A taxonomic review of the dwarf species of the genus Eumicrotremus (Actinopterygii: Cottoidei: Cyclopteridae), previously recognized as belonging to Lethotremus, established the existence of three species, viz. Eumicrotremus awae (Jordan and Snyder, 1902), Eumicrotremus uenoi sp. nov., and Eumicrotremus jindoensis sp. nov., from the western North Pacific. Eumicrotremus awae, known from the Pacific coast of Honshu Is. (Chiba southward to Mie), is characterized by the absence of spiny tubercles or fleshy papillae on the body, the anterior three mandibular pores each with a barbel-like tube, interorbital and suborbital pores usually absent, the opercular flap rounded, many papillae present on the ventral disk and the caudal-fin relatively short, 21.0–30.5% of SL (standard length). Eumicrotremus uenoi, known from the western coast of Honshu Is. and the southern coast of Korean Peninsula, is characterized by the absence of spiny tubercles or fleshy papillae on the body (or weak if present), the anterior three mandibular pores each with a barbel-like tube, interorbital and suborbital pores usually present, the opercular flap rounded, the ventral disk with many papillae and the caudal-fin relatively short, 19.5–31.4% of SL. Eumicrotremus jindoensis, known from the southwestern coast of Korean Peninsula, and the coasts of Yellow Sea, is characterized by a smooth soft body, triangular opercular flap, absence of papillae on the ventral disk and a long caudal fin, 32.7–42.1% of SL. Sequence variations of the mictochondrial DNA cytochrome c oxidase subunit I (mtDNA COI) and the nuclear myh6 genes supported the validity of the three species.
Abe, T. & Sato, N. (2009) Reproductive biology of the lumpsucker fish Lethotremus awae in Shizugawa Bay, northern Honshu, Japan. Japanese journal of Ichthyology, 56, 159–163.
Aizawa, M. (2003) Fishes. In: Chiba Shiryo-Kenkyu-Zaidan (Ed.), Zoological Catalog of Chiba Prefecture, Japan. Chiba Prefecture, Chiba, pp. 304–335.
Aoyama, J., Watanabe, S., Ishikawa, S., Nishida, M. & Tsukamoto, K. (2000) Are morphological characters distinctive enough to discriminate between two species of freshwater eels, Anguilla celebesensis and A. interioris? Ichthyolgical Research, 47, 157–161.
Böhlke, J. (1953) A catalog of the type specimens of recent fishes in the Natural History Museum of Stanford University. Stanford Ichthyological Bulletin, 5, 1–168.
Cheng, Q.T. & Zhou, C.W. (1997) The fishes of Shandong Province. Shandong Science and Technology Press, 549 pp.
Chu, Y.T. & Jin, X.B. (1963) Cottoidei. In: Chu, Y.T., Tchang, T.L. & Cheng, Q.T. (Eds.), Fishes of the East China Sea. Science Press, Beijing, pp. 451–504.
Ding, G.W. (1987) Cottoidei. In: Liu, C.X. & Qin, K.J. (Eds.), Fauna Liaoningica. Liaoning Science and Technology Press, Shenyang, pp. 400–414.
Felsenstein, J. (1981) Evolutionary trees from DNA sequencies – a maximum-likelihood approach. Journal of Molecular Evolution, 17, 368–376.
Gilbert, C.H. (1896) The ichthyological collections of the steamer Albatross during the years 1890 and 1891. United States Commission of Fish and Fisheries, Report of the Commissioner, 19, 393–476.
Gill, T.N. (1862) Note on some genera of fishes of western North America. Proceedings of the Academy of Natural Sciences of Philadelphia, 14, 329–332.
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.
Honma, Y., Nakamura, Y., Imura, H., Shoji, T. Honma, R. & Inoue, N. (2008) Further additions to “A list of the fishes collected in the Province of Echigo including Sado Island”. Kashiwazaki City Museum, 22, 65–74.
International Code on Zoological Nomenclature (ICZN) (1999) International Code of Zoological Nomenclature. International Trust for Zoological Nomenclature, London, 306 pp.
Jin, X.B. (2006) Fauna Sinica, Ostichthyes, Scorpaeniformes. Science Press, Beijing, 739 pp.
Jordan, D.S. & Snyder, J.O. (1902) A review of the discobolous fishes of Japan. Proceedings of the United States National Museum, 24, 343–351.
Kai, Y., Stevenson, D.E., Ueda, Y., Hamatsu, T. & Nakabo, T. (2015) Molecular insights into geographic and morphological variation within the Eumicrotremus asperrimus species complex (Cottoidei: Cyclopteridae). Ichthyological Research, 62, 396–408.
Kido, K. (1984) Cyclopteridae. In: Masuda, H., Amaoka, K., Araga, C., Uyeno, T. & Yoshino, T. (Eds.), The fishes of the Japanese Archipelago. Tokai University Press, Tokyo, pp. 336–337.
Kim, B.J. (2015) New record of a lumpfish, Lethotremus awae (Scorpaeniformes: Cycolopteridae) form as a filling of distributional gap in the western North Pacific. Korean Journal of Ichthyology, 27, 153–158.
Kimura, M. (1980) A simple methods for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120.
Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution. [in press]
Li, C., Ortí, G., Zhang, G. & Lu, G. (2007) A practical approach to phylogenomics: the phylogeny of ray-finned fish (Actinopterygii) as a case study. BMC Evolutionary Biology, 7 (44), 1–11.
Li, S.C. (1955) Cottoidei. In: Tchang, T.L., Cheng, C.T., Cheng, P.S., Li, S.C., Cheng, W.L. & Wang, W.P. (Eds.), Fishes of the Yellow Sea and Pohai, China. Science Press, Beijing, pp. 260–272.
Lindberg, G.U. & Legeza, M.I. (1955) A review of the genera and species of the subfamily Cyclopterinae (Pisces). Trudy Zoologicheskogo Instituta, Akademii Nauk SSSR, 18, 389–458.
Masuda, H., Amaoka, K., Araga, C., Uyeno, T. & Yoshino, T. (1984) The fishes of the Japanese Archipelago. Tokai University Press, Tokyo, 437 pp.
Mecklenburg, C.W. & Sheiko, B.A. (2003) Family Cyclopteridae Bonaparte 1831-lumpsuckers. California Academy of Sciences, Annotated Checklists of Fishes, 6, 1–17.
Nagamasu, K. (2009) By-catch discards from a bottom trawl in the East China Sea. Journal of National Fisheries University, 58, 73–82.
Nakabo, T. (1993) Family Cyclopteridae. In: Nakabo, T. (Ed.), Fishes of Japan with Pictorial Keys to the Species, First Edition. Tokai University Press, Kanagawa, pp. 577–579, 1304.
Nakabo, T. (2000) Family Cyclopteridae. In: Nakabo, T. (Ed.), Fishes of Japan with Pictorial Keys to the Species. 2nd Edition. Tokai University Press, Kanagawa, pp. 662–664, 1536.
Nakabo, T. (2002) Family Cyclopteridae. In: Nakabo, T. (Ed.), Fishes of Japan with Pictorial Keys to the Species. English Edition. Tokai University Press, Kanagawa, pp. 662–664, 1529.
Nakabo, T & Kai, Y. (2013) Family Cyclopteridae. In: Nakabo, T. (Ed.), Fishes of Japan with Pictorial Keys to the Species. 3rd Edition. Tokai University Press, Kanagawa, pp. 1202–1204, 2071–2072.
Oku, K., Imamura, H. & Yabe, M. (2017) Phylogenetic relationships and a new classification of the family Cyclopteridae (Perciformes: Cottoidei). Zootaxa, 4222 (1), 1–59.
Pallas, P.S. (1769) Spicilegia Zoologica. Quibus novae imprimis et obscurae animalium species. Tomus 1. Fasciculos 7. Gottlieb August Lange, Berolini (= Berlin), 42 pp., 6 pls. [See comment under Gilbert (1896), lines 514–16]
Palumbi, S.R., Martin, A., Romano, S., McMillan, W.O., Stice, L. & Grabowski, G. (1991) The Simple Fool’s Guide to PCR. University of Hawaii Press, Honolulu, 45 pp.
Parin, N.V., Evseenko, S.A. & Vasil’eva, E.D. (2014) Fishes of Russian Seas, Annotated Catalogue. In: Proceedings of the Zoological Museum of the Moscow State University. Vol. 53. KMK Scientific Press, Moscow, 733 pp.
Parin, N.V., Fedorov, V. V. & Sheiko, B.A. (2002) An annotated catalogue of fish-like vertebrates and fishes of the seas of Russia and adjacent countries. Part 2. Order Scorpaeniformes. Journal of Ichthyology, 42 (Supplement 1), S60–S135.
Popov, A.M. (1930) A short review of the fishes of the family Cyclopteridae. Annals and Magazine of Natural History, Series 10, 6, 69–76.
Shiogaki, M., Ishito, Y., Nomura, Y. & Sugimoto, T. (2004) Revised catalogue of the fishes collected from the waters of Aomori Prefecture. Bulletin of Aomori Prefecture Fisheries Research Center, 4, 39–80.
Suzuki, T, Hosokawa, M. & Hatooka, K. (2000) Catalogue of the fishes of Hyogo Prefecture, based on the specimens collected by Toshiyuki Suzuki. Special Publications from Osaka Museum of Natural History, 32, 1–143.
Suzuki, K. & Kataoka, T. (1997) Marine Fishes of Mie Prefecture, Japan. Toba Aquarium, Mie, 297 pp., 152 pls.
Tamura, K. & Nei, M. (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10, 512–526.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28, 2731–2739.
Tanabe, A.S. (2011) Kakusan4 and Aminosan: two programs for comparing nonpartitioned, proportional, and separate models for combined molecular phylogenetic analyses of multilocus sequence data. Molecular Ecology Resources, 11, 914–921.
Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680.
Ueno, T. (1970) Fauna Japonica. Cyclopteridae (Pisces). Academic Press of Japan, Tokyo, 233 pp.
Ward, R.D., Zemlak, T.S., Innes, B.H., Last, P.R. & Hebert, P.D.N. (2005) DNA barcoding Australia’s fish species. Philosophical Transactions of the Royal Society, 360, 1847–1857.
Yamasaki, Y., Nakashima, H. & Kuwakubo, A. (2013) Record of the coast lumpfish (Lethotremus awae) incidentally captured on the coast of Hirado Takushima, Nagasaki, Japan. Transactions Nagasaki Biological Society, 72, 20–22.