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Article
Published: 2019-04-05

A new species of Amazophrynella (Anura: Bufonidae) with two distinct advertisement calls

Instituto de Ciências Biológicas, Universidade Federal do Amazonas, Av. Rodrigo Octávio 6200, 69077-000, Manaus, Amazonas, Brazil Programa de Pós-Graduação em Ecologia, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo 2936, 69011-970, Manaus, Amazonas, Brazil.
Laboratório de Evolução e Genética Animal, Departamento de Genética, Universidade Federal do Amazonas, Av. Gen. Rodrigo Octávio Jordão Ramos, 3000, CEP 69077-000, Manaus, Amazonas, Brazil.
Programa de Pós-Graduação em Ecologia, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo 2936, 69011-970, Manaus, Amazonas, Brazil.
Instituto de Ciências Biológicas, Universidade Federal do Amazonas, Av. Rodrigo Octávio 6200, 69077-000, Manaus, Amazonas, Brazil Laboratório de Evolução e Genética Animal, Departamento de Genética, Universidade Federal do Amazonas, Av. Gen. Rodrigo Octávio Jordão Ramos, 3000, CEP 69077-000, Manaus, Amazonas, Brazil.
Programa de Pós-Graduação em Ecologia, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo 2936, 69011-970, Manaus, Amazonas, Brazil.
Amphibia Amazonia bioacoustics mtDNA Pará Santarém

Abstract

Here we describe a new species of the toad genus Amazophrynella (Bufonidae) from the south margin of the Amazon River in Santarém, state of Pará, Brazilian Amazonia. The new species can be diagnosed by (1) medium body size for the genus: adult females 19.5–20.4 mm SVL, adult males 13.0–14.5 mm SVL; (2) snout acuminate in lateral view; (3) Finger I larger and robust; (4) Finger I nearly equal in size than Finger II; (5) palmar tubercle rounded, covering ¼ of hand; (6) pigmented callus in Finger I in males; in life: (7) brown stain on throat and chest; (8) black dots on venter. Males emit two distinct advertisement calls: the call type 1 consists of a relatively long note (mean 0.248 s) with a mean dominant frequency of 3526 Hz. The call type 2 is arranged in bouts of 6 to 22 notes with mean dominant frequency of 3450 Hz and much shorter note durations (mean 0.034 s). In this study we also redescribe the advertisement call of A. minuta, which also emits a second call type.

 

References

  1. Ávila, R.W., Carvalho, V.T.D., Gordo, M., Kawashita-Ribeiro, R.A. & Morais, D.H. (2012) A new species of Amazophrynella (Anura: Bufonidae) from southern Amazonia. Zootaxa, 3484, 65–74.

    Bioacoustics Research Program (2015) Raven Pro: Interactive Sound Analysis Software. Version 1.5. The Cornell Lab of Ornithology, Ithaca. Available from: http://www.birds.cornell.edu/raven (accessed 18 March 2019)

    Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9, 772.

    https://doi.org/10.1038/nmeth.2109

    Duellman, W.E. (1978) The biology of an equatorial herpetofauna in Amazonian Ecuador. Miscellaneous Publications, University of Kansas Museum of Natural History, 65, 1–352.

    Ferrão, M., Colatreli, O., Fraga, R., Kaefer, I.L., Moravec, J. & Lima, A.P. (2016) High species richness of Scinax (Anura, Hylidae) in a threatened Amazonian landscape revealed by integrative approach. PLoS ONE, 11, e0165679.
    https://doi.org/10.1371/journal.pone.0165679.

    Fouquet, A., Gilles, A., Vences, M., Marty, C., Blanc, M. & Gemmell, N.J. (2007) Underestimation of species richness in Neotropical frogs revealed by mtDNA analyses. PLoS ONE, 2, e1109.

    https://doi.org/10.1371/journal.pone.0001109

    Fouquet, A., Recoder, R., Teixeira Jr, M., Cassimiro, J., Amaro, R.C., Camacho, A., Damasceno, R., Carnaval, A.C., Moritz, C. & Rodrigues, M.T. (2012a) Amazonella Fouquet et al., 2012 (Anura: Bufonidae) junior homonym of Amazonella Lundblad, 1931 (Acari: Unionicolidae): proposed replacement by Amazophrynella nom. nov. Zootaxa, 3244, 68.

    Fouquet, A., Recoder, R., Teixeira, M., Cassimiro, J., Amaro, R.C., Camacho, A., Damasceno, R., Carnaval, A.C., Moritz, C. & Rodrigues, M.T. (2012b) Molecular phylogeny and morphometric analyses reveal deep divergence between Amazonia and Atlantic forest species of Dendrophryniscus. Molecular Phylogenetics and Evolution, 62, 826–838.

    Fouquet, A., Vacher, J-P., Kadosoe, V., Jairam, R. & Ouboter, P. (2015) Checklist of the amphibians of the Sipaliwini area, Suriname. Herpetology Notes, 8, 63–68.

    Frost, D.R. (2019) Amphibian Species of the World: an Online Reference. Available from: http://research.amnh.org/herpetology/amphibia/index.html (accessed 1 March 2019)

    Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.

    Kimura, M. (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120.

    https://doi.org/10.1007/BF01731581

    Köhler, J., Jansen, M., Rodriguez, A., Kok, P.J.R., Toledo, L.F., Emmrich, M., Glaw, F., Haddad, C.F.B., Rödel, M.O. & Vences, M. (2017) The use of bioacoustics in anuran taxonomy: theory, terminology. Zootaxa, 4251 (1), 1–124.
    http://dx.doi.org/10.11646/zootaxa.4251.1

    Kok, P.J. & Kalamandeen, M. (2008) Introduction to the taxonomy of the amphibians of Kaieteur National Park, Guyana. Abc Taxa, Brussels, ix + 278 pp.

    Lescure, J. & Marty, C. (2000) Atlas des Amphibiens de Guyane. Patrimoines Naturels, 45, 1–388.

    Oliveira, R. & Pereira, J.L.G. (2011) A paisagem da fazenda Taperinha. In: Papavero, N. & Overal, W.L. (Org.), Taperinha: histórico das pesquisas de história natural realizadas em uma fazenda da região de Santarém, no Pará, nos séculos XIX e XX. Museu Paraense Emílio Goeldi, Belém, pp. 401–411.

    Ouboter, P.E. & Jairam, R. (2012) Amphibians of Suriname. Brill, Leiden, 388 pp.

    Padial, J.M. & De La Riva, I. (2009) Integrative taxonomy reveals cryptic Amazonian species of Pristimantis (Anura: Strabomantidae). Zoological Journal of the Linnean Society, 155, 97–122.

    https://doi.org/10.1111/j.1096-3642.2008.00424.x

    Padial, J.M., Miralles, A., De la Riva, I. & Vences, M. (2010) The integrative future of taxonomy. Frontiers in Zoology, 7, 1–14.

    https://doi.org/10.1186/1742-9994-7-16

    Palumbi, S.R. (1996) Nucleic acids II: the polymerase chain reaction. In: Hillis, D.M., Moritz, C. & Mable, K.B. (Eds.), Molecular Systematics. Sinauer & Associates Inc., Sunderland, Massachusetts, pp. 205–247.

    Papavero, N. & Overal, W.L. (2011) Taperinha: histórico das pesquisas de história natural realizadas em uma fazenda da região de Santarém, no Pará, nos séculos XIX e XX. Museu Paraense Emílio Goeldi, Belém, 460 pp.

    Posada, D. (2006) ModelTest Server: a web-based tool for the statistical selection of models of nucleotide substitution online. Nucleic Acids Research, 1, 34.

    https://doi.org/10.1093/nar/gkl042

    Rambaut, A., Drummond, A.J., Xie, D., Baele, G. & Suchard, M.A. (2018) Posterior summarisation in Bayesian phylogenetics using Tracer 1.7. Systematic Biology, 67 (5), 901–904.

    https://doi.org/10.1093/sysbio/syy032

    Rojas, R.R., Carvalho, V.T., Gordo, M. & Ávila, R.W. (2014) A new species of Amazophrynella (Anura:Bufonidae) from the Brazilian Guiana Shield. Zootaxa, 3753, 79–95.

    Rojas, R.R., Carvalho, V.T., Ávila, R.W., Farias, I.P., Gordo, M. & Hrbek, T. (2015) Two new species of Amazophrynella (Amphibia: Anura: Bufonidae) from Loreto, Peru. Zootaxa, 3946 (1), 79–103.
    https://doi.org/10.11646/zootaxa.3946.1.3

    Rojas, R.R., Chaparro, J.C., Carvalho, V.T., Ávila, R.W., Farias, I.P., Hrbek, T. & Gordo, M. (2016) Uncovering the diversity inside the Amazophrynella minuta complex: integrative taxonomy reveals a new species of Amazophrynella (Anura: Bufonidae) from southern Peru. ZooKeys, 71, 43–71.

    Rojas, R.R., Fouquet, A., Ron, S.R., Hernández-Ruz, E.J., Melo-Sampaio, P.R., Chaparro, J.C., Vogt, R.C., Carvalho, V.T., Pinheiro, L.C., Ávila, R.W., Farias, I.P., Gordo, M. & Hrbek, T. (2018a) A Pan-Amazonian species delimitation: high species diversity within the genus Amazophrynella (Anura: Bufonidae). PeerJ, 6, e4941.

    https://doi.org/10.7717/peerj.4941

    Rojas, R.R., Carvalho, V.T., Ávila, R.W., Kawashita-Ribeiro, R.A., Hrbek, T. & Gordo, M. (2018b) Description of the advertisement calls of four species of Amazophrynella (Anura: Bufonidae). Zootaxa, 4459 (1), 193–196.

    https://doi.org/10.11646/zootaxa.4459.1.11

    Rojas, R.R., Fouquet, A., Carvalho, V.T., Ron, S., Chaparro, J.C., Vogt, R.C., Ávila, R.W., Farias, I.P., Gordo, M. & Hrbek, T. (2018c) Redescription of the Amazonian tiny tree toad Amazophrynella minuta (Melin, 1941) (Anura: Bufonidae) from its type locality. Zootaxa, 4482 (3), 511–526.

    https://doi.org/10.11646/zootaxa.4482.3.4

    Ronquist, F., Teslenko, M., Van der Mark, P., Ayres, D., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2011) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542.

    https://doi.org/10.1093/sysbio/sys029

    Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Springs Harbor, pp. 1626.

    Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.

    https://doi.org/10.1093/molbev/mst197

    Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1996) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680.

    https://doi.org/10.1093/nar/22.22.4673

    Vences, M., Meike, T., Van der Meijden, A., Chiari, Y. & Vieites, D. (2005) Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians. Frontiers in Zoology, 2, 7–12.

    https://doi.org/10.1186/1742-9994-2-5

    Wells, K.D. (2007) The Ecology and Behavior of Amphibians. The University of Chicago Press, Chicago, 1148 pp.

    https://doi.org/10.7208/chicago/9780226893334.001.0001