The critically endangered ‘scarce yellow sally stonefly’ Isogenus nubecula (Newman, 1833) (Plecoptera: Perlodidae) was rediscovered in the United Kingdom (UK) in 2017. This rediscovery comes after a 22-year period of absence despite numerous surveys since its last record in 1995. This species is one of the rarest stoneflies in the UK and Europe and its rediscovery is of international significance, being the westernmost point in Europe where the species is found, with the next nearest populations occurring in Austria and western Hungary, Slovakia, and central Sweden. The species is classed as pRDB2 (vulnerable), however is not listed in the British Red Data Book despite only being present (as far as records detail) in one river, the River Dee in North Wales, UK. Only fourteen individuals were caught and the need for conservation of this rare stonefly is therefore of paramount importance. We have made recommendations for the need to increase survey effort using environmental DNA (eDNA) techniques in order to fully understand the species range in this river and those in the surrounding area. The DNA sequence of I. nubecula has been uploaded on GenBank for further genetic studies. Captive rearing could also be explored with possible reintroductions to sites within its former UK range.
Bowker, J. (1993) A survey of the River Dee for the rare stonefly Isogenus nubecula in relation to the relocation of the Five Fords STW outfall. Environment Agency, Welsh Region, 9 pp.
Bowker, J. (1995) Records of Isogenus nubecula Newman (Plecopt., Perlodidae) from the Welsh River Dee. Entomologist’s Monthly Magazine, 131, 69–70.
Claessens, E.E.C.M (1981) The stoneflies (Plecoptera) of the Netherlands. Nieuwsbrief European Invertebrate Survey – Nederland, 10, 73–77.
Davy-Bowker, J. (2003) A survey of the Welsh River Dee for the rare stonefly Isogenus nubecula Newman. Centre for Ecology & Hydrology, Dorset, 19 pp.
Davy-Bowker, J., Neale, M., Hammett, M.J. & Hull, M. (2007) An airlift study of the Welsh River Dee for the rare stonefly Isogenus nubecula Newman. Countryside Council for Wales, Bangor, UK. CCW Contract Science Report, No. 793, 1–10.
Derka, T, Krno, I & Strechayová, S (2002) New locality of Isogenus nubecula and Amphinemura borealis in central Europe (Plecoptera: Perlodidae, Nemouridae). Entomological Problems, 32 (2), 138.
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.
Graf, W. & Kovács, T. (2002) The aquatic invertebrates of the Lafnitz–Rába river system in Austria and Hungary—a natural heritage of the Central European potamocoen. In: Brezeanu, G. & Stiuca, R. (Eds.), 34th Conference, International Association for Danube Research (IAD), 26–30. August 2002, Tulcea RO. Limnological Reports. No. 34. Editura Academiei Romane, Bucharest, pp. 295–303.
Graf, W., Lorenz, A.W., Tierno de Figueroa, J.M., Lücke, S., López-Rodríguez, M.J. & Davies, C (2009) Distribution and ecological preferences of European freshwater organisms. Vol. 2. Plecoptera. Pensoft, Sofia-Moscow, 262 pp. [Schmidt-Kloiber, A. & Hering, D. (Eds.)]
Hammett, M.J. & Wallace, I.D. (2005) A survey for the stonefly Isogenus nubecula (Newman) on the Welsh River Dee and its tributaries (December 2003 to April 2004). Countryside Council for Wales, Bangor, UK. Contract Science Report, No. 717, 1–10.
Hoffsten, P.-O. (2003) Rarity in boreal stream: patterns, causes and consequences. Department of Ecology and Environmental Science. Umeå University, Umeå, 23 pp.
Hynes, H.B.N. (1963) Isogenus nubecula Newman in Britain (Plecoptera: Perlodidae). Proceedings of the Royal Entomological Society of London A, 38, 12–14.
Hynes, H.B.N. (1977) A key to the adults and nymphs of the British stoneflies (Plecoptera) with notes on their ecology and distribution. Freshwater Biological Association, Scientific Publication, No. 17, 1–90.
Juhász, P., Turcsányi, I., Kovács, T., Olajos, P., Turcsányi, B. & Kiss, B. (1998) Vízi makroszkópikus gerinctelen élőlényegyüttesek vizsgálata a Felső-Tiszán. Hidrológiai Közlöny, 5–6, 346–347.
Kovács, T. & Ambrus, A. (2001) Ephemeroptera, Odonata and Plecoptera larvae from the River Tisza (1997–1999). Folia Historico Naturalia Musei Matraensis, 25, 135–143.
Landa, V., Helesic, J., Soldán, T. & Zahrádková, S. (1997) Stoneflies (Plecoptera) of the River Vltava, Czech Republic: a century of extinction. In: Landolt, P. & Sartori, M. (Eds.), Ephemeroptera & Plecoptera: Biology-Ecology-Systematics. Maron, Tinguely & Lachat SA, CH-Fribourg, pp 288–295.
Macadam, C.R. (2015) Review of the stoneflies (Plecoptera) of Great Britain. Natural England Species Status Report, No 20, 1–41.
Mauvisseau, Q., Coignet, A., Delaunay, C., Pinet, F., Bouchon, D. & Souty-Grosset, C. (2017) Environmental DNA as an efficient tool for detecting invasive crayfishes in freshwater ponds. Hydrobiologia, 805 (1), 163–175.
Millband, H. (1998) The status of the rare stonefly Isogenus nubecula in the middle Dee. Environment Agency, Welsh Region, 5 pp. [NEAT/98/TM11]
Millband, H. (1999) The status of the rare stonefly Isogenus nubecula, in the middle Dee. Environment Agency, Welsh Region, 2 pp. [NEAT/99/TM15]
Mills, M.J. & Andrew, D. (1984) Isogenus nubecula Newman (Plecoptera: Perlodidae) in the Welsh River Dee. Entomologist’s Gazette, 35, 45–51.
Tanner, J.M. (1997) A survey of the Welsh River Dee for the rare stonefly Isogenus nubecula. Environment Agency, Welsh Region, 10 pp.
Thomsen, P.F. & Willerslev, E. (2014) Environmental DNA—An emerging tool in conservation for monitoring past and present biodiversity. Biological Conservation, 183, 4–18.
Zwick, P. (1992) Stream habitat fragmentation—a threat to biodiversity. Biodiversity and Conservation, 1 (2), 80–97.