Skip to main content Skip to main navigation menu Skip to site footer
Type: Articles
Published: 2011-02-02
Page range: 57–65
Abstract views: 66
PDF downloaded: 1

Phylogenetic relationships among the Epiperipatus lineages (Onychophora: Peripatidae) from the Minas Gerais State, Brazil

Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901, Belo Horizonte, MG, Brazil
Institute of Biology: Animal Evolution & Development, University of Leipzig, Talstr. 33, D-04103 Leipzig, Germany
Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901, Belo Horizonte, MG, Brazil
Onychophora Phylogeny phylogeography mtDNA velvet worms cryptic speciation

Abstract

The taxonomy and phylogeny of Onychophora (velvet worms), in particular of the neotropical Peripatidae, are understudied, due to a low number of useful morphological characters. In this paper, we use mitochondrial DNA markers to investigate the evolutionary relationships among the Epiperipatus lineages from the Minas Gerais State of Brazil. Our phylogenetic analyses of two fragments of the cytochrome oxidase subunit I (COI) and small ribosomal subunit RNA genes (12S rRNA) provide evidence of three major monophyletic clades and indicate cryptic speciation among specimens from different geographic localities. Our findings highlight the utility of molecular methods for studies of cryptic speciation, phylogeography, and evolutionary history of Onychophora.

References

  1. Bouvier, E.L. (1902) Peripatus biolleyi, Onychophore nouveau de Costa-Rica. Bulletin de la Société Entomologique de France, 16, 258–259.

    Bursell, E. & Ewer, D.W. (1950) On the reactions to humidity of Peripatopsis moseleyi.. Journal of Experimental Biology, 26, 335–353.

    Daniels, S.R. & Ruhberg, H. (2010) Molecular and morphological variation in a South African velvet worm Peripatopsis moseleyi (Onychophora, Peripatopsidae): evidence for cryptic speciation. Journal of Zoology, 282, 171–179.

    Daniels, S.R., Picker, M.D., Cowlin, R.M. & Hamer, M.L. (2009) Unravelling evolutionary lineages among South African velvet worms (Onychophora: Peripatopsidae) provides evidence for widespread cryptic speciation. Biological Journal of the Linnean Society, 97, 200–216.

    Ewing, B. & Green, P. (1998) Base-calling of automated sequencer traces using Phred. II. Error probabilities. Genome Research, 8, 186–194.

    Ewing, B., Hillier, L.D., Wendl, M.C. & Green, P. (1998) Base-Calling of automated sequencer traces using Phred. I. Accuracy assessment. Genome Research, 8, 175–185.

    Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39, 783–791.

    Gleeson, D.M., Rowell, D.M., Tait, N.N., Briscoe, D.A. & Higgins, A.V. (1998) Phylogenetic relationships among Onychophora from Australasia inferred from the mitochondrial cytochrome oxidase subunit I gene. Molecular Phylogenetics and Evolution, 10, 237–248.

    Gordon, D., Abajian, C. & Green, P. (1998) Consed: a graphical tool for sequence finishing. Genome Research, 8, 195–202.

    Green, P. (1994) Phrap. Available via http://www.genome.washington.edu/UWGC/ analysistools/phrap.htm.

    Guindon, S. & Gascuel, O. (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology, 52, 696–704.

    Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogeny. Bioinformatics, 17, 754–755.

    Kumar, S., Tamura, K. & Nei, M. (2007) MEGA4 (Molecular Evolutionary Genetics Analysis). Ver. 4.0. [Computer software]. Tempe: Arizona State University.

    Marcus, E. & Marcus, E. (1955) A new Peripatus from Minas Gerais, Brazil. Anais da Academia Brasileira de Ciências, 27, 189–193.

    Mishler, B.D. & Theriot, E.C. (2000) The phylogenetic species concept (sensu Mishler and Theriot): Monophyly, apomorphy, and phylogenetic species concepts. In: Wheeler, Q.D. & Meier, R. (eds.). Species Concepts and Phylogenetic Theory: A Debate. Columbia University Press, New York, 44–54.

    Monge-Nájera, J., Barrientos, Z. & Aguilar, F. (1993) Behavior of Epiperipatus biolleyi (Onychophora: Peripatidae) under laboratory conditions. Revista de Biología Tropical, 41, 689–696.

    Oliveira, I.S. & Wieloch, A.H. (2005) Macroperipatus machadoi sp. n. (Onychophora: Peripatidae) da Floresta Atlântica de Minas Gerais, Brasil. Lundiana, 6, 61–66.

    Oliveira, I.S., Wieloch, A.H. & Mayer, G. (2010) Revised taxonomy and redescription of two species of the Peripatidae (Onychophora) from Brazil: a step towards consistent terminology of morphological characters. Zootaxa, 2492, 16–34.

    Posada, D. & Crandall, A. (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics, 14, 817–818.

    Rockman, M.V., Rowell, D.M. & Tait, N.N. (2001) Phylogenetics of Planipapillus, lawn-headed onychophorans of the Australian Alps, based on nuclear and mitochondrial gene sequences. Molecular Phylogenetics and Evolution, 21, 103–116.

    Sambrook, J., Russel, D.W. & Sambrook, J. (2001) Molecular Cloning: A Laboratory Manual, CHSL Press, New York.

    Sampaio-Costa, C., Chagas-Junior, A. & Baptista, R.L.C. (2009) Brazilian species of Onychophora with notes on their taxonomy and distribution. Zoologia, 26, 553–561.

    Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Floor, P. (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America, 87, 651–701.

    Sunnucks, P. & Wilson, A.C.C. (1999) Microsatellite markers for the onychophoran Euperipatoides rowelli. Molecular Ecology, 8, 899–900.

    Swofford, D. (2002) PAUP*: Phylogenetic Analysis Using Parsimony (and other methods). Ver. 4. [Computer software and manual]. Sinauer Associates. Sunderland, Massachusetts.

    Trewick, S.A. (1998) Sympatric cryptic species in New Zealand Onychophora. Biological Journal of the Linnean Society, 63, 307–329.

    Trewick S.A. (1999) Molecular diversity of Dunedin peripatus (Onychophora: Peripatopsidae). New Zealand Journal of Zoology, 26, 381–393.

    Trewick, S.A. (2000) Mitochondrial DNA sequences support allozyme evidence for cryptic radiation of New Zealand Peripatoides (Onychophora). Molecular Ecology, 9, 269–28.

    Xia, X. & Xie, Z. (2001) DAMBE: Data analysis in molecular biology and evolution. Journal of Heredity, 92, 371–373.