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Issue: Vol. 2745 No. 1: 20 Jan. 2011
Type: Articles
Published: 2011-01-20
DOI: 10.11646/zootaxa.2745.1.3
Page range: 43–52
Abstract views: 63
PDF downloaded: 2

Morphometric differentiation of five biotypes of Lysiphlebus fabarum (Marshall) (Hymenoptera: Braconidae: Aphidiinae) in Iran

Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
Department of Ecology, International Center for Science, High Technology & Environmental Sciences, Kerman, Iran Current address: Institute of Ecology, Friedrich-Schiller-University Jena, Dornburger Str. 159, 07743 Jena, GERMANY. Permanent address: Department of Ecology, International Center for Science, High Technology & Environmental Sciences, Kerman, 7631133131, IRAN
Hymenoptera morphometrics Lysiphlebus host-associated variation aphid parasitoids

Abstract

Morphometric analyses were carried out on five biotypes of Lysiphlebus fabarum (Marshall) (Hymenoptera: Braconidae: Aphidiinae) associated with five host aphid species, Aphis craccivora Koch, Aphis fabae Scopoli, Aphis gossypii Glover, Brachyunguis zygophylli (Nevsky) and Melanaphis sacchari (Zehntner). Sixteen morphological characters were measured on female specimens of L. fabarum. Two multivariate discriminant analyses, discriminant function analysis and Unweighted Pair Group Method with Arithmetic mean cluster analysis, were employed to discriminate among L. fabarum biotypes on a morphological basis. Both discriminant function analysis and cluster analysis revealed significant morphological differences. Specimens of L. fabarum reared on Melanaphis sacchari/ Sorghum halepense association showed clear differentiation from other L. fabarum biotypes. A stepwise discriminant function analysis selected Distance between R1-R2 junction and outer margin of wing, Metacarpus length, Width of first tergit, Basal vein length and Femur length that showed the highest contribution to the separation of host-adapted biotypes. A dendrogram of the cluster analysis based on data of squared Euclidean distances classified biotypes in different groups. This study demonstrates that morphometric analysis of morphological characters can efficiently discriminate among host-associated groups based on size variation and clearly separate biotypes of L. fabarum. We concluded that L. fabarum biotypes are not homogeneous morphological entities, and represent different host-associated forms.

References

  1. Autrique, A., Starý, P. & Ntahimpera, L. (1989) Biological control of pest aphids by Hymenopterous parasites in Burundi. FAO Plant Protection Bulletin, 37, 71–74.

    Baghery Matin, S., Sahragard, A. & Rasoolian, G. (2005) Some behavioural characters of Lysiphlebus fabarum (Hym: Aphidiidae) parasitizing Aphis fabae (Homo: Aphididae) under laboratory conditions. Journal of Entomology, 2(1), 64–68.

    Belshaw, R., Quicke, D.L.J., Volkl, W. & Godfray, C.J. (1999) Molecular markers indicate rare sex in a predominantly asexual parasitoid wasp. Evolution, 53, 1189–1199.

    Billah, M.K., Kimani-Njogu, S.W., Wharton, R.A., Woolley, J.B. & Masiga, D. (2008) Comparison of five allopatric fruit fly parasitoid populations (Psyttalia species) (Hymenoptera: Braconidae) from coffee fields using morphometric and molecular methods. Bulletin of Entomological Research, 98, 63–75.

    Brewer, M.J. & Elliott, N.C. (2004) Biological control of cereal aphids in North America and mediating effects of host plant and habitat manipulations. Annual Review of Entomology, 49, 219–242.

    Carver, M. & Franzmann, B. (2001) Lysiphlebus Foerster (Hymenoptera: Braconidae: Aphidiinae) in Australia. Australian Journal of Entomology, 40, 198–201.

    Chishti, M.J.K. & Quicke, D.L.J. (1996) A revision of the Indo-Australian species of Stenobracon (Hymenoptera: Braconidae) parasitoids of lepidopterous stem borers of graminaceous crops. Bulletin of Entomological Research, 86, 227–245.

    Claridge, M.F. & Den Hollander, J. (1983) The biotype concept and its application to insect pests of agriculture. Crop Production, 2(1), 85–95.

    Diehl, S.R. & Bush, G.L. (1984) An evolutionary and applied perspective of insect biotype. Annual Review of Entomology, 29, 471–504.

    Dolphin, K. & Quicke, D.L.J. (2001) Estimating the global species richness of an incompletely described taxon: an example using parasitoid wasps (Hymenoptera: Braconidae). Biological Journal of the Linnean Society, 73, 279–286.

    Gärdenfors, U. (1986) Taxonomic and biological revision of palearctic Ephedrus Haliday (Hymenoptera: Braconidae, Aphidiinae). Entomologia Scandinavica, 27, 1–95.

    Gauld, I.D. (1986) Taxonomy, its limitations and its role in understanding parasitoid biology. In: Waage, J. & Greathead, D.J. (Eds), Insect parasitoids. Academic Press, London, pp. 1–21.

    Hagvar, E.B. & Hofsvang, T. (1991) Aphid parasitoid (Aphidiidae) biology, host selection and use in biological control. Biocontrol News and information, 12(1), 13–42.

    Höller, C. (1991) Evidence for the existence of a species closely related to the cereal aphid parasitoid Aphidius rhopalosiphi De Stefani-Perez based on host ranges, morphological characters, isoelectric focusing banding patterns, cross-breeding experiments and sex phromone specificities (Hymenoptera: Braconidae, Aphidiinae). Systematic Entomology, 16, 15–28.

    Kambhampati, S., Voelkl, W. & Mackauer, M. (2000) Phylogenetic relationships among genera of Aphidiinae (Hymenoptera: Braconidae) based on DNA sequence of the mitochondrial 16S rRNA gene. Systematic Entomology, 25, 437–445.

    Kavallieratos, N.G., Lykouressis, D.P., Sarlis, G.P., Stathas, G.J., Sanchis Segovia, A. & Athanassiou, C.G. (2001) The Aphidiinae (Hymenoptera: Ichneumonoidea: Braconidae) of Greece. Phytoparasitica, 29, 306–340.

    Kavallieratos, N.G., Tomanović, Ž., Starý, P., Athanassiou, C.G., Sarlis, G.P., Petrović, O., Niketić, M. & Veroniki, M.A. (2004) A survey of aphid parasitoids (Hymenoptera: Braconidae: Aphidiinae) of Southeastern Europe and their aphid-plant associations. Applied Entomology and Zoology, 39, 527–563.

    Kenis, M. & Mills, N.J. (1998) Evidence for the occurrence of sibling species in Eubazus spp. (Hymenoptera: Braconidae), parasitoids of Pissodes spp. weevils (Coleoptera: Curculionidae). Bulletin of Entomological Research, 88, 149–163.

    Kimani-Njogu, S.W., Overholt, W.A., Wooley, J. & Walker, A. (1997) Biosystematics of the Cotesia flavipes species complex (Hymenoptera: Braconidae): morphometrics of selected allopatric populations. Bulletin of Entomological Research, 87, 61–66.

    Kimani-Njogu, S.W., Trostle, M.K., Wharton, R.A., Wooley, J. & Raspi, A. (2001) Biosystematics of the Psyttalia concolor species complex (Hymenoptera: Braconidae: Opiinae): the identity of populations attacking Ceratitis capitata (Diptera: Tephritidae) in coffee in Kenya. Biological Control, 20, 167–174.

    Levie, A., Legrand, M.A., Dogot, P., Pels, C., Baret, P.V. & Hance, T. (2005) Mass releases of Aphidius rhopalosiphi (Hymenoptera: Aphidiinae), and strip management to control of wheat aphids. Agricultue, Ecosystems & Environment, 105, 17–21.

    Mackauer, M. (1961) Die Typen der Unterfamilie Aphidiinae des Britischen Museums London (Hymenoptera: Braconidae). Beiträge zur Entomologie, 11, 96–154.

    Mackauer, M. (1968) Aphididae. In: Ferrière, C. & Vechat, J.V.D. (Eds.), Hymenopterorum Catalogus (new edition), Part 3. Junk, The Hague, pp. 1–103.

    Mackauer, M. & Starý, P. (1967) Hymenoptera. Ichneumonoidea, World Aphidiidae. In: Delucchi, V. & Remaudière, G. (Eds.), Index of Entomophagous Insects. Le Francois, Paris, pp. 1–167.

    Manly, B.F.J. (1986) Multivariate Statistical Methods - A Primer. Chapman & Hall Ltd., London, 215 pp.

    Mescheloff, E. & Rosen, D. (1988) Biosystematic studies on the Aphidiidae of Israel (Hymenoptera: Ichneumonoidea) 1. Introduction and key to genera. Israel Journal of Entomology, 22, 61–73.

    Mescheloff, E. & Rosen, D. (1990) Biosystematic studies on the Aphidiidae of Israel (Hymenoptera: Ichneumonoidea). 3. The genera Adialytus and Lysiphlebus. Israel Journal of Entomology, 24, 35–50.

    Pennacchio, F. (1989) The Italian species of the genus Aphidius Nees (Hymenoptera, Braconidae, Aphidiinae). Bollettino del Laboratorio di Entomologia Agraria ‘Filippo Silvestri’, 46, 75–106.

    Pungerl, N.B. (1986) Morphometric and electrophoretic study of Aphidius species (Hymenoptera; Aphididae) reared from a variety of aphid hosts. Systematic Entomology, 11, 327–354.

    Rakhshani, E. (2006) A morphological and phylogenetic study of aphid parasitoids (Hym., Braconidae, Aphidiinae) using 28S r DNA gene. Ph.D thesis. Tarbiat-Modares University, Tehran, Iran.

    Reyment, R.A., Blakith, R.E. & Campbell, N.A. (1984) Multivariate Morphometrics, 2nd Ed. Academic Press, London, 233 pp.

    Roth, V.L. & Mercer, J.M. (2000) Morphometrics in development and evolution. American Zoology, 40, 801–810.

    Sanchis, A., Latorre, A., Gonzalez-Candelas, F. & Michelena, J.M. (2000) An 18S rDNA-based molecular phylogeny of Aphidiinae (Hymenoptera: Braconidae). Molecular Phylogenetics and Evolution, 2, 180–194.

    Sandrock, C., Frauenfelder, N., Von Burg, S. & Vorburger, C. (2007) Microsatellite DNA markers for the aphid parasitoid Lysiphlebus fabarum and their applicability to related species. Molecular Ecology Notes, 7, 1080–1083.

    Schmidt, M.H., Lauer, A., Purtauf, T., Thies C., Schaefer, M. & Tscharntke, T. (2003) Relative importance of predators and parasitoids for cereal aphid control. Proceedings of the Royal Society London (B) Biological Sciences, 270, 1905–1909.

    Sneath, P.H.A. & Sokal, R.R. (1973) Numerical taxonomy: the principles and practice of numerical classification. Freeman, San Francisco, 573 pp.

    Sokal, R.R. & Rohlf, F.J. (1981) Biometry. The principles and practice of statistics in biological research. W. H. Freeman and Company, San Francisco, 857 pp.

    Starý, P. (1962) Notes on the European species of the genus Ephedrus Haliday (Hymenoptera: Aphidiidae). Opuscula Entomologica, 27, 87–98.

    Starý, P. (1970) Biology of aphid parasites (Hymenoptera: Aphidiidae) with respect to integrated control. W. Junk, b. v., The Hague, 643 pp.

    Starý, P. (1971) New aphid parasites from Central Europe (Hymenoptera: Aphidiidae). Acta Entomologica Bohemoslovaca, 68, 310–318.

    Starý, P. (1979) Aphid parasites (Hymenoptera, Aphidiidae) of the Central Asian area. Trascation of Czechoslovak Academy of Science, 89, 1–116.

    Starý, P. (1985) Two new Lysiphlebus species from Europe (Hymenoptera, Aphidiidae). Acta Entomologica Bohemoslovaca, 82, 426–430.

    Starý, P. (1988) Aphidiidae. In: Minks, A.K. & Harewijn, P. (Eds.), Aphids, their biology, natural enemies and control. Elsevier Publisher, Amesterdam, Vol. B, pp. 171–184.

    Starý, P. (1993) Alternative host and parasitoid in first method in aphid pest management in glasshouses. Journal of Applied Entomology, 116, 187–191.

    Starý, P. (1999) Biology and distribution of microbe-associated thelytokous population of aphid parasitods (Hym., Braconidae, Aphidiinae). Journal of Applied Entomology, 123, 231–235.

    Starý, P. & Remaudière, G. (1973) Some aphid parasites (Hymenoptera: Aphidiidae) from Spain. Entomophaga, 18, 287–290.

    Starý, P., Havelka, J. & Choi, J.Y. (2002) New species and populations of Lysiphlebus Foerster-Aphid parasitoids (Hymenoptera: Braconidae: Aphidiinae) in Korea. Insecta Koreana, 19(3–4), 205–211.

    Starý, P., Naumann-Etienne, K. & Remaudiere, G. (1998) A review and tritrophic associations of aphid parasitoids (Hym., Braconidae, Aphidiinae) of Pakistan. Parasitica (Gembloux), 54, 3–21.

    Starý, P., Remaudière, G., González, D. & Shahrokhi, S. (2000) A review and host associations of aphid parasitoids (Hymenoptera: Braconidae: Aphidiinae) of Iran. Parasitica, 56(1), 15–41.

    Tabachnick, B.G. & Fidell, L.S. (2006) Using multivariate statistics (5th ed.). Allyn and Bacon, New York, 1008 pp.

    Thies, C., Roschewitz, I. & Tscharntke, T. (2005) The landscape context of cereal aphid-parasitoid interactions. Proceedings of the Royal Society (B), 272, 203–210.

    Tomanović, Ž., Starý, P. & Petrović, O. (2002) Monoctonus leclanti sp. n. (Hymenoptera: Braconidae: Aphidiinae) from high-montane areas of the southeastern Europe and key to related species. Entomologica Fennica, 13, 159–162.

    Tomanović, Ž., Kavallieratos, N.G., Starý, P., Stanisavljević, L.Ž., Ćetković, A., Stamenković, S., Jovanović, S. & Athanassiou, C.G. (2009) Regional tritrophic relationship patterns of five aphid parasitoid species (Hymenoptera: Braconidae: Aphidiinae) in agroecosystem-dominated landscapes of Southeastern Europe. Journal of Economic Entomology, 102(3), 836–854.

    Tomić, M., Tomanović, Ž., Kavallieratos, N.G., Starý, P., Athanassiou, C.G., Tomić, V. & Lučić, L. (2005) Morphological variability of several biotypes of Ephedrus plagiator (Nees, 1811) (Hymenoptera: Braconidae: Aphidiinae) and description of a new species. Zoologischer Anzeiger, 244, 153–162.

    Villemant, C., Simbolotti, G. & Kenis, M. (2007) Discrimination of Eubazus (Hymenoptera: Braconidae) sibling species using geometric morphometrics analysis of wing venation. Systematic Entomology, 32, 625–634.

    Žikić, V., Tomanović, Ž., Ivanović, A., Kavallieratos, N.G., Starý, P., Stanisavljević, L.Ž. & Rakhshani, E. (2009) Morphologicl characterization of Ephedrus persicae biotypes (Hymenoptera: Braconidae: Aphidiinae) in the Palaearctic. Systematics, 102(1), 1–11.