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Published: 2012-05-30

Description of three Rhacophorus tadpoles (Lissamphibia: Anura: Rhacophoridae) from Sarawak, Malaysia (Borneo)

Zoologisches Museum Hamburg, Martin-Luther-King-Platz 3, 20146 Hamburg, Germany
Naturhistorisches Museum der Burgergemeinde Bern, Bernastrasse 15, CH-3005 Bern, Switzerland
Zoologisches Museum Hamburg, Martin-Luther-King-Platz 3, 20146 Hamburg, Germany
Zoologisches Museum Hamburg, Martin-Luther-King-Platz 3, 20146 Hamburg, Germany
Institut für Integrierte Naturwissenschaften, Abteilung Biologie, Universität Koblenz-Landau, Universitätsstraße 1, 56070 Koblenz, Germany
Institute of Biodiversity and Environmental Conservation, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
Zoologisches Museum Hamburg, Martin-Luther-King-Platz 3, 20146 Hamburg, Germany
Naturhistorisches Museum der Burgergemeinde Bern, Bernastrasse 15, CH-3005 Bern, Switzerland
Institute of Biodiversity and Environmental Conservation, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
Amphibia Rhacophorus rufipes R. penanorum R. dulitensis tadpole description larval morphology oral disc rheophilous tadpole DNA barcoding


This communication reports the discovery of the hitherto unknown larval forms of Rhacophorus rufipes and R. penanorum, andre-describes the tadpole of R. dulitensis. Tadpoles of all three species were discovered at Gunung Mulu National Park, Sarawak(Borneo), Malaysia. The identity of the larvae was determined by DNA barcoding techniques using partial 16S rRNA mito-chondrial gene sequences. Larval DNA sequences matched those of syntopic adults of respective species. Detailed descriptionsof external morphology and colouration in life are provided along with ecological notes. The tadpole of R. rufipes and R.dulitensis can be classified as generalized, benthic-nectonic type, whereas tadpoles of R. penanorum show adaptations typical for a lotic, rheophilous lifestyle.


  1. Altig, R. (2007) A primer for the morphology of anuran tadpoles. Herpetological Conservation and Biology, 2, 71–74.

    Altig, R. & Johnston, G.F. (1989) Guilds of anuran larvae: relationships among developmental modes, morphologies, and habitats. Herpetological Monographs, 3, 81–109.

    Altig, R. & McDiarmid, R.W. (1999) Body plan: development and morphology. In: McDiarmid, R.W. & Altig, R. (Eds), Tadpoles: The biology of anuran larvae. University of Chicago Press, pp. 24–51.

    AmphibiaWeb (2010) AmphibiaWeb: Information on amphibian biology and conservation. University of California, Berkeley. Electronically accessible at:

    Anstis, M.(2002) The tadpoles of South-eastern Australia. New Holland Publishers (Australia), Sydney, Auckland, London, Cape Town, 281 pp.

    Boulenger, G.A. (1892) An account of the reptiles and batrachians collected by Mr. C. Hose on Mt. Dulit, Borneo. Proceedings of the Zoological Society London, 1892, 505–508

    Chou, W. & Lin, J. (1997) Tadpoles of Taiwan. Special Publication National Museum of Natural Science, 7, 1–98.

    Das, I. & Haas, A. (2005) Sources of larval identities for amphibians from Borneo. Herpetological Review, 36, 375–382.

    Das, I. & Haas, A. (2011) Scientific results of the Volkswagen Stiftung Bornean amphibian biodiversity project. In Das , I., Haas, A. & Tuen, A.A. (Eds.) Biology of the Amphibians in the Sunda Region, South-east Asia. Institute of Biodiversity and Environmental Conservation, Universiti Malaysia Sarawak, Kota Samarahan. pp 8–25.

    Dehling, J.M. (2008) A new treefrog (Anura: Rhacophoridae: Rhacophorus) from Gunung Mulu, Borneo. Salamandra, 44, 193–205.

    Dehling, M. & Grafe, U. (2008) A new treefrog of the genus Rhacophorus (Anura: Rhacophoridae) from Brunei Darussalam (Borneo). Salamandra 44, 101–112.

    Dring, J. (1983a) Frogs of the genus Leptobrachella (Pelobatidae). Amphibia-Reptilia, 4, 89–102.

    Dring, J. (1983b) Some new frogs from Sarawak. Amphibia-Reptilia, 4, 103–115.

    Frost, D.R. (2011) Amphibian Species of the World: an Online Reference. Version 5.5 (31 January 2011). American Museum of Natural History, New York. Electronically accessible at:

    Gawor, A., Hendrix, R., Vences, M., Böhme, W. & Ziegler, T. (2009) Larval morphology in four species of Hylarana from Vietnam and Thailand with comments on the taxonomy of H. nigrovittata sensu lato (Anura: Ranidae). Zootaxa, 2051, 1–25.

    Gosner, K.L. (1960) A simplified table for staging anuran embryos and larvae with notes on identification. Herpetologica, 16, 183–190.

    Gosner, K.L. & Rossman, A. (1960) Eggs and larval development of the treefrogs Hyla crucifer and Hyla ocularis. Herpetologica, 16, 225–232.

    Grafe, T.U. & Keller, A. (2008) A Bornean amphibian hotspot: the lowland mixed dipterocarp rainforest at Ulu Temburong National Park, Brunei Darussalam. Salamandra, 44, 241–254.

    Grosjean, S. (2005) The choice of external morphological characters and developmental stages for tadpole-based anuran taxonomy: a case study in Rana (Sylvirana) nigrovittata (Blyth, 1855) (Amphibia, Anura, Ranidae). Contributions to Zoology, 74, 61–76.

    Haas, A. & Das. I. (2011) Describing East Malaysia tadpole diversity: Status and recommendations for standards and procedures associated with larval amphibian description and documentation. Bonner Zoologische Monographien 57, 129–146.

    Hall T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic acids symposium series, 41, 95-98.

    Hertwig, S.T., Das, I., Schweizer, M., Brown, R.M. & Haas, A. (2011) Phylogenetic relationships of the Rhacophorus everetti-group, with a description of an unique tadpole and implications for the evolution of reproductive modes in Bush Frogs (Amphibia: Anura: Rhacophoridae: Philautus). Zoologica Scripta 41, 29–46

    Inger, R.F. (1966) The systematics and zoogeography of the Amphibia of Borneo. Fieldiana Zoology, 52, 1–402.

    Inger, R.F. (1983) Larvae of southeast Asian species of Leptobrachium and Leptobrachella (Anura: Pelobatidae). In: Rhodin, A. & Miyata, K. (Eds), Advances in herpetology and evolutionary biology. Museum of Comparative Zoology, Cambridge, Massachusetts, pp. 13–32.

    Inger, R.F. (1985) Tadpoles of the forested regions of Borneo. Fieldiana: Zoology, new series, 26, 1–89.

    Inger, R.F. (1999) 8. Distribution of amphibians in southern Asia and adjacent islands. In: Duellman, W.E. (Ed), Patterns of distribution of amphibians: a global perspective. Johns Hopkins University Press, pp. 445–482.

    Inger, R.F. & Stuebing, R.B. (1989) Frogs of Sabah. Sabah Parks Trustees, Kota Kinabalu, 132 pp.

    Inger, R.F., Stuebing, R.B. & Stuart, B.L. (2006) The tadpole of Rana glandulosa Boulenger (Anura: Ranidae). Raffles Bulletin of Zoology, 54, 465–467.

    Inger, R.F. & Tan, F.L. (1990) Recently discovered and newly assigned frog larvae (Ranidae and Rhacophoridae) from Borneo. Raffles Bulletin of Zoology, 38, 3–9.

    Inger, R.F., Tan, F.L. & Yambun, P. (2000) The frog fauna of three parks in Sabah, Malaysia – Kinabalu Park, Crocker Range Park, and Tawau Hills Park. Sabah Parks Nature Journal, 3, 7–28.

    Katoh, K., Misawa, K., Kuma, K. & Miyata, T. (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research, 30, 3059–3066.

    Leong, T.M. (2004) Larval descriptions of some poorly known tadpoles from Peninsular Malaysia (Amphibia: Anura). Raffles Bulletin of Zoology, 52, 609–620.

    Leong, T.M. & Chou, L. (1999) Larval diversity and development in the Singapore Anura (Amphibia). Raffles Bulletin of Zoology, 47, 81–137.

    Malkmus, R., Manthey, U., Vogel, G., Hoffmann, P. & Kosuch, J. (2002) Amphibians and Reptiles of Mount Kinabalu (North Borneo). A.R.G. Gantner K.G., Koeltz Scientific Books, Koenigstein, 424 pp.

    McDiarmid, R.W. & Altig, R. (1999) Tadpoles: the biology of anuran larvae. University Chicago Press, Chicago, London, xiv + 444 pp.

    Morrison, D.A. (2009) Why would phylogeneticists ignore computerized sequence alignment? Systematic Biology, 58, 150–158.

    Nodzenski, E. & Inger, R.F. (1990) Uncoupling of related structural changes in metamorphosing torrent-dwelling tadpoles. Copeia 1990, 1047–1054.

    Palumbi, S.R., Martin, A., Romano, S., McMillan, W.O., Stice, L. & Grabowski, G. (1991) The simple fool’s guide to PCR, version 2.0. University of Hawaii, Honolulu. 47 pp.

    Posada, D. (2008) jModelTest: phylogenetic model averaging. Molecular Biology and Evolution, 25, 1253–1256.

    Sabaj Pérez, M.H. (editor). 2012. Standard symbolic codes for institutional resource collections in herpetology and ichthyology: an online reference. Version 3.0 (23 February 2012). Electronically accessible at, American Society of Ichthyologists and Herpetologists, Washington, D.C.

    Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599.

    Vences, M., Thomas, M., Meijden, A.V.D., Chiari, Y. & Vieites, D.R. (2005) Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians. Frontiers of Zoology, 2, 5 (1–12).

How to Cite

HAAS, A., HERTWIG, S. T., KRINGS, W., BRASKAMP, E., DEHLING, J. M., MIN, P. Y., JANKOWSKI, A., SCHWEIZER, M., & DAS, I. (2012). Description of three <em>Rhacophorus </em>tadpoles (Lissamphibia: Anura: Rhacophoridae) from Sarawak, Malaysia (Borneo). Zootaxa, 3328(1), 1–19.