Abstract
Recently, DNA has become a major source for phylogenetic inference. Although, in entomology, morphology-based methods of taxon identification and the use of morphological data for phylogenetic analyses remain prevalent, the value of these types of studies (often called “traditional”) are depreciated from time to time. It is obvious, that wrong taxon identification may affect results of any taxonomic study. Here we examine evidence that not only the modern “molecular” taxonomists, but even the so-called “traditional” taxonomists, may be casual and irresponsible in taxa identification, when they do their phylogenomic research. We also argue that a researcher’s responsibility for proper taxon identification and its naming is actually much higher in molecular studies than in any others.
References
Assis, L.C.S. & Rieppel, O. (2011) Are monophyly and synapomorphy the same or different? Revisiting the role of morphology in phylogenetics. Cladistics, 27, 94–102.
Bybee, S.M., Zaspel, J.M., Beucke, K.A., Scott, C.H., Smith, B.W. & Branham, M.A. (2010) Are molecular data supplanting morphological data in modern phylogenetic studies? Systematic Entomology, 35, 2–5.
DeSalle, B. (2006) Species discovery versus species identification in DNA barcoding efforts: response to Rubinoff. Conservation Biology, 20, 1545–1547.
Ebach, M.C. (2011) Taxonomy and the DNA barcoding enterprise. Zootaxa, 2742, 67–68.
Ebach, M.C. & Holdrege, C. (2005a) DNA barcoding is no substitute for taxonomy. Nature, 434, 697.
Ebach, M.C. & Holdrege, C. (2005b) More taxonomy, not DNA barcoding. Bioscience, 55, 822–823.
Frézal, L. & Leblois, R. (2008) Four years of DNA barcoding: Current advances and prospects. Infection, Genetics and Evolution, 8, 727–736.
Grazia, J., Schuh, R.T. & Wheeler, W.C. (2008) Phylogenetic relationships of family groups in Pentatomoidea based on morphology and DNA sequences (Insecta: Heteroptera). Cladistics, 24, 1–45.
Hajibabaei, M., Singer, G.A.C., Hebert, P.D.N. & Hickey, D.A. (2007) DNA barcoding: how it complements taxonomy, molecular phylogenetics and population genetics. Trends in Genetics, 23, 167–172.
Hebert, P.D.N. & Gregory, T.R. (2005) The promise of DNA barcoding for taxonomy. Systematic Biology, 54, 852–859.
Hołyński, R. (2010) Taxonomy and mediocrity of DNA barcoding – some remarks on Packer et al. 2009: DNA barcoding and the mediocrity of morphology. Arthropod Systematics & Phylogeny, 68, 143–150.
Hsiao, T.Y., Zheng, L.Y. & Ren, S.Z. (1977) A handbook for the determination of the Chinese Hemiptera-Heteroptera, vol. 1. 330 pp. Tientsin.
Hua, J., Li, M., Dong, P., Cui, Y., Xie, Q. & Bu, W. (2008) Comparative and phylogenomic studies on the mitochondrial genomes of Pentatomomorpha (Insecta: Hemiptera: Heteroptera). BMC Genomics, 9: DOI:10.1186/1471-2164-9-610.
Lee, W., Kang, J., Jung, Ch., Hoelmer, K., Lee, S.H. & Lee, S. (2009) Complete mitochondrial genome of brown marmorated stink bug Halyomorpha halys (Hemiptera: Pentatomidae), and phylogenetic relationships of hemipteran suborders. Molecules and Cells, 28, 155–l65.
Li, H.M. (2006) Advances in the study of systematics and phylogenetics of Pentatomomorpha insects. Journal of Zhongkai University of Agriculture and Technology, 19, 62–66.
Li, H.M., Deng, R.Q., Wang, J.W., Chen, Z.Y., Jia, F.L. & Wang, X.Z. (2005) A preliminary phylogeny of the Pentatomomorpha (Hemiptera: Heteroptera) based on nuclear 18S rDNA and mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 37, 313–326.
Li, H.M., Deng, R.Q. & Wang, X.Z. (2006a) Phylogenetic relationships of the Pentatomomorpha (Hemiptera: Heteroptera: inferred from nuclear 18S rDNA sequences. Zoological Records, 27, 307–316.
Li, H.-M., Wang, X.-Z. & Lin, J.-T. (2006b) Phylogenetic relationships of the Pentatomoidea based on the mitochondrial 16S rDNA sequences (Heteroptera: Pentatomomorpha). Journal of Huazhong Agricultural University, 25, 507–511.
Lis, J.A. (2000) A revision of the burrower-bug genus Macroscytus Fieber, 1860 (Hemiptera: Heteroptera: Cydnidae). International Journal of Invertebrate Taxonomy – Genus, 11, 359–509.
Lis, J.A. (2006) Family Thyreocoridae Amyot & Serville, 1843 – negro-bugs. Catalogue of the Heteroptera of the Palaearctic Region. Volume 5. Pentatomomorpha II (ed. by Aukema, B. and Ch. Rieger), pp. 148–149. Netherlands Entomological Society, Wageningen.
Lis, J.A. (2010) Coxal combs in the Cydnidae sensu lato and three other related “cydnoid” families – Parastrachiidae, Thaumastellidae, Thyreocoridae (Hemiptera: Heteroptera): functional, taxonomic, and phylogenetic significance. Zootaxa, 2476, 53–64.
Lis, J.A. & Ziaja, D.J. (2010) Pretarsal structures in the family Cydnidae sensu lato (Hemiptera: Heteroptera: Pentatomoidea). Zootaxa, 2545, 23–32.
Lis, J.A., Ziaja, D.J. & Lis, P. (2011) Recovery of mitochondrial DNA for systematic studies of Pentatomoidea (Hemiptera: Heteroptera): successful PCR on early 20th century dry museum specimens. Zootaxa, 2748, 18–28.
Liu, H., Li, Z., Li, S. & Cai, W. (2007) Application of mitochondrial DNA sequences in molecular systematics of true bugs (Hemiptera: Heteroptera). Entomotaxonomia, 29, 265–274.
Mitchell, A. (2011) DNA barcoding is useful for taxonomy: a reply to Ebach. Zootaxa, 2772, 67–68.
Packer, L., Gibbs, J., Sheffield, C. & Hanner, R. (2009) DNA barcoding and the mediocrity of morphology. Molecular Ecology Resources, 9 (Suppl.), 1, 42–50.
Pluot-Sigwalt, D. & Lis, J.A. (2008) Morphology of the spermatheca in the Cydnidae (Hemiptera: Heteroptera): bearing of its diversity on classification and phylogeny. European Journal of Entomology, 105, 279–312.
Scotland, R.W., Olm, R.G. & Bennett, J.R. (2003) Phylogeny reconstruction: the role of morphology. Systematic Biology, 52, 539–548.
Vogler, A.P. & Monaghan, M.T. (2007) Recent advances in DNA taxonomy. Journal of Zoological Systematics and Evolutionary Research, 45, 1–10.
Will, K.W. & Rubinoff, D. (2004) Myth of the molecule: DNA barcodes for species cannot replace morphology for identification and classification. Cladistics, 20, 47–55.
Will, K.W., Mishler, B.D. & Wheeler, Q.D. (2005) The perils of DNA barcoding and the need for integrative taxonomy. Systematic Biology, 54, 844–851.
Xie, Q., Bu, W. & Zheng, L. (2005) The Bayesian phylogenetic analysis of the 18S rRNA sequences from the main lineages of Trichophora (Insecta: Heteroptera: Pentatomomorpha). Molecular Phylogenetics and Evolution, 34, 448–451.
Zhu, G.P., Liu, G.Q. & Lis, J.A. (2010) A study on the genus Macroscytus Fieber, 1860 from China (Hemiptera: Heteroptera: Cydnidae). Zootaxa, 2400, 1–15.