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Issue: Vol. 2933 No. 1: 29 Jun. 2011
Type: Articles
Published: 2011-06-29
DOI: 10.11646/zootaxa.2933.1.3
Page range: 46-54
Abstract views: 50
PDF downloaded: 3

Barcoding without DNA? Species identification using near infrared spectroscopy

Departamento de Zoologia, Universidade Federal do Paraná, UFPR, Curitiba, Paraná, 81531- 980, Brazil Instituto de Ecologia. La Paz, Bolivia
Departamento de Zoologia, Universidade Federal do Paraná, UFPR, Curitiba, Paraná, 81531- 980, Brazil
Instituto de Química, Universidade Estadual de Campinas, UNICAMP, Campinas, São Paulo, 13084-971, Brazil
Departamento de Química, Universidade Federal do Rio Grande do Norte, Lagoa Nova, Natal 59072-970, Brazil
Departamento de Zoologia, Universidade Federal do Paraná, UFPR, Curitiba, Paraná, 81531- 980, Brazil
Centro de nanociencias y nanotecnología, Universidad Nacional Autónoma de México, UNAM, Apartado postal 356. Ensenada, B. C. México
General metabolomics Neodexiopsis DNA barcoding near infrared spectroscopy

Abstract

Hennig's holomorphology concept defines taxonomy as a process that synthesizes evidence from all relevant comparative sources. One possible source is metabolomics, in which the global metabolic profile is analyzed. An integral metabolic profile can be quickly obtained, nondestructively, through spectroscopy in the near infrared region. Here we use near infrared spectroscopy and chemometry to identify nine species of flies in the genus Neodexiopsis Malloch (Muscidae, Diptera). This genus is the most species-rich of the Muscoidea in the Neotropical region. Identification success demonstrates that near infrared spectroscopy may provide a new source of data to test and organize hypotheses of species delimitation. Comparing near infrared spectroscopy with DNA barcoding, spectroscopy may have even greater conceptual merit as a true barcode of life.

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