Mitogenomes are popular sources of data in evolutionary studies. By development of next generation sequencing the number of total mitogenome in data bases rapidly increased. However, there is still a limited number of total mitogenome known from species of Tettigoniinae. This paper aims to describe the total mitogenome of Psorodonotus venosus (Orthoptera, Tettigoniidae; Tettigoniinae) obtained by NGS reads. The total mitogenome is 15836-15845 bp and consists of 13 protein coding genes (PCG), 22 tRNA genes, two rRNA genes and an AT rich control region as in other metazozans. The mitogenome is AT skewed with 69.5% AT percentage. The genes are ordered as in pancrustacean. Total length of PCGs is 11229 bp, the start codon for all fits ATN pattern and stop codons are incomplete T-- / TA- and rarely complete TAA. Total length of 22 tRNA genes is 1447 bp and their anticodons are identical to other members of Tettigonioidea. The mitogenome contains 12 overlapping regions constituting 41 bp in total. Of these 12 overlapping regions those between trnW-trnC, atp6-atp8, nad4-nad4L, nad6 –cytb and atp6-cox3 gene pairs seem to be conserved. The total length of seven noncoding intergenic spacers is 46 bp. We concluded that P. venosus is one of the species with short mitogenome amongst Tettigonioidea because of limited number and length of noncoding intergenic spacers.
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