Skip to main content Skip to main navigation menu Skip to site footer
Type: Articles
Published: 2011-01-18
Page range: 27–39
Abstract views: 69
PDF downloaded: 37

Exploring unknown life stages of Arctic Tanytarsini (Diptera: Chironomidae) with DNA barcoding

Museum of Natural History and Archaeology, Norwegian University of Science and Technology, NO-7491 Trondheim, Norway
Museum of Natural History and Archaeology, Norwegian University of Science and Technology, NO-7491 Trondheim, Norway
Diptera Chironomids immature stages descriptions keys Svalbard

Abstract

We present the first results from a project on Spitsbergen and Bear Island where the ultimate goal is to provide genetic and morphological identification keys to all species. Five Tanytarsini species have been recorded from Svalbard. By sequencing partial COI gene sequences, we have associated larvae of all of these, and describe the hitherto undescribed larval stages of Microspectra insignilobus Kieffer, M. radialis Goetghebuer and Tanytarsus heliomesonyctios Langton. We also present keys to larva, pupa and imagines of all Tanytarsini species found on Svalbard and discuss larval morphology in relationship to characters preserved in subfossil material. Of particular interest is the mandible and mentum of T. heliomsonyctios which show close resemblance to the tentatively associated larvae of Corynocera oliveri Lindeberg. An overview over Tanytarsus species with “lugens-type” larvae is given and the phylogenetic value of mandibular accessory teeth is briefly discussed.

References

  1. Boheman, C.H. (1865) Spetsbergens Insekt-Fauna. Öfversigt af Kongliga Vetenskaps-Akademiens Förhandlingar, 22, 563–577.

    Brooks, S.J., Langdon, P.G. & Heiri, O. (2007) The identification and use of Palaearctic Chironomidae larvae in paleoecology. QRA Technical Guide No. 10, Quaternary Research Association, London, 276 pp.

    Brundin, L. (1949) Chironomiden und andere Bodentiere der südschwedischen Urgebirgsseen. Ein Beitrag zur Kenntnis der bodenfaunistischen Charakterzüge schwedischer oligotropher Seen. Drottningholm, Lund: Institute of freshwater research, 914 pp.

    Carew, M.E., Pettigrove, V. & Hoffmann, A.A. (2005) The utility of DNA markers in classical taxonomy: Using cytochrome oxidase I markers to differentiate Australian Cladopelma (Diptera: Chironomidae) midges. Annals of the Entomological Society of America, 98, 587–594.

    Coulson, S. & Refseth, D. (2004) Chapter 3. The terrestrial and freshwater invertebrate fauna of Svalbard (and Jan Mayen). In: Prestrud, P., Strøm, H. & Goldman, H. V. (Eds.) A catalogue of the terrestrial and marine animals of Svalbard. Norwegian Polar Institute, Skrifter 201, pp. 57 – 122.

    Edwards, F.W. (1922) Results of the Oxford University Expedition to Spitsbergen, 1921. – No. 14, Diptera Nematocera. The Annals and magazine of natural history Series 9, 10, 193–215.

    Edwards, F.W. (1924) Results of the Merton College Expedition to Spitsbergen, 1923. – No. 14, Diptera Nematocera. The Annals and magazine of natural history Series 9, 14, 162–174.

    Edwards, F.W. (1925) Diptera (Nematocera) from Spitsbergen. Results of the Oxford University Expedition to Spitsbergen, 1921. The Annals and magazine of natural history Series 9, 16, 354–356.

    Edwards, F.W. (1935) Diptera from Bear Island. The Annals and magazine of natural history Series 10, 5, 531–543.

    Ekrem, T. (2003) Towards a phylogeny of Tanytarsus van der Wulp (Diptera: Chironomidae). Is morphology alone sufficient to reconstruct the genealogical relationship? Insect Systematics and Evolution, 34, 199–219.

    Ekrem, T., Stur, E. & Hebert, P.D.N. (2010) Females do count: Documenting Chironomidae (Diptera) species diversity using DNA barcoding. Organisms Diversity & Evolution, 10, 397–408.

    Ekrem, T., Willassen, E. & Stur, E. (2007) A comprehensive DNA sequence library is essential for identification with DNA barcodes. Molecular Phylogenetics and Evolution, 43, 530–542.

    Epler, J.H. (2001) Identification Manual for the Larval Chironomidae (Diptera) of North and South Carolina. North Carolina Department of Environment and Natural Resources and St. Johns River Water Management District, Special Publication SJ2001-SP13. Raleigh, North Carolina, p. 526.

    Goetghebuer, M. (1933) Chironomides du Groenland oriental, du Svalbard et de la Terre de François Joseph. Skrifter om Svalbard og Ishavet, 53, 19–31.

    Goetghebuer, M. (1939) Ceratopogonidae et Chironomidae nouveaux ou peu connus d’Europe (Dixième Note). Bulletin et Annales de la Société Entomologique de Belgique, 79, 379–389.

    Hebert, P.D.N., Penton, E.H., Burns, J.M., Janzen, D.H. & Hallwachs, W. (2004) Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences of the United States of America, 101, 14812–14817.

    Hofmann, W. (1984) A subfossil record of the presumed larva of Corynocera oliveri Lindeberg from the Lobingsee (Swiss Plateau). Studies in the Late-Quaternary of Lobingsee 8. Spixiana, 7, 211–214.

    Holmgren, A.E. (1869) Bidrag til Kännodomen om Beeren Eilands och Spetsbergens Insekt-Fauna. Kungliga Svenska vetenskapsakademiens handlingar, 8, 1–55.

    Kieffer, J.J. & Thienemann, A. (1919a) Chironomiden, gesammelt von Dr. A. Koch (Münster i. W.) auf den Lofoten, der Bäreninsel und Spitzbergen (Dipt.). Entomologische Mitteilungen, 8, 38–48.

    Kieffer, J.J. & Thienemann, A. (1919b) Chironomiden, gesammelt von Dr. A. Koch (Münster i. W.) auf den Lofoten, der Bäreninsel und Spitzbergen (Dipt.). Entomologische Mitteilungen, 8, 110–124.

    Kieffer, J.J. (1924) Quelques nouveaux chironomides de Scandinavie. Annales de la Societé scientifique de Bruxelles, 44, 80–86.

    Klink, A.G. (1983) Key to the Dutch larvae of Paratanytarsus Thienemann & Bause with a note on the ecology and the phylogenetic relations. Medeklinker, 3, 1–36.

    Langton, P.H. (1998) [distributed 1999] Micropsectra silvestrae n. sp. and Tanytarsus heliomesonyctios n. sp., (Diptera: Chironomidae), Two parthenogenetic species from Ellesmere Island, Arctic Canada. Journal of the Kansas Entomological Society, 71, 208–215.

    Laville, H. & Vilchez-Quero, A. (1986) Les Chironomidés (Diptera) de quelques “lagunas” de haute altitude de la Sierra Nevada (Granada, Espagne). Annales de Limnologie, 22, 53–63.

    Norwegian Polar Institute (2009) Bjørnøya. Norwegian Polar Institute. Available from: http://npweb.npolar.no/geografi/bjornoya (September 25, 2009).

    Oliver, D.R. (1962) A review of the subfamily Orthocladiinae (Chironomidae, Diptera) of Bear Island. Astarte, 20, 1–19.

    Pinder, L.C.V. & Reiss, F. (1983) 10. The larvae of Chironominae (Diptera: Chironomidae) of the Holarctic region. – Keys and diagnoses. In: Wiederholm, T. (Ed.) Chironomidae of the Holarctic region. Keys and diagnoses. Part 1. Larvae. Entomologica scandinavica Supplement, 19, pp. 293–435.

    Pinder, L.C.V. & Reiss, F. (1986) 10. The pupae of Chironominae (Diptera: Chironomidae) of the Holarctic region. – Keys and diagnoses. In: Wiederholm, T. (Ed.) Chironomidae of the Holarctic region. Keys and diagnoses. Part 2. Pupae. Entomologica scandinavica Supplement, 28, pp. 299–456.

    Prestrud, P., Strøm, H. & Goldman, H.V. (2004) Introduction. In: Prestrud, P., Strøm, H. & Goldman, H. V. (Eds.) A catalogue of the terrstrial and marine animals of Svalbard. Norwegian Polar Institute, Skrifter 201, pp. 1–4.

    Przhiboro, A., Ekrem, T. & Stur, E. (in press) Bionomics and taxonomy of Tanytarsus recurvatus Brundin, 1947. Wang, X. et al. (Eds.) Proceedings of the 17th International Symposium on Chironomidae.

    Ratnasingham, S. & Hebert, P.D.N. (2007) BOLD: The Barcode of Life Data System (www.barcodinglife.org). Molecular Ecology Notes, 7, 355–364.

    Reiss, F. & Säwedal, L. (1981) Keys to males and pupae of the Palaearctic (excl. Japan) Paratanytarsus Thienemann & Bause, 1913, n. comb., with descriptions of three new species (Diptera: Chironomidae). In: Cederholm, L. (Ed.) Advances in insect systematics and phylogeny. Entomologica scandinavica Supplement, 15, 73–104.

    Sæther, O.A. (1980) Glossary of chironomid morphology terminology (Diptera: Chironomidae). Entomologica scandinavica Supplement, 14, 1–51.

    Säwedal, L. (1976) Revision of the notescens-group of the genus Micropsectra Kieffer, 1909 (Diptera: Chironomidae). Entomologica scandinavica, 7, 109–144.

    Schnell, Ø.A. (1998) Guidelines for identification of chironomid larvae in the MOLAR project, NIVA Report, SNO 3710–97, Norwegian Institute for Water Research, 23 pp.

    Stur, E. & Ekrem, T. (2006) A revision of West Palaearctic species of the Micropsectra atrofasciata group (Diptera: Chironomidae). Zoological Journal of the Linnean Society, 146, 165–225.

    Sublette, J.E. (1964) Chironomidae (Diptera) of Lousiana I. Systematics and immature stages of some lentic chironomids of West-Central Lousiana. Tulane Studies in Zoology, 11, 109–150.

    Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007) MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599.