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Issue: Vol. 2428 No. 1: 16 Apr. 2010
Type: Articles
Published: 2010-04-16
DOI: 10.11646/zootaxa.2428.1.2
Page range: 22–36
Abstract views: 58
PDF downloaded: 40

A new black fly species from Brazil, closely related to Simulium guianense Wise (Diptera, Simuliidae), revealed by morphology and DNA barcoding

Coordenação de Pesquisas em Entomologia, Caixa Postal 478, 69011-970, Manaus, AM, Brazil
Laboratório de Entomologia Aquática, Departamento de Hidrobiologia, Universidade Federal de São Carlos, Caixa Postal 676, 13565-905, São Carlos, SP, Brazil
Coordenação de Pesquisas em Entomologia, Caixa Postal 478, 69011-970, Manaus, AM, Brazil Divisão de Curso em Genética, Conservação e Biologia Evolutiva, Instituto Nacional de Pesquisas da Amazônia, Caixa Postal 478, 69011-970, Manaus, AM, Brazil
Centro de Pesquisa Leônidas e Maria Deane - Biodiversidade em Saúde, Fundação Oswaldo Cruz, Rua Teresina n. 476, Adrianópolis, 69.057-070, Manaus, AM, Brazil
Diptera aquatic insects taxonomy COI genetic distance sibling species

Abstract

The male, female, pupa and larva of Simulium litobranchium n. sp. are described and illustrated. This new species has 12 gill filaments, as do S. duodenicornium Pepinelli, Hamada & Trivinho-Strixino, S. guianense Wise, S. hirtipupa Lutz, S. perplexum Shelley, Maia-Herzog, Lunas Dias & Couch and S. scutistriatum Lutz, but it can be distinguished from these other species by a combination of characters observed at the pupal and adult stages. DNA barcoding showed that Simulium litobranchium n. sp. exhibits more than 4% nucleotide divergence in cytochrome oxidase I from three other closely related species in the Neotropical subgenus S. (Thyrsopelma). The new species was collected in the Brazilian states of Goiás and Minas Gerais and inhabits rivers 30–40 m in width in the Paraná River hydrographic basin. Females were not observed engaging in anthropophilic behavior.

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