Abstract
The frog family Ranixalidae is endemic to the Western Ghats of Peninsular India and contains two genera, Indirana and Walkerana. The three known species of Walkerana are restricted to different hill ranges south of the Palghat Gap, an ancient valley in the Western Ghats. In this study, we report the discovery of a deeply divergent lineage of Walkerana from the high elevations of the Elivalmalai hill range. This finding extends the geographic range of the Walkerana clade to the north of the Palghat Gap. The new species Walkerana muduga sp. nov. is genetically and morphologically divergent, and geographically isolated from its sister lineages. We also recovered a potential new lineage in the adjoining hill ranges suggesting the presence of additional new species in this genus north of the Palghat Gap.
References
Akaike, H. (1974) A New Look at the Statistical Model Identification. In: Parzen E., Tanabe K., Kitagawa G. (Eds.), Selected Papers of Hirotugu Akaike. Springer Series in Statistics (Perspectives in Statistics). Springer, New York, pp. 716–723.
https://doi.org/10.1007/978-1-4612-1694-0_16
Biju, S.D., Bocxlaer, I.V., Mahony, S., Dinesh, K.P., Radhakrishnan, C., Zachariah, A., Giri, V. & Bossuyt, F. (2011) A taxonomic review of the Night Frog genus Nyctibatrachus Boulenger, 1882 in the Western Ghats, India (Anura: Nyctibatrachidae) with description of ten new species. Zootaxa, 3029 (1), 1–96.
https://doi.org/10.11646/zootaxa.3029.1.1
Bocxlaer, I.V., Biju, S.D., Willaert, B., Giri, V.B., Shouche, Y.S. & Bossuyt, F. (2012) Mountain-associated clade endemism in an ancient frog family (Nyctibatrachidae) on the Indian subcontinent. Molecular Phylogenetics and Evolution, 62, 839–847.
https://doi.org/10.1016/j.ympev.2011.11.027
Bocxlaer, I.V., Roelants, K., Biju, S.D., Nagaraju, J. & Bossuyt, F. (2006) Late Cretaceous vicariance in Gondwanan amphibians. PLoS ONE, 1(1), e74.
https://doi.org/10.1371/journal.pone.0000074
Boulenger, G.A. (1882) Catalogue of the Batrachia Salientia S. Ecaudata in the Collection of the British Museum. 2nd Edition. Taylor and Francis, London, 495 pp.
Dahanukar, N., Modak, N., Krutha, K., Nameer, P.O., Padhye, A.D. & Molur, S. (2016) Leaping frogs (Anura: Ranixalidae) of the Western Ghats of India: An integrated taxonomic review. Journal of Threatened Taxa, 8, 9221–9288.
https://doi.org/10.11609/jott.2532.8.10.9221-9288
Darriba, D., Taboada, G., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9, 772.
https://doi.org/10.1038/nmeth.2109
Dinesh, K.P., Radhakrishnan, C., Channakeshavamurthy, B.H., Deepak, P. & Kulkarni, N.U. (2019) A Checklist of Amphibians of India, updated till January 2019. Available from: http://zsi.gov.in/WriteReadData/userfiles/file/Checklist/Amphibia_Checklist_2019.pdf (accessed 11 January 2019)
Dinesh, K.P., Vijayakumar, S.P., Channakeshavamurthy, B.H., Torsekar, V.R., Kulkarni, N.U. & Shanker, K. (2015) Systematic status of Fejervarya (Amphibia, Anura, Dicroglossidae) from South and SE Asia with the description of a new species from the Western Ghats of Peninsular India. Zootaxa, 3999 (1), 79–94.
http://dx.doi.org/10.11646/zootaxa.3999.1.5
Dubois, A. (1992) Notes sur la classification des Ranidae (Amphibiens anoures). Bulletin Mensuel de la Société Linnéenne de Lyon, 6, 305–352.
https://doi.org/10.3406/linly.1992.11011
Frost, D.R. (2019) Amphibian Species of the World: an Online Reference. Version 6.0. American Museum of Natural History, New York. Available from: http://research.amnh.org/herpetology/amphibia/index.html (accessed 12 April 2019)
Garg, S. & Biju, S.D. (2016) Molecular and morphological study of leaping frogs (Anura, Ranixalidae) with description of two new species. PLoS ONE, 11 (11), e0166326.
https://doi.org/10.1371/journal.pone.0166326
Garg, S. & Biju, S.D. (2019) New microhylid frog genus from Peninsular India with Southeast Asian affinity suggests multiple Cenozoic biotic exchanges between India and Eurasia. Scientific Reports, 9, 1906.
https://doi.org/10.1038/s41598-018-38133-x
Günther, A.C.L.G. (1875) Third report on collections of Indian reptiles obtained by the British Museum. Proceedings of the Zoological Society of London, 1875, 567–577.
Inger, R.F., Shaffer, H.B., Koshy, M. & Bakde, R. (1984) A report on a collection of amphibians and reptiles from Ponmudi, Kerala, South India. Journal of Bombay Natural History Society, 81, 406–427.
Kanagavel, A., Parvathy, S., Chundakatil, A.P., Dahanukar, N. & Tapley, B. (2018) Distribution and habitat associations of the Critically Endangered frog Walkerana phrynoderma (Anura: Ranixalidae), with an assessment of potential threats, abundance, and morphology. Phyllomedusa, 17, 21–37.
https://doi.org/10.11606/issn.2316-9079.v17i1p21-37
Laurent, R.F. (1986) Sous Classe des Lissamphibiens (Lissamphibia). In: Grassé, P. & Delsol, M. (Eds.), Traité de Zoologie. Anatomie, Systematique, Biologie. 14 (Batraciens). Fascicule 1B. Masson Paris, pp. 594–797.
Menon, M.T. (1996) The encyclopedia of Dravidian tribes. Vol. II. Thiruvananthapuram, Kerala. International School of Dravidian Linguistics, 1996, 1–347.
Modak, N., Dahanukar, N., Gosavi, N. & Padhye, A. (2015) Indirana salelkari, a new species of leaping frog (Anura: Ranixalidae) from Western Ghats of Goa, India. Journal of Threatened Taxa, 7, 7493–7509.
https://doi.org/10.11609/JoTT.o4262.7493-509
Nair, A., Gopalan, S.V., George, S., Kumar, K.S., Teacher, A.G.F. & Merilä, J. (2012a) High cryptic diversity of endemic Indirana frogs in the Western Ghats biodiversity hotspot. Animal Conservation, 15, 489–498.
https://doi.org/10.1111/j.1469-1795.2012.00539.x
Nair, A., Gopalan, S.V., George, S., Kumar, K.S., Teacher, A.G.F. & Merilä, J. (2012b) Endemic Indirana frogs of the Western Ghats biodiversity hotspot. Annales Zoologici Fennici, 49, 257–286.
https://doi.org/10.5735/086.049.0501
Padhye, A.D., Modak, N. & Dahanukar, N. (2014) Indirana chiravasi, a new species of leaping frog (Anura: Ranixalidae) from Western Ghats of India. Journal of Threatened Taxa, 6, 6293–6312.
https://doi.org/10.11609/JoTT.o4068.6293-312
Phuge, S., Dinesh, K.P., Andhale, R., Bhakre, K. & Pandit, R. (2019) A new species of Fejervarya Bolkay, 1915 (Anura: Dicroglossidae) from the northern Western Ghats parts of Maharashtra, India. Zootaxa, 4544 (2), 251–268.
https://doi.org/10.11646/zootaxa.4544.2.6
R Core Team. (2017) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. Available from: https://www.R-project.org/ (accessed 12 December 2019)
Rambaut, A, Drummond, A.J., Xie, D., Baele, G. & Suchard, M.A. (2018) Posterior summarization in Bayesian phylogenetics using Tracer 1.7. Systematic Biology, 67, 901–904.
https://doi.org/10.1093/sysbio/syy032
Roelants, K., Jiang, J. & Bossuyt, F. (2004) Endemic ranid (Amphibia: Anura) genera in southern mountain ranges of the Indian subcontinent represent ancient frog lineages: evidence from the molecular data. Molecular Phylogenetics and Evolution, 31, 730–740.
https://doi.org/10.1016/j.ympev.2003.09.011
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542.
https://doi.org/10.1093/sysbio/sys029
Simon, C., Franke, A. & Martin, A. (1991) The Polymerase Chain Reaction: DNA Extraction and Amplification. In: Hewitt, G.M., Johnston, A.W.B. & Young, J.P.W. (Eds.), Molecular Techniques in Taxonomy. NATO ASI Series. Series H. Cell Biology. 57. Springer, Berlin, Heidelberg, pp. 329–355.
https://doi.org/10.1007/978-3-642-83962-7_22
Vijayakumar, S.P., Dinesh, K.P., Prabhu, M.V. & Shanker, K. (2014) Lineage delimitation and description of nine new species of bush frogs (Anura: Raorchestes, Rhacophoridae) from the Western Ghats Escarpment. Zootaxa, 3893 (4), 451–488.
http://dx.doi.org/10.11646/zootaxa.3893.4.1
Vijayakumar, S.P., Menezes, R.C., Jayarajan, A. & Shanker, K. (2016) Glaciations, gradients, and geography: multiple drivers of diversification of bush frogs in the Western Ghats Escarpment. Proceedings of Royal Society B, 283, 1–8.
https://doi.org/10.1098/rspb.2016.1011
Vijayakumar, S.P., Pyron, R.A., Dinesh, K.P., Torsekar, V., Srikanthan, A.V., Swamy, P., Stanley, E.L., Blackburn, D.C. & Shanker, K. (2019) A new ancient lineage of frog (Anura: Nyctibatrachidae: Astrobatrachinae subfam. nov.) endemic to the Western Ghats of Peninsular India. PeerJ, 7, e6457.
https://doi.org/10.7717/peerj.6457