Skip to main content Skip to main navigation menu Skip to site footer
Type: Articles
Published: 2011-11-29
Page range: 49–58
Abstract views: 53
PDF downloaded: 5

Repeatability and precision in proboscis length measurements for long proboscid flies

Department of Entomology, North Carolina State University, Raleigh, NC, 27695-7613
Diptera Proboscis long-tongued fly Tabanidae Nemestrinidae measurement morphology co-divergence pollination ecology

Abstract

Methods for measuring proboscides in long-tongued fly pollinators are examined with respect to fly morphology and behavior. Most ecological studies aim to measure the functional proboscis length as a response or predictor variable. Here I suggest a proxy for this, the Maximum Functional Length (MFL), obtained by combining the lengths of the prementum and labrum. I also quantify errors in proboscis measurements due to a lack of consideration of the extensible ventral rostral membrane (VRM). Results show that naïve measurements allow for substantial unrepeatable variation (mean: 7mm; maximum 14mm).

References

  1. Alexandersson, R. & Johnson, S.D. (2002) Pollinator-mediated selection on flower-tube length in a hawkmoth-pollinated Gladiolus (Iridaceae). Proceedings of the Royal Society of London, Series B: Biological Sciences, 269, 631–636.

    Anderson, B. & Johnson, S. (2006) The effects of floral mimics and models on each others' fitness. Proceedings of the Royal Society of London, Series B: Biological Sciences, 273, 969–974.

    Anderson, B., Johnson, S. & Carbutt, C. (2005) Exploitation of a specialized mutualism by a deceptive orchid. American Journal of Botany, 92, 1342–1349.

    Anderson, B. & Johnson, S.D. (2008) The geographical mosaic of coevolution in a plant-pollinator mutualism. Evolution, 62, 220–225.

    Archibald, J., Wolfe, A. & Johnson, S. (2004) Hybridization and gene flow between a day-and night-flowering species of Zaluzianskya (Scrophulariaceae SS, tribe Manuleeae). American Journal of Botany, 91, 1333–1344.

    Bertoloni, G. (1861) Illustrazione dei prodotti naturali del Mozambico. [Exhibit and description of new Ditpera from Mozambique.]. Memorie della Accademia delle scienze dell'Istituto di Bologna. Classe di scienze fisiche, 1860–1861, 28–29.

    Bigot, J.M.F. (1892) Descriptions de Dipteres nouveaux. Tabanidi. Mémoires de la Société Zoologique de France, 5, 602–691.

    Bloch, D. & Erhardt, A. (2008) Selection toward shorter flowers by butterflies whose probosces are shorter than floral tubes. Ecology, 89, 2453–2460.

    Bonhag, P.F. (1951) The skeleto-muscular mechanism of the head and abdomen of the adult horsefly (Diptera: Tabanidae). Transactions of the American Entomological Society, 78, 131–202.

    Borrell, B.J. & Krenn, H.w. (2006) Nectar Feeding in Long-Proboscid Insects. Ecology and biomechanics: a mechanical approach to the ecology of animals, 9, 185–212.

    Campbell, D.R., Waser, N.M. & Price, M.V. (1994) Indirect selection of stigma position in Ipomopsis aggregata via a genetically correlated trait. Evolution, 48, 55–68.

    Campbell, D.R., Waser, N.M. & Price, M.V. (1996) Mechanisms of hummingbird-mediated selection for flower width in Ipomopsis aggregata. Ecology (Washington D C), 77, 1463–1472.

    Carroll, S.P. & Boyd, C. (1992) Host Race Radiation in the Soapberry Bug - Natural-History with the History. Evolution, 46, 1052–1069.

    Carroll, S.P., Dingle, H., Famula, T.R. & Fox, C.W. (2001) Genetic architecture of adaptive differentiation in evolving host races of the soapberry bug, Jadera haematoloma. Genetica, 112, 257–272.

    Combs, J.K. & Pauw, A. (2009) Preliminary evidence that the long-proboscid fly, Philoliche gulosa, pollinates Disa karooica and its proposed Batesian model Pelargonium stipulaceum. South African Journal of Botany, 75, 757–761.

    Cumming, J.M. & Wood, M.D. (2009) Adult morphology and terminology. In: B. V. Brown, A. Borkent, J. M. Cumming, M. D. Wood, N. E. Woodley & M. A. Zambudo (Eds), Manual of Central American Diptera. Vol. 1. NRC Research Press, Ottawa.

    Downes, J.A. (1971) The ecology of blood-sucking Diptera: an evolutionary perspective. In: A. M. Fallis (Ed), Ecology and physiology of parasites. University of Toronto Press, Toronto, Canada, pp. 232–258.

    Downes, J.A. & Colless, D. (1967) Mouthparts of the biting and blood-sucking type in Tanyderidae and Chironomidae (Diptera). Nature, 214, 1355–1356.

    Goldblatt, P., Bernhardt, P. & Manning, J.C. (2000) Adaptive radiation of pollination mechanisms in Ixia (Iridaceae: Crocoideae). Annals of the Missouri Botanical Garden, 87, 564–577.

    Goldblatt, P. & Manning, J.C. (1999) The long-proboscid fly pollination system in Gladiolus (Iridaceae: Ixoideae). Annals of the Missouri Botanical Garden, 86, 758–774.

    Goldblatt, P. & Manning, J.C. (2000) The long-proboscid fly pollination system in southern Africa. Annals of the Missouri Botanical Garden, 87, 146–170.

    Goldblatt, P. & Manning, J.C. (2006) Radiation of pollination systems in the Iridaceae of sub-Saharan Africa. Annals of botany, 97, 317.

    Grimaldi, D. (1988) Bee flies and bluets: Bombylius (Diptera: Bombyliidae) flower-constant on the distylous species, Hedyotis caerulea (Rubiaceae), and the manner of foraging. Journal of Natural History, 22, 1–10.

    Jobling, B. (1976) On the fascicle of blood-sucking Diptera. In addition a description of the maxillary glands in Phlebotomus papatasi, together with the musculature of the labium and pulsatory organ of both the latter species and also of some other Diptera. Journal of Natural History. London., 10, 457–461.

    Johnson, S.D. (1995) Observations of hawkmoth pollination in the South African orchid Disa cooperi. Nordic Journal of Botany, 15, 121–125.

    Johnson, S.D. (2000) Batesian mimicry in the non-rewarding orchid Disa pulchra, and its consequences for pollinator behaviour. Biological Journal of the Linnean Society, 71, 119–132.

    Johnson, S.D., Edwards, J., Carbuttt, C. & Potgieter, C.J. (2002) Specialization for hawkmoth and long-proboscid fly pollination in Zaluzianskya section Nycterinia (Scrophulariaceae). Botanical Journal of the Linnean Society, 138, 17–27.

    Johnson, S.D. & Midgley, J. (1997) Fly pollination Gorteria diffusa (Asteraceae), and a possible mimetic funtion for dark spots on the capitulum. American Journal of Botany, 84, 429–436.

    Johnson, S.D. & Morita, S. (2006) Lying to Pinocchio: floral deception in an orchid pollinated by long-proboscid flies. Botanical Journal of the Linnean Society, 152, 271–278.

    Johnson, S.D. & Steiner, K.E. (1995) Long-proboscid fly pollination of two orchids in the Cape Drakensberg mountains, South Africa. Plant Systematics and Evolution, 195, 169–175.

    Johnson, S.D. & Steiner, K.E. (1997) Long-tongued fly pollination and evolution of floral spur length in the Disa draconis complex (Orchidaceae). Evolution, 51, 45–53.

    Kearns, C.A. & Inouye, D.W. (1993): Techniques for pollination biologists. University Press of Colorado, Niwot, Colorado.

    Krenn, H. (1998) Proboscis sensilla in Vanessa cardui (Nymphalidae, Lepidoptera): functional morphology and significance in flower-probing. Zoomorphology, 118, 23–30.

    Lichtwardt, B. (1910) Beiträg zur kenntnis der Nemestriniden (Dipt.). Deutsche Entomologische Zeitschrift, Teil V., 371–388; 589–624.

    Linnaeus, C. (1764) Museum S'ae R'ae M'tis Ludovicae Ulriciae Reginae, Svecorum, Gothorum, Vandalorumque, &c. In: Quo Animalia Rariora, Exotica, Imprimis, Insecta, & Conchilia Describuntur et Determinantur. Salvius, Holmiae [= Stockholm], p. vi + 720 pp.

    Manning, J.C. & Goldblatt, P. (1996) The Prosoeca peringueyi (Diptera: Nemestrinidae) pollination guild in southern Africa: Long-tongued flies and their tubular flowers. Annals of the Missouri Botanical Garden, 83, 67–86.

    Manning, J.C. & Goldblatt, P. (1997) The Moegistorhynchus longirostris (Diptera: Nemestrinidae) pollination guild: Long-tubed flowers and a specialized long-proboscid fly pollination system in southern Africa. Plant Systematics and Evolution, 206, 51–69.

    Manning, J.C. & Goldblatt, P. (2005) Radiation of pollination systems in the Cape genus Tritoniopsis (Iridaceae : Crocoideae) and the development of bimodal pollination strategies. International Journal of Plant Sciences, 166, 459–474.

    Molleman, F., Krenn, H.W., Alphen, M.E.V., Brakefield, P.M., Devries, P.J. & Zwaan, B.J. (2005) Food intake of fruit-feeding butterflies: evidence for adaptive variation in proboscis morphology. Biological Journal of the Linnean Society, 86, 333–343.

    Morita, S. (2008) A revision of the Philoliche aethiopica species complex (Diptera: Tabanidae). African Invertebrates, 49, 129–158.

    Muchhala, N. (2006) Nectar bat stows huge tongue in its rib cage. Nature, 444, 701–702.

    Muchhala, N. (2007) Adaptive trade-off in floral morphology mediates specialization for flowers pollinated by bats and hummingbirds. The American Naturalist, 169, 494–504.

    Pauw, A., Stofberg, J. & Waterman, R.J. (2009) Flies and flowers in Darwin's race. Evolution, 63, 268–279.

    Potgieter, C., Edwards, T.J. & Van Staden, J. (2007) Pollination of southern African species of Plectranthus (Lamiaceae) by flies with moderate proboscid lengths (Acroceridae, Tabanidae and Nemestrinidae). South African Journal of Botany, 73, 306–307.

    Potgieter, C.J., Edwards, T.J., Miller, R.M. & Van Staden, J. (1999) Pollination of seven Plectranthus spp. (Lamiaceae) in southern Natal, South Africa. Plant Systematics and Evolution, 218, 99–112.

    Potgieter, C.J., Edwards, T.J. & Van Staden, J. (2009) Pollination of Plectranthus spp. (Lamiaceae) with sigmoid flowers in southern Africa. South African Journal of Botany, 75, 646–659.

    Schiner, I.R. (1867[1868]) Zweiter Bericht uber die von der Weltumseglungsreise der k. Fregatte Novara mitgebrachten Dipteren. Abhandlungen der [K.-k. (=Kaiserlich-königlichen)] Zoologisch-Botanischen Gesellschaft in Wien, 1867, 303–314.

    Stang, M., Klinkhammer, P.G.L. & van der Meijden, E. (2006) Size constraints on flower abundance determine the number of linteractions in a plant-flower visitor web. Oikos, 112, 111–121.

    Stuck, M. (1997) Floral divergence and convergence in the genus Pelargonium (Geraniaceae) in southern Africa: ecological and evolutionary considerations. Plant Systematics and Evolution, 208, 71–97.

    Szucsich, N. & Krenn, H. (2002) Flies and concealed nectar sources: morphological innovations in the proboscis of Bombyliidae (Diptera). Acta Zoologica, 83, 183–192.

    Szucsich, N. & Krenn, H.w. (2000) Morphology and function of the proboscis in Bombyliidae (Diptera, Brachycera) and implication for proboscis evolution in Brachycera. Zoomorphology, 120, 79–90.

    Thunberg, C. (1789) D.D. Museum Naturalium Academiae Upsaliensis. In: Cujus Partem Septiman [= Part 7], Upsaliae [=Uppsala], pp. [ii] + pp. 85–94.

    Toju, H. (2008) Fine scale local adaptation of weevil mouthpart length and camellia pericarp thickness: altitudinal gradient of a putative arms race. Evolution, 62, 1086–1102.

    Toju, H. & Sota, T. (2006) Phylogeography and the geographic cline in the armament of a seed-predatory weevil: effects of historical events vs. natural selection from the host plant. Molecular Ecology, 15, 4161–4173.

    Wiedemann, C.R.W. (1828) Aussereuropäische zweiflügelige Insekten. Erster Theil. [1st part]. (Vol. 1). Schulz, Hammburg.

    Wiegmann BM, Trautwein MD, Winkler IS, Barr NB, Kim J-W, Lambkin C, Bertone MA, Cassel BK, Bayless KM, Heimberg AM, Wheeler BM, Peterson KJ, Pape T Sinclair BJ, Skevington JH, Blagoderov V, Caravas J, Kutty SN, Schmidt-Ott U, Kampmeier G, Thompson FC, Grimaldi DA, Beckenbach AT, Courtney GW, Friedrich M, Meier R, & Yeates D. (2011) Episodic radiations in the fly tree of life. Proceedings of the National Academy of Sciences of the United States of America 108, 5690–5695