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Article
Published: 2017-06-02

Comparative morphological and molecular taxonomic study of six Achaeta species (Clitellata: Enchytraeidae) with the description of a new Achaeta species from Kőszeg Mountains, Hungary

Department of Systematic Zoology and Ecology, Eötvös Loránd University H-1117 Budapest, Pázmány Péter sétány 1/C, Hungary.
Department of Microbiology, Eötvös Loránd University H-1117 Budapest, Pázmány Péter sétány 1/C, Hungary.
Annelida Enchytraeidae genus Achaeta new species molecular and morphological taxonomy

Abstract

Five Achaeta species (A. affinis, A. bohemica sensu stricto, A. camerani, A. cf. danica, A. unibulba) and a new species, described here as A. tothi sp. n., were found during the investigation of the enchytraeid fauna of western Hungary (Őrség National Park and Kőszeg Mts.). Comparative morphological and molecular taxonomic investigations were performed with several individuals representing these six Achaeta species. A detailed description of the new species is given, and we also present some additional morphological data and photos about the other five Achaeta species. Such information could have importance in subsequent taxonomic studies and will aid the discrimination among the members of genus Achaeta. Furthermore, the obtained sequences could serve as references in forthcoming studies applying DNA-based taxon identification.

 

References

  1. Benson, D.A., Cavanaugh, M., Clark, K., Karsch-Mizrachi, I., Lipman, D.J., Ostell, J. & Sayers, E.W. (2013) GenBank. Nucleic Acids Research, 41, D36–D42.

    https://doi.org/10.1093/nar/gks1195

    Černosvitov, L. (1945) Oligochaeta from Windermere and the Lake District. Proceedings of the Zoological Society of London, 114, 523–548.
    https://doi.org/10.1111/j.1096-3642.1945.tb00241.x

    Chalupský, J. (1992) Terrestrial Enchytraeidae (Oligochaeta) and Parergodrilidae (Polychaeta) from Sweden, with description of a new enchytraeid species. Zoologica Scripta, 21, 133–150.
    https://doi.org/10.1111/j.1463-6409.1992.tb00316.x

    Christensen, B. & Glenner, H. (2010) Molecular phylogeny of Enchytraeidae (Oligochaeta) indicates separate invasions of the terrestrial environment. Journal of Zoological Systematics and Evolutionary Research, 48, 208–212.

    https://doi.org/10.1111/j.1439-0469.2009.00558.x

    Cognetti, L. (1899) Descrizione dell’ Anachaeta camerani, nuova specie della famiglia degli Enchitreidi. Bolletino dei Musei di Zoologia ed Anatomia comparata della Reale Universitá di Torino, 14 (354), 1–4.

    Colgan, D.J., McLauchlan, A.A., Wilson, G.D.F., Livingston, S.P., Edgecombe, G.D., Macaranas, J., Cassis, G. & Gray, M.R. (1998) Histone H3 and U2 snRNA DNA sequences and arthropod molecular evolution. Australian Journal of Zoology, 46, 419–437.

    https://doi.org/10.1071/ZO98048

    Dózsa-Farkas, K. (2007) Comparative enchytraeid faunistic investigation of the northeastern mountain range in Hungary. Contributions to Soil Zoology in Central Europe II. In: Tajovsky, K., Schlaghamersky, J. & Pizl, V. (Eds.), Proceedings of the 8th Central European Workshop on Soil Zoology, Ceske Budejovice, Czech Republic April 20–22, 2005, pp. 29–35.

    Dózsa-Farkas, K., Felföldi, T. & Hong, Y. (2015) New enchytraeid species (Enchytraeidae, Oligochaeta) from Korea. Zootaxa, 4006 (1), 171–197.
    https://doi.org/10.11646/zootaxa.4006.1.9

    Dózsa-Farkas, K. & Felföldi, T. (2016) A new Fridericia species (Clitellata, Enchytraeidae) and the enchytraeid fauna of the Őrség National park (Hungary). Opuscula Zoologica Budapest, 47 (1), 65–72.
    https://doi.org/10.18348/opzool.2016.1.65

    Dózsa-Farkas, K., Csitári, B. & Felföldi, T. (2017) A new Cernosvitoviella species (Clitellata: Enchytraeidae) and its comparison with other Cernosvitoviella species from Sphagnum mires in Hungary. Zootaxa, 4254, 322–338.
    https://doi.org/10.11646/zootaxa.4254.3.2

    Erséus, C., Rota, E., Matamoros, L. & De Wit, P. (2010) Molecular phylogeny of Enchytraeidae (Annelida, Clitellata). Molecular Phylogenetics and Evolution, 57 (2), 849–858.

    https://doi.org/10.1016/j.ympev.2010.07.005

    Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.

    Graefe, U. (1980) Systematische Untersuchungen an der Gattung Achaeta (Enchytraeidae, Oligochaeta). I. Beschreibung von Achaeta brevivasa sp.b. und Achaeta camerani (Cognetti). Mitteilungen aus den hamburgischen zoologischen Muzeum und Institut, 77, 35–39.

    Graefe, U., Dózsa-Farkas, K. & Christensen, B. (2005) Achaeta unibulba sp. n. widespread European species (Oligochaeta, Enchytraeidae). Proceedings of the Estonian Academy of Sciences: Biology, Ecology, 54, 271–278.

    Healy, B. (1980) Records of Enchytraeidae (Oligochaeta) from western France and the Pyrénées. Bulletin du Muséum National d’Histoire Naturelle, Paris, Série 4, 2, 421–443.

    Heck, M. & Römbke, J. (1991) Two new species of Achaeta (Enchytraeidae, Oligochaeta) from meadow and pasture soils of Germany. Zoologica Scripta, 20, 215–220.
    https://doi.org/10.1111/j.1463-6409.1991.tb00287.x

    Kane, R.A. & Rollinson, D. (1994) Repetitive sequences in the ribosomal DNA internal transcribed spacer of Schistosoma haematobium, Schistosoma intercalatum and Schistosoma mattheii. Molecular and Biochemical Parasitology, 63, 153–156.

    https://doi.org/10.1016/0166-6851(94)90018-3

    Kasprzak, K. (1972) Materialy do znajomości skaposzczetów (Ologochaeta) Wielkopolski. Fragmenta faunistica, Warsawa, 18 (6), 99–119.
    https://doi.org/10.3161/00159301FF1972.18.6.099

    Kasprzak, K. (1986) Skaposzczety wodne I glebowe, II. Rodzina: Wazonkowce (Enchytraeidae). Panstwowe wydawnietwo naukowe, Warszawa, 366 pp.

    Michaelsen, W. (1889) Synopsis der Enchytraeiden. Abbhandlungen aus dem Gebiete der Naturwissenschaften, herausgegeben vom Naturwissenschaftlichen Verein in Hamburg, 11, 1–59.

    Michaelsen, W. (1900) Oligochaeta. Das Tierreich, 10, 1–575.

    Möller, F. (1971) Systematische Untersuchungen an terricolen Enchytraeiden einiger Grünlandstandorte im Bezirk Potsdam. Mitteilungen aus dem Zoologischen Museum in Berlin, 47, 131–167.
    https://doi.org/10.1002/mmnz.19710470114

    Nielsen, C.O. & Christensen, B. (1959) The Enchytraeidae. Critical revision and taxonomy of European species (Studies on Enchytraeidae VII). Natura Jutlandica, 8–9, 1–160.

    O’Connor, F.B. (1962) The extraction of Enchytraeidae from soil. In: Murphy, P.W. (Ed.), Progress in Soil Zoology, Butterworths Publishers, London, pp. 279–285.

    Rota, E. (1995) Italian Enchytraeidae (Oligochaeta). I. Bollettino di Zoologia, 62, 183–231.
    https://doi.org/10.1080/11250009509356067

    Rota, E. & Healy, B. (1994) The enchytraeid fauna of North Africa. Hydrobiologia, 278, 53–66.
    https://doi.org/10.1007/BF00142311

    Rota, E. (2015) Five new species of Enchytraeidae (Annelida: Clitellata) from Mediterranean woodlands of Italy and reaffirmed validiy of Achaeta etrusca, Fridericia bulbosa and F. miraflores. Journal of Natural History, 49 (33), 1–34.
    https://doi.org/10.1080/00222933.2015.1009514

    Schmelz, R.M., Collado, R. & Römbke, J. (2008) Mata Atlântica enchytraeids (Paraná, Brazil): The genus Achaeta (Oligochaeta, Enchytraeidae), Zootaxa, 1809, 1–35.

    Schmelz, R.M. & Collado, R. (2010) A guide to European terrestrial and freshwater species of Enchytraeidae (Oligochaeta). Soil Organisms, 82 (1), 1–176.

    Southern, R. (1913) Clare Island Survey Part 48. Oligochaeta. Proceedings of the Royal Irish Academy, 27, B (8), 119–182.

    Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.

    https://doi.org/10.1093/molbev/mst197

    Vejdovský, F. (1879) Vorläufige Mitteilungen über die fortgesetzten Oligochaetenstudien. Zoologischer Anzeiger, 2, 183–185.

    Wilcke D.E. (1967) Oligochaeta. In: Brohmer, P., Ehrmann, P. & Ulmer, G. (Eds.), Die Tierwelt Mitteleuropas. Band I. Lief 7a. Quelle & Meyer, Leipzig, pp. 1–160.

    Zicsi, A., Dózsa-Farkas, K. & Csuzdi, Cs. (1990) Terrestrial Oligochaeta from the nature conservation areas of Bátorliget (NE Hungary). In: Mahunka, S. (Ed.), Bátorliget Nature Reserves – after forty years, 1, pp. 215–220.