence of sedentary marine organisms in Biscayne Bay, Florida. Ecology, 29, 153–172. https://doi.org/10.2307/1932811

    Wenner, E.L., Hinde, P., Knott, D.M. & Van Dolah, R.F. (1984) A temporal and spatial study of invertebrate communities associated with hard-bottom habitats in the South Atlantic Bight. United States Department of Commerce, National Oceanic and Atmospheric Administration, National Marine Fisheries Service, NOAA Technical Report NMFS, 18, 1–104.

    Wyttenbach, C.R. (1969) Genetic variations in the mode of stolon growth in the hydroid, Campanularia flexuosa. The Biological Bulletin, 137 (3), 547–556.

    https://doi.org/10.2307/1540175