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Issue: Vol. 4476 No. 1: 13 Sept. 2018
Type: Article
Published: 2018-09-13
DOI: 10.11646/zootaxa.4476.1.12
Page range: 130–140
Abstract views: 87
PDF downloaded: 97

Testing the validity of two putative sympatric species from Sinocyclocheilus (Cypriniformes: Cyprinidae) based on mitochondrial cytochrome b sequences

School of Life Sciences, Yunnan University, Kunming 650091, China
School of Life Sciences, Yunnan University, Kunming 650091, China
School of Life Sciences, Yunnan University, Kunming 650091, China Key Laboratory for Animal Genetic Diversity and Evolution of High Education in Yunnan Province, Yunnan University, Kunming 650091, China
Heilongtan Reservoir of Shilin County, Yunnan Province, Shilin 652200, China
Fisheries Administration of Qiubei County, Yunnan Province, Qiubei 653200, China
School of Life Sciences, Yunnan University, Kunming 650091, China Key Laboratory for Animal Genetic Diversity and Evolution of High Education in Yunnan Province, Yunnan University, Kunming 650091, China
School of Life Sciences, Yunnan University, Kunming 650091, China
Pisces Mitochondrial DNA CYTB molecular identification

Abstract

There are over 60 species within the freshwater fish genus Sinocyclocheilus (Cypriniformes: Cyprinidae) distributed throughout the Yunnan-Guizhou Plateau and its surrounding areas in China. In recent years, the increasing number of new species described has raised some controversy about the validity of several species within this genus, notably the putative sympatric species pair S. qiubeiensis and S. jiuchengensis. To test the validity of S. qiubeiensis and S. jiuchengensis, we analyzed the complete sequences of the mitochondrial cytochrome b (CYTB) gene of 20 identified species and one outgroup species. Phylogenetic relationships were reconstructed using CYTB with maximum likelihood (ML) and Bayesian inference (BI) methods. Our phylogenetic results showed that all individuals of S. qiubeiensis and S. jiuchengensis clustered in one clade with strong support. In addition, the genetic distance between the two species was 0.11%, within the range observed at the intraspecific level. The most recent common ancestor of S. qiubeiensis and S. jiuchengensis dated back to 0.13 million years ago, indicating little time for complete speciation to have occurred. These results clearly support the viewpoint that S. jiuchengensis is a synonym of S. qiubeiensis.

References

  1. Cantatore, P., Roberti, M., Pesole, G., Ludovico, A., Milella, F., Gadaletal, M.N. & Saccone, C. (1994) Evolutionary analysis of cytochrome b sequences in some perciformes: evidence for a slower rate of evolution than in mammals. Journal of Molecular Evolution, 39, 589–597.
    https://doi.org/10.1007/BF00160404

    Chen, R.Y., Yang, J.X. & Lan, J.H. (1997) One new species of blind cavefish from Guangxi with comments on its phylogenetic status (Cypriniformes: Cyprinidae: Barbinae). Zoological Systematics, 22, 219–233.

    Excoffier, L., Laval, G. & Schneider, S. (2005) Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online, 1, 47–50.
    https://doi.org/10.1177/117693430500100003

    Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39, 783–791.
    https://doi.org/10.1111/j.1558-5646.1985.tb00420.x

    Fu, X.Y. & Li, H.W. (1993) Statistical tests of neutrality of mutations. Genetics, 133, 693–709.

    Gan, X., Wu, T.J., Wei, M.L. & Yang, J. (2013) A new blind barbine species, Sinocyclocheilus anshuiensis sp. nov. (Cypriniformes: Cyprinidae) from Guangxi, China. Zoological Research, 34, 459–463.

    Hillis, D.M. & Bull, J.J. (1993) An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology, 42, 182–192.
    https://doi.org/10.1093/sysbio/42.2.182

    Johns, G.C. & Avise, J.C. (1998) A comparative summary of genetic distances in the vertebrates from the mitochondrial cytochrome b gene. Molecular Biology and Evolution, 15, 1481–1490.
    https://doi.org/10.1093/oxfordjournals.molbev.a025875

    Leaché, A.D. & Reeder, T.W. (2002) Molecular systematics of the Eastern Fence Lizard (Sceloporus undulatus): a comparison of parsimony, likelihood, and Bayesian approaches. Systematic Biology, 51, 44–68.
    https://doi.org/10.1080/106351502753475871

    Leigh, J.W. & Bryant, D. (2015) Popart: full-feature software for haplotype network construction. Methods in Ecology and Evolution, 6, 1110–1116.
    https://doi.org/10.1111/2041-210X.12410

    Li, W.H. & Fu, Y.X. (1993) Statistical tests of neutrality of mutations. Genetics, 133, 693–709.

    Li, G.L. (1989) On a new fish genus Sinocyclocheilus from Guangxi China (Cypriniformes: Cyprinidae: Barbinae). Zoological Systematics, 14, 123–126.

    Li, J.J., Fang, X.M., Pan, B.T., Zhao, Z.J. & Song, Y.G. (2001) Late Cenozoic intensive uplift of Qinghai-Xizang Plateau and its impacts on environments in surrounding area. Quarternary Sciences, 21, 381–391.

    Li, J., Li, X.H. & Mayden, R.L. (2014) Sinocyclocheilus brevifinus (Teleostei: Cyprinidae), a new species of cavefish from Guangxi, China. Zootaxa, 3873 (1), 37–48.
    https://doi.org/10.11646/zootaxa.3873.1.3

    Li, W.X. (1985) Description on four new species of Sinocyclocheilus from Yunnan, China (Pisces: Cyprinidae). Zoological Research, 6, 423–429.

    Li, W.X. & Lan, J.H. (1992) A new genus and three new species Cyprinidae from Guangxi, China. Journal of Zhanjiang Fisheries College, 12, 46–51.

    Li, W.X., Liao, Y.P. & Yang, H.F. (2002) Two new species of Sinocyclocheilus from eastern Yunnan, China. Journal of Yunnan Agricultural University, 17, 161–163.

    Li, W.X., Xiao, H., Zan, R.G., Luo, Z.Y., Ban, C.H. & Fen, J.B. (2003) A new species of Sinocyclocheilus from caves in GuangXi. Journal of Guangxi Normal University, 21, 80–81.

    Li, Z.Q., Guo, B.C., Li, J.B., He, S.P. & Chen, Y.Y. (2008) Bayesian mixed models and divergence time estimation of Chinese cavefishes (Cyprinidae: Sinocyclocheilus). Science Bulletin, 53, 2342–2352.
    https://doi.org/10.1007/s11434-008-0297-2

    Liang, X.F., Cao, L. & Zhang, C.G. (2011) Molecular phylogeny of the Sinocyclocheilus (Cypriniformes: Cyprinidae) fishes in northwest part of Guangxi, China. Environmental Biology of Fishes, 92, 371–379.
    https://doi.org/10.1007/s10641-011-9847-6

    Librado, P. & Rozas, J. (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25, 1451–1452.
    https://doi.org/10.1093/bioinformatics/btp187

    Pan, X.F., Li, L., Yang, J.X. & Chen, X.Y. (2013) Sinocyclocheilus xichouensis, a new species of golden-line fish from the Red River drainage in Yunnan, China (Teleostei: Cypriniformes). Zoological Research, 34, 368–373.

    Peng, J.L., Wang, X.Z., Wang, D. & He, S.P. (2009) Application of DNA barcoding based on the mitochondrial CO1 gene sequences in classification of Culter (Pisces: Cyprinidae). Acta Hydrobiologica Sinica, 33, 271–276.
    https://doi.org/10.3724/SP.J.1035.2009.00271

    Posada, D. (2008) jModelTest: phylogenetic model averaging. Molecular Biology and Evolution, 25, 1253–1256.
    https://doi.org/10.1093/molbev/msn083

    Romero, A. (2001) Scientists prefer them blind: the history of hypogean fish research. Environmental Biology of Fishes, 62, 43–71.
    https://doi.org/10.1023/A:1011830329016

    Romero, A. & Paulson, K.M. (2001) It's a wonderful hypogean life: a guide to the troglomorphic fishes of the world. Environmental Biology of Fishes, 62, 13–41.
    https://doi.org/10.1023/A:1011844404235

    Romero, A., Zhao, Y.H. & Chen, X.Y. (2009) The hypogean fishes of China. Environmental Biology of Fishes, 86, 211–278.
    https://doi.org/10.1007/s10641-009-9441-3

    Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.
    https://doi.org/10.1093/bioinformatics/btg180

    Shan, X.H. & Yue, P.Q. (1994) The study on phylogeny of the Sinocyclocheilus fishes (Cypriniformes: Cyprinidae: Barbinae). Zoological Research, 15, 36–44.

    Swofford, D.L. (2003) PAUP*, Phylogenetic analysis using parsimony (* and other methods). Version 4.Ob10. Sinauer Associates, Sunderland, MA. [software]

    Tajima, F. (1989) Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics, 123, 585–595.

    Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.
    https://doi.org/10.1093/molbev/mst197

    Wang, D., Zhao, Y.H., Yang, J.X. & Zhang, C.G. (2014) A new cavefish species from Southwest China, Sinocyclocheilus gracilicaudatus sp. nov. (Teleostei: Cypriniformes: Cyprinidae). Zootaxa, 3768 (5), 583–590.
    https://doi.org/10.11646/zootaxa.3768.5.5

    Wang, D.Z., Chen, Y.Y. & Li, X.Y. (1999) An analysis on the phylogeny of the genus Sinocyclocheilus. Acta Academiae Medicinae Zunyi, 22, 1–6. [in Chinese]

    Wang, X.Z., Li, J.B. & He, S.P. (2007) Molecular evidence for the monophyly of East Asian groups of Cyprinidae (Teleostei: Cypriniformes) derived from the nuclear recombination activating gene 2 sequences. Molecular Phylogenetics and Evolution, 42, 157–170.
    https://doi.org/10.1016/j.ympev.2006.06.014

    Xia, X. & Xie, Z. (2001) DAMBE: software package for data analysis in molecular biology and evolution. Journal of Heredity, 92, 371–373.
    https://doi.org/10.1093/jhered/92.4.371

    Xiao, H., Chen, S.Y., Liu, Z.M., Zhang, R.D., Li, W.X., Zan, R.G. & Zhang, Y.P. (2005) Molecular phylogeny of Sinocyclocheilus (Cypriniformes: Cyprinidae) inferred from mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 36, 67–77.
    https://doi.org/10.1016/j.ympev.2004.12.007

    Yang, Z.H. (2007) PAML 4: phylogenetic analysis by maximum likelihood. Molecular Biology and Evolution, 24, 1586–1591.
    https://doi.org/10.1093/molbev/msm088

    Zardoya, R. & Meyer, A. (1996) Phylogenetic performance of mitochondrial protein-coding genes in resolving relationships among vertebrates. Molecular Biology and Evolution, 13, 933–942.
    https://doi.org/10.1093/oxfordjournals.molbev.a025661

    Zhao, Y.H. & Zhang, C.G. (2006) Past research and future development on endemic Chinese cavefish of the genus Sinocyclocheilus (Cypriniformes: Cyprinidae). Acta Zootaxonomica Sinica, 31, 769–777.

    Zhao, Y.H. & Zhang, C.G. (2009) Endemic Fishes of Sinocyclocheilus (Cypriniformes: Cyprinidae) in China: Species diversity, Cave adaptation, Systematics and Zoogeography. Science Press, Beijing, 271 pp.

    Zheng, H.F., Xiu, L.H. & Yang, J. (2013) A new species of Barbine genus Sinocyclocheilus (Teleostei: Cyprinidae) from Zuojiang River drainage in Guangxi, China. Environmental Biology of Fishes, 96, 747–751.
    https://doi.org/10.1007/s10641-012-0068-4

    Zhu, D.G., Zhu, Y. & Lan, J.H. (2011) Description of a new species of Barbinae, Sinocyclocheilus huangtianensis from China (Teleostei: Cypinidae). Zoological Research, 32, 204–207.