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Type: Article
Published: 2016-10-25
Page range: 347–370
Abstract views: 507
PDF downloaded: 97

Different continents, same species? Resolving the taxonomy of some Holarctic Ancylis Hübner (Lepidoptera: Tortricidae)

Identification Technology Program, USDA-APHIS-PPQ-S&T, 2301 Research Blvd., Suite 108, Fort Collins, Colorado 80526, USA.
Tiroler Landesmuseen Betriebgsges.m.b.H., Naturwissenschaftliche Sammlungen, Feldstr. 11a, A-6020 Innsbruck, Austria.
Tiroler Landesmuseen Betriebgsges.m.b.H., Naturwissenschaftliche Sammlungen, Feldstr. 11a, A-6020 Innsbruck, Austria.
Lepidoptera christiandiana DNA barcoding Enarmoniini oregonensis saliana


Several species of Ancylis related to A. unguicella (Linnaeus) and A. geminana (Donovan) have been presumed by previous authors to be Holarctic. However, difficulty in identifying genitalic characters to define and separate these taxa has brought into question their true distribution and led to inconsistencies in their taxonomic treatment in Europe and North America. Here we use a combination of DNA barcode sequence data and morphology to resolve these taxonomic differences, determine the actual geographic range of these taxa, and describe three new species. In the A. unguicella group, only A. unguicella and A. uncella (Denis & Schiffermüller) are Holarctic in distribution. In the A. geminana group, none of the taxa are Holarctic in their distribution. Three species are described as new: A. christiandiana Huemer and Wiesmair, sp.n.  (Austria, Germany); A. oregonensis Gilligan and Huemer, sp.n.  (USA: Oregon); and A. saliana Gilligan and Huemer, sp.n.  (USA: Florida). In addition, Ancylis carbonana Heinrich, syn.n., is synonymized with A. uncella; A. cuspidana (Treitschke), syn.rev., is synonymized with A. diminutana (Haworth); and A. diminuatana Kearfott, stat.rev., and A. subarcuana (Douglas), stat.rev., are raised from synonymy.



  1. Aarvik, L.E. (2013) Fauna Europaea: Tortricidae. In: Karsholt, O. & Nieukerken, E.J. van (Eds.), Fauna Europaea: Lepidoptera, Moths. Fauna Europaea version 2.6. Available from: (Accessed 25 Oct. 2016)

    Arnqvist, G. (1997) The evolution of animal genitalia: distinguishing between hypotheses by single species studies. Biological Journal of the Linnean Society, 60, 365–379.

    Barnes, W. & McDunnough, J. (1917) Checklist of the Lepidoptera of Boreal America. Decatur, Illinois. 200 pp.

    Bradley, J.D., Tremewan, W.G. & Smith, A. (1979) British Tortricoid Moths. Tortricidae: Olethreutinae. The Ray Society, London. Viii + 336 pp, pls 22–43.

    Brown, J.W. (2005) World catalogue of insects. Volume 5: Tortricidae (Lepidoptera). Apollo Books, Stenstrup, Denmark. 741 pp.

    Brown, J.W. & Powell, J.A. (1991) Systematics of the Chrysoxena group of genera (Lepidoptera: Tortricidae: Euliini). University of California Publications in Entomology, 111, 87 pp.

    Brown, J.W., Janzen, D., Hallwachs, W., Zahiri, R., Hajibabaei, M. & Hebert, P.N.D. (2014) Cracking complex taxonomy of Costa Rican moths: Anacrusis Zeller (Lepidoptera: Tortricidae). Journal of the Lepidopterists’ Society, 68, 248–263.

    deWaard, J.R., Ivanova, N.V., Hajibabaei, M. & Hebert, P.D.N. (2008) Assembling DNA Barcodes: Analytical Protocols. In: Cristofre, M, (Ed.), Methods in Molecular Biology: Environmental Genetics. Humana Press Inc., Totowa, USA. pp. 275–293.

    Donovan, E. (1806) The Natural History of British Insects: Explaining them in their several states, with the periods of their transformations, their food, oeconomy, etc. Together with the history of such minute insects as require investigation by the microscope: the whole illustrated by coloured figures, designed and executed from living specimens. Volume 11. pp. 361–396.

    Eberhard, W.G. (1985) Sexual selection and animal genitalia. Harvard University Press, Cambridge, Massachusetts. 244 pp.

    Fernald, C.H. (1882) A synonymical catalogue of the described Tortricidae of North America north of Mexico. Transactions of the American Entomological Society, 10, 1–64.

    Fernald, C.H. (1903 [1902]) Family Tortricidae. In: Dyar, H.G. (Ed.), A list of North American Lepidoptera. Bulletin of the United States National Museum, No. 52. pp. 448–489.

    Gilligan, T.M. & Wenzel, J.W. (2008) Extreme intraspecific variation in Hystrichophora (Lepidoptera: Tortricidae) genitalia —questioning the lock-and-key hypothesis. Annales Zoologici Fennici, 45, 465–477.

    Gilligan, T.M., Wright, D.J. & Gibson, L.D. (2008) Olethreutine moths of the midwestern United States, an identification guide. Bulletin of the Ohio Biological Survey, Volume 16, 334 pp.

    Gilligan, T.M., Baixeras, J., Brown, J.W. & Tuck, K.R. (2014a) T@RTS: Online World Catalogue of the Tortricidae (Ver. 3.0). Available from: (Accessed 25 Oct. 2016)

    Gilligan, T.M., Wright, D.J., Munz, J., Yakobson, K. & Simmons, M.P. (2014b) Molecular phylogeny and revised classification of Eucosma Hübner and related genera (Lepidoptera: Tortricidae: Eucosmini). Systematic Entomology, 39, 49–67.

    Hebert, P.D.N., Cywinska, A., Ball, S.L. & de Waard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London, B, 270, 313–321.

    Heinrich, C. (1923) Revision of the North American moths of the subfamily Eucosminae of the family Olethreutidae. Bulletin of the United States National Museum, 123, 1–128.

    Heinrich, C. (1926) Revision of the North American moths of the subfamilies Laspeyresiinae and Olethreutinae. Bulletin of the United States National Museum, 132, 1–216.

    Horak, M. (1984) Assessment of taxonomically significant structures in the Tortricinae (Lep.: Tortricidae). Mitteilungen der schweizerischen entomologischen Gesellschaft, 57, 3–64.

    Horak, M. (1999) The Tortricoidea, In: Kristensen, N.P. (Ed.), Lepidoptera: Moths and Butterflies. Volume 1: Evolution, systematics, and biogeography. Handbook of Zoology Vol. IV, Part 35. Walter de Gruyter, Berlin and New York. pp. 199–215.

    Horak, M. (2006) Olethreutine moths of Australia (Lepidoptera: Tortricidae). Monographs on Australian Lepidoptera, 10, 1–522.

    Hosken, D.J. & Stockley, P. (2004) Sexual selection and genital evolution. Trends in Ecology & Evolution, 19, 87–93.

    Humble, L.M., deWaard, J.R. & Quinn, M. (2009) Delayed recognition of the European poplar shoot borer, Gypsonoma aceriana (Duponchel) (Lepidoptera: Tortricidae) in Canada. Journal of the Entomological Society of British Columbia, 106, 61–70.

    Kearfott, W.D. (1905) Descriptions of new species of tortricid moths from North Carolina, with notes. Proceedings of the United States National Museum, 28, 349–364.

    Klots, A.B. (1970) Lepidoptera. In: Tuxen, S.L. (Ed), Taxonomic Glossary of Genitalia in Insects. Munksgaard, Copenhagen. pp. 115–130.

    Landry, J.F., Nazari, V., deWaard, J.R., Mutanen, M., Lopez-Vaamonde, C., Huemer, P. & Hebert, P.D.N. (2013) Shared but overlooked: 30 species of Holarctic Microlepidoptera revealed by DNA barcodes and morphology. Zootaxa, 3749 (1), 1–93.

    Mikkola, K. (1992) Evidence for lock-and-key mechanisms in the internal genitalia of the Apamea moths (Lepidoptera, Noctuidae). Systematic Entomology, 17, 145–153.

    Miller, W.E. (1987) Guide to the olethreutine moths of Midland North America (Tortricidae). United States Department of Agriculture. Forest Service Agriculture Handbook, 660. 104 pp.

    Mutanen, M., Rytkonen, S., Linden, J. & Sinkkonen, J. (2007) Male genitalia variation in a moth Pammene luedersiana (Lepidoptera: Tortricidae). European Journal of Entomology, 104, 259–265.

    Mutanen, M., Aarvik, L. Landry, J.-F., Segerer, A.H. & Karsholt, O. (2012) Epinotia cinereana (Haworth, 1811) bona sp., a Holarctic tortricid distinct from E. nisella (Clerck, 1759) (Lepidoptera: Tortricidae: Eucosmini) as evidenced by DNA barcodes, morphology and life history. Zootaxa, 3318, 1–25.

    Pierce, F.N. & Metcalfe, J.W. (1922) The genitalia of the group Tortricidae of the Lepidoptera of the British Islands. Oundle, Liverpool, England. 101 pp.

    Powell, J.A. (1983) Tortricidae, In: Hodges, R.W. (Ed.), Check list of the Lepidoptera of America north of Mexico. E.W. Classey & Wedge Entomological Research Foundation, London, pp. 31–41.

    Powell, J.A. & Opler, P.A. (2009) Moths of Western North America. University of California Press. Berkeley, Los Angeles, London. 369 pp.

    Ratnasingham, S. & Hebert, P.D.N. (2007) BOLD: The Barcode of Life Data System ( Molecular Ecology Notes, 7, 355–364.

    Razowski, J. (1989) The genera of Tortricidae (Lepidoptera). Part II: Palaearctic Olethreutinae. Acta Zoologica Cracoviensia, 32, 107–328.

    Razowski, J. (1996) Tortricidae, In: Karsholt, O. & Razowski, J. (Eds.), The Lepidoptera of Europe. A distributional checklist. Apollo Books, Stenstrup. pp. 130–157, 313–318.

    Razowski, J. (2001) Die Tortriciden (Lepidoptera, Tortricidae) Mitteleuropas. Bestimmung – Verbreitung – Flugstandort – Lebensweise der Raupen. František Slamka, Bratislava. 301 pp.

    Razowski, J. (2003) Tortricidae of Europe, Volume 2, Olethreutinae. František Slamka, Bratislava. 301 pp.

    Rebel, H. (1901) Catalog der Lepidopteren des palaearctischen Faunengebietes. II. Theil: Famil. Pyralidae-Micropterigidae. R. Friedländer & Sohn, Berlin. 368 pp.

    Schütze, K.T. (1931) Die Biologie der Kleinschmetterlinge unter besonderer Berücksichtigung ihrer Nährpflanzen und Erscheinungszeiten. Internationaler Entomologischer Verein, Frankfurt. 235 pp.

    Sinev, S.Yu. (Ed.) (2008) Catalogue of the Lepidoptera of Russia. KMK Scientific Press, St. Petersburg, Moscow. 424 pp.

    Stephens, J.F. (1829) A systematic catalogue of British insects: being an attempt to arrange all the hitherto discovered indigenous insects in accordance with their natural affinities. London: Published for the author, by Baldwin and Cradock. 852 pp.

    Walsingham, L.T. de G. (1897) Revision of the West-Indian Microlepidoptera with descriptions of new species. Proceedings of the Entomological Society of London, 1897, 54–183.

    Wegner, H. (2015) Ein Beitrag zur Wickler-Fauna in Nordost-Niedersachsen und in Schleswig-Holstein (Lep., Tortricidae). Melanargia, 27 (4), 137–154.

    Wocke, M. (1871) In: Staudinger, O. & Wocke, M. (1871), Catalog der Lepidopteren des europäischen Faunengebiets. Dresden. 426 pp.

    Wright, D.J. & Gilligan, T.M. (2015) Eucosma Hübner of the Contiguous United States and Canada (Lepidoptera: Tortricidae: Eucosmini). Wedge Entomological Research Foundation, Alamogordo, New Mexico, 256 pp.