Abstract
The name Anopheles (Kerteszia) lepidotus Zavortink, commonly used for an important malaria vector in the eastern cor-dillera of the Andes, is here corrected to An. pholidotus Zavortink. We discovered that An. (Ker.) specimens from Peru,and reared-associated specimens from Ecuador, had unambiguous habitus characters that matched those on the male ho-lotype of An. lepidotus. However, the specimens do not exhibit characters of the female allotype and female paratypes ofAn. lepidotus, which are actually An. pholidotus. Our specimens are the first correctly associated females of An. lepidotus,which allow us to provide a new morphological interpretation for the adult habitus of this species. This finding is alsocorroborated by molecular data from a portion of the Cytochrome Oxidase I (COI) gene and ribosomal DNA Internal Tran-scribed Spacer 2 (rDNA ITS2). The pupal stage of An. lepidotus is described for the first time, and additional larval char-acters are also noted. Diagnostic morphological characters for the adult, pupal, and larval stages of An. pholidotus areprovided to separate the two species. All stages of An. lepidotus are easily separated from other currently known speciesin subgenus Kerteszia and a new key to the females of An. (Kerteszia) is given. Previously published distribution, bionomics, and medical significance data are corrected and enhanced.References
Aragão, M.B. (1964) Distribuição geográfica e abundancia das especies de Anopheles (Kerteszia) (Diptera, Culicidae). Revista Brasileira de Malariologia e Doenças Tropicais, 16, 73–109.
Bates, M. (1945) Observations on climate and seasonal distribution of mosquitoes in eastern Colombia. The Journal of Animal Ecology, 14, 17–25.
Collucci, E. & Sallum, M.A.M. (2003) Phylogenetic analysis of the subgenus Kerteszia of Anopheles (Diptera: Culicidae: Anophelinae) based on morphological characters. Insect Systematics and Evolution, 34, 361–372.
Cova-García, P., Pulido-F., J. & Escalante de Ugueto, C. (1976) Anopheles (Kerteszia) hilli (Diptera, Culicidae) una nueva specie de Venezuela. Boletín de la Dirección de Malariología y Saneamiento Ambiental, 17, 344–53.
Cova-García, P., Pulido-F., J. & Escalante de Ugueto, C. (1977a) Anopheles (Kerteszia) gonzalezrinconesi n. sp. (Diptera, Culicidae) de Venezuela. Boletín de la Dirección de Malariología y Saneamiento Ambiental, 17, 140–149.
Cova-García, P., Pulido-F., J. & Escalante de Ugueto, C. (1977b) Anopheles (Kerteszia) rollai, nomen novum Anopheles (Kerteszia) hilli Cova Garcia, 1976 (non Anopheles hilli Woodhill & Lee 1944). Boletín de la Dirección de Malariología y Saneamiento Ambiental, 17, 241.
Deane, L.M. (1967) Monkey malaria in Brazil, a summary of studies performed in 1964-1966. World Health Organization/Malaria (WHO/MAL) 67.603, 20 pp.
Deane, L.M. (1988) Malaria studies and control in Brazil. American Journal of Tropical Medicine and Hygiene, 38, 223–230.
Escobar, J., Gonzáles, R., Quiñones, M.L., Wilkerson, R.C. & Harrison, B. Presence of Anopheles (Kerteszia) pholidotus in a malaria focus in Colombia. pp. 315–316, in Clark G.G, & Rubio-Palis Y. (2010) Mosquito vector biology and control in Latin America – a 20th symposium. Journal of the American Mosquito Control Association, 26, 306–320.
Faran, M.E. (1980) Mosquito studies (Diptera, Culicidae) XXXIV. A revision of the Albimanus Section of the subgenus Nyssorhynchus of Anopheles. Contributions of the American Entomological Institute (Ann Arbor), 15, 1–215.
Forattini, O.P. (1962) Entomologia Médica. Parte geral, Diptera, Anophelinae Vol. 1. São Paulo, Brazil. 662 pp.
Forattini, O.P. (2002) Culicidologia Médica. Identificação, biologia, epidemiologia Vol. 2. São Paulo, Brazil. 864 pp.
González, R. & Carrejo, N. (2009) Introdución al estudio taxonómico de Anopheles de Colombia, claves y notas de distribución. Programa Editorial Universidad del Valle, Cali, Colombia. 260 pp.
Harbach, R.E. & Kitching, I.J. (1998) Phylogeny and classification of the Culicidae (Diptera). Systematic Entomology, 23, 327–370.
Harbach, R.E. & Knight, K.L. (1980) Taxonomists’ glossary of mosquito anatomy. Plexus Publishing, Inc., Marlton, N.J., U.S.A. 415 pp.
Harbach, R.E. & Knight, K.L. (1982) Corrections and additions to Taxonomists’ glossary of mosquito anatomy. Mosquito Systematics, 13, 201–217.
Harbach, R.E. & Navarro, J.C. (1996) A new species of Anopheles subgenus Kerteszia (Diptera: Culicidae) from Venezuela. Entomologica Scandinavica, 27, 207–216.
Hayes, J., Calderón, G., Falcon, R. & Zambrano, V. (1987) New incriminated anopheline vectors of human malaria parasites in Junín Department, Peru. Journal of the American Mosquito Control Association, 3, 418–422.
Hebert, P.D., Cywinska, A., Ball, S.L. & deWaard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London, Series B, 270, 313–321.
Katoh, K., Misawa, K., Kuma, K. & Miyata, T. (2002) MAFFT: A novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research, 30, 3059–3066.
Kimura, M. (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 6, 111–120.
Knight, K.L. & Stone, A. (1977) A catalog of the mosquitoes of the world (Diptera: Culicidae). 2nd edition. Thomas Say Foundation, Entomological Society of America, 6, xi+611 pp.
Komp, W.H.W. (1937) The species of the subgenus Kerteszia of Anopheles. Annals of the Entomological Society of America, 30, 492–529.
Komp, W.H.W. (1956) Notes on mosquitoes from an area of endemic Yellow fever in Colombia. Proceedings of the Entomological Society of Washington, 58, 37–42.
Komp, W.H.W. & Orsono-M., E. (1936) The male and larva of Anopheles (Kerteszia) boliviensis Theobald. Annals of the Entomological Society of America, 29, 415–419.
Kumar, S., Dudley, J., Nei, M. & Tamura, K. (2008) MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Briefings in Bioinformatics, 9, 299–306.
Lane, J. (1953) Neotropical Culicidae. Vol. 1. São Paulo, Brazil. 548 pp.
Li, C. & Wilkerson, R.C. (2005) Identification of Anopheles (Nyssorhynchus) albitarsis complex species (Diptera: Culicidae) using rDNA ITS2-based PCR primers. Memorias do Institute Oswaldo Cruz, 100, 495–500.
Linthicum, K.J. (1988) A revision of the Argyritarsis Section of the subgenus Nyssorhynchus of Anopheles (Diptera: Culicidae). Mosquito Systematics, 20, 98–271.
Linton, Y.-M., Harbach, R.E., Chang, M.S., Anthony, T.G. & Matusop, A. (2001) Morphological and molecular identity of Anopheles (Cellia) sundaicus (Diptera: Culicidae), the nominotypical member of a malaria vector species complex in Southeast Asia. Systematic Entomology, 26, 357–366.
Maddison, D.R. & Maddison, W.R. (2003) MacClade v.4.06: Analysis of phylogeny and character evolution. Sinauer Associates, Sunderland, MA.
Malafronte, R.D.S., Marrelli, M.T., Ramirez, C.L., Nassar, M.N. & Marinotti, O. (2007) Intraspecific variation of second internal transcribed spacer of nuclear ribosomal DNA among populations of Anopheles (Kerteszia) cruzii (Diptera: Culicidae). Journal of Medical Entomology, 44, 538–542.
Navarro, J.-C., Ventura, F.D., Zorrilla, A. & Liria, J. (2010) Registros de mayor altitud para mosquitos (Diptera: Culicidae) en Venezuela. Revista de Biologia Tropical (International Journal of Tropical Biology. ISSN-0034-7744) 58, 245–254.
Peyton, E.L., Wilkerson, R.C. & Harbach, R.E. (1992) Comparative analysis of the subgenera Kerteszia and Nyssorhynchus of Anopheles (Diptera: Culicidae). Mosquito Systematics, 24, 51–69.
Quiñones, M.L., Suárez, M.F., Rodriguez, A., Fleming, G.A. & Galvis, L.E. (1984) Comportamiento de Anopheles (Kerteszia) lepidotus Zavortink, 1973, y su incriminación como posible vector de malaria en el Departamento del Tolima, Colombia. Biomedica, 4, 5–13.
Ramirez, C.C.L. & Dessen, B.E.M. (2000a) Chromosomal evidence for sibling species of the malaria vector Anopheles cruzii. Genome, 43, 143–151.
Ramirez, C.C.L. & Dessen, B.E.M. (2000b) Chromosome differentiated populations of Anopheles cruzii: evidence for a third sibling species. Genetica, 108, 73–80.
Reinert, J.F. (1990) Medical Entomology Studies - XVII. Biosystematics of Kenknightia, a new subgenus of the mosquito genus Aedes Meigen from the Oriental Region (Diptera: Culicidae). Contributions of the American Entomological Institute (Gainesville), 26(2), 1–119.
Reinert, J.F. (2009) List of abbreviations for currently valid generic-level taxa in family Culicidae (Diptera). European Mosquito Bulletin, 27, 68–76.
Reinert, J.F., Harbach, R.E. & Kitching, I.J. (2008) Phylogeny and classification of Ochlerotatus and allied taxa (Diptera: Culicidae: Aedini) based on morphological data from all life stages. Zoological Journal of the Linnean Society, 153, 29-114.
Rona, L.D.P., Carvalho-Pinto, C.J., Gentile, C., Grisard, E.C. &Peixoto, A.A. (2009) Assessing the molecular divergence between Anopheles (Kerteszia) cruzii populations from Brazil using the timeless gene: Further evidence of a species complex. Malaria Journal, 8, 60.
Rona, L.D.P., Carvalho-Pinto, C.J. & Peixoto, A.A. (2010) Molecular evidence for the occurrence of a new sibling species within the Anopheles (Kerteszia) cruzii complex in South-East Brazil. Malaria Journal, 9, 33.
Ruiz, F., Linton, Y.-M., Ponsonby, D.J., Conn, J.E., Herrera, M., Quiñones, M.L. & Wilkerson R.C. (2010) Molecular comparison of topotypic specimens confirms Anopheles (Nyssorhynchus) dunhami Causey (Diptera: Culicidae) in the Colombian Amazon. Memories do Institute Oswaldo Cruz, 105, 899–903.
Saitou, N. & Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406–425.
Sallum, M.A.M., Dos Santos, C.L.S. & Wilkerson, R.C. (2009) Studies on Anopheles (Kerteszia) homunculus Komp (Diptera: Culicidae). Zootaxa, 2299, 1–18.
Sallum, M.A.M., Forattini O.P. & Wilkerson R.C. (2000) Redescription of the adult and larva and first description of the pupa of Anopheles (Kerteszia) laneanus. Journal of the American Mosquito Control Association, 16, 86–92.
Sallum, M.A.M., Schultz, T.R., Foster, P.G., Aronstein, K., Wirtz, R.A. & Wilkerson, R.C. (2002) Phylogeny of Anophelinae (Diptera: Culicidae) based on nuclear ribosomal and mitochondrial DNA sequences. Systematic Entomology, 27, 361–382.
Stone, A., Knight, K.L. & Starcke, H. (1959) A synoptic catalog of the mosquitoes of the world (Diptera, Culicidae). Thomas Say Foundation, Entomological Society of America, 6, 358 pp.
Sulzer, A.J., Cantella, R., Colichon, A., Gleason, N.N. & Walls, K.W. (1975) A focus of hyperendemic Plasmodium malariae – P. vivax with no P. falciparum in a primitive population in the Peruvian Amazon jungle. Bulletin of the World Health Organization, 52, 273–278.
Sulzer, A.J., Sulzer, K.R., Cantella, R., Colichon, H., Latorre, C.R. & Welch, M. (1978) Study of coinciding foci of malaria and leptospirosis in the Peruvian Amazon area. Transactions of the Royal Society of Tropical Medicine and Hygiene, 72, 76–83.
Swofford, D.L. (2003) PAUP*: phylogenetic analysis using parsimony, version 4.0b 10. Sinauer Associates, Sunderland, MA.
Tan, C.H., Vythilingam, V., Matusop, A., Chan, S.T. & Singh, B. (2008) Bionomics of Anopheles latens in Kapit, Sarawak, Malaysian Borneo in relation to the transmission of zoonotic simian malaria parasite Plasmodium knowlesi. Malaria Journal, 7, 52.
Tanaka, K., Mizusawa, K. & Saugstad, E.S. (1979) A revision of the adult and larval mosquitoes of Japan (including the Ryukyu Archipelago and the Ogasawara Islands) and Korea (Diptera: Culicidae). Contributions of the American Entomological Institute (Ann Arbor), 16, i–vii, 1–987.
Theobald, F.V. (1905) A catalogue of the Culicidae in the Hungarian National Museum with descriptions of new genera and species. Annales Historico-Naturales Musei Nationalis Hungarici, 3, 61–120.
Vythilingam, I., Yusuf, M.N.A., Tan, C.H., Adela, I.J., Yusof, M.Y., Abdul, H.A., Ismail, N.P., Abdullah, N.R. & Sulaiman, L.H. (2008) Plasmodium knowlesi in humans, macaques and mosquitoes in peninsular Malaysia. Parasites and Vectors, 1, 26.
Wilkerson, R.C. & Peyton, E.L. (1990) Standardized nomenclature for the costal wing spots of the genus Anopheles and the other spotted-wing mosquitoes (Diptera: Culicidae). Journal of Medical Entomology, 27, 207–224.
Wilkerson, R.C. & Peyton, E.L. (1991) The Brazilian malaria vector Anopheles (Kerteszia) cruzii: life stages and biology (Diptera: Culicidae). Mosquito Systematics, 23, 110–122.
Wood, D.M., Dang, P.T. & Ellis, R.A. (1979) The insects and arachnids of Canada part 6. The mosquitoes of Canada, Diptera: Culicidae. Biosystematics Research Institute, Canada Department of Agriculture Publication, 1686, 390 pp.
Zavortink, T.J. (1973) Mosquito Studies (Diptera, Culicidae) XXIX. A review of the subgenus Kerteszia of Anopheles. Contributions of the American Entomological Institute (Ann Arbor), 9, 1–54.