Abstract
Two new species of the East African annual killifish genus Nothobranchius, N. krysanovi and N. pienaari, occurring on the coastal plains of Mozambique and in the Republic of South Africa, are described. Both are members of a clade, herein termed as N. rachovii species group, within the subgenus Nothobranchius. The species N. rachovii is rediagnosed on the basis of cytology, mtDNA analyses, morphology and male colouration. Both new species, N. krysanovi and N. pienaari, can be distinguished from the other species of the group by male colouration, morphology, cytology and mtDNA sequences. Based on mtDNA analyses the subgenus Zononothobranchius is reviewed and presently regarded as containing only one species.
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