Abstract
Aromobates is a genus endemic to the northern Andes of Colombia and Venezuela. Limited information is available aboutthis genus diversity, current distribution, and taxonomic characterization. Here, we describe three new species of Aromo-bates from the Andes of Venezuela. We provide the most comprehensive phylogeny of Aromobates to date and taxonomicnotes on Aromobates saltuensis Rivero and A. inflexus Rivero. We also discuss the limitations of the current molecularand morphological diagnosis of Aromobates. Finally, we comment on the conservation status and research priorities for this genus.References
Anganoy-Criollo, M. (2012) A new species of Allobates (Anura, Dendrobatidae) from the western flank of the Serranía de Perijá, Colombia. Zootaxa, 3308, 49–62.
Barrio-Amorós. C.L. (1998) Sistemática y Biogeografía de los anfibios (Amphibia) de Venezuela. Acta Biologica Venezuelica, 18, 1–93.
Barrio-Amorós. C.L. (2001) State of knowledge on the declination of amphibians in Venezuela. FROGLOG, 47, 2–4.
Barrio-Amorós. C.L. (2004) Amphibians of Venezuela, Systematic list, Distribution and References; an Update. Revista de Ecologia Latino Americana, 9, 1–48.
Barrio-Amorós. C.L. (2009a) Riqueza y Endemismo. Pp. 25–39 in Molina, C. Señaris, J. C. Lampo, M. & Rial, A. (eds.), Anfibios de Venezuela, estado del conocimiento y recomendaciones para su conservación. Ediciones Grupo TEI, Caracas.
Barrio-Amorós. C.L. (2009b) Evaluación poblacional y de salud del sapito arlequín de Mucubají (Atelopus mucubajiensis) en el Parque Nacional Sierra Nevada, estado Mérida. Pp. 177 In Giraldo, D., Rojas-Suarez, F. & Romero, V. (eds.). Una mano a la Naturaleza, conservando las especiesamenazadas venezolanas. Provita & Shell Venezuela, S.A., Caracas.
Barrio-Amorós. C.L. (2009c) Estatus de la población de la rana Aromobates meridensis (Anura: Aromobatidae) infectada por hongo, en los Andes de Venezuela. Pp. 181 in Giraldo, D., Rojas-Suarez, F., & Romero, V. (eds.). Una mano a la Naturaleza, conservando las especies amenazadas venezolanas. Provita & Shell Venezuela, S.A., Caracas.
Barrio-Amorós. C.L. (2010) Catálogo ilustrado de los anfibios y reptiles del Ramal de Calderas, Andes de Venezuela, 141–156. In: Rial, A, Señaris, J. C., Lasso, C.A. & Flores, A. (eds) (2010) Evaluación Rápida de la Biodiversidad y Aspectos Socioecosistémicos del Ramal de Calderas. Andes de Venezuela. RAP Bulletin of Biological Assesment 56. Conservation International, Arlington, VA. USA, 183 pp.
Barrio-Amorós,C.L. & Santos, J.C. (2009) Description of a new Allobates (Amphibia: Anura: Dendrobatidae) from the eastern Andean piedmont, Venezuela. Phyllomedusa, 8, 89–104.
Barrio-Amorós,C.L. Rivero, R. & Santos, J.C. (2011) A new striking dendrobatid frog (Dendrobatidae, Aromobatinae, Aromobates) from the Venezuelan Andes. Zootaxa, 3063, 39–52.
Barrio-Amorós, C.L., Romero E. & Infante, E. (2010a). The Critically Endangered Venezuelan Dendrobatid Frog Aromobates meridensis (Amphibia: Anura): redescription, natural history and conservation. Revista Latino Americana de Ecologia, 15, 1–12.
Barrio-Amorós, C.L., Santos, J.C. and Molina, C.R. (2010b) An addition to the Diversity of dendrobatid frogs in Venezuela: description of three new collared frogs (Anura: Dendrobatidae: Mannophryne). Phyllomedusa, 9, 3–35.
Barrio-Amorós,C.L., Rivas, G., Molina, C., Santos, J.C. & Kaiser,H. (2010c) Intraspecific variation in the Endangered frog Mannophryne riveroi (Anura, Dendrobatidae, Aromobatinae), with comments on coloration and natural history. Herpetological Notes, 3, 151–160.
Boulenger, G.A. (1903) On some Batrachians and Reptiles from Venezuela. Annals Magazine Natural History, 7, 481–484.
Charif, R.A., Waack, A.M. & Strickman, L.M. (2010) Raven Pro 1.4 Ithaca, NY: Cornell Lab of Ornithology.
Díaz de Gamero, M.L. (1996) The changing course of the Orinoco River during the Neogene: a review. Palaeogeography, Palaeoclimatology, Palaeoecology, 123, 385–402.
Dole, J. & Durant, P. (1972) A new species of Colostethus (Amphibia: Salientia) from the Mérida Andes, Venezuela. Caribbean Journal Science, 12, 191–193.
Funk, D.J. & Omland, K.E (2003) Species-level paraphyly and polyphyly: frequency, causes, and consequences with insights from animal mitochondrial DNA. Annual Review of Ecology, Evolution, and Systematics, 34, 397–423.
Grant, T., Humphrey, E.C. & Myers, C.W. (1997) The median lingual process of frogs: a bizarre character of old world ranoids disvovered in South American dendrobatids. American Museum Novitates, 3212, 1–40.
Grant, T., Frost, D.R. Caldwell, J.P. Gagliardo, R. Haddad, C.F.B. Kok, P.J.R. Means, D.B. Noonan, B.P. Schargel, W.E. & Wheeler W.C. (2006) Phylogenetic systematics of dart-poison frogs and their relatives (Amphibia: Athesphatanura: Dendrobatidae). Bulletin American Museum of Natural History, 299, 1–262.
Hoorn, C., Guerrero J., Sarmiento G. A, & Lorente, M.A. (1995) Andean tectonics as a cause for changing drainage patterns in Miocene northern South America. Geology, 23, 237–240.
Huelsenbeck, J. P. & Ronquist, F. P. (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17, 754–755.
La Marca, E. (1985) A new species of Colostethus (Anura: Dendrobatidae) from the Cordillera de Mérida, Northern Andes, South America. Occasional Papers Museum Zoology University Michigan, 710, 1–10.
La Marca, E. (1991a "1994") Descripción de un género nuevo de ranas (Amphibia: Dendrobatidae) de la cordillera de Mérida, Venezuela. Anuario de Investigación 1991, Instituto de Geografia U.L.A., 39–41.
La Marca, E. (2005) Estatus de poblaciones de ranas de la familia Dendrobatidae (Amphibia: Anura) en sus localidades tipo en los Andes de Venezuela. Herpetotropicos, 2, 73–86.
La Marca, E., Vences, M. & Lötters, S. (2002) Rediscovery and mitochondrial relationships of the dendrobatid frog Colostethus humilis suggest parallel colonization of the Venezuelan Andes by poison frogs. Studies Neotropical Fauna Environment, 37, 233–240.
Liu, K., Raghavan, S. Nelesen, S. Linder, C.R. & Warnow, T. (2009) Rapid and accurate large-scale coestimation of sequence alignments and phylogenetic trees. Science, 324, 1561–1564.
Lötters, S., Jungfer, K.H., Henkel, F.W. & Schmidt, W. (2007) Poison Frogs. Biology, Species & Captive Husbandry. Edition Chimaira, Frankfurt am Main.
Manzanilla, J., La Marca, E. & García-Paris, M. (2009) Phylogenetic patterns of diversification in a clade of Neotropical frogs (Anura: Aromobatidae: Mannophryne). Biological Journal Linnean Society, 97, 185–199.
Mijares-Urrutia, A. & La Marca, E. (1997) Tadpoles of the genus Nephelobates La Marca,1994 (Amphibia, Anura, Dendrobatidae) from Venezuela. Tropical Zoology, 10, 133–142.
Morrison, D. A., & Ellis, J. T. (1997) Effects of nucleotide sequence alignment on phylogeny estimation: A case study of 18S rDNAs of Apicomplexa. Molecular Biology and Evolution, 14, 428–441.
Myers, C.W. & Duellman, W.E. (1982) A new species of Hyla from Cerro Colorado, and other tree frog records and geographical notes from western Panama. American Museum Novitates, 2752, 1–32.
Myers, C.W., Paolillo, A. & Daly, J.W. (1991) Discovery of a defensively malodorous and nocturnal frog in the family Dendrobatidae: phylogenetic significances of a new genus and species from the Venezuelan Andes. American Museum Novitates, 3002, 1–33.
Ogden, T. H., & Rosenberg, M. S. (2007) Alignment and topological accuracy of the direct optimization approach via POY and traditional phylogenetics via ClustalW plus PAUP*. Systematic Biology, 56, 182–193.
Péfaur, J.E. (1985) New species of Venezuelan Colostethus (Dendrobatidae). Journal of Herpetology, 19, 321–327.
Péfaur, J.E. (1993) Description of a new Colostethus (Dendrobatidae) with some natural history on the genus in Venezuela. Alytes, 11, 88–96.
Piñero, J., & La Marca, E. (1996) Hábitos alimentarios de Nephelobates alboguttatus (Anura: Dendrobatidae) en una selva nublada andina de Venezuela. Revista Biologia Tropical, 44, 827–833.
Posada, D. (2008) jModelTest: Phylogenetic Model Averaging. Molecular Biology and Evolution, 25, 1253–1256.
Rambaut, A., & Drummond A. J. (2007) Tracer v1.4. Available from http://beast.bio.ed.ac.uk/Tracer
Rivero, J.A. (1961) Salientia of Venezuela. Bulletin of the Museum of Comparative Zoology, 126, 1–267.
Rivero, J.A. (1976) Notas sobre los anfibios de Venezuela II. Sobre los Colostethus de los Andes Venezolanos.Memoria. Sociedad de Ciencias Naturales La Salle, 35, 327–344.
Rivero, J.A. (1978) Notas sobre los anfibios de Venezuela III. Nuevos Colostethus de los Andes Venezolanos.Memoria. Sociedad de Ciencias Naturales La Salle, 38, 95–111.
Rivero, J.A. (1984 "1982") Sobre el Colostethus mandelorum (Schmidt) y el Colostethus inflexus Rivero (Amphibia, Dendrobatidae). Memoria. Sociedad de Ciencias Naturales La Salle, 42, 9–16.
Rivero, J.A. (1988) Sobre las relaciones de las especies del género Colostethus (Amphibia, Dendrobatidae).Memoria. Sociedad de Ciencias Naturales La Salle, 48, 3–32.
Rojas-Runjaic, F., Infante-Rivero, E. & Barrio-Amorós, C.L. (2011) A new frog of the genus Aromobates (Anura, Dendrobatidae) from Sierra de Perijá, Venezuela. Zootaxa, 2919, 37–50.
Santos, J.C. (2012) Fast molecular evolution associated with high active metabolic rates in poison frogs. Molecular Biology Evolution, first published online February 15, 2012 doi:10.1093/molbev/mss069
Santos, J.C. & Cannatella, D.C. (2011) Phenotypic integration emerges from aposematism and scale in poison frogs. Proceedings of the Natural Academy of Sciences, USA, 108, 6175–6180.
Santos J.C, Coloma, L.A., Summers, K., Caldwell, J.P. & Ree, R. (2009) Amazonian amphibian diversity is primarily derived from late Miocene Andean lineages. PLoS Biol, 7, e1000056. doi:10.1371/journal.pbio.1000056, 1–14.
Schloss, P. D. (2010) The effects of alignment quality, distance calculation method, sequence filtering, and region on the analysis of 16S rRNA gene-based studies. Plos Computational Biology 6, e100084410.1371/journal.pcbi.1000844.
Stamatakis, A. (2006) RAxML-VI-HPC: Maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics, 22, 2688–2690.
Stuart, S.N., Hoffman, M., Chanson, J., Cox, N., Berridge, R., Ramani, P. & Young, B. (eds.) (2008) Threatened Amphibians of the World. Lynx Editions, Barcelona, Spain; IUCN, Gland. Switzerland; Conservation International, Arlington, Virginia, U.S.A.
Strugnell, J., & Nishiguchi, M. K. (2007) Molecular phylogeny of coleoid cephalopods (Mollusca : Cephalopoda) inferred from three mitochondrial and six nuclear loci: A comparison of alignment, implied alignment and analysis methods. Journal of Molluscan Studies, 73, 399–410.
Swofford, D. L. (2000) PAUP* Phylogenetic analysis using parsimony (*and other methods). Sinauer Associates, Sunderland (MA).
Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin, and D. Higgins. (1997) The Clustal X window interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876–4882.
Vences, M., Kosuch, J., Boistel, R., Haddad, C., La Marca, E., Lötters, S. & Veith, M. (2003) Convergent evolution of aposematic coloration in Neotropical poison frogs: a molecular phylogenetic perspective. Organism Diversity Evolution 3, 215–226.