Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2019-05-23
Page range: 269–288
Abstract views: 110
PDF downloaded: 4

A new species of dink frog, genus Diasporus (Anura: Eleutherodactylidae), from the Caribbean foothills of the Cordillera de Talamanca, Costa Rica

Departamento de Zoología, Instituto de Biología, UNAM, AP 70-153 Ciudad Universitaria, CP 04510, Ciudad de México, México. Escuela de Biología, Universidad de Costa Rica, San Pedro, 11501-2060 San José, Costa Rica.
Escuela de Biología, Universidad de Costa Rica, San Pedro, 11501-2060 San José, Costa Rica.
La Esperanza, Horquetas, Sarapiquí, Limón, Costa Rica.
Escuela de Biología, Universidad de Costa Rica, San Pedro, 11501-2060 San José, Costa Rica. Veragua Foundation for the Rainforest Research, Limón, Costa Rica.
Departamento de Zoología, Instituto de Biología, UNAM, AP 70-153 Ciudad Universitaria, CP 04510, Ciudad de México, México. Escuela de Biología, Universidad de Costa Rica, San Pedro, 11501-2060 San José, Costa Rica.
Amphibia Brachycephaloidea Isthmian Central America Panama Taxonomy Terrarana

Abstract

A new dink frog (Eleutherodactylidae: Diasporus) is described from the Tropical Wet Forest, in the northeastern foothills of Cordillera de Talamanca in Costa Rica at an elevation of ca. 1000 m. Analysis of DNA sequences of the 16S rRNA (16S) and cytochrome oxidase 1 (COI) mitochondrial genes revealed a distinct lineage within the genus Diasporus. Additional morphological, morphometric, and acoustic analyses support the differences of this lineage, which we recognize as a new species. This new taxon is distinguished from other members of the genus Diasporus inhabiting Isthmian Central America by its unique coloration: dorsum brown to brown-grayish and venter gray-bluish with pale blotches. The new species is distinguished from other members of the genus Diasporus by very significant genetic distances (higher than 5.6 % in 16S and 16.7 % in COI). The new taxon is most closely related to D. vocator from the south pacific of Costa Rica and to an unnamed taxon from western Panama.

 

References

  1. AmphibiaWeb (2018) AmphibiaWeb: Information on Amphibian Biology and Conservation [web application]. Berkeley, California. Available from: http://amphibiaweb.org (accessed 11 July 2018)

    Anger, G.R. & Dean, R. (2010) The birds of Panama: a field guide. Cornell University Press, Nueva York, 456 pp.

    Araya-Salas, M. & Smith-Vidaurre, G. (2016) warbleR: an R package to streamline analysis of animal acoustic signals. Methods in Ecology and Evolution, 8, 184–191.

    https://doi.org/10.1111/2041-210X.12624

    Arias, E., Chaves, G., Crawford, A.J. & Parra-Olea, G. (2016) A new species of the Craugastor podiciferus species group (Anura: Craugastoridae) from the premontane forest of southwestern Costa Rica. Zootaxa, 4132 (3), 347–363.

    https://doi.org/10.11646/zootaxa.4132.3.3

    Arias, E. & Kubicki, B. (2018) A new moss salamander, genus Nototriton (Caudata: Plethodontidae), from the Cordillera de Talamanca, in the Costa Rica-Panama border region. Zootaxa, 4369 (4), 487–500.

    https://doi.org/10.11646/zootaxa.4369.4.2

    Batista, A., Ponce, M. & Hertz, A. (2012) A new species of rainfrog of the genus Diasporus (Anura: Eleutherodactylidae) from Serranía de Tabasará, Panama. Zootaxa, 3410 (1), 51–60.

    https://doi.org/10.11646/zootaxa.3410.1.4

    Batista, A., Köhler, G., Mebert, K., Hertz, A. & Veselý, M. (2016) An integrative approach to reveal speciation and species richness in the genus Diasporus (Amphibia: Anura: Eleutherodactylidae) in eastern Panama. Zoological Journal of the Linnean Society, 178, 267–311.

    https://doi.org/10.1111/zoj.12411

    Bolaños, F. & Wake, D.B. (2009) Two new species of montane web-footed salamanders (Plethodontidae: Bolitoglossa) from the Costa Rica-Panama border region. Zootaxa, 1981, 57–68.

    Boulenger, G.A. (1898) An account of the reptiles and batrachians collected by Mr. W. F. H. Rosenberg in Western Ecuador. Proceedings of the Zoological Society of London, 1898, 107–126.

    https://doi.org/10.1111/j.1096-3642.1898.tb03134.x

    Boza-Oviedo, E., Rovito, S.M., Chaves, G., García-Rodríguez, A., Artavia, L.G., Bolaños, F. & Wake, D.B. (2012) Salamanders from the eastern Cordillera de Talamanca, Costa Rica, with descriptions of five new species (Plethodontidae: Bolitoglossa, Nototriton, and Oedipina) and natural history notes from recent expeditions. Zootaxa, 3309 (1), 36–61.

    https://doi.org/10.11646/zootaxa.3309.1.2

    Castroviejo-Fisher, S., Köhler, J., De La Riva, I. & Padial, J.M. (2017) A new morphologically cryptic species of Phyllomedusa (Anura: Phyllomedusidae) from Amazonian forests of northern Peru revealed by DNA sequences. Zootaxa, 4269 (2), 245–264.

    https://doi.org/10.11646/zootaxa.4269.2.4

    Chaves, G., García-Rodríguez, A., Mora, A. & Leal, A. (2009) A new species of dink frog (Anura: Eleutherodactylidae: Diasporus) from Cordillera de Talamanca, Costa Rica. Zootaxa, 2088, 1–14.

    Cope, E.D. (1875) On the Batrachia and Reptilia of Costa Rica. Journal Academy of Natural Sciences, 2, 93–157.

    Davis, S.D., Heywood, V.H., Herrera-MacBryde, O., Villa-Lobos, J. & Hamilton, A. (1997) Centres of Plant Diversity: a Guide and Strategy for Their Conservation, Vol. 3. The Americas. IUCN Publications Unit, Cambridge, 562 pp.

    Dayrat, B. (2005) Toward integrative taxonomy. Biological Journal of the Linnean Society, 85, 407–415.

    https://doi.org/10.1111/j.1095-8312.2005.00503.x

    de Queiroz, K. (2007) Species concepts and species delimitation. Systematic Biology, 56, 879–886.

    https://doi.org/10.1080/10635150701701083

    Duellman, W.E. & Lehr, E. (2009) Terrestrial-breeding frogs (Strabomantidae) in Peru. Natur- und Tier-Verlag, Münster, 382 pp.

    Fouquet, A., Gilles, A., Vences, M., Marty, C., Blanc, M. & Gemmell, N.J. (2007) Underestimation of species richness in Neotropical frogs revealed by mtDNA analyses. PLoS One, 2, e1109.

    https://doi.org/10.1371/journal.pone.0001109

    Frost, D.R. (2018) Amphibian Species of the World: an Online Reference. Version 6.0. American Museum of Natural History, New York, USA. Available from: http://research.amnh.org/herpetology/amphibia/index.html (accessed 11 July 2018)

    García-Rodríguez, A., Arias, E. & Chaves, G. (2016) Multiple lines of evidence support the species status of the poorly known Diasporus tigrillo and the recently described Diasporus citrinobapheus (Anura: Eleutherodactylidae). Neotropical Biodiversity, 2, 59–68.

    https://doi.org/10.1080/23766808.2016.1168076

    Garrigues, R. & Dean, R. (2014) The birds of Costa Rica: A field Guide. Cornell University Press, Nueva York, 425 pp.

    Hamilton, C.A., Hendrixson, B.E., Brewer, M.S. & Bond, J.E. (2014) An evaluation of sampling effects on multiple DNA barcoding methods leads to an integrative approach for delimiting species: a case study of the North American tarantula genus Aphonopelma (Araneae, Mygalomorphae, Theraphosidae). Molecular Phylogenetics and Evolution, 71, 79–93

    https://doi.org/10.1016/j.ympev.2013.11.007

    Hanken, J., Wake, D.B. & Savage, J.M. (2005) A solution to the large black salamander problem (genus Bolitoglossa) in Costa Rica and Panamá. Copeia, 2005, 227–245.

    https://doi.org/10.1643/CH-04-083R1

    Hedges, S.B., Duellman, W.E. & Heinicke, M.P. (2008) New World direct-developing frogs (Anura: Terrarana): Molecular phylogeny, classification, biogeography, and conservation. Zootaxa, 1737, 1–182.

    Hertz, A., Hauenschild, F., Lotzkat, S. & Köhler, G. (2012) A new golden frog species of the genus Diasporus (Amphibia, Eleutherodactylidae) from the Cordillera Central, western Panama. ZooKeys, 196, 23–46.

    https://doi.org/10.3897/zookeys.196.2774

    Holdridge, L.R. (1967) Life Zone Ecology. Tropical Science Center, San José, Costa Rica, 206 pp.

    IUCN SSC Amphibian Specialist Group (2015) Diasporus vocator. The IUCN Red List of Threatened Species, 2015, e.T57049A3054378.

    https://doi.org/10.2305/IUCN.UK.2015-4.RLTS.T57049A3054378.en

    Kappelle, M., Cleef, A.M. & Chaverri, A. (1992) Phytogeography of Talamanca montane Quercus forests, Costa Rica. Journal of Biogeography, 19, 299–315.

    https://doi.org/10.2307/2845453

    Katoh, K., Rozewicki, J. & Yamada, K.D. (2017) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics, bbx108. [published online]

    https://doi.org/10.1093/bib/bbx108

    Kubicki, B. (2016) A new species of salamander (Caudata: Plethodontidae: Oedipina) from the central Caribbean foothills of Costa Rica. Mesoamerican Herpetology, 3, 819–840.

    Kubicki, B. & Arias, E. (2016) A beautiful new yellow salamander, genus Bolitoglossa (Caudata: Plethodontidae), from the northeastern slopes of the Cordillera de Talamanca, Costa Rica. Zootaxa, 4184 (2), 329–346.

    https://doi.org/10.11646/zootaxa.4184.2.5

    Lanfear, R., Calcott, B., Ho, S.Y. & Guindon, S. (2012) PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution, 29, 1695–1701.

    https://doi.org/10.1093/molbev/mss020

    Ligges, U., Krey, S., Mersmann, O. & Schnackenberg, S. (2016) tuneR: Analysis of music. R package. Available from: https://cran.r-project.org/package=tuneR (accessed 3 May 2017)

    Lleonart, J., Salat, J. & Torres, G.J. (2000) Removing allometric effects of body size in morphological analysis. Journal of Theoretical Biology, 205, 85–93.

    https://doi.org/10.1006/jtbi.2000.2043

    Lynch, J.D. (2001) Three new rainfrogs Eleutherodactylus diastema group Colombia and Panama. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 25, 287–297.

    Meyer, C.P. (2003) Molecular systematics of cowries (Gastropoda: Cypraeidae) and diversification patterns in the tropics. Biological Journal of the Linnean Society, 79, 401–459.

    https://doi.org/10.1046/j.1095-8312.2003.00197.x

    Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop (GCE), 14 November 2010, 1–8.

    https://doi.org/10.1109/GCE.2010.5676129

    Padial, J.M., Miralles, A., De la Riva, I. & Vences, M. (2010) The integrative future of taxonomy. Frontiers in Zoology, 7, 16.

    https://doi.org/10.1186/1742-9994-7-16

    Padial, J.M., Grant, T. & Frost, D.R. (2014) Molecular systematics of terraranas (Anura: Brachycephaloidea) with an assessment of the effects of alignment and optimality criteria. Zootaxa, 3825 (1), 1–132.

    https://doi.org/10.11646/zootaxa.3825.1.1

    Palumbi, S., Martin, A., Romano, S., McMillan, W.O., Stice, L. & Grabowski, G. (1991) The simple fool’s guide to PCR. Version 2.0. Special Publication, Department of Zoology and Kewalo Marine Laboratory, University of Hawaii, Honolulu, Hawaii, 46 pp.

    Paz, A., Ibáñez, R., Lips, K.R. & Crawford, A.J. (2015) Testing the role of ecology and life history in structuring genetic variation across a landscape: a trait‐based phylogeographic approach. Molecular Ecology, 24, 3723–3737.

    https://doi.org/10.1111/mec.13275

    Pérez-Ponce de León, G & Nadler, S.A. (2010) What we don’t recognize can hurt us: A plea for awareness about cryptic species. Journal of Parasitology, 96, 453–464.

    https://doi.org/10.1645/GE-2260.1

    R Core Team (2017) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. Available from: https://www.R-project.org/ (accessed 3 May 2017)

    Rambaut, A., Suchard, M.A., Xie, W. & Drummond, A.J. (2014) Tracer. Version 1.6.1. Available from: http://beast.bio.ed.ac.uk/Tracer (accessed 3 July 2017)

    Renjifo, L.M., Servat, G.P., Goerck, J.M., Loiselle, B.A. & Blake, J.G. (1997) Patterns of species composition and endemism in the northern Neotropics: a case for conservation of montane avifaunas. Ornithological Monographs, 48, 577–594.

    https://doi.org/10.2307/40157554

    Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542.

    https://doi.org/10.1093/sysbio/sys029

    Savage, J.M. (1997) A new species of rainfrog of the Eleutherodactylus diastema group from the Alta Talamanca Region of Costa Rica. Amphibia-Reptilia, 18, 241–247.

    https://doi.org/10.1163/156853897X00125

    Savage, J.M. (2002) The Amphibians and Reptiles of Costa Rica: A Herpetofauna between two Continents, between two Seas. The University of Chicago Press, Illinois, 934 pp.

    Savage, J.M. & Bolaños, F. (2009) An enigmatic frog of the genus Atelopus (Family Bufonidae) from Parque Nacional Chirripó, Cordillera de Talamanca, Costa Rica. Revista de Biología Tropical, 57, 381–386.

    https://doi.org/10.15517/rbt.v57i1-2.11342

    Simpson, G.G. (1951) The species concept. Evolution, 5, 285–298.

    https://doi.org/10.1111/j.1558-5646.1951.tb02788.x

    Smith, M.A., Poyarkov Jr., N.A. & Hebert, P.D.N. (2008) CO1 DNA barcoding amphibians: Take the chance, meet the challenge. Molecular Ecology Resources, 8, 235–246.

    https://doi.org/10.1111/j.1471-8286.2007.01964.x

    Sosa, A. & Guerrel, J. (2013) Riqueza, diversidad y abundancia de anfibios en el bosque nuboso de Cerro Azul, sector Alto Chagres, Parque Nacional Chagres, Panamá. Tecnociencia, 15, 57–75.

    Sueur, J., Aubin, T. & Simonis, C. (2008) Seewave, a free modular tool for sound analysis and synthesis. Bioacoustics, 18, 213–226.

    https://doi.org/10.1080/09524622.2008.9753600

    Sukumaran, J. & Holder, M.T. (2010a) DendroPy: a Python library for phylogenetic computing. Bioinformatics, 26, 1569–1571.

    https://doi.org/10.1093/bioinformatics/btq228

    Sukumaran, J. & Holder, M.T. (2010b) SumTrees: Phylogenetic Tree Summarization. Version 4.4.0. Available from: https://github.com/jeetsukumaran/DendroPy (accessed 6 May 2019)

    Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.

    https://doi.org/10.1093/molbev/mst197

    Taylor, E.H. (1955) Additions to the known herpetological fauna of Costa Rica with comments on other species. No. II. University of Kansas Science Bulletin, 37, 499–575.

    Vences, M., Thomas, M., Bonett, R.M. & Vieites, D.R. (2005) Deciphering amphibian diversity through DNA barcoding: Chances and challenges. Philosophical Transactions of the Royal Society B: Biological Sciences, 360, 1859–1868.

    https://doi.org/10.1098/rstb.2005.1717

    Wake, D.B., Savage, J.M. & Hanken, J. (2007) Montane salamanders from the Costa Rica-Panama border region, with descriptions of two new species of Bolitoglossa. Copeia, 2007, 556–565.

    https://www.jstor.org/stable/25140665

    Wiley, E.O. (1978) The evolutionary species concept reconsidered. Systematic Zoology, 27, 17–26.

    https://doi.org/10.2307/2412809

    Yu, G., Rao, D., Matsui, M. & Yang, J. (2017) Coalescent-based delimitation outperforms distance-based methods for delineating less divergent species: the case of Kurixalus odontotarsus species group. Scientific reports, 7, 16124.

    https://doi.org/10.1038/s41598-017-16309-1

    Zwickl, D.J. (2006) Genetic algorithm approaches for the phylogenetic analysis of large biological sequence datasets under the maximum likelihood criterion. Unpublished Ph.D. Dissertation. The University of Texas, Austin, pp.