Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2019-02-25
Page range: 375–384
Abstract views: 82
PDF downloaded: 1

Triplophysa sanduensis, a new loach species of nemacheilid (Teleostei: Cypriniformes) from South China

Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University School of Life Sciences, Chongqing 400715, China.
Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University School of Life Sciences, Chongqing 400715, China.
Pisces taxonomy Nemacheilidae phylogeny COI sequence


A new species of Nemacheilidae, Triplophysa sanduensis sp. nov., is described here based on specimens collected from a small cave in Dengguang Village, Zhonghe Town, Sandu County, Guizhou Province, China. The new species can be distinguished from its congeners by the following combination of characters: branched dorsal-fin rays 8–9; branched pectoral-fin rays 8–9; branched pelvic-fin rays 5; branched anal-fin rays 5; vertebrae 4+37; snout length 45.5–50.1% of head length; postdorsal length 39.0–42.8% of standard length; caudal-peduncle depth/caudal-peduncle length range from 56.4% to 71.9%; the posterior chamber of the gas bladder degenerated; body elongated, covered by scattered scales; eyes present and normal in size, not reduced or absent; and a complete lateral line. A phylogenetic analysis based on the mitochondrial cytochrome oxidase subunit 1 (COI) gene resolved Triplophysa sanduensis as sister to Triplophysa longliensis. Kimura's 2-parameter genetic distance of the COI gene sequences between these two species was 1.9%.



  1. Chen, Y.R., Yang, J.X. & Xu, G.C. (1992) A new blind loach of Triplophysa from Yunnan stone forest with comments on its phylogenetic relationship. Zoological Research, 13 (1), 17–23.

    Chen, Y.R., Yang, J.X., Sket, B. & Aljancic, G. (1998) A new blind cave loach of Paracobitis with comment on its characters evolution. Zoological Research, 19 (1), 59–63.

    Chen, Y.Y., Chen, Y.F. & Liu, H.Z. (1996) Studies on the position of the Qinghai-Tibet plateau region in zoogeographic divisions and its eastern demarcation line. Acta Hydrobiologica Sinica, 20 (2), 97–103.

    Eschmeyer, W.N., Fricke, R. & Laan, R. (2018) Catalog of fishes: genera, species, references. Online version, updated 5 November 2018. Available from: (accessed 13 November 2018)

    He, C.L., Zhang, E. & Song, Z.B. (2012) Triplophysa pseudostenura, a new nemacheiline loach (Cypriniformes: Balitoridae) from the Yalong River of China. Zootaxa, 3586, 272–280.

    Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16 (2), 111–120.

    Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33 (7), 1870–1874.

    Li, J., Lan, J.H., Chen, X.Y. & Du, L.N. (2017) Description of Triplophysa luochengensis sp. nov. (Teleostei: Nemacheilidae) from a karst cave in Guangxi, China. Journal of Fish Biology, 91 (4), 1009–1017.

    Li, J., Li, X.H., Lan, J.H. & Du, L.N. (2017) A new troglobitic loach Triplophysa tianlinensis (Teleostei: Nemacheilidae) from Guangxi, China. Ichthyological Research, 64 (3), 295–300.

    Li, W.X. & Zhu, Z.G. (2000) A new species of Triplophysa from cave Yunnan. Journal of Yunnan University, 22 (5), 396–398.

    Li, W.X. (2004) The three new species of Cobitidae from Yunnan, China. Journal of Jishou University, 25 (3), 93–96.

    Li, W.X., Yang, H.F., Chen, H., Tao, C.P., Qi, S.Q. & Han, F. (2008) A new blind underground species of the genus Triplophysa (Balitoridae) from Yunnan, China. Zoological Research, 29 (6), 674–678.

    Lin, Y., Li, C. & Song, J.K. (2012) A new species of troglobitic loach, Triplophysa jiarongensis, from Guizhou, China. Acta Zootaxonomica Sinica, 37 (3), 640–647.

    Liu, S.Q., Mayden, R.L., Zhang, J.B., Yu, D., Tang, Q.Y., Deng, X. & Liu, H.Z. (2012) Phylogenetic relationships of the Cobitoidea (Teleostei: Cypriniformes) inferred from mitochondrial and nuclear genes with analyses of gene evolution. Gene, 508 (1), 60–72.

    Ren, Q., Yang, J.X. & Chen, X.Y. (2012) A new species of the genus Triplophysa (Cypriniformes: Nemacheilidae), Triplophysa longliensis sp. nov, from Guizhou, China. Zootaxa, 3586, 187–194.

    Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D., Darling, A., Höhna, S., Larget, B., Liu, L. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542.

    Wu, T.J., Wei, M.L., Lan, J.H. & Du, L.N. (2018) Triplophysa anshuiensis, a new species of blind loach from the Xijiang River, China (Teleostei, Nemacheilidae). Zookeys, 744, 67–77.

    Wu, T.J., Yang, J. & Lan, J.H. (2012) A new blind loach, Triplophysa lihuensis (Teleostei: Balitoridae), from Guangxi, China. Zoological Studies, 51 (6), 874–880.

    Wu, W.J., He, A.Y., Yang, J.X. & Du, L.N. (2018) Description of a new species of Triplophysa (Teleostei: Nemacheilidae) from Guizhou Province, China. Journal of Fish Biology, 93 (1), 88–94.

    Yang, J.X. & Chu, X.L. (1990) Differentiation of three loaches of the genus Triplophysa in Nanpanjiang River basin, Yunnan (Pisces: Cobitidae). Acta Zootaxonomica Sinica, 15 (3), 337–383.

    Yang, J.X. (1990) Nemacheiline. In: Chu, X.L. & Chen, Y.R. (Eds.), The Fishes of Yunnan, China, Part II. Science Press, Beijing, pp. 56–58.

    Yang, J.X., Chen, X.Y. & Lan, J.H. (2004) Occurrence of two new plateau-indicator loaches of Nemacheilinae (Balitoridae) in Guangxi with reference to zoogeographical significance. Zoological Research, 25 (2), 111–116.

    Zhang, C.G., Xing, Y.C., Zhao, Y.H., Zhou, W. & Tang, W.Q. (2015) Species Diversity and Distribution of Inland Fishes in China. Science Press, Beijing, 284 pp.

    Zheng, L.P., Du, L.N., Chen, X.Y. & Yang, J.X. (2009) A new species of Genus Triplophysa (Nemacheilinae: Balitoridae), Triplophysa longipectoralis sp. nov, from Guangxi, China. Environmental Biology of Fishes, 85 (3), 221–227.

    Zhu, S.Q. (1989) The Loaches of the Subfamily Nemacheilinae in China (Cypriniformes: Cobitidae). Jiangsu Science and Technology Publishing House, Nanjing, 150 pp.