Abstract
Myotis flavus, a synonym of M. formosus found in Taiwan, is reported for the first time for mainland China. We captured one bat in Jiangxi province in Southeast China and identified the taxon as M. flavus based upon its external, cranial and baculum morphology. The uncorrected cytb sequence divergence between M. flavus from Jiangxi and specimen from Taiwan was only 0.61%, indicating intraspecific divergence. Both, morphological and genetic evidence indicated that the specimen from mainland China was a new locality record of M. flavus. In contrast, M. flavus from Jiangxi and from Taiwan exhibited 15.53–16.67% sequence divergence from samples of M. formosus obtained from locations outside Taiwan, and they were not sister lineages. Therefore M. flavus should be recognized as a species from M. formosus. In addition, the sequence similarity between M. watasei from Taiwan and M. formosus from mainland China and their phylogenetic grouping strongly suggests that M. watasei is a synonym of M. formosus.References
Baker, R.J. & Bradley, R.D. (2006) Speciation in mammals and the genetic species concept. Journal of Mammalogy, 87, 643–662.
Bates, P.J. & Harrison, D. (1997) Bats of the Indian subcontinent. Harrison Zoological Museum, Sevenoaks, pp 122–125.
Bickford, D., Lohman, D.J., Sodhi, N.S., Ng, P.K.L., Meier, R., Winker, K., Ingram, K.K. & Das, I. (2007) Cryptic species as a window on diversity and conservation. Trends in Ecology and Evolution, 22, 148–155.
Chou, C.H. (2004) Taxonomic status of the genus Myotis (Chiroptera: Vespertilionidae) in Taiwan, p. 115. Vol. master. Tunghai university, Taichung
Corbet, G.B. & Hill, J.E. (1992) The Mammals of the Indomalayan Region. Natural History Museum Publications and Oxford University Press, Oxford, 488 pp.
Findley, J.S. (1972) Penetic relationships among bats of the Genus Myotis. Systematic Zoology, 21, 31–52.
Gascuel, O. (1997) BIONJ: an improved version of the NJ algorithm based on a simple model of sequence data. Molecular Phylogenetics and Evolution, 14, 685–95.
Guindon, S. & Gascuel, O. (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systems Biology, 52, 696–704.
Heaney, L.R., Balete, D.S., Dolar, M.L., Alcala, A.C., Dans, A.T.L., Gonzales, P.C. Ingle, N.R., Lepiten, M.V., Oliver, W.L.R., Ong, P.S., Rickart, E.A., Tabaranza, Jr., B.R. & Utzurrum, R.C.B. (1998) A synopsis of the mammalian fauna of the Philippine Islands. Fieldiana: Zoology, new series, 88, 1–61.
Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17, 754–755.
Irwin, D.M., Kocher, T.D. & Wilson, A.C. (1991) Evolution of the cytochrome b gene of mammals. Journal of Molecular Evolution, 32, 128–144.
Jones, G., Parsons, S., Zhang, S., Stadelmann, B., Benda, P. & Ruedi, M. (2006) Echolocation calls, wing shape, diet and phylogenetic diagnosis of the endemic Chinese bat Myotis pequinius. Acta Chiropterologica, 8, 451–463.
Kawai, K., Nikaido, M., Harada, M., Matsumura, S., Lin, L.K., Wu, Y., Hasegawa, M. & Okada, N. (2003) The status of the Japanese and East Asian bats of the genus Myotis (Vespertilionidae) based on mitochondrial sequences. Molecular Phylogenetics and Evolution, 28, 297–307.
Kocher, T.D., Thomas, W.K., Meyer, A., Edwards, S.V., Paabo, S., Villablanca, F.X. & Wilson, A.C. (1989) Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proceedings of the National Academy of Sciences of United States of America, 86, 6196–200.
Lin, L.K., Lee, L.L. & Cheng, H.C. (2005) Bats of Taiwan. National Museum of Natural Science, Taiwan, 107–114.
Posada, D. & Crandall, K.A. (1998) MODELTEST: Testing the model of DNA substitution. Bioinformatics, 14, 817–818.
Ruedi, M. & Mayer, F. (2001) Molecular systematics of bats of the genus Myotis (Vespertilionidae) suggests deterministic ecomorphological convergences. Molecular Phylogenetics and Evolution, 21, 436–448.
Sakai, T., Kikkawa, Y., Tsuchiya, K., Harada, M., Kanoe, M., Yoshiyuki, M. & Yonekawa, H. (2003) Molecular phylogeny of Japanese Rhinolophidae based on variations in the complete sequence of the mitochondrial cytochrome b gene. Genes and Genetic Systems, 78, 179–189.
Sara, B., Harrison, D.L., Satasook, C., Prajukjitr, A., Thong-Aree, S. & Paul, B. (2006) A review of bat research in Thailand with eight new species records for the country. Acta Chiropterologica, 8, 325–329.
Shamel, H.H. (1944) A new Myotis from Formosa. Journal of Mammalogy, 25, 191–192.
Simmons, N.B. (2005) Order Chiroptera. In: Mammal species of the World: a taxonomic and geographic reference. Johns Hopkins University Press, Baltimore, 2142 pp.
Stadelmann, B., Herrera, L.G., Arroyo-Cabrales, J., Flores-Martinez, J.J., May, B.P. & Ruedi, M. (2004a) Molecular systematics of the fishing bat Myotis (Pizonyx) vivesi. Journal of Mammalogy, 85, 133–139.
Stadelmann, B., Jacobs, D.S., Schoeman, C. & Ruedi, M. (2004b) Phylogeny of African Myotis bats (Chiroptera, Vespertilionidae) inferred from cytochrome b sequences. Acta Chiropterologica, 6, 177–192.
Stadelmann, B., Lin, L.K., Kunz, T.H. & Ruedi, M. (2007) Molecular phylogeny of New World Myotis (Chiroptera, Vespertilionidae) inferred from mitochondrial and nuclear DNA genes. Molecular Phylogenetics and Evolution, 43, 32–48.
Swofford, D.L. (2002) PAUP*. Phylogenetic analysis using parsimony (* and other methods). Beta version 4.0b4a. In: Sinauer Associates, Inc., Publishers, Sunderland, Massachusetts.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876–82.
Wu, Y., Motokawa, M. & Harada, M. (2008) A New Species of Horseshoe Bat of the Genus Rhinolophus from China (Chiroptera: Rhinolophidae). Zoological Science, 25, 438–443.
Yoshiyuki, M. (1989) A Systematic Study of the Japanese Chiroptera. National Science Museum monographs, 242 pp.