Skip to main content Skip to main navigation menu Skip to site footer
Article
Published: 2020-01-16

A new species of Megophrys (Amphibia: Anura: Megophryidae) from Vietnam

Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi, Vietnam Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi, Vietnam
Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi, Vietnam Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi, Vietnam
Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi, Vietnam Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi, Vietnam
Hanoi National University of Education, 136 Xuan Thuy, Cau Giay, Hanoi, Vietnam
AG Zoologischer Garten Köln, Riehler Strasse 173, D-50735 Cologne, Germany Institute of Zoology, University of Cologne, ZülpicherStraße 47b, D-50674 Cologne, Germany
Megophrys caobangensis sp. nov. molecular phylogeny taxonomy Cao Bang Province Amphibia

Abstract

A new species of Megophrys is described from Cao Bang Province, northeastern Vietnam based on morphological and molecular data. Morphologically, the new species is distinguishable from its congeners by a combination of the following characters: Size small (SVL 34.9–38.9 mm in males); tympanum visible; vomerine teeth absent; tongue not notched posteriorly; male with a single vocal sac; toes with rudimentary webbing; subarticular tubercles and lateral fringes absent on all digits; nuptial pads present on fingers I and II in males, with spicules; dorsal skin with scattered granules and tubercles; flank with tubercles; dorsum with a X-shaped dorsal ridge; dorsolateral folds prominent; a small horn-like tubercle present at the outer edge of the eyelid; dorsal surface yellowish brown with a dark brown triangle between the eyes, and a dark brown marking along the X-shaped ridge on the back. In the phylogenetic analyses, the new species is unambiguously nested within the subgenus Panophrys with interspecific uncorrected genetic p-distances (16S rRNA gene) varying from 4.26% (compared with M. rubrimera) to 10.80% (compared with M. acuta).

References

  1. Boulenger, G.A. (1908) A revision of the oriental pelobatid batrachians (genus Megalophrys). Proceedings of the Zoological Society of London, 1908, 407–430.

    https://doi.org/10.1111/j.1096-3642.1908.tb01852.x

    Bourret, R. (1937) Notes herpétologiques sur l’Indochine française. XIV. Les batraciens de la collection du Laboratoire des Sciences Naturelles de l’Université. Descriptions de quinze especes ou variétés nouvelles. Annexe au Bulletin Général de l’Instruction Publique, Hanoi, 1937, 5–56.

    Bourret, R. (1942) Les Batraciens de l’Indochine. Institut Océanographique de l’Indochine, Hanoi, 567 pp.

    Chan-ard, T., Nabhitabhata, J. & Suwannapakdi, K. (2007) On the definite occurrence of Brachytarsophrys feae (Boulenger, 1887) (Anura: Megophryidae) in Northern Thailand. Thailand Natural History Museum Journal, 2 (1), 27–30.

    Chen, J.M., Zhou, W.W., Poyarkov, N.A.J., Stuart, B.L., Brown, R.M., Lathrop, A., Wang, Y.Y., Yuan, Z.Y., Jiang, K., Hou, M., Chen, H.M., Suwannapoom, C., Nguyen, S.N., Duong, T.V., Papenfuss, T.J., Murphy, R.W., Zhang, Y.P. & Che, J. (2017) A novel multilocus phylogenetic estimation reveals unrecognized diversity in Asian horned toads, genus Megophrys sensu lato (Anura: Megophryidae). Molecular Phylogenetics and Evolution, 106, 28–43.

    https://doi.org/10.1016/j.ympev.2016.09.004

    Fei, L., Hu, S., Ye C. & Huang, Y. (2009) Fauna Sinica. Amphibia. Vol. 2. Anura. Chinese Academy of Science, Science Press, Beijing, 957 pp. [in Chinese]

    Fei, L., Ye, C.Y. & Huang, Y. (1992) Description of two new species of the genus Megophrys, Pelobatidae (Amphibia: Anura) from China. Zoological Research, 13 (1), 5–12.

    Fei, L., Ye, C. & Jiang, J. (2010) Colored Atlas of Chinese Amphibians. Sichuan Publishing House of Science & Technology, Sichuan, 519 pp. [in Chinese]

    Fouquet, A., Gilles, A., Vences, M., Marty, C., Blanc, M. & Gemmell, N.J. (2007) Underestimation of species richness in Neotropical frogs revealed by mtDNA analyses. PLOS ONE, 2 (10), e1109.

    https://doi.org/10.1371/journal.pone.0001109

    Frost, D.R. (2019) Amphibian Species of the World: an on-line reference. Version 6.0. American Museum of Natural History, New York. Available form: http://research.amnh.org/herpetology/amphibia/index.html (accessed 12 October 2019)

    Glaw, F. & Vences, M. (2007) A field guide to the amphibians and reptiles of Madagascar. Third Edition, Frosch Verlag, Cologne, 496 pp.

    Huang, C.M., Lathrop, A. & Murphy, R.W. (1998) Translations of “Two new species of amphibian in Tibet” Huang & Fei 1981 and “Description of two new species of the genus Megophrys” Fei, Ye, & Huang 1995. Smithsonian Herpetological Information Service, 118, 1–20.

    https://doi.org/10.5479/si.23317515.118.3

    Huelsenbeck, J.P. & Hillis, D.M. (1993) Success of phylogenetic methods in the four-taxon case. Systematic Biology, 42 (3), 247−264.

    https://doi.org/10.1093/sysbio/42.3.247

    Inger, R.F. & Iskandar, D.T. (2005) A collection of amphibians from West Sumatra, with description of a new species of Megophrys. Raffles Bulletin of Zoology, 52 (1), 133–142.

    Inger, R.F. & Romer, J.D. (1961) A new pelobatid frog of the genus Megophrys from Hong Kong. Fieldiana, 39 (46), 533–538.

    https://doi.org/10.5962/bhl.title.3373

    Jiang, J.P., Ye, C.Y. & Fei, L. (2008) A new horn toad Megophrys sangzhiensis from Hunan, China (Amphibia, Anura). Zoological Research, 29, 219–222. [in Chinese]

    https://doi.org/10.3724/SP.J.1141.2008.00219

    Jobb, G. (2011) Treefinder Version of March 2011. Software Distributed by the Author. Available form: http://www.treefinder.de/ (accessed 12 September 2019)

    Katoh, K. & Standley, D.M. (2013) MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. Molecular Biology and Evolution, 30, 772–780.

    https://doi.org/10.1093/molbev/mst010

    Kou, Z. (1985) A new species of Ophryophryne from Yunnan. Acta Herpetological Sinica, 4, 41–43. [in Chinese]

    Kuraishi, N., Matsui, M., Hamidy, A., Belabut, D.M., Ahmad, N., Panha, S., Sudin, A., Yong, H.S., Jiang, J.P., Ota, H., Thong, H.T. & Nishikawa, K. (2013) Phylogenetic and taxonomic relationship of the Polypedates leucomystax complex (Amphibia). Zoologica Scripta, 42, 54–70.

    https://doi.org/10.1111/j.1463-6409.2012.00562.x

    Le, D.T., Pham, A.V., Nguyen, S.L.H., Ziegler, T. & Nguyen, T.Q. (2015) First records of Megophrys daweimontis Rao and Yang, 1997 and Amolops vitreus (Bain, Stuart and Orlov, 2006) (Anura: Megophryidae, Ranidae) from Vietnam. Asian Herpetological Research, 6, 66–72.

    https://doi.org/10.16373/j.cnki.ahr.140045

    Leaché, A.D. & Reeder, T.W. (2002) Molecular systematics of the eastern fence lizard (Sceloporus undulatus): a comparison of parsimony, likelihood, and Bayesian approaches. Systematic Biology, 51, 44–68.

    https://doi.org/10.1080/106351502753475871

    Li, S., Xu, N., Liu, J., Jiang, J., Weim, G. & Wang, B. (2018) A new species of the Asian toad genus Megophrys sensu lato (Amphibia: Anura: Megophryidae) from Guizhou Province, China. Asian Herpetological Research, 9 (4), 224–239.

    https://doi.org/10.16373/j.cnki.ahr.180072

    Li, Y.L., Jin, M.J., Zhao, J., Liu, Z.Y., Wang, Y.Y. & Pang, H. (2014) Description of two new species of the genus Megophrys (Amphibia: Anura: Megophryidae) from Heishiding Nature Reserve, Fengkai, Guangdong, China, based on molecular and morphological data. Zootaxa, 3795 (4), 449–471.

    https://doi.org/10.11646/zootaxa.3795.4.5

    Liao, C., Liang, Q., Wang, Y. & Jiang, J. (2013) Additional notes on Megophrys sangzhiensis Jiang, Ye et Fei, 2008 (Amphibian, Anuara, Megophryidae). Sichuan Journal of Zoology, 32 (2), 217–218. [in Chinese]

    https://doi.org/10.3969/j.isn.1000-7083.2003.02.011

    Liu, C.C. (1950) Amphibians of western China. Fieldiana Zoology, 2, 1–410.

    https://doi.org/10.5962/bhl.part.4737

    Liu, Z., Chen, G., Zhu, T., Zeng, Z., Lyu, Z., Wang, J., Messenger, K., Greenberg, A.J., Guo, Z., Yang, Z., Shi, S. & Wang, Y. (2018) Prevalence of cryptic species in morphologically uniform taxa-Fast speciation and evolutionary radiation in Asian frogs. Molecular Phylogenetics and Evolution, 127, 723–731.

    https://doi.org/10.1016/j.ympev.2018.06.020

    Lv, J.C., Niu, K.F., Li, S.Z., Li C., Wei, G. & Xu, N. (2014) Brachytarsophrys chuannanensis found in Fanjingshan National Natrure Reserve, Guizhou Province. Chinese Journal of Zoology, 49 (3), 432–434. [in Chinese]

    Mahony, S. (2011) Two new species of Megophrys Kuhl & van Hasselt (Amphibia: Megophryidae), from western Thailand and southern Cambodia. Zootaxa, 2734 (1), 23–39.

    https://doi.org/10.11646/zootaxa.2734.1.2

    Mahony, S., Foley, N., Biju, S.D. & Teeling, E. (2017) Evolutionary history of the Asian Horned Frogs (Megophryinae): integrative approaches to timetree dating in the absence of a fossil record. Molecular Biology and Evolution, 34 (3), 744–771.

    https://doi.org/10.1093/molbev/msw267

    Mahony, S., Sengupta, S., Kamei, R.G. & Biju, S.D. (2011) A new low altitude species of Megophrys Kuhl and van Hasselt (Amphibia: Megophryidae), from Assam, Northeast India. Zootaxa, 3059 (1), 36–46.

    https://doi.org/10.11646/zootaxa.3059.1.2

    Mahony, S., Teeling, E.C.& Biju, S.D. (2013) Three new species of horned frogs, Megophrys (Amphibia: Megophryidae), from northeast India, with a resolution to the identity of Megophrys boettgeri populations reported from the region. Zootaxa, 3722 (2), 143–169.

    https://doi.org/10.11646/zootaxa.3931.2.3

    Malkmus, R. & Matsui, M. (1997) Megophrys kobayashii, ein neuer pelobatider Frosch vom Mount Kinabalu. Sauria, 19, 31–37.

    Manthey, U. & Grossmann, W. (1997) Amphibien and Reptilien Südostasiens. Natur und Tier, Münster, 512 pp.

    Messenger, K.R., Dahn, H.A., Liang, Y., Xie, P., Wang, Y. & Lu, C. (2019). A new species of the genus Megophrys Gunther, 1864 (Amphibia: Anura: Megophryidae) from Mount Wuyi, China. Zootaxa, 4554 (2), 561–583.

    https://doi.org/10.11646/zootaxa.4554.2.9

    Mo, X.Y., Shen, Y.H., Li, H.H. & Wu, X.S. (2010) A new species of Megophrys (Amphibia: Anura: Megophryidae) from the northwestern Hunan Province, China. Current Zoology, 56, 432–436.

    https://doi.org/10.1093/czoolo/56.4.432

    Neang, T., Chin, S., Meang, M. & Hun, S. (2013) Confirmation of three species of megophryid frogs (Amphibia: Megophryidae) from the Cardamom Mountains of Southwest Cambodia, with the rediscovery of a long lost species. Cambodian Journal of Natural History, 2013 (2), 66–72.

    Nguyen, S.V., Ho, T.C. & Nguyen, T.Q. (2009) Herpetofauna of Vietnam. Edition Chimaira, Frankfurt am Main, 768 pp.

    Nguyen, T.T., Le, D.T., Nguyen, S.H.L., Matsui, M. & Nguyen, T.Q. (2014) First record of Philautus petilus Stuart and Heatwole, 2004 (Amphibia: Anura: Rhacophoridae) from Vietnam and its phylogenetic position. Current Herpetology, 33, 112–120.

    https://doi.org/10.5358/hsj.33.112

    Nguyen, T.V., Hoang, C.V., Tran, T.T. & Nguyen, T.Q. (2016) Redescription and new record of Xenophrys jingdongensis (Fei & Ye, 1983) (Anura: Megophryidae) from Ha Giang Province, Vietnam. In: Proceeding of the 3nd National Scientific Workshop on Amphibians and Reptililes in Vietnam. Publishing House for Science and Technology, Hanoi, pp. 111–115.

    Ohler, A. (2003) Revision of the genus Ophryophryne Boulenger, 1903 (Megophryidae) with description of two new species. Alytes, 21, 23–44.

    Ohler, A., Swan, S.R. & Daltry, J.C. (2002) A recent survey of the amphibian fauna of the Cardomom Mountains, Southwest Cambodia with descriptions of three new species. Raffles Bulletin of Zoology, 50 (2), 465–481.

    Ohler, A., Wollenberg, K.C., Grosjean, S., Hendrix, R., Vences, M., Ziegler, T. & Dubois, A. (2011) Sorting out Lalos: description of new species and additional taxonomic data on megophryid frogs from northern Indochina (genus Leptolalax, Megophryidae, Anura). Zootaxa, 3147 (1), 1–83.

    https://doi.org/10.11646/zootaxa.3147.1.1

    Orlov, N.L., Poyarkov Jr., N.A. & Nguyen, T.T. (2015) Taxonomic notes on Megophrys frogs (Megophryidae: Anura) of Vietnam, with description of a new species. Russian Journal of Herpetology, 22 (3), 206–218.

    Pope, C.H. (1929) Four new frogs from Fukien Province, China. American Museum Novitates, 352, 1–5.

    Poyarkov, N.J.A., Duong, T.V., Orlov, N.L., Gogoleva, S.S., Vassilieva, A.B., Nguyen, L.T., Nguyen, V.D.H., Nguyen, S.N., Che, J. & Mahony, S. (2017) Molecular, morphological and acoustic assessment of the genus Ophryophryne (Anura, Megophryidae) from Langbian Plateau, southern Vietnam, with description of a new species. ZooKeys, 672, 49–120.

    https://doi.org/10.3897/zookeys.672.10624

    Rambaut, A. & Drummond, A.J. (2009) Tracer. Version 1.5. Computer program. Available from: http://beast.bio.ed.ac.uk (accessed 20 September 2019)

    Rao, D.Q. & Yang, D.T. (1997) The karyotypes of Megophryinae (Pelobatidae) with a discussion on their classification and phylogenetic relationships. Asian Herpetological Research, 7, 93–102.

    https://doi.org/10.5962/bhl.part.18858

    Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19 (12), 1572–1574.

    https://doi.org/10.1093/bioinformatics/btg180

    Shen, Y., Gu, Q. & Liao, C.L. (2013) Additional record of Megophrys caudoprocta (Amphibia, Megophryidae). Chinese Journal of Zoology, 48 (3), 351–356. [in Chinese]

    Simmons, J.E. (2002) Herpetological collecting and collections management. Revised edition. Society for the Study of Amphibians and Reptiles. Herpetological Circulars, 31, 1–153.

    Smith, M.A. (1921) New or little-known reptiles and batrachians from Southern Annam (Indochina). Proceedings of the Zoological Society of London, 1921, 423–440.

    https://doi.org/10.1111/j.1096-3642.1921.tb03271.x

    Stuart, B.L., Chuaynkern, Y., Chan-ard, T., Inger, R.F. (2006) Three new species of frogs and a new tadpole from eastern Thailand. Fieldiana, 111, 1–19.

    https://doi.org/10.3158/0015-0754(2006)187[1:TNSOFA]2.0.CO;2

    Tanabe, A.S. (2011) Kakusan 4 and Aminosan: two programs for comparing nonpartitioned, proportional and separate models for combined molecular phylogenetic analyses of multilocus sequence data. Molecular Ecology Resources, 11, 914– 921.

    https://doi.org/10.1111/j.1755-0998.2011.03021.x

    Tapley, B., Cutajar, T., Mahony, S., Nguyen, C.T., Dau, V.Q., Luong, A.M., Le, D.T., Nguyen, T.T., Nguyen, T.Q., Portway, C., Luong, H.V. & Rowley, J.J.L. (2018) Two new and potentially highly threatened Megophrys Horned frogs (Amphibia: Megophryidae) from Indochina’s highest mountains. Zootaxa, 4508 (3), 301–333.

    https://doi.org/10.11646/zootaxa.4508.3.1

    Tapley, B., Cutajar, T.P., Mahony, S., Nguyen, C.T., Dau, V.Q., Nguyen, T.T., Luong, H.V. & Rowley, J.J.L. (2017) The Vietnamese population of Megophrys kuatunensis (Amphibia: Megophryidae) represents a new species of Asian horned frog from Vietnam and southern China. Zootaxa, 4344 (3), 465–492.

    https://doi.org/10.11646/zootaxa.4344.3.3

    Tavaré, S. (1986) Some probabilistic and statistical problems in the analysis of DNA sequences. In: Miura, R.M. (Ed.), Some Mathematical Questions in Biology—DNA Sequence Analysis. American Mathematical Society, Providence, Rhode Island, pp. 57–86.

    Taylor, E.H. (1962) The amphibian fauna of Thailand. University of Kansas Science Bull, 43, 265–599.

    https://doi.org/10.5962/bhl.part.13347

    Wang, J., Liu, Z.Y., Lyu, Z.T., Zeng, Z.C. & Wang, Y. (2017b) A new species of the genus Xenophrys (Amphibia: Anura: Megophryidae) from an offshore island in Guangdong Province, southeastern China. Zootaxa, 4324 (3), 541–556.

    https://doi.org/10.11646/zootaxa.4324.3.8

    Wang, J., Lyu, Z.T., Liu, Z.Y., Liao, C.K., Zeng, Z.C., Zhao, J., Li, Y.L. & Wang Y.Y. (2019) Description of six new species of the subgenus Panophrys within the genus Megophrys (Anura, Megophryidae) from southeastern China based on molecular and morphological data. ZooKeys, 851, 113–164.

    https://doi.org/10.3897/zookeys.851.29107

    Wang, Y.D., Zhang, T.D., Zhao, J., Sung, Y.H., Yang, J.H., Pang, H. & Zhang, Z. (2012) Description of a new species of the genus Xenophrys Günther, 1864 (Amphibia: Anura: Megophryidae) from Mount Jinggang, China, based on molecular and morphological data. Zootaxa, 3546 (1), 53–67.

    https://doi.org/10.11646/zootaxa.3546.1.4

    Wang, Y.D, Zhao, J., Yang, J., Zhou, Z., Chen, G. & Liu, Y. (2014) Morphology, molecular genetics, and bioacoustics support two new sympatric Xenophrys toads (Amphibia: Anura: Megophryidae) in Southeast China. PLOS ONE, 9 (4), 1–15.

    https://doi.org/10.1371/journal.pone.0093075

    Wang, Y.F., Liu, B.Q., Jiang, K., Jin, W., Xu, J.N. & Wu, C.H. (2017a) A new species of the Horn Toad of the genus Xenophrys from Zhejiang, China (Amphibia: Megophryidae). Chinese Journal of Zoology, 52, 19–29. [in Chinese]

    https://doi.org/10.13859/j.cjz.201701003

    Xu, J., Zou, P.Z., Wen, C.Y. & Chen, J.R. (2005) A new record of Megophrys from Guangdong Province (Amphibian: Megophrydae). Sichuan Journal of Zoology, 24 (1), 55–56. [in Chinese]

    Yang, J.H., Wang, J. & Wang, Y.Y. (2018) A new species of the genus Megophrys (Anura: Megophryidae) from Yunnan Province, China. Zootaxa, 4413 (2), 325–338.

    https://doi.org/10.11646/zootaxa.4413.2.5

    Ye, C.Y., Fei, L. & Xie, L. (2007) A new species of Megophryidae-Megophrys baolongensis from China (Amphibia, Anura). Herpetological Sinica, 11, 38–41. [in Chinese]

    Zhang, Y., Li, G., Xiao, N., Li, J., Pan, T., Wang, H., Zhang, B. & Zhou, J. (2017) A new species of the genus Xenophrys (Amphibia: Anura: Megophryidae) from Libo County, Guizhou, China. Asian Herpetological Research, 8, 75–85.

    https://doi.org/10.16373/j.cnki.ahr.160041

    Zhao, J., Yang, J., Chen, G.L., Chen, C.Q. & Wang, Y.Y. (2014) Description of a new species of the genus Brachytarsophrys Tian and Hu, 1983 (Amphibia: Anura: Megophryidae) from Southern China based on molecular and morphological data. Asian Herpetological Research, 5 (3), 150–160.

    https://doi.org/10.3724/SP.J.1245.2014.00150