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Type: Article
Published: 2019-03-28
Page range: 580–588
Abstract views: 103
PDF downloaded: 2

Nidirana chapaensis (Bourret, 1937), one additional anuran species for the amphibian fauna of China

Key Laboratory for Conserving Wildlife with Small Populations in Yunnan, Southwest Forestry University, Kunming 650224, Yunnan, China
Key Laboratory for Conserving Wildlife with Small Populations in Yunnan, Southwest Forestry University, Kunming 650224, Yunnan, China
Sam Noble Oklahoma Museum of Natural History & Department of Biology, University of Oklahoma, Norman, Oklahoma, USA
College of Life Science and Technology, Honghe University, Mengzi 661199, Yunnan, China
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
Key Laboratory for Conserving Wildlife with Small Populations in Yunnan, Southwest Forestry University, Kunming 650224, Yunnan, China
Imaging Biodiversity Expedition Institute, Beijing100107, Beijing, China
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
Amphibia new records frog China-Vietnam border Yunnan Province


The China-Vietnam border region is a well-known biodiversity hotspot, harboring a striking diversity of species and endemism. However, the largest part of this region is so far understudied due to restrictions by political boundaries and difficulties in accessibility. Consequently, many species have only been reported from one side of the political boundary, despite the presence of continuous habitat on both sides. In this study, we present our discovery of the frog Nidirana chapaensis in southern Yunnan Province, China. This species was previously known only from Vietnam. All major morphometric characters of these Chinese specimens were found to match with the Vietnamese specimens. In addition, our mitochondrial phylogeny suggests that the Chinese population is monophyletic with respect to topotypic Nidirana chapaensis, with uncorrected pairwise distances of 0.2% at the COI gene fragment analyzed. Therefore, we report Nidirana chapaensis as a new member of amphibian fauna of China, describe the morphological variation of the Chinese population, and provide additional natural history data of this species based on our observation from China.



  1. AmphibiaChina. (2018) The database of Chinese amphibians. Electronic Database Kunming Institute of Zoology (CAS), Kunming, Yunnan, China. Available from: (accessed 26 February 2019)

    Boettger, O. (1895) Neue Frösche und Schlangen von den Liukiu-Inseln. Zoologischer Anzeiger, 18, 266–270.

    Boulenger, G.A. (1904) Descriptions of new frogs and snakes from Yunnan. Annals and Magazine of Natural History, 13, 130–135.

    Boulenger, G.A. (1909) Descriptions of four new frogs and a new snake discovered by Mr. H. Sauter in Formosa. Annals and Magazine of Natural History, 4, 492–495.

    Bourret, R. (1937) Notes herpétologiques sur l’Indochine française. XIV. Les batraciens de la collection du Laboratoire des Sciences Naturelles de l’Université. Descriptions de quinze especes ou variétés nouvelles. Annexe au Bulletin Général de l'Instruction Publique. Hanoi, 1937, 5–56.

    Chang, M.L.Y. & Hsü, H.F. (1932) Study of some amphibians from Szechuan. Contributions from the Biological Laboratory of the Science Society of China. Zoological Series, 8, 137–181.

    Che, J., Chen, H., Yang, J., Jin, J., Jiang, K., Yuan, Z., Murphy, R.W. & Zhang, Y. (2012) Universal COI primers for DNA barcoding amphibians. Molecular Ecology Research, 12, 247–258.

    Che, J., Pang, J., Zhao, H., Wu, G., Zhao, E. & Zhang, Y. (2007) Phylogeny of Raninae (Anura: Ranidae) inferred from mitochondrial and nuclear sequences. Molecular Phylogenetics and Evolution, 43, 1–13.

    Chou, W.H. (1999) A new frog of the genus Rana (Anura: Ranidae) from China. Herpetologica, 55, 389–400.

    Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) JModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9, 772.

    Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32, 1792–1797.

    Fei, L., Ye, C.Y. & Jiang, J.P. (2007) A new species of Ranidae Hylarana (Nidirana) hainanensis from China (Amphibia: Anura). Herpetologica Sinica, 11, 1–4

    Fei, L., Hu, S., Ye, C. & Huang, Y. (2009) Fauna Sinica. Amphibia. Vol. 3. Anura. Science Press, Beijing, 889 pp. [pp. 959–1847, in Chinese]

    Grosjean, S., Ohler, A., Chuaynkern, Y., Cruaud, C. & Hassanin, A. (2015) Improving biodiversity assessment of anuran amphibians using DNA barcoding of tadpoles. Case studies from Southeast Asia. Comptes Rendus Biologies, 338, 351–361.

    Holt, B.G., Lessard, J.P., Borregaard, M.K., Fritz, S.A., Araujo, M.B., Dimitrov, D., Fabre, P., Graham, C.H., Graves, G.R., Jonsson, K.A., Noguesbravo, D., Wang, Z. H., Whittaker, R. J., Fjeldsa, J. & Rahbek, C. (2013) An up-date of Wallace’s zoogeographic regions of the world. Science, 339, 4–78.

    Iwai, N. & Shodakagaya, E. (2012) Population structure of an endangered frog (Nidirana subaspera) endemic to the Amami Islands: possible impacts of invasive predators on gene flow. Conservation Genetics, 13 (3), 717–725.

    Jiang, Y., Wei, Z., Han, F., Ni, Q., Yao, Y., Xu, H., Li, L., Rao, D.Q. & Zhang, M. (2017) The complete mitogenome sequence of Tylototriton ziegleri (Amphibia: Caudata). Conservation Genetics Resources, 9 (3), 503–506.

    Kakehashi, R., Kurabayashi, A., Oumi, S., Katsuren, S., Hoso, M. & Sumida, M. (2013) Mitochondrial genomes of Japanese Nidirana frogs (Ranidae, Anura): unique gene arrangements and the phylogenetic position of genus Nidirana. Genes & Genetic Systems, 88 (1), 59–67.

    Lanfear, R., Calcott, B., Ho, S.Y. & Guindon, S. (2012) Partitionfinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution, 29, 1695–1701.

    Le, D.T., Pham, A.V., Nguyen, S.H.L., Ziegler, T. & Nguyen, T.Q. (2014) Babina lini (Chou, 1999) and Hylarana menglaensis Fei, Ye et Xie, 2008, Two additional anuran species for the herprtofauna of Vietnam. Russian Journal of Herpetology, 21, 315–321

    Li, Z., Yu, G., Rao, D. & Yang, J. (2012) Phylogeography and demographic history of Nidirana pleuraden (Anura, Ranidae) in Southwestern China. PLoS ONE, 7 (3), e34013.

    Luu, Q.V., Nguyen, T.Q., Pham, C.T., Dang, K.N., Vu, T.N., Miskovic, S., Bonkowski, M. & Ziegler, T. (2013) No end in sight? Further new records of amphibians and reptiles from Phong Nha—Ke Bang National Park, Quang Binh Province, Vietnam. Biodiversity Journal, 4, 285–300.

    Lyu, Z.T., Zeng, Z.C., Wang, J., Lin, C.Y., Liu, Z.Y. & Wang, Y. (2017) Resurrection of genus Nidirana (Anura: Ranidae) and synonymizing N. caldwelli with N. adenopleura, with description of a new species from China. Amphibia-Reptilia, 38, 483–502.

    Murphy, R.W. (2016) Advances in herpetological research emanating from China. Zoological Research, 37 (1), 4–6.

    Nguyen, V.S., Ho, T.C. & Nguyen, Q.T. (2009) Herpetofauna of Vietnam. Edition Chimaira, Frankfurt am Main, 768 pp.

    Rambaut, A., Suchard, M.A., Xie, D. & Drummond, A.J. (2014) Tracer. Version 1.6. Available from: (accessed 30 June 2017)

    Ronquist, F., Teslenko, M., Der Mark, P.V., Ayres, D.L., Darling, A.E., Hohna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61 (3), 539–542.

    Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A laboratory Manual. 2nd Edition. Cold Spring Harbor Laboratory Press, Cold Springs Harbor, NY, 1659 pp.

    Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30, 131–1313.

    Stuart, B.L. (2005) New frog records from Laos. Herpetology Review, 36, 473–479.

    Suwannapoom, C., Yuan, Z.Y., Chomdej, S., Chen, J.M., Hou, M., Zhao, H.P., Wang, L.J., Nguyen, S.N., Nguyen, T.T., Fei, L., Murphy, R.M. & Che, J. (2016) Taxonomic revision of the Chinese Limnonectes (Anura, Dicroglossidae) with the description of a new species from China and Myanmar. Zootaxa, 4093 (2), 181–200.

    Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular evolutionary genetics analysis (MEGA) software version 6.0. Molecular Biology and Evolution, 30, 2725–2729.

    Yu, D., Zhang, J. & Zheng, R. (2012) The complete mitochondrial genome of Nidirana adenopleura (Anura: Ranidae). Mitochondrial DNA, 23 (6), 423–425.

    Zhang, M., Han, F., Ye, J., Ni, Q., Li, Y., Yao, Y. & Xu, H. (2017) The entire mitochondrial genome of Vietnam Warty Newt Paramesotriton deloustali (Salamandridae: Paramesotriton) with a new distribution record from China. Conservation Genetics Resources, 10, 1–4.

    Zhou, Y., Yang, B., Min, M.S., Li, P., Fong, J., Dong, B., Zhou, Z. & Lu, Y. (2015) Molecular and morphological evidence for Rana kunyuensis as a junior synonym of Rana coreana (Anura: Ranidae). Journal of Herpetology, 49 (2), 302–307.

    Ziegler, T., Tran D.T.A., Nguyen, T.Q., Perl, R.G.B., Wirk, L., Kulisch, M., Lehmann, T., Rauhaus, A., Nguyen, T.T., Le, Q.K. & Vu, T.N. (2014) New amphibian and reptile records from Ha Giang Province, northern Vietnam. Herpetology Notes, 7, 185–201.