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Type: Articles
Published: 2012-02-10
Page range: 1–28
Abstract views: 38
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Discovery of the second European Amalopis species: an integrative survey of the widespread Pedicia (Amalopis) occulta (Meigen, 1830) (Insecta, Diptera, Pediciidae)

Department of Taxonomy and Ecology, Faculty of Biology and Geology, Babeş-Bolyai University, Clinicilor 5-7, 400006 Cluj, Romania
Biodiversität und Klima Forschungszentrum (BiK-F), Senckenberganlage 25, D-60325 Frankfurt am Main, Germany Molecular Biology Center, Babeş-Bolyai University, Treboniu Laurian 42, 400271 Cluj, Romania
Diptera Crane flies alpha taxonomy lectotype designation new species genetic differentiation DNA barcoding

Abstract

Integrative taxonomy enhances species discovery and facilitates species delimitation by combining DNA sequence data,morphology, and distributional and ecological information. In this paper we use complementary methods of morphologyand DNA barcoding to delineate species boundaries in a widespread European spring-dwelling crane-fly, Pedicia (Amalo-pis) occulta (Meigen). We describe a previously overlooked large cryptic dipteran as Pedicia (Amalopis) fusca n. sp. Wealso designate the lectotype of P. occulta of the basis of a comprehensive study of relevant type specimens. Morphologicaldifferences between the two species are delicate but detectable, and comprise mostly male genital structures. However,the sequence divergence of 13.1% reflects an ancient divergence, which plausibly pre-dates the Pleistocene. The EuropeanAmalopis species differ significantly from all the rest of Amalopis species with Far East distributions, but share a numberof similarities with a species identified from the Himalayas, India and described as Pedicia (Tricyphona) ericarum Alexander, 1966. We also discuss a possible close relationship between P. ericarum and the European Amalopis species.

References

  1. Alexander, C.P. (1913) Report on a collection of Japanese crane-flies (Tipulidae), with a key to the species of Ptychoptera [part]. Canadian Entomologist, 45, 313–322.

    Alexander, C.P. (1924) New or little-known Tipulidae (Diptera). XXVI. Palaearctic species. Annals and Magazine of Natural History (9), 15, 65–81.

    Alexander, C.P. (1927) The interpretation of the radial field of the wing in the nematocerous Diptera, with special reference to the Tipulidae. Proceedings of the Linnaean Society of New South Wales, 52, 42–72.

    Alexander, C.P. (1931) New or little-known Tipulidae from eastern Asia (Diptera). IX. Philippine Journal of Science, 44, 339–368.

    Alexander, C.P. (1945) Undescribed species of crane-flies from northern Korea (Diptera, Tipuloidea). Transactions of the Royal Entomological Society of London, 95, 227–246.

    Alexander, C.P. (1953) Records and descriptions of Japanese Tipulidae (Diptera). Part I. The crane-flies of Shikoku. I. Philippine Journal of Science, 82, 21–75.

    Alexander, C.P. (1957) Records and descriptions of Japanese Tipulidae (Diptera). Part V. The crane-flies of Honshu. I. Philippine Journal of Science, 85, 349–388.

    Alexander, C.P. (1958) Records and descriptions of Japanese Tipulidae (Diptera). Part VI. The crane-flies of Honshu. II. Philippine Journal of Science, 86, 281–330.

    Alexander, C.P. (1964) New or little-known species of Asiatic Tipulidae (Diptera). I. Transactions of the American Entomological Society, 90, 205–234.

    Alexander, C.P. (1966) New or little-known species of exotic Tipulidae (Diptera). XIII. Proceedings of the Royal Entomological Society of London (B), 35, 65–71.

    Andreanszky, G. (1968) Reste d'un lilas du Sarmatien Hongrois. Acta Botanica Academiae Scientiarium Hungaricae, 14, 133–145.

    Bálint, M., Botoşaneanu, L., Ujvárosi, L. & Popescu, O. (2009) Taxonomic revision of Rhyacophila aquitanica (Trichoptera: Rhyacophilidae), based on molecular and morphological evidence and change of taxon status of Rhyacophila aquitanica ssp. carpathica to Rhyacophila carpathica stat. n. Zootaxa, 2148, 39–48.

    Bálint, M., Domisch, S., Engelhardt, C.H.M., Haase, P., Lehrian, S, Sauer, J., Theissinger, K, Pauls, S.U. & Nowak, C. (2011) Cryptic biodiversity loss linked to global climate change. Nature Climate Change, 1, 313–318.

    Brindle, A. (1963) The natural groups of the British Pediciini (Dipt., Tipulidae). Entomologists Monthly Magazine, 98, 234–237.

    Brower, A.V. (1994) Rapid morphological radiation and convergence among races of the butterfly Heliconius erato inferred from patterns of mitochondrial DNA evolution. Proceedings of the National Academy of Sciences of the United States of America, 91, 6491–6495.

    Dienske, J.W. (1987) An illustrated key to the genera and subgenera of the western palaearctic Limoniidae (Insecta, Diptera) including a description of the external morphology. Stuttgarter Beitrage zur Naturkunde A, 409, 1–52.

    Ebach, M.C. (2011) Taxonomy and the DNA Barcoding Enterprise. Zootaxa, 2742, 67–68.

    Edwards, F.W. (1938) British short-palped craneflies. Taxonomy of adults. Transactions of the Society for British Entomology, 5, 1–168.

    Egger, J. (1863) Dipterologische Beitrage. Verhandlungen der Kaiserlich-Königlichen Zoologisch-Botanischen Gesellschaft in Wien, 13, 1101–1110.

    Förster, J.A. (1974) On the life and influence of J. W. Meigen. Mosquito Systematics, 6, 79–88.

    Gouy, M., Guindon, S. & Gascuel, O. (2010) Seaview version 4: A multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Molecular Biology and Evolution, 27, 221–224.

    Haliday, A.H. (1856) Addenda and Corrigenda. In: Walker, F., Insecta Brittanica, Diptera, III. London, xi–xxv.

    Hebert, P.D.N., Cywinska, A., Ball, S.L. & Waard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society London, 270, 313–321.

    ICZN (1999) International Code of Zoological Nomenclature. Fourth Edition. The International Trust for Zoological Nomenclature; c/o The Natural History Museum, London, xxix+360 pp.

    Kim, K-J. & Jansen, R.K. (1998) A chloroplast DNA phylogeny of lilacs (Syringa, Oleacea): plastone groups show a strong correlation with crossing groups. American Journal of Botany, 85, 1338–1351.

    Kimura, M. (1980) A simple method for estimating the evolutionary rate of base substitution through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120.

    Kolenati, F.A. (1859) Naturhistorische Durchforschung des Altvatergebirges. Jahresheft des Naturwissenschaftlischen Section der Māhrisch-Schelsischen Gesellschaft für Ackerbau unk Naturkunde, 1959, 1–83.

    Lackschewitz, P. (1940) Die palaarktischen Limnophilinen, Anisomerinen und Pediciinen des Wiener Naturhistorischen Museums. Annalen des Naturhistorischen Museums Wien, 50, 68–122.

    Latreille, P.A. (1809) Genera crustaceorum et insectorum secundum ordinem naturalem in familias dispositas, iconibus exemplisque plumiris explicate. Paris and Strasbourg, 4, 1–399.

    Lehrian, S., Bálint, M., Haase, P. & Pauls, S.U. (2010) Genetic population structure of an autumn emerging caddisfly with inherently low dispersal capacity and insights into its phylogeography. Journal of the North American Benthological Society, 29, 1100–1118.

    Macías-Hernández, N., Oromí, P. & Arnedo, M.A. (2010) Integrative taxonomy uncovers hidden species diversity in woodlouse hunter spiders (Araneae, Dysderidae) endemic to the Macaronesian archipelagos. Systematics and Biodiversity, 8, 531–553.

    Meigen, J.W. (1830) Systematische Beschreibung der bekannten europaischen zweiflugeligen Insekten. Friedrich Wilhelm Forstmann, Aachen, Germany, xi + 401 pp.

    Oosterbroek, P. (2011) Catalogue of the craneflies of the World (Insecta, Diptera, Nematocera, Tipuloidea). Available from http://ip30.eti.uva.nl/ccw/ (accessed on 8 April 2011).

    Oosterbroek, P., Dufour, C. & Pilipenko, V (2001) On the presence of Dolichopeza (subgenus Oropeza) in the westpalaearctic (Diptera, Tipulidae). Bulletin de la Societe Neuchateloise des Sciences Naturelles, 124, 119–123.

    Oosterbroek, P. & Theowald, B. (1991) Phylogeny of the Tipuloidea based on characters of larvae and pupae (Diptera, Nematocera) with an index to the literature except Tipulidae. Tijdschrift voor Entomologie, 134, 211–267.

    Padial, J., Miralles, A., la Riva, I.D. & Vences, M. (2010) The integrative future of taxonomy. Frontiers in Zoology, 7, 16.

    Papadopoulou, A., Anastasiou, I. & Vogler, A.P. (2010) Revisiting the Insect Mitochondrial Molecular Clock: The Mid-Aegean Trench Calibration. Molecular Biology and Evolution, 27 (7), 1659–1672.

    Pauls, S.U., Lumbsch, H.T. & Haase, P. (2006) Phylogeography of the montane caddisfly Drusus discolor: evidence for multiple refugia and periglacial survival. Molecular Ecology, 15, 2153–2169.

    Petersen, M.J., Bertone, M.A., Wiegmann, B.M. & Courtney, G.W. (2010) Phylogenetic synthesis of morphological and molecular data reveals new insights into the higher-level classification of Tipuloidea (Diptera). Systematic Entomology, 35, 526–545.

    Pierre, C. (1924) Dipteres: Tipulidae. Faune de France, 8, 1–159.

    Pont, A.C. (1995) The type-material of Diptera (Insecta) described by G.H. Verrall and J.E. Collin, Clarendon press, Oxford: i–x, 1–223.

    Previšić, A., Walton, C., Kucinić, M., Mitrikeski, P.T. & Kerovec, M. (2009) Pleistocene divergence of dinaric Drusus endemics (Trichoptera, Limnephilidae) in multiple microrefugia within the Balkan Peninsula. Molecular Ecology, 18, 634–47.

    Ribeiro, G.C. (2008) Phylogeny of the Limnophilinae (Limoniidae) and early evolution of the Tipulomorpha (Diptera). Invertebrate Systematics, 22, 627–694.

    Savchenko, E.N. (1986) Komary-limoniidy [Limoniid-flies]. (General description, subfamilies Pediciinae and Hexatominae). Fauna Ukrainy, 14, 1–380.

    Savchenko, E.N. & Krivolutskaya, G.O. (1976): Limoniidae of the south Kuril Islands and south Sakhalin. Akademiya Nauk Ukrainskai SSR, Kiev, 1–160.

    Schlick-Steiner, B.C., Steiner, F.M., Seifert, B., Stauffer, C., Christian, E. & Crozier, R.H. (2010) Integrative taxonomy: a multisource approach to exploring biodiversity. Annual Review of Entomology, 55, 421–438.

    Sotiropoulos, K., Eleftherakos, K., Dzukic, G., Kalezic, M., Legakis, A. & Polymeni, R. (2007) Phylogeny and biogeography of the alpine newt Mesotriton alpestris (Salamandridae, Caudata), inferred from mtDNA sequences. Molecular Phylogenetics and Evolution, 45, 211–226.

    Ståhls, G. & Savolainen, E. (2008) MtDNA COI barcodes reveal cryptic diversity in the Baetis vernus group (Ephemeroptera, Baetidae). Molecular Phylogenetics and Evolution, 46, 82–87.

    Starý, J. (1992) Phylogeny and classification of Tipulomorpha, with special emphasis on the family Limoniidae. Acta Zoologica Cracoviensia, 35, 11–36.

    Starý, J. (2006) Nomenclatural changes in West Palaearctic Limoniidae and Pediciidae (Diptera). Acta Universitatis Carolinae, Biologica, 49, 175–186

    Ujvárosi, L., Bálint, M., Schmitt, T., Mészáros, N., Ujvárosi, T. & Popescu, O. (2010a) Divergence and speciation in the Carpathians area: patterns of morphological and genetic diversity of the crane fly Pedicia occulta (Diptera: Pediciidae). Journal of the North American Benthological Society, 29, 1075–1088.

    Ujvárosi, L., Bálint, M., Kolcsár, L., Mihali, C. (2010b) Pediciidae larvae (Insecta: Diptera) in the Carpathian Basin: Preliminary results and further perspectives. Acta Biologica Debrecina, Supplimenta Oecologica Hungarica, 21, 233–246.

    Verrall, G.H. (1888) List of British Tipulidae, &c. (Daddy-Longlegs), with notes. Entomologists Monthly Magazine, 25, 117–125.

    Zetterstedt, J. W. (1837) Conspectus familiarum, generum et specierum Dipterorum, in fauna insectorum Lapponica descriporum. Isis (Okens), 1, 28–67.

    Zhou, X., Adamowicz, S., Jacobus, L., DeWalt, R.E. & Hebert, P. (2009) Towards a comprehensive barcode library for arctic life – Ephemeroptera, Plecoptera, and Trichoptera of Churchill, Manitoba, Canada. Frontiers in Zoology, 6, 30.