Abstract
Among the two most widely distributed duiker species, Philantomba monticola (Thunberg, 1789) and Philantomba maxwelli (C. H. Smith, 1827), the latter shows geographic variation in pelage color and body size. This issue was not investigated in detail so far, especially in the eastern region of its distribution area, notably due to the lack of material from the Dahomey Gap. We undertook a species-level revision of Philantomba in West Africa, notably including a series of specimens collected in Togo, Benin and Nigeria. Using morphological measurements (craniometry) and genetic data (two mitochondrial and three nuclear markers), we describe a new duiker species occurring in the Dahomey Gap (Togo, Benin) and the Niger delta, Philantomba walteri sp. nov. This discovery highlights the importance of the Dahomey Gap for the evolutionary history of the West African forest faunas. It also has conservation implications given that the new species is one of the main targets of the local bushmeat trade.References
Bennett, E.L., Blencowe, E., Brandon, K., Brown, D., Burn, R.W., Cowlishaw, G., Davies, G., Dublin, H., Fa, J.E., Milner-Gulland, E.J., Robinson, J.G., Rowcliffe, J.M., Underwood, F.M. & Wilkie, D.S. (2007) Hunting for consensus: reconciling bushmeat harvest, conservation, and development policy in West and Central Africa. Conservation Biology, 21, 884–887.
Brashares, J.S., Arcese, P., Sam, M.K., Coppolillo, P.B., Sinclair, A.R. & Balmford, A. (2004) Bushmeat Hunting, Wildlife Declines, and Fish Supply in West Africa. Science 12, 306 (5699), 1180–1183.
Colyn, M., Dudu, A. & Mankoto, M.S. (1987) Données sur l’exploitation du petit et moyen gibier: 1. Consommation qualitative dans le milieu rural ; 2. Commercialisation du gibier dans les centres urbains. In: Symposium International sur la gestion de la faune en Afrique sub-saharienne. Harare, Zimbabwe, International Foundation for the Conservation of Game, pp. 109–145.
Eves H.E. & Ruggiero, R.G. (2000) Socioeconomics and the sustainability of hunting in the forests of northern Congo (Brazzaville). In: Robinson J.G. & Bennett E.L. (Eds), Hunting for sustainability in tropical forests. Colombia University Press, pp. 427–454.
Fimbel, C., Curran, B. & Usongo, L. (2000) Enhancing the sustainability of duiker hunting through community participation and controlled access in the Lobéké Region of the southeastern Cameroon. In: Robinson, J.G. & Bennett, E.L. (Eds), Hunting for sustainability in tropical forests. Colombia University Press, pp. 356–375.
Folmer, O., Black, M., Hoeh, R., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology,3, 294–299.
Grubb, P. (2005) Artiodactyla. In; Wilson, D.E. & Reeder, D.M. (Eds), Mammal Species of the World. A Taxonomic and Geographic Reference (3rd edition). Johns Hopkins University Press, Baltimore, pp. 637–722.
Grubb, P. & Groves, C.P. (2005) Revision and classification of the Cephalophinae. In: Wilson V. J., (Ed), Duikers and rainforests of Africa. Chipangali Wildlife Trust, Ascot, Bulawayo, Zimbabwe, pp. 703–728.
Happold, D.C.D. (1987) The Mammals of Nigeria. Clarendon Press, Oxford, 402 pp.
Hebert, P.D.N., Ratnasingham, S. & de Waard, J.R. (2003) Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society of London B (Supplement), 270, S96–S99.
Helm-Bychowski, K. & Cracraft, J. (1993) Recovering phylogenetic signal from DNA sequences: Relationships within the corvine assemblage (Class: Aves) as inferred from complete sequences of the mitochondrial DNA cytochrome b gene. Molecular Biology and Evolution, 10, 1196–1214.
IUCN SSC Antelope Specialist Group (2009) One fourth of antelope species are threatened with extinction in the World. Available from http://cmsdata.iucn.org/downloads/antelope_report.pdf (2 July 2010)
Jansen van Vuuren, B. & Robinson, T.J. (2001) Retrieval of four adaptive lineages in duiker antelope: Evidence from mitochondrial DNA sequences and fluorescence in situ hybridization. Molecular Phylogenetics and Evolution, 20, 409–425.
Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120.
Kocher, T.D., Thomas, W.K., Meyer, A., Edwards, S.V., Pääbo, S., Villablanca, F.X. & Wilson, A.C. (1989) Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proceedings of the National Academy of Sciences USA, 86, 6196–6200.
Matthee, C.A., Burzlaff, J.D., Taylor, J.F. & Davis, S.K. (2001) Mining the mammalian genome for artiodactyl systematics. Systematic Biology, 50, 367–390.
Ntie, S., Johnson, A., Mickala, P., Bowkett, A., van Vuuren, B., Colyn, M., Telferg, P., Maisels, F., Hymas, O., Rouyer, R., LeBlanc, K., van Vliet, N., Sonet, G., Verheyen, E., Ryder, O., Wickings, J. & Anthony, N. (2010) A molecular diagnostic for identifying Central African forest artiodactyls from faecal pellets. Animal Conservation, 13, 80–93.
Olayemi, A., Nicolas, V., Gaubert, P., Leirs, H. & Verheyen, E. (in press) Small mammals, morphology and molecules: Tale-bearing tenants of the Nigerian southwestern forest block. In; 'Forests: Layers, flora, fauna and climate change. Nova Science Publishers.
Olson, D.M. & Dinerstein, E. (1998) The global 2000: a representation approach to conserving the Earth's most biologically valuable ecoregions. Conservation Biology, 12, 502.
Posada, D. (2008) jModelTest: Phylogenetic Model Averaging. Molecular Biology and Evolution, 25, 1253–1256.
Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 19, 1572–1574.
Sokal, R.R. & Rohlf, F.J. (1969) Biometry. W.H. Freeman and Company, N. York, pp. 327–332.
StatSoft Inc. (2003) STATISTICA, version 6. www.statsoft.com.
Swofford, D.L. (2002) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). (Sinauer Associates, Sunderland, Massachusetts).
Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Molecular Biology and Evolution, 24, 1596–1599.
Townsend, T.M., Alegre, R.E., Kelley, S.T., Wiens, J.J. & Reeder, T.W. (2008) Rapid development of multiple nuclear loci for phylogenetic analysis using genomic resources: an example from squamate reptiles. Molecular Phylogenetics and Evolution, 47, 129–142.
Van Vliet, N. & Nasi, R. (2008) Why do models fail to assess properly the sustainability of duiker (Cephalophus spp) hunting in Central Africa? Oryx, 42(3), 392–399.
Ward, R.D., Zemlak, T.S., Innes, B.H., Last, P.R. & Hebert, P.D.N. (2005) DNA barcoding Australia’s fish species. Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences, 360, 1847–1857.
Wilson, V.J. (2005) Duikers and rainforests of Africa. A masters of the African forest floor. Chipangali Wildlife Trust, Ascot, Bulawayo, Zimbabwe, Chipangali Wildlife Trust, 798 pp.